Average Swimming Research Articles

Effects of a Single and Course Application of Remote Ischemic Preconditioning on the Physical Performance of Laboratory Animals

Previous publications reported the use of remote ischemic preconditioning (rIPC) as a non-drug method for increasing physical performance. However, the reported data are of a contradictory nature. Aim: to evaluate the potential of rIPC for increasing the physical performance of laboratory animals. The study was performed on 72 white outbred male rats. An assessment of the physical performance was carried out using a modified Porsolt test. The hind limbs of non-anesthetized animals were subjected to preconditioning. The results of a single and course (during 5 days) application of rIPC was evaluated. A single use of rIPC increased the average swimming time in the experimental group by 38.5%. The course application of rIPC did not lead to significant changes in the indicators of physical performance in any of the animal groups.

A comprehensive system for detection of behavioral change of D. magna exposed to various chemicals

The purpose of the present study was to develop a sensitive and comprehensive method, based on D. magna swimming behavior, for toxicity assessment of environmental chemicals. Firstly, D. magna swimming in several chambers with different diameters were compared to determine the most suitable container, and then baseline behaviors during light/dark periods as well as reactions to light/dark switching and vibration stimulation were determined. Secondly, after exposure to sub-lethal concentrations of the selected 42 typical chemicals, which were classified into heavy metals, pesticides, fungicides and flame retardants, the alterations in the swimming parameters were evaluated. Our results indicated the 48-well plate was the most suitable chamber for behavioral monitoring of D. magna, and specific responsive patterns of D. magna neonates to light/dark switching and vibration stimulation were observed. The results of the behavioral assays of chemicals suggested that D. magna was the most sensitive to methylmercury-chloride and then to abamectin and chlorpyrifos. The three chemicals at several to dozens of ng/L significantly changed swimming behaviors of D. magna. Furthermore, the alteration in the behavioral parameters (average swimming speed, etc.) induced by the selected chemicals could be ascribed to various modes of actions, confirming the reliability and practicability of the monitoring method.

Comparison of performance of various leg-kicking techniques in fin swimming in terms of achieving the different goals of underwater activities.

The aim of this study was to compare underwater fin swimming performance using dolphin, flutter and breaststroke kicks with and without diving gear. Performance was evaluated in terms of average swimming velocity. The parameters of spatiotemporal structure of the stroke reflecting to the swimming economy were employed. Conscious modifications in propulsion technique were considered here with the aim of controlling swimming performance. A total of ten professional scuba divers swam at maximal speed underwater for 50m using each of three techniques: dolphin, flutter, or breaststroke kicks. Swimmers’ performance was compared between holding their breath and using breathing apparatus. Two cameras recorded their movements in sagittal and transverse planes. The average swimming velocity (vav), stroke length (SL), stroke rate (SR), index of variation of intracycle velocity (VIVIndex) and stroke index (SI) were estimated. Relative to the other techniques, the dolphin kick without a diving gear demonstrated the highest vav and low SI and VIVIndex values, which reflects the most advantageous economy of propulsion at given velocity. Given the lack of statistical differences, using the breaststroke kick and flutter kick when swimming with a diving gear seems to be comparable to dolphin-kick in terms of average velocity and parameters reflecting the economy of propulsion. Thus, a search for fin swimming techniques with the aim of achieving specific goals seemed reasonable. The results suggest, that performance achieved while using various fin swimming techniques was probably controlled by different strategies of leg movements. These strategies revealed differences in a spatiotemporal (SR-SL) structure of the stroke and they were closely associated in terms of the velocity variation decrease.

Behaviour and ability of a cyprinid (Schizopygopsis younghusbandi) to cope with accelerating flows when migrating downstream

AbstractThe migration corridors in regulated rivers lead downstream fish migrants, particularly juveniles to pass through water infrastructure. Accelerating flow, experienced by fish, might trigger avoidance behaviour and then influence the downstream migration efficacy. It is essential to understand the causes of avoidance behaviour exhibited by downstream migratory fish in accelerating flow. In this study, the effect of three different accelerating flows on the downstream migration behaviour of Schizopygopsis younghusbandi (S.Y) was investigated using a constriction wedge in a circulating flume. The results showed that some fish (30%, 23%, and 39% under low, medium, and high flow conditions, respectively) repeatedly attempted to burst upstream with positive rheotaxis prior to successful passage downstream. Under the low‐, medium‐, and high‐accelerating levels, the average fish swimming speeds were 89.19, 91.28, and 111.94 cm/s, respectively; these values were close to the critical swimming speed (110.42 cm/s) of the target fish. The water velocities at the fish avoidance points were centrally distributed at approximately 73.03 cm/s. Regarding turbulence, the results exhibited that the S.Y generally responded to a discrete range of <50 cm2/s2 of turbulent kinetic energy and < 2 N/m2 of the horizontal component of the Reynolds shear stress (RSSxy). Also, the fish that exhibited avoidance behaviour were not centrally distributed in the lateral and longitudinal velocity locations, where there was an abrupt change in the gradient. This study highlighted the impact of accelerating flow on the downstream fish migration behaviour of a cyprinid. Furthermore, this study quantified the hydraulic factors that triggered this avoidance. Thus, it provided experimental support for optimizing the design of the hydraulic factors for downstream fishways.

Psychophysiological respond in a swimming ultra-endurance event.

Ultra-endurance events under critical environmental conditions represent unique stress, resulting in acute marked adaptations to the cardiovascular, respiratory, metabolic and neuromuscular systems of the organism. In line with this, no studies in swimming events were found thus far. This research aimed to analyze the psychophysiological response of swimmers in an ultra-endurance swimming event. Changes in the rate of perceived exertion, blood lactate concentration, 1000m swimming time and cortical arousal were measured in 19 male volunteer swimmers (28.0±5.6 years; 175.0±7.9 cm; 70.1±7.8 kg) in 3 moments during a 24h swimming event. We found a significant increase in lactate concentration after series 1, decreasing the values in series 2 and series 3. Cortical arousal decreased significantly in series 3 respect the basal sample. Rate of perceived exertion significantly increased in the swimming ultra-endurance event and 1000m swimming time was maintained during the series analyzed. An ultra-endurance swimming event produced an increase in blood lactate concentration and rated of perceived exertion and a decrease in cortical arousal, not affecting average swimming velocity along the event.

Assessment of exposure of children swimmers to trihalomethanes in an indoor swimming pool.

This study aimed to understand the exposure levels of trihalomethanes (THMs) in an indoor swimming pool and calculate the risks of exposure to THMs, based on the presence of each THM species, of children swimmers aged 6-17, in Beijing, China. We obtained exposure factors for the children through questionnaires and measured THM concentrations through laboratory tests, and we combined the results with an exposure model to calculate the risks, with consideration of different exposure routes (oral ingestion, inhalation and dermal absorption). In terms of exposure factors for the swimmers aged 6-17, the average body weight, exposure duration, exposure frequency, swimming time, shower time, changing time, warm-up exercise and rest time, skin surface area and ingestion rate of pool water were 40.46 kg, 2.70 years, 96 events/year, 64.03 min/event, 17.04 min/event, 15.31 min/event, 12.71 min/event, 1.37 m2 and 48.93 ml/event, respectively. The THM concentrations in swimming pool water, shower water, swimming pool air and locker room air were 67.17 μg/L, 12.64 μg/L, 358.66 μg/m3 and 40.98 μg/m3, respectively. The average cancer risk of THMs was 5.44 × 10-6, which is an unacceptable risk according to the United State Environmental Protection Agency (USEPA) Guidelines. The average hazard index was 0.007, i.e., less than 1, indicating that the noncancer risk was acceptable. Chloroform (TCM) was the main substance in four species of THMs and inhalation exposure was the main exposure pathway. The risk of cancer and noncancer from inhalation exposure to THMs accounts for 97-99% of the total risk. As a result, the disease control authorities and administrative department should pay attention to the health and safety of swimming facilities and, at the same time, establish standards for THMs in the air through further research.

Core Temperature While Swimming In A Wetsuit During 1000-m Race Pace Swim

Majority of fatalities that occur in the sport of triathlon happen during the swim portion of the race (Harris et al., 2010, JAMA). The potential risk of death while swimming has raised safety concerns. The governing body, USA Triathlon (USAT), has implemented guidelines related to water temperatures and the use of wetsuits. The varying water temperatures allow for a wide selection of wetsuits. Using a wetsuit while swimming in warm water may increase body heat storage which could increase core temperature. Currently, there are only limited data on the influence of triathlon wetsuits on core temperature when swimming in warm water while swimming a short distance at a somewhat hard swim pace (Aura et al., 2019, MSSE). PURPOSE: The purpose of this study was to examine core temperature while swimming a long distance (1000 m) at a fast pace in warm water (25.5 °C) while wearing a wetsuit. METHODS: Two experienced triathletes (mean ± standard deviation (SD), age 38.5 ± 23.3 years, height 1.83 ± 0.03 m, weight 80.1 ± 1.1 kg) participated in the study. At least 8 hours prior to attending the test session, participants swallowed an ingestible core temperature pill (BodyCap). Before beginning data collection, core temperature (Tc) data were transferred to a computer and sample rate was set to 0.1 Hz (1 sample every 10 s). Testing consisted of a self-directed warm up of 250-m followed by a 1000-m swim in an indoor pool (set to 25-m length) for each condition: no wetsuit (NW) and full sleeve wetsuit (FS). Participants swam at a self-selected pace at a “race pace” intensity (Borg Rating of Perceived Exertion = 16). Participants were required to rest until core temperature was within 0.5 °C of baseline before starting the next condition. Core temperature data were transferred to a computer after each swim. Average Tc of each swim was computed for analysis. RESULTS: The average core temperatures of NW and FS were 37.75 ± 0.11°C and 37.74 ± 0.46°C, respectively. The total swim time for the NW and FS conditions were 14:04 ± 1:43 and 13:05 ± 1:06 minutes. Mean stroke rate calculated for NW and FS were 30.12 ± 0.17 and 30.68 ± 0.45 strokes per minutes. Average swim speeds for NW and FS were 1.19 ± 0.15 and 1.28 ± 0.11 meters per second. CONCLUSIONS: Based upon the analysis of the results, the use of a wetsuit while swimming in warm water does not influence core temperature.

5 km front crawl in pool and open water swimming: breath-by-breath energy expenditure and kinematic analysis

Breath-by-breath energy expenditure during open water swimming has not yet been explored in an ecological environment. This study aimed to investigate and compare energetics and kinematics of 5km swimming, in both swimming pool and open water conditions. Through four independent studies, oxygen uptake ([Formula: see text]2) kinetics, heart rate (HR), blood lactate concentration ([La-]) and glucose level (BGL), metabolic power ([Formula: see text]), energy cost (C) and kinematics were assessed during 5km front crawl trials in a swimming pool and open water conditions. A total of 38 competitive open water swimmers aged 16-27years volunteered for this four part investigation: Study A (pool, ten females, 11 males), Study B (pool, four females, six males), Study C (pool case study, one female) and Study D (open water, three females, four males). In the swimming pool, swimmers started with an above average swimming speed (v), losing efficiency along the 5km, despite apparent homeostasis for [La-], BGL, [Formula: see text]2, [Formula: see text] and C. In open water, swimmers started the 5km with a below average v, increasing the stroke rate (SR) in the last 1000m. In open water, [Formula: see text]2 kinetics parameters, HR, [La-], BGL, respiratory exchange ratio and C were affected by the v and SR fluctuations along the 5km. Small fluctuations were observed for energetic variables in both conditions, but changes in C were lower in swimming pool than in open water. Coaches should adjust the training plan accordingly to the specificity of open water swimming.

Fibrous Flagellar Hairs of Chlamydomonas reinhardtii Do Not Enhance Swimming

The flagella of Chlamydomonas reinhardtii possess fibrous ultrastructures of a nanometer-scale thickness known as mastigonemes. These structures have been widely hypothesized to enhance flagellar thrust; however, detailed hydrodynamic analysis supporting this claim is lacking. In this study, we present a comprehensive investigation into the hydrodynamic effects of mastigonemes using a genetically modified mutant lacking the fibrous structures. Through high-speed observations of freely swimming cells, we found the average and maximum swimming speeds to be unaffected by the presence of mastigonemes. In addition to swimming speeds, no significant difference was found for flagellar gait kinematics. After our observations of swimming kinematics, we present direct measurements of the hydrodynamic forces generated by flagella with and without mastigonemes. These measurements were conducted using optical tweezers, which enabled high temporal and spatial resolution of hydrodynamic forces. Through our measurements, we found no significant difference in propulsive flows due to the presence of mastigonemes. Direct comparison between measurements and fluid mechanical modeling revealed that swimming hydrodynamics were accurately captured without including mastigonemes on the modeled swimmer’s flagella. Therefore, mastigonemes do not appear to increase the flagella’s effective area while swimming, as previously thought. Our results refute the longstanding claim that mastigonemes enhance flagellar thrust in C. reinhardtii, and so, their function still remains enigmatic.

Biologically inspired swimming robotic frog based on pneumatic soft actuators

Research on soft robots and swimming robots has been widely reported and demonstrated. However, none of these soft swimming robots can swim flexibly and efficiently using legs, just like a frog. This paper demonstrates a self-contained, untethered swimming robotic frog actuated by 12 pneumatic soft actuators, which can swim in the water for dozens of minutes by mimicking the paddling gait of the natural frog. We designed two kinds of pneumatic soft actuators as the joints on the robotic frog’s legs, which allows the legs to be lighter and more compact. It is found that such soft actuators have great potential in developing amphibious bionic robots, because they are fast-responding, inherently watertight and simple in structural design. The kinematic analysis in swimming locomotion was conducted for the prototype robotic frog, and the locomotion trajectory of each leg was planned based on the analysis of the paddling gait of frogs. Combined with the deformation model of the soft actuators, the robotic frog’s legs are controlled by coordinating the air pressure of each joint actuator. The robotic frog’s body is compact and the total mass is 1.29 kg. Different paddling gaits were tested to investigate swimming performance. The results show that the robotic frog has agile swimming ability and high environmental adaptability. The robotic frog can swim forward more than 0.6 m (3.4 times the body length) in one paddling gait cycle(6 s), whose average swimming velocity is about 0.1 m s−1, and the minimum turning radius is about 0.15 m (less than 1 body length).

Modeling and simulation of the intermittent swimming gait with the muscle-contraction model of pre-strains

The muscle-contraction model of pre-strains is employed to study the intermittent swimming gait of a 2D freely swimming fish. The fluid-structure interaction problem of fish swimming is solved with the finite elements method. Benefits of the intermittent swimming and the effects of the duty cycle and the tail swing number in a burst phase are emphasized. The results show that, compared to the steady swimming, a huge energy saving for swimming can be achieved by sacrificing a little speed during the intermittent swimming. Moreover, the fish swimming can be gradually transited from an intermittent mode to the continuous mode by increasing the duty cycle. With a smaller duty cycle, the fish can save more energy to obtain the same swimming velocity. A smaller tail swing number in a burst phase is more helpful to improve the energy utilization rate under the condition of Rduty = 0.5. In addition, the intermittent swimming will have more benefits at the lower and the higher average swimming speeds, and it is very important to have a proper proportion of the velocity bounds for the performance improvement of the intermittent swimming. These conclusions may have direct meanings for the development of biomimetic autonomous underwater vehicles.

Naringin provides neuroprotection in CCL2-induced cognition impairment by attenuating neuronal apoptosis in the hippocampus

BackgroundChemokine C–C motif ligand 2 (CCL2) is one of the most widely recognised proinflammatory chemokines in cognitive disorders. Currently, CCL2-targeting drugs are extremely limited. Thus, this study aimed to explore the neuroprotection afforded by naringin in CCL2-induced cognitive impairment in rats.MethodsBefore the CCL2 intra-hippocampal injection, rats were treated with naringin for 3 consecutive days via intraperitoneal injection. Two days post-surgery, the Morris water maze (MWM) and novel object recognition (NORT) tests were performed to detect spatial learning and memory and object cognition, respectively. Nissl staining and dUTP nick-end labelling (TUNEL) staining were performed to assess histopathological changes in the hippocampus. Commercial kits were used to measure the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) and the content of malondialdehyde (MDA). Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to examine the relative mRNA expression of interleukin 1β, (IL-1β), interleukin 6 (IL-6), glutamate/aspartate transporter (GLAST), glutamate transporter-1 (GLT-1), phosphate-activated glutaminase (PAG), cysteine aspartic acid-specific protease 8 (caspase-8), cysteine aspartic acid-specific protease 3 (caspase-3), cell lymphoma/leukaemia-2 (Bcl-2), and Bcl-2 associated X protein (Bax).ResultsIn the MWM, the average escape latency and average swimming distance were significantly reduced and the crossing times were increased in the naringin-treated groups, compared with the CCL2 group. The NORT results revealed that, compared with the CCL2 rats, the discrimination index in the naringin-treated rats increased significantly. Nissl and TUNEL staining revealed that naringin protected the structure and survival of the neurons in the CA1 zone of the hippocampus. In the naringin-treated groups, the SOD and GSH-Px activities were increased, whereas the MDA levels were decreased. Furthermore, in the naringin-treated groups, the relative mRNA expression of IL-1β and IL-6 was significantly decreased; GLAST and GLT-1 mRNA expression levels were increased, whereas PAG was decreased. In the naringin-treated groups, the relative mRNA expression levels of caspase-8, caspase-3, and Bax were decreased, whereas that of Bcl-2 was increased.ConclusionCollectively, these data indicated that naringin alleviated the CCL2-induced cognitive impairment. The underlying mechanisms could be associated with the inhibition of neuroinflammation, oxidative stress, apoptosis, and the regulation of glutamate metabolism.

Neuromodulatory Selection of Motor Neuron Recruitment Patterns in a Visuomotor Behavior Increases Speed.

Animals generate locomotion at different speeds to suit their behavioral needs. Spinal circuits generate locomotion at these varying speeds by sequential activation of different spinal interneurons and motor neurons. Larval zebrafish can generate slow swims for prey capture and exploration by activation of secondary motor neurons and much faster and vigorous swims during escape and struggle via additional activation of primary motor neurons. Neuromodulators are known to alter the motor output of spinal circuits, but their precise role in speed regulation is not wellunderstood. Here, in the context of optomotor response (OMR), an innate evoked locomotor behavior, we show that dopamine (DA) provides an additional layer to regulation of swim speed in larval zebrafish. Activation of D1-like receptors increases swim speed during OMR in free-swimming larvae. By analyzing tail bend kinematics in head-restrained larvae, we show that the increase in speed is actuated by larger tail bends. Whole-cell patch-clamp recordings from motor neurons reveal that, during OMR, typically only secondary motor neurons are active, whereas primary motor neurons are quiescent. Activation of D1-like receptors increases intrinsic excitability and excitatory synaptic drive in primary and secondary motor neurons. These actions result in greater recruitment of motor neurons during OMR. Our findings provide an example of neuromodulatory reconfiguration of spinal motor neuron speed modules where members are selectively recruited and motor drive is increased to effect changes in locomotor speed. VIDEO ABSTRACT.

Cephalopod-inspired robot capable of cyclic jet propulsion through shape change

The compliance and conformability of soft robots provide inherent advantages when working around delicate objects or in unstructured environments. However, rapid locomotion in soft robotics is challenging due to the slow propagation of motion in compliant structures, particularly underwater. Cephalopods overcome this challenge using jet propulsion and the added mass effect to achieve rapid, efficient propulsion underwater without a skeleton. Taking inspiration from cephalopods, here we present an underwater robot with a compliant body that can achieve repeatable jet propulsion by changing its internal volume and cross-sectional area to take advantage of jet propulsion as well as the added mass effect. The robot achieves a maximum average thrust of 0.19 N and maximum average and peak swimming speeds of 18.4 cm s−1 (0.54 body lengths/s) and 32.1 cm s−1 (0.94 BL/s), respectively. We also demonstrate the use of an onboard camera as a sensor for ocean discovery and environmental monitoring applications.

One size does not fit all: inter- and intraspecific variation in the swimming performance of contrasting freshwater fish.

Artificial barriers cause widespread impacts on freshwater fish. Swimming performance is often used as the key metric in assessing fishes' responses to river barriers. However, barrier mitigation is generally based on the swimming ability of salmonids and other strong swimmers because knowledge of swimming ability for most other freshwater fish is poor. Also, fish pass designs tend to adopt a 'one size fits all' approach because little is known about population or individual variability in swimming performance. Here, we assessed interspecific and intraspecific differences in the sustained swimming speed (Usus ) of five freshwater fish with contrasting body sizes, morphologies and swimming modes: topmouth gudgeon, European minnow, stone loach, bullhead and brown trout. Significant U sus variation was identified at three organizational levels: species, populations and individual. Interspecific differences in Usus were as large as 64cms-1, upstream populations of brown trout showed mean U sus 27cms-1 higher than downstream populations, and species exhibited high individual variation (e.g. cv = 62% in European minnow). Sustained swimming speed (U sus) increased significantly with body size in topmouth gudgeon, European minnow and brown trout, but not in the two benthic species, bullhead and stone loach. Aerobic scope had a significant positive effect on U sus in European minnow, stone loach and brown trout. Sustained swimming speed (U sus) decreased with relative pectoral fin length in European minnow and brown trout, whereas body fineness was the best predictor in stone loach and bullhead. Hence, swimming performance correlated with a diverse range of traits that are rarely considered when predicting fish passage. Our study highlights the dangers of using species' average swimming speeds and illustrates why a 'one size fits all' approach often fails to mitigate for barrier effects. We call for an evidence-based approach to barrier mitigation, one that recognizes natural variability at multiple hierarchical levels.

Kinematic, Coordinative and Efficiency Parameters of Physically Impaired Swimmers at Maximum Aerobic Power Speed

Background:In paralympic swimming, the biomechanical parameters related to performance are effectively determined according to the potentialities and peculiarities of each athlete. However, a clear integrated approach to these parameters for swimmers with physical disabilities at the speed of maximum oxygen uptake (vV̇O2max) is still practically non-existent.Objective:The purpose of this study was twofold: (i) to assess kinematic, coordinative and efficiency parameters measured at vV̇O2maxin swimmers with physical impairments; and (ii) to correlate these biomechanical parameters with the time for a 200 m maximum test.Methods:Eleven swimmers with physical disabilities (seven males and four females) were assessed at vV̇O2maxwith support from a three-dimensional kinematic method. The performance parameters analysed were: (i) kinematic - stroke rate (SR), stroke length (SL), average swimming speed (SS) and intra-cyclic velocity variation (IVV); (ii) coordinative - index of coordination (IdC) and adapted index of coordination (IdCadapt); and (iii) swimming efficiency - propelling efficiency (çp).Results:The overall results showed high dispersion and wide confidence intervals for the kinematic and coordinative variables. The mean and standard deviation of vV̇O2maxand V̇O2at the same intensity were 0.90 ± 0.13 m/s and 38.2 ± 8.3 ml/kg/min, respectively.Conclusion:Swimmers with less significant impact of physical disability on specific swimming tasks presented higher SL, SS and çp. The IVV was higher in swimmers with a greater impact of disability on conducting specific competitive swimming tasks. In general, the catch-up inter-arm coordination model is adopted.

Physicochemical characterization of Suvarna Bhasma, its toxicity profiling in rat and behavioural assessment in zebrafish model.

Suvarna Bhasma is a gold-based Ayurved medicine that has a wide range of therapeutic indications like tuberculosis, diabetes mellitus, rheumatoid arthritis and nervous diseases. Suvarna Bhasma is also used in Suvarnaprashana, an Ayurved advocated therapy being practised to improve immunity in children. To augment traditional understanding, here we present an evidence-based study on Suvarna Bhasma regarding its physicochemical properties, toxicity and efficacy. Suvarna Bhasma was characterised by physicochemical characterization techniques such as scanning electron microscope (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD) and atomic emission spectroscopy (ICP-AES). Toxicity of Suvarna Bhasma was studied in Holtzman rats with daily oral dose from 3 mg/kg (therapeutic dose, TD) up to 30 mg/kg (10 TD) body weight for 90 days. Behavioural study, such as motor and geotactic behaviour were examined in zebrafish model to find out any sign of neurotoxicity or behavioural changes due to Suvarna Bhasma administration. Suvarna Bhasma has two types of gold particles, large ones (~60 μm) having irregular shapes, and nano-sized spherical particles (starting from ~10 nm), the latter coated with Fe, Si, O, P and Na. XRD study revealed that all the peaks of Suvarna Bhasma match well with pure gold (face centred cube) with crystallites size 45 ± 2.8 nm. In rat studies, some change in biochemical parameters such as urea, creatinine and alanine aminotransferase (ALT) was observed mainly at the higher therapeutic dose; however, those parameters were within the normal range. There were no significant macroscopic as well as microscopic treatment-related alteration observed, in any of the organs and tissues evaluated. In zebrafish behavioural study, the motor parameters of Suvarna Bhasma treated fish showed normal behaviour analogous to the vehicle control group. Interestingly, the geotactic behaviour showed anxiolytic effects of Suvarna Bhasma as evidenced by the time spent in the upper zone, and average swimming height. The anxiolytic effects persisted for more than 30 days after withdrawing the Suvarna Bhasma treatment. Suvarna Bhasma contained spherical gold nanoparticles. It was nontoxic in rat model at the does tested. Suvarna Bhasma has anxiolytic effects in zebrafish behavioural model.

Abnormal brain activity in rats with sustained hypobaric hypoxia exposure: a resting-state functional magnetic resonance imaging study.

Background:Hypobaric hypoxia (HH) exposure at high altitudes can result in a decline in cognitive function, which may have a serious impact on the daily life of people who migrate to high altitudes. However, the specific HH-induced changes in brain function remain unclear. This study explored changes in brain activity in rats exposed to a sustained HH environment using functional magnetic resonance imaging (fMRI).Methods:Healthy male rats (8 weeks old) were randomly divided into a model group and a control group. A rat model of cognitive impairment induced by sustained HH exposure was established. The control and model groups completed training and testing in the Morris water maze (MWM). A two-sample t-test for between-group difference comparisons was performed. Repeated measures analyses of variance for within-group comparisons were performed and post-hoc comparisons were made using the Tukey test. Between-group differences in spontaneous brain activity were assessed using a voxel-wise analysis of resting-state fMRI (rs-fMRI), combined with analyses of the fractional amplitude of low frequency fluctuations (fALFF) in statistical parametric mapping.Results:In the MWM test, the escape latencies of the model group were significantly longer compared with those of the control group (control group vs. model group, day 1: 21.6 ± 3.3 s vs. 40.5 ± 3.4 s, t = –11.282; day 2: 13.5 ± 2.2 s vs. 28.7 ± 5.3 s, t = –7.492; day 3: 10.5 ± 2.8 s vs. 22.6 ± 6.1 s, t = –5.099; day 4: 9.7 ± 2.5 s vs. 18.6 ± 5.2 s, t = –4.363; day 5: 8.8 ± 2.7 s vs. 16.7 ± 5.0 s, t = –3.932; all P < 0.001). Within both groups, the escape latency at day 5 was significantly shorter than those at other time points (control group: F = 57.317, P < 0.001; model group: F = 50.718, P < 0.001). There was no within-group difference in average swimming speed (control group, F = 1.162, P = 0.956; model group, F = 0.091, P = 0.880). Within the model group, the time spent within the original platform quadrant was significantly shorter (control group vs. model group: 36.1 ± 5.7 s vs. 17.8 ± 4.3 s, t = 7.249, P < 0.001) and the frequency of crossing the original platform quadrant was significantly reduced (control group vs. model group: 6.4 ± 1.9 s vs. 2.0 ± 0.8 s, t = 6.037, P < 0.001) compared with the control group. In the rs-fMRI study, compared with the control group, rats in the model group showed widespread reductions in fALFF values throughout the brain.Conclusions:The abnormalities in spontaneous brain activity indicated by the fALFF measurements may reflect changes in brain function after HH exposure. This widespread abnormal brain activity may help to explain and to provide new insights into the mechanism underlying the impairment of brain function under sustained exposure to high altitudes.

Bite Out Your Tongue

Bite Out Your Tongue

Swimming Performance of Rainbow Trout and Westslope Cutthroat Trout in an Open-Channel Flume

AbstractWe used an open-channel flume to characterize the swimming performance of Rainbow Trout Oncorhynchus mykiss and Westslope Cutthroat Trout Oncorhynchus clarki lewisi ranging nominally in fork length from 15 to 30 cm. With an open-channel flume, we observed volitional swim performance of wild-caught Rainbow Trout and Westslope Cutthroat Trout; the fish were not coerced, prodded, or spooked into action. We also observed the maximum short-duration swim speed of the fish, providing important effective leap or velocity challenge information for the design of intentional barriers. We conducted the experiment with a consistently low water velocity challenge and characterized swim speeds by using weighted least-squares regression, revealing no evidence of a difference in swim speeds between the two species. We estimated the overall average swim speed for Rainbow Trout to be 0.84 m/s (SE = 0.02), with a 95% confidence interval of 0.79–0.89 m/s, and that for Westslope Cutthroat Trout to be 0.84 m/s (SE = 0.03), with a 95% confidence interval of 0.78–0.90 m/s. The maximum swim speeds observed were 2.72 m/s for Rainbow Trout and 3.55 m/s for Westslope Cutthroat Trout. The project results provide new information on the swimming ability of wild Rainbow Trout and Westslope Cutthroat Trout that can be used to improve fish passage or barrier design.