Background: About 8 million cases of Tuberculosis occurs annually. With 2.5 million cases occurring in India, making it one of the high burden countries. Most of the cases go undetected resulting in a high mortality rate. With the launch of RNTCP and DOTS the awareness, diagnosis and treatment has increased considerably. Interns being the first contacts as health care providers for patients, must be sensitised to the latest diagnosis and treatment modalities. Objectives: To assess the awareness and knowledge of interns in the tertiary hospital regarding the TB disease, RNTCP and DOTS guidelines. Materials and Method: A cross sectional study was conducted among the interns of a medical college using a pre-validated, per-tested, structured questionnaire. 115 interns participated in the study. Frequencies and percentages of data collected was analysed. Results: The knowledge regarding TB, Diagnosis, RNTCP and DOTS and definitions were found to be 90.85%, 94.8%, 70.96%, 82.6% respectively. Out of which the knowledge was found to be 39.25%, 40.5%, 30.62% and 34.5% respectively among males and 51.1%, 54.2%, 40.34%, 48% respectively among females. Also, it was found that the knowledge was 67.6%, 69.5%, 51.84%, 60.8% respectively among the age group of 20-22 years and 23.9%, 25.2%, 19.14%, 21.7% among 23-25 years respectively. Conclusion: This study reveals that the awareness and knowledge of interns regarding TB disease, RNTCP and DOTS were fairly good. Further enhancement of knowledge on recent updates, guidelines and treatment is necessary