When the reactor is added with ectoine of concentrations of 0, 0.1, 1 and 10 mmol/L, the impacts on brine waste treatment efficiency are investigated. The results show that the outflow COD and ammonia nitrogen removal rates are the highest, when the ectoine concentration is 0.1 mmol/L. The brine waste treatment efficiency under addition of ectoine of 1 and 10 mmol/L is even worse than that without ectoine addition. It can be preliminarily determined that the best ectoine dosage is in between 0.1-1.0 mmol/L. When ectoine concentrations added in reactors are 0.2, 0.5, 0.8 and 1.0 mmol/L, the results show that the average reactor outflow COD and ammonia nitrogen removal rates are increased compared with those of reactor without adding ectoine. But when ectoine of 1.0 mmol/L is added, the outflow COD and ammonia nitrogen removal rates decrease. When ectoine dosage is 0.5 mmol/L, the reactor outflow COD and ammonia nitrogen values are the lowest, the removal rates are the highest, the average COD removal rate is 74.46%, and the average ammonium nitrogen removal rate is 54.97%. Compared with reactor without adding ectoine, COD and ammonium nitrogen removal rates are increased by 13.16% and 26.81%. Therefore, the best dosage of ectoine is 0.5 mmol/L.