To explore the dynamic evolution of ecosystem services in the last 22 years, and to measure the trade-off and synergy between ecosystem services, it is of great significance to realize the ecological protection and sustainable development of the Northeast Tiger and Leopard National Park (NTLNP). Therefore, based on land use changes, soil and meteorological data, this research analyzed the changes of land use in NTLNP from 2000 to 2022. The habitat quality (HQ), carbon storage (CS), water conservation (WC) and soil conservation (SC) in the NTLNP were evaluated by the InVEST model, and the synergy/trade-off among different ecosystem services were analyzed quantitatively. The results are as follows: (1) Land use in NTLNP changed from broad-leaved forest to mixed and coniferous forest from 2000 to 2022. (2) Average HQ increased over time, while CS increased in coniferous and mixed forest areas, but decreased in cropland and grassland areas. WC decreased in the central and southern regions. SC decreased in coniferous forests in the southern part of the study area. (3) HQ showed a trade-off relationship with CS, WC, and SC of 50%, mainly distributed in the southwest of NTLNP at higher elevations. CS showed synergistic correlations with WC and SC, mainly distributed in grassland and broad-leaved forest areas in the southwest; areas showing trade-off relationships are mainly distributed in cropland and broad-leaved forest areas. WC and SC showed a synergistic correlation in 90.2% of the areas. In the past 22 years, ecosystem service capacity of NTLNP is increasing; in the future, we should pay more attention to the optimization of NTLNP function zoning, and gradually explore and improve the national park management system, to balance the relationship between the protection of ecological resources and the development of human society and economy.
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