Maize grains are deficient in sulfur-containing amino acids such as methionine (Met) and cysteine (Cys). To date, no maize hybrid rich in Met and Cys has been commercialized particulalry in the subtropical regions. In this study, molecular characterization among seven inbreds using 89 SSRs grouped them into three clusters. The average genetic dissimilarity (GD) coefficient and gene diversity among these inbreds were 0.708 and 0.478, respectively. These seven inbreds were crossed in 7 × 7 half-diallel mating design, and the resulting 21 F1 hybrids along with four popular hybrid checks were evaluated across three locations viz., Delhi, Vagarai and Gauria Karma. Genetic analysis revealed that additive gene action predominantly controls Met content of f, while, both additive and non-additive gene action are important for Cys. Met and Cys levels varied from 0.058-0.306% and 0.025-0.126% with mean of 0.196% and 0.060%, respectively. Promising hybrids identified include MGU-MH-20 (Met: 0.306%, Cys: 0.040%, grain yield: 8136kgha-1), MGU-MH-17 (Met: 0.269% Cys: 0.045%, grain yield: 8178kgha-1), MGU-MH-19 (Met: 0.254%, Cys: 0.048%, grain yield: 8588kgha-1), and MGU-MH-18 (Met: 0.253%, Cys: 0.034%, grain yield: 9812kgha-1) were identified. No correlation was observed among Met, Cys, grain yield and GD. This study is the first to examine the genetics of Met and Cys accumulation in maize. Our findings provide valuable insights for the development of high Met and Cys maize cultivars, which are vital to mitigate malnutrition, especially in developing countries.
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