This research paper is part of a wider project in which the influence of various factors on the performance of the company is analyzed. The impact of the gender structure of general legal representatives as well as the form of organization of the company on profitability expressed through the average gross profit margin was studied. The five-year period from 2018 to 2022 was studied, in which data from the profit and loss statements of a total of 192 entities were analyzed. The sample presents six groups classified by the form of organization of companies that can be encountered in business practice. All statistical analyses were carried out in the IBM SPSS 23 program. In the research, the hypotheses about the statistically significant influence of the difference in the gender of general representatives on the profitability of the company were rejected, and the hypothesis about the significant differences in the influence of the form of organization of the company on the profitability expressed by the average gross profit margin, which was accepted. Research into factors that can affect the profitability of the analyzed subjects should enable the making of better management decisions in order to realize better and better performance of the company.
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