Introduction Laryngovideostroboscopy (LVS) is the gold standard in diagnosis of the larynx, but the interpretation of the tool is highly dependent on the examiner’s competence and knowledge. An objective measure of LVS, so far used only subjectively, provides a tool for precise diagnosis of laryngeal diseases. Aim The aim of the study was to test two different types of videostrobokymographic (VSK) software – Divas and DiagnoScope. We also compared measures of Open Quotient obtained using the two programs. Material and Methods 23 LVS recordings from a group of euphonic people were analyzed with Divas software (Xion Medical) and DiagnoScope Specialist software (DiagNova Technologies). Results The mean age in the study group was 41.1 years (women: 39.6; men: 42.4). The mean OQVSK value averaged from all three parts of the glottis measured by Divas was 0.519 and by DiagnoScope, 0.517. A Student t-test showed no significant differences between the parameters. For all measurements, absolute differences were less than 0.18. In 50% of patients, the differences in measurements did not exceed 0.05 and in 87%, 0.1. The largest differences were observed in the anterior part of the vocal folds. Conclusions The average values of OQVSK measurements are comparable despite the use of different measurement software. Software available on the market with a VSK module requires fine-tuning to limit the recording analysis time as much as possible.