This paper presents the results obtained from the analysis of physiological and biochemical parameters in the radish, species Raphanus sativus L., variety Rond Ecarlate, which was grown in three different systems (in the field, in the solarium and in the climate chamber). Several parameters necessary for the evaluation of biomass, physiological processes and nutritional values were determined. Regarding the biometric parameters, it was noticed that in the field cultivation the plants formed 11-12 leaves, in the solarium average 12-13 leaves, and in the climatic chamber the analyzed plants formed 7-9 leaves, on an average. The total biomass of the plant was recorded with the following average values: in the field 70.05 grams, in the solarium 92.3 grams and in the climate chamber 32.9 grams. Regarding the circumference of the tuberous hypocotyl axis, the values are higher in plants grown in solarium (16.9 cm), with the smallest dimensions being recorded in plants grown in the climate chamber (7.2 cm). The highest average pulp firmness value was observed in plants grown in the solarium (0.27 kg force), and the lowest values were seen in plants grown in the climate chamber (0.14 kg force). Regarding the amount of water lost through perspiration by the leaves of radish plants, the highest average values were recorded at plants grown in the solarium, and the lowest at plants grown in the climatic chamber. The average soluble dry matter content was higher (over 4%) in plants grown in the climate chamber compared to other harvesting systems. The analysis of the average carbohydrate content shows that the total soluble carbohydrates were present only in the hypocotyl axis and glucose was found both in the hypocotyl axis of the studied plants and in the leaves. Vitamin C was present in variable amounts, both in the hypocotyl axis and in the leaves. The average amino acid content (expressed in amino nitrogen) was between 0.003% and 0.006%. The anthocyanin was present with highest values (54.5 mg) in field-grown plants, and catalase activity was present both in the hypocotyl axis and in the leaves and roots. Air humidity and temperature had a high influence on the development of plant biomass, on the content of dry matter and on the loss of water through transpiration. The biometric, physiological and biochemical parameters varied depending on the cultivation system.