Maize plays a crucial role in China’s grain production, with a cultivation area reaching 44.22 million hectares and an annual yield of 289 million tons in 2023. However, the challenge remains on how to further increase maize yield and water use efficiency (WUE) without adding to the environmental burden. To systematically evaluate the impact of straw incorporation under varying production conditions on maize yield and WUE, this study collected experimental data from multiple locations across China. A meta-analysis was conducted to compare the effects of straw incorporation versus no incorporation, and the main influencing factors were identified using correlation analysis and a random forest model. The results indicate that straw incorporation significantly enhances both maize yield and WUE, with the most pronounced improvements observed under conditions of an average growing season temperature of 19–23 °C, soil pH of 6.5–7.5, low initial soil organic matter content, and deep plowing for straw incorporation. Additionally, moderate nitrogen application rates and straw incorporation amounts (9000–15,000 kg·ha−2) also significantly boost maize yield and WUE. Field management practices and meteorological conditions are identified as the primary factors affecting maize yield and WUE under straw incorporation conditions. Therefore, straw incorporation stands out as an effective agricultural practice for achieving high maize yields and efficient resource utilization. The findings of this study provide valuable insights for global food security and the sustainable development of agriculture.