Avena Sterilis Research Articles

First report of Ophiosphaerella narmari causing spring dead spot of hybrid bermudagrass in China.

Hybrid bermudagrass (Cynodon dactylon×C. transvaalensis) is widely used as turf in transition zone of China. Spring dead spot (SDS) is one of the most damaging diseases of hybrid bermudagrass. Symptoms of SDS appear when hybrid bermudagrass starts to break dormancy with warm temperature in early spring. The symptoms show sunken, circular or irregularly shaped, straw-colored patches, with 20 to 100 cm in diameter. The patches maintain dormant as the surrounding uninfected turfgrass resumes growth and turns green. SDS pathogens are soilborne fungi that colonize roots, stolons and rhizomes, infected roots or rhizomes become black and eventually collapse. Three species of fungi are reported to cause SDS: Ophiosphaerella herpotricha (Fr) J. Walker; O. korrae (J. Walker & A.M. Smith) Shoemaker & C.E. Babcock; or O. narmari (J. Walker & A.M. Smith) Wetzel, Hubert & Tisserat (Walker and Smith 1972; Walker 1980; Shoemaker and Babcock 1989; Wetzel et al. 1999). However, distribution of the three species may vary by geographical region (Cottrill et al. 2016). In October 2020, symptoms of SDS were observed on hybrid bermudagrass fairways of Taihu golf course in Wuxi, Jiangsu province. Root samples of SDS were collected, symptomatic roots with 3-4 cm length were cut, washed 2-3 times, surface sterilized in 0.6% NaOCl for 5 min, rinsed and blotted dry for 2 min and placed on potato dextrose agar (PDA) amended with 50 mg L-1 each of ampicillin, streptomycin sulfate and tetracycline. Plates were incubated in the dark at 25℃ for 5-7 days, Hyphae growing from the roots were transferred to new PDA plates. A total of 7 fungal isolates with morphology similar to SDS pathogens were obtained (Tredway et al. 2009). The genomic DNA was extracted from 2 of them (7-41, 8-6) and amplified with universal primers ITS5 and ITS4 (White et al. 1990). PCR products were sequenced (deposited as MW536995 and MW536994 in GenBank, not available yet) and showed 99.79% similarity to O. narmari (KP690979). Pathogenicity tests were performed on 'Tifdwarf' hybrid bermudagrass (9-week-old in 5 × 20 cm Cone-Tainers containing a sand and nutrition substrate mixture). Eight oat seeds infested with O. narmari were inserted 5 cm below the soil surface in the root zone of hybrid bermudagrass. The inoculated turfgrass grew for five weeks in the growth chamber with a 12-h day/night cycle of 25/20°C and 90% relative humidity. A control treatment was inoculated with 8 noninfested sterile oat seeds, and each treatment was replicated 3 times. The root tissues of hybrid burmudagrass inoculated with O. narmari became black and necrotic, no symptoms were observed on the roots of noninfested plants. O. narmari was consistently reisolated from symptomatic roots, and confirmed by PCR as mentioned above. To the best of our knowledge, this is the first report of O. narmari caused spring dead spot in the transition zone of China. The identification of SDS caused by O. narmari will have important implications for the management of this root-rot species on hybrid bermudagrass.

Differences in Germination of ACCase-Resistant Biotypes Containing Isoleucine-1781-Leucine Mutation and Susceptible Biotypes of Wild Oat (Avena sterilis ssp. ludoviciana).

Herbicide resistance can affect seed germination and the optimal conditions required for seed germination, which in turn may impose a fitness cost in resistant populations. Winter wild oat [Avena sterilis L. ssp. ludoviciana (Durieu) Gillet and Magne] is a serious weed in cereal fields. In this study, the molecular basis of resistance to an ACCase herbicide, clodinafop-propargyl, in four A. ludoviciana biotypes was assessed. Germination differences between susceptible (S) and ACCase-resistant biotypes (WR1, WR2, WR3, WR4) and the effect of Isoleucine-1781-Leucine mutation on germination were also investigated through germination models. The results indicated that WR1 and WR4 were very highly resistant (RI > 214.22) to clodinafop-propargyl-contained Isoleucine to Leucine amino acid substitution. However, Isoleucine-1781-Leucine mutation was not detected in other very highly resistant biotypes. Germination studies indicated that resistant biotypes (in particular WR1 and WR4) had higher base water potentials than the susceptible one. This shows that resistant biotypes need more soil water to initiate their germination. However, the hydrotime constant for germination was higher in resistant biotypes than in the susceptible one in most cases, showing faster germination in susceptible biotypes. ACCase-resistant biotypes containing the Isoleucine-1781-Leucine mutation had lower seed weight but used more seed reserve to produce seedlings. Hence, integrated management practices such as stale seedbed and implementing it at the right time could be used to take advantage of the differential soil water requirement and relatively late germination characteristics of ACCase-resistant biotypes.

Discovery and Chromosomal Location a Highly Effective Oat Crown Rust Resistance Gene Pc50-5.

Crown rust, caused by Puccinia coronata f. sp. avenae, is one of the most destructive fungal diseases of oat worldwide. Growing disease-resistant oat cultivars is the preferred method of preventing the spread of rust and potential epidemics. The object of the study was Pc50-5, a race-specific seedling crown rust resistant gene, highly effective at all growth stages, selected from the differential line Pc50 (Avena sterilis L. CW 486-1 × Pendek). A comparison of crown rust reaction as well as an allelism test showed the distinctiveness of Pc50-5, whereas the proportions of phenotypes in segregating populations derived from a cross with two crown rust-susceptible Polish oat cultivars, Kasztan × Pc50-5 and Bingo × Pc50-5, confirmed monogenic inheritance of the gene, indicating its usefulness in oat breeding programs. Effective gene introgression depends on reliable gene identification in the early stages of plant development; thus, the aim of the study was to develop molecular markers that are tightly linked to Pc50-5. Segregating populations of Kasztan × Pc50-5 were genotyped using DArTseq technology based on next-generation Illumina short-read sequencing. Markers associated with Pc50-5 were located on chromosome 6A of the current version of the oat reference genome (Avena sativa OT3098 v2, PepsiCo) in the region between 434,234,214 and 440,149,046 bp and subsequently converted to PCR-based SCAR (sequence-characterized amplified region) markers. Furthermore, 5426978_SCAR and 24031809_SCAR co-segregated with the Pc50-5 resistance allele and were mapped to the partial linkage group at 0.6 and 4.0 cM, respectively. The co-dominant 58163643_SCAR marker was the best diagnostic and it was located closest to Pc50-5 at 0.1 cM. The newly discovered, very strong monogenic crown rust resistance may be useful for oat improvement. DArTseq sequences converted into specific PCR markers will be a valuable tool for marker-assisted selection in breeding programs.

Competition of Weeds Dominated by Wild Oat (Avena sterilis L.) in Wheat (Triticum durum Desf.) in Jordan

Wheat is the main field crop grown in Jordan. Productivity is low due to different factors, including erratic rainfall, poor soil fertility and weed competition. A field experiment was carried out at the University of Jordan Research Station for two growing seasons to determine the effect of weed competition on growth and yield of wheat (Triticum durum Cv. “Hourani”). Treatments consisted of either allowing weeds to infest the crop or maintaining the crop weed-free for increasing durations after emergence. Results showed that, the longer the periods of weed competition, the greater the loss in crop growth and yield. Average reductions in grain and straw yields were 41% and 37%, respectively. The highest grain yield obtained was from weed-free, and the lowest was in weed-infested plots for the entire growing season. Average grain yield was not significantly different at 14 to 49 days of weed-infested periods, while none of the weed-free periods produced a yield similar to that of the weed-free control. However, maintaining a weed-free crop for three weeks after emergence significantly increased grain yield compared with the weed-infested control. High rainfall in the first season almost doubled weed growth and greatly reduced wheat growth and yield compared with the second season. To determine the critical period of weed competition and the influence of weed infestation on wheat grain yield, Gompertz and logistic equations were fitted to data representing increasing duration of weed-free and weed-infested periods, respectively. Based upon an arbitrary 5% level of average grain yield loss in the two years, the critical period of weed competition occurred at 0–49 days after wheat emergence, which corresponded with the rapid increase in weed biomass.

Repetitive hygroscopic snapping movements in awns of wild oats

Wild oat (Avena sterilis) is a very common annual plant species. Successful seed dispersion support its wide distribution in Africa, Asia and Europe. The seed dispersal units are made of two elongated stiff awns that are attached to a pointy compartment containing two seeds. The awns bend and twist with changes in humidity, pushing the seeds along and into the soil. The present work reveals the material structure of the awns, and models their functionality as two-link robotic arms. Based on nano-to-micro structure analyses the bending and twisting hygroscopic movements are explained. The coordinated movements of two sister awns attached to one dispersal unit were followed. Our work shows that sister awns intersect typically twice every wetting-drying cycle. Once the awns cross each other, epidermal silica hairs are suggested to lock subsequent movements, resulting in stress accumulation. Sudden release of the interlocked awns induces jumps of the dispersal unit and changes in its movement direction. Our findings propose a new role to epidermis silica hairs and a new facet of wild oat seed dispersion. Reversible jumping mechanism in multiple-awn seed dispersal units may serve as a blueprint for reversibly jumping robotic systems. Statement of significanceThe seed dispersal unit of wild oats carries two elongated stiff awns covered by unidirectional silica hairs. The awns bend and twist with changes in humidity, pushing the seed capsule along and into the ground. We studied structures constructing the movement mechanism and modeled the awn as a two-link robotic arm. We show that sister awns, attached to the same seed capsule, intersect twice every drying cycle. Once the awns cross each other, the epidermal silica hairs are suggested to lock any subsequent movements, causing stress accumulation. Sudden release of the interlocked awns may cause the dispersal unit to jump and change its direction. Our findings suggest a new role to silica hairs and a new dispersal mechanism in multiple-awn seed dispersal units.

Effect of different levels of urea and herbicide application on the yield of wheat (Triticum aestivum) in competition with Sinapis arvensis and Avena ludoviciana Durieu

Understanding the interaction between crop-weed competition and herbicide doses may, in many cases, result in a recommendation to reduce the consumption of the herbicide, which is both environmentally and economically important. Nitrogen fertilizer also influences the competitive balance through changes in weed density and biomass, and also contributes to environmental pollution. Hence, the evaluation of the interaction of herbicide doses and nitrogen fertilizer application is necessary for proper management of weeds and achieving acceptable economic yield along with minimizing environmental negative impacts.Therefore, in order to Quantify wheat-weed competition to levels of urea and reduced dose of herbicide (mesosulfuron methyl + iodosulfuron methyl sodium + diflufenican + mefenpyr-diethyl) brand (othello OD) this experiment was conducted during 2019–20 at Khuzestan University of Agricultural Sciences and Natural Resources, Iran. The experiment was laid out in split plots under randomized complete block design wherein urea fertilizer included five levels of 0, 45, 90, 180 and 330 kg/ha as the main plot and the herbicide dose of “othello OD” in six levels of 0, 0.32, 0.64, 0.96, 1.28 and 1.6 L/ha (1.6 L/ha the recommended dose) was considered as the sub plot. The results showed that wheat grain yield and weed biomass response to herbicide doses of sigmoid response and response dosage models, and developed in response to an increase in urea fertilizer consumption. Based on the prediction of the combined model, The achieving a yield of about 500 g/m2 with the application of 140 kg of urea fertilizer per hectare with 1.04 L/ha of Othello OD herbicide or the application of 265 kg of urea fertilizer per hectare and 0.64 L/ha. Hectare of herbicide or application of 330 kg urea fertilizer per hectare with 0.72 L/ha of herbicide was possible.

Nanoemulsion of Foeniculum vulgare essential oil: A propitious striver against weeds of Triticum aestivum

Essential oil based bioherbicides are topic of interest for the new era agriculture because of growing concern worldwide to develop herbicide with low environmental impact. In the present study, oil in water nanoemulsions of fennel (Foeniculum vulgare Mill.) essential oil were formulated by ultrasonic emulsification method and evaluated for herbicidal potential against Phalaris minor Retz., Avena ludoviciana Durieu, Rumex dentatus L. and Medicago denticulata Willd through Petri dish bioassay. The germination of P. minor, A. ludoviciana, R. dentatus and M. denticulata was totally inhibited at concentrations 0.4, 0.3, 0.3 and 0.05 wt%. Major constituents of F. vulgare essential oil, estragole, anethole and binary mixture did not completely inhibit the germination of the tested weed species even at highest concentrations. Nanoemulsions having 0.05 and 0.01 wt% F. vulgare essential oil were spherical in nature with average size below 130 nm and have good stability to centrifugation and dilution. The formulated nanoemulsions were clear and transparent even after 30 days of storage at ambient temperature. Nanoemulsions were more effective and completely inhibited the germination of P. minor, A. ludoviciana, R. dentatus and M. denticulata even at low dose of 0.05 wt% by adversely affecting the physiological processes like membrane leakage and reactive oxygen species mediated cellular damage. Nanoemulsions of F. vulgare essential oil have bioherbicidal properties and can contribute to the development of alternative tool for sustainable management of weeds.

Effect of drought stress on herbicide performance and photosynthetic activity of Avena sterilis subsp. ludoviciana (winter wild oat) and Hordeum spontaneum (wild barley)

AbstractThe severity of drought is increasing owing to global climate change. Knowledge about the influence of drought on weeds and herbicide performance is necessary for optimising herbicide applications. Therefore, dose‐response trials were conducted to investigate the influence of drought stress on the efficacy of four herbicides. Drought‐stressed and non‐stressed Hordeum spontaneum seedlings were exposed to nine doses of sulfosulfuron and sulfosulfuron + metsulfuron‐methyl. Clodinafop‐propargyl and mesosulfuron‐methyl + iodosulfuron‐methyl sodium were applied to drought‐stressed and non‐stressed seedlings of Avena sterilis subsp. ludoviciana. Leaf greenness index (SPAD), total chlorophyll content, stomatal conductance and photosynthesis rate of both species were measured. The efficacy of herbicides on H. spontaneum was not influenced by drought stress. In contrast, the performance of herbicides on A. sterilis subsp. ludoviciana was reduced under drought stress, where the ED50 increased by 1.5‐fold. The low doses (<2.2 g a.i. ha−1) of mesosulfuron‐methyl + iodosulfuron‐methyl‐sodium caused hormesis on A. sterilis subsp. ludoviciana. Total chlorophyll content and SPAD index of both species increased due to drought stress. The stomatal conductance and photosynthesis rate of A. sterilis subsp. ludoviciana decreased under drought stress, while H. spontaneum was not influenced. The lack of detrimental effect of drought stress on sulfosulfuron and sulfosulfuron + metsulfuron‐methyl performance might be attributed to similar stomatal conductance and photosynthesis rate of the drought‐stressed and non‐stressed H. spontaneum. The performance reduction in herbicides on drought‐stressed A. sterilis subsp. ludoviciana might be owing to the reduction in stomatal conductance and photosynthesis rate. Using adjuvants, diverse formulations and early application of herbicides were suggested for future research to hamper the negative effect of drought on herbicides.

Interference of wild oat (Avena fatua) and sterile oat (Avena sterilis ssp. ludoviciana) in wheat

Abstract Prevalence of wild oat (Avena fatua L.) and sterile oat [Avena sterilis ssp. ludoviciana (Durieu) Gillet & Magne; referred to as A. sterilis hereafter], winter-season weeds, is increasing in the eastern grain region of Australia. Biological attributes of these weeds enable them to survive in a wide range of environments and under different weed infestation levels. The interference of A. fatua and A. sterilis in a wheat (Triticum aestivum L.) crop was examined in southeast Queensland, Australia, through field studies in 2019 and 2020. Different infestation levels (0, 3, 6, 12, 24, and 48 plants m−2) of A. fatua and A. sterilis were evaluated for their potential to cause yield losses in wheat. Based on a three-parameter logarithmic model, the A. fatua and A. sterilis infestation levels corresponding to 50% wheat yield loss were 15 and 16 plants m−2, respectively. The yield reduction was due to a reduced spike number per unit area because of an increased weed infestation level. At the highest weed infestation level (48 plants m−2), A. fatua and A. sterilis produced a maximum of 4,800 and 3,970 seeds m−2, respectively. Avena fatua exhibited lower seed retention (17% to 39%) than A. sterilis (64% to 80%) at wheat harvest, as most of the seeds of A. fatua had shattered at crop maturity. Our results implied that there is a good opportunity for harvest weed seed control if the paddock is infested with A. sterilis. This study suggests that in the absence of an integrated weed management strategy (using both chemical and nonchemical options), a high infestation of these weeds could cause a severe crop yield loss, increase weed seed production, and replenish the weed seedbank in the soil.

Novel sensor-based method (quick test) for the in-season rapid evaluation of herbicide efficacy under real field conditions in durum wheat

AbstractOptimum herbicide use is a key factor affecting the success of any integrated weed management strategy. The main objective of the current study was to implement a method based on spectrometer measurements for the in situ evaluation of herbicide efficacy and the detection of potentially herbicide-resistant weeds. Field trials were conducted in Greece between 2018 and 2020 in several durum wheat fields (Triticum durum Desf.). In all trials, the overall effect of herbicide application on the recorded Normalized Difference Vegetation Index (NDVI) values (at 1 and 2 wk after treatment [WAT]) was significant (P ≤ 0.001). For the majority of the surveyed fields, low NDVI values were recorded after 2,4-D application and a mixture of clopyralid + florasulam from 1 WAT, suggesting their increased efficacy. In several cases, the application of pyroxsulam + florasulam resulted in significant NDVI reductions at 2 WAT. As observed at the end of the growing seasons, the herbicides that reduced NDVI resulted in lower weed biomass. Strong correlations were observed between weed aboveground biomass and NDVI (2 WAT). In particular, R2 values were 0.8234 to 0.8649, 0.8453, 0.8595, 0.8149, and 0.8925 for the Aliartos, Thiva, Domokos, Larissa, and Orestiada fields, respectively. The overall effects of herbicide application on wheat grain yield were also significant (P ≤ 0.001). Pot experiments confirmed that the high NDVI values in some cases could be attributed to the presence of herbicide-resistant weeds. For instance, the resistance indices of two accessions of catchweed bedstraw (Galium aparine L.) to mesosulfuron-methyl + iodosulfuron-methyl-sodium ranged between 9.7 and 13.2, whereas one sterile oat [Avena sterilis L. ssp. ludoviciana (Durieu) Gillet & Magne] accession was 8.8 times more resistant to fenoxaprop-p-ethyl than a susceptible one. The present study is targeted at making a significant contribution toward establishing cause–effect relationships and presenting a useful tool for developing more effective weed management practices in more arable crops and under different soil and climatic conditions.

Deciphering the dynamic gene expression patterns of pollen abortion in a male sterile line of Avena sativa through transcriptome analysis at different developmental stages

BackgroundMale sterility (MS) has important applications in hybrid seed production, and the abortion of anthers has been observed in many plant species. While most studies have focused on the genetic factors affecting male sterility, the dynamic gene expression patterns of pollen abortion in male sterile lines have not been fully elucidated. In addition, there is still no hybrid oat that is commercially planted due to the lack of a suitable system of male sterility for hybrid breeding.ResultsIn this study, we cultivated a male sterile oat line and a near-isogenic line by crossbreeding to elucidate the expression patterns of genes that may be involved in sterility. The first reported CA male sterile (CAMS) oat line was used for cross-testing and hybridization experiments and was confirmed to exhibit a type of nuclear sterility controlled by recessive genes. Oat stamens of two lines were sampled at four different developmental stages separately. Paired-end RNA sequencing was performed for each sample and generated 252.84 Gb sequences. There were 295,462 unigenes annotated in public databases in all samples, and we compared the histological characteristics and transcriptomes of oat stamens from the two oat lines at different developmental stages. Our results demonstrate that the sterility of the male sterile oat line occurs in the early stage of stamen development and is primarily attributable to abnormal meiosis and the excessive accumulation of superoxide.ConclusionsTo the best of our knowledge, this study is the first to decipher the dynamic expression profiles of pollen abortion CAMS and CA male fertile (CAMF) oat lines, which may represent a valuable resource for further studies attempting to understand pollen abortion and anther development in oats.

Modeling emergence of sterile oat (Avena sterilis ssp. ludoviciana) under semiarid conditions

AbstractWinter wild oat [Avena sterilis ssp. ludoviciana (Durieu) Gillet & Magne; referred to as A. sterilis here] is one of the major weed species of the Avena genus, given its high competitive ability to infest cereal crops worldwide, with special concern in Spain. A nine-location field experiment was established across Spain where a total of 400 A. sterilis seeds per location were sowed in four replicates in autumn 2016 to monitor the emergence during two growing seasons in dryland conditions. The data were used to test the prediction ability of previously published thermal (TT) and hydrothermal time (HTT) models and to develop new models, if required. Overall, the average percentage of emergence was 30% during the first season and 21% during the second season. In both seasons, the main emergence flush occurred between November and February. According to the phenological stage, A. sterilis achieved the tillering earlier in southern sites, between November 25 and the end of December, compared with northern sites, where this stage was reached at the end of January. The newly developed model described the emergence with precision, using three cardinal temperatures to estimate the TT. The three cardinal points were established at −1.0, 5.8, and 18.0 C for base (Tb), optimum (To), and ceiling temperature (Tc), while the base water potential (Ψb) was established at −0.2 MPa for the HTT estimation. This study contributes to improving prediction of the emergence of A. sterilis and provides knowledge for decision support systems (DSS) for the control of this weed.

Hidden Diversity of Crown Rust Resistance within Genebank Resources of Avena sterilis L.

The most widespread and damaging fungal disease of the oat plant is crown rust. Resistance to the crown rust pathogen, Puccinia coronata Cda. f. sp. avenae (Pca), at the seedling stage of Avena sterilis accessions from the Polish national genebank was characterised by five North American and Polish pathotypes of Pca of diverse pathogenicity. Pca pathogenicity was determined on a series of 34 differential lines carrying known seedling resistance genes. Seventy-five percent of studied accessions showed a heterogeneous infection pattern, 17% behaved as homogenous susceptibles, and 7% of tested genotypes could be unambiguously described as resistant. This study proved that A. sterilis accessions preserved in a genebank as complex populations could be a very valuable source of resistance to crown rust. The complexity of analysed populations was ascertained by a detailed variance analysis of transformed resistance/susceptibility data. We demonstrate here that hidden sources of resistance may be discovered in accessions with general susceptibility.

Seed longevity and seedling emergence behavior of wild oat (Avena fatua) and sterile oat (Avena sterilis ssp. ludoviciana) in response to burial depth in eastern Australia

AbstractWeed emergence time and the longevity of weed seeds within the soil play an important role in implementing a timely and effective weed control program. In this study, the seed longevity and emergence pattern of wild oat (Avena fatua L.) and sterile oat [Avena sterilis ssp. ludoviciana (Durieu) Gillet & Magne] were monitored in field conditions. Fresh seeds of A. fatua and A. ludoviciana were placed into nylon bags (50 seeds per bag in three replications for three locations in eastern Australia: Gatton, Narrabri, and St George) and buried at depths of 0, 2, and 10 cm in November 2017. Bags were exhumed at 6-mo intervals over 30 mo to evaluate seed germination, viability, and decay components. The seed decay component of A. fatua and A. ludoviciana followed an exponential pattern. On both the surface and at the 10-cm burial depth, 50% of the seeds of A. fatua and A. ludoviciana had decayed by 6 mo. The seeds of A. fatua persisted longer at 2-cm depth than at other depths, particularly at St George, where 90% of the seeds decayed after the 30-mo study. However, at Gatton and Narrabri, 90% of the seeds of A. fatua at this depth had decayed after 18 mo of burial in the soil. In the emergence pattern experiment (2017 to 2019), the emergence of A. fatua and A. ludoviciana from different burial depths was also studied. The emergence of A. fatua and A. ludoviciana was greater from 2-cm (29% to 36%) and 5-cm (18% to 43%) soil depths compared with the surface (5% to 10%) and 10-cm (3-9%) soil depth. Avena ludoviciana emerged earlier (2,253 growing degree days [GDD]; March 14, 2018) than A. fatua (3,364 GDD; May 23, 2018). Both species exhibited high emergence between May to June 2018, and the last cohort of each species was observed in October 2018. The highest seedling emergence occurred at the start of the winter season (May), which emphasizes the need for early PRE weed control such as tillage, herbicide application, and cover crops to ensure crops are planted in a clean seedbed. The continued emergence of these weeds into the spring season (October) emphasizes the need for extended periods of A. fatua and A. ludoviciana management. The results also suggest that management strategies that can control all emerged seedlings over 2 yr and restrict seed rain in the field could lead to complete control of Avena spp. in the field.

Mechanism and pattern of resistance to some ACCase inhibitors in winter wild oat (Avena sterilis subsp. ludoviciana (Durieu) Gillet & Magne) biotypes collected within canola fields

Due to the reports regarding unsuccessful control of Avena sterilis subsp. ludoviciana (Durieu) Gillet & Magne by haloxyfop-R-methyl in canola fields, the following study was conducted to investigate the resistance of this weed to haloxyfop-R-methyl. Five out of 22 accessions were resistant to haloxyfop-R-methyl. These biotypes were then subjected to various rates of clodinafop propargyl, sethoxydim, pinoxaden and mesosulfuron methyl + iodosulfuron-methyl herbicides and their cross-resistance to clodinafop propargyl and sethoxydim was confirmed. However, no resistance was observed to pinoxaden and mesosulfuron-methyl + iodosulfuron-methyl herbicides. Indirect study of metabolism by P450 using 1- aminobenzotriazole and piperonyl butoxide showed that this enzyme had no contribution to occurrence of resistance in the studied biotypes. Allele-specific PCR results indicated that Ile-2041-Asn mutation is responsible for resistance of A. sterilis subsp. ludoviciana biotypes, which was confirmed by sequencing of the samples. Since pinoxaden negatively affects canola, the growers face a serious limitation in their choice for chemical management and thus, implementation of integrated weed management such as introduction of row crops such as faba bean in crop rotation and increasing the diversity of herbicide mode of action by cultivation of crops such as sugar beet in crop rotation may prove helpful. In fields under canola-wheat rotation, it is also possible to use pinoxaden in wheat. Also, trifluralin, cycloxydim and clethodim herbicides may be tested on A. sterilis subsp. ludoviciana. This was the first case of A. sterilis subsp. ludoviciana resistance to ACCase inhibitors in canola fields.

No fitness cost associated with Asn-2041-Ile mutation in winter wild oat (Avena ludoviciana) seed germination under various environmental conditions

Knowledge about the fitness cost imposed by herbicide resistance in weeds is instrumental in devising integrated management methods. The present study investigated the germination response of ACCase-resistant (R) and susceptible (S) winter wild oat under different environmental conditions. The DNA of the plants was sequenced after being extracted and purified. The segregated F2 seeds were subjected to various temperatures, water potentials, NaCl concentrations, different pHs, darkness conditions, and burial depths. The results of the sequencing indicated that Ile-2041-Asn mutation is responsible for the evolution of resistance in the studied winter wild oat plants. The seeds were able to germinate over a wide range of temperatures, osmotic potentials, NaCl concentrations, and pHs. Germination percentage of R and S seeds under dark and light conditions was similar and ranged from 86.3 to 88.3%. The highest emergence percentage for both R and S plants was obtained in 0, 1, and 2 cm depths and ranged from 66.6 to 70.3%. In overall, no differences were observed in the germination response between the R and S winter wild oat plants under all studied conditions. No fitness cost at seed level indicates that control of R winter wild oats is more difficult, and it is essential to adopt crop and herbicide rotation to delay the further evolution of resistance.

Biological traits of six sterile oat biotypes in response to planting time

AbstractSterile oat (Avena ludoviciana Durieu) is a problematic weed in winter crops of Australia. Biotypes of sterile oat adapted to the local environment may vary in environmental plasticity, which could increase their invasiveness. A 2‐yr (winter growing seasons of 2018 and 2019) study in Australia investigated the influence of planting date (early, mid‐, and late season) on the growth and reproduction potential of six biotypes (AL2/17, AL3/17, AL4/17, AL5/17, AL6/17, and AL9/17) of sterile oat. Averaged across biotypes, the seed production of sterile oat was reduced by 21 and 84% for mid‐ and late planting, respectively, compared with early planting (2,660 seeds plant−1). Seed production was highly correlated with the first panicle emergence for early planting. However, for mid‐ and late‐season planting, seed production was correlated with panicle number and plant biomass. For mid‐season planting, taller plants had more seed number plant−1 than shorter plants. The results of this study imply that biotypes of sterile oat differ in environmental plasticity and that they can complete their life cycle under a wide range of temperatures in the winter season and contribute to its reinfestation. Results also suggest that early and mid‐season cohorts are prolific in seed production; however, late cohorts can also produce sufficient seeds for reinfestation. Therefore, integrated weed management strategies must be focussed on those tactics (manipulation of sowing time and row spacing) that could create a competitive crop environment and to suppress the growth of early cohorts of sterile oat to reduce their seed production.

Molecular Characterization of Cereal Yellow Dwarf Virus-RPV in Grasses in European Part of Turkey

Abstract Yellow dwarf viruses (YDVs) are economically destructive viral diseases of cereal crops, which cause the reduction of yield and quality of grains. Cereal yellow dwarf virus-RPV (CYDV-RPV) is one of the most serious virus species of YDVs. These virus diseases cause epidemics in cereal fields in some periods of the year in Turkey depending on potential reservoir natural hosts that play a significant role in epidemiology. This study was conducted to investigate the presence and prevalence of CYDV-RPV in grasses and volunteer cereal host plants including 33 species from Poaceae, Asteraceae, Juncaceae, Geraniaceae, Cyperaceae, and Rubiaceae families in the Trakya region of Turkey. A total of 584 symptomatic grass and volunteer cereal leaf samples exhibiting yellowing, reddening, irregular necrotic patches and dwarfing symptoms were collected from Trakya and tested by ELISA and RT-PCR methods. The screening tests showed that 55 out of 584 grass samples were infected with CYDV-RPV in grasses from the Poaceae family, while none of the other families had no infection. The incidence of CYDV-RPV was detected at a rate of 9.42%. Transmission experiments using the aphid species Rhopalosiphum padi L. showed that CYDV-RPV was transmitted persistently from symptomatic intact grasses such as Avena sterilis, Lolium perenne and Phleum exratum to barley cv. Barbaros seedlings. PCR products of five Turkish RPV grass isolates were sequenced and compared with eleven known CYDV-RPV isolates in the GenBank⁄ EMBL databases. Compared nucleotide and amino acid sequences of CYDV-RPV isolates showed that the identities ranged from 40.38 − 95.86 % to 14.04 − 93.38%, respectively. In this study, 19 grass species from the Poaceae family and two volunteer cereal host plants were determined as natural reservoir hosts of CYDV-RPV in the cereal growing areas of Turkey.

Mechanistic basis for synergism of 2,4-D amine and metribuzin in Avena sterilis.

The combination of herbicides with different modes of action has been adopted not only to improve weed control but also to increase the environmental sustainability of plant-protection products. In this study, we showed a synergistic effect of the auxin herbicide 2,4-D amine with the PSII-inhibiting herbicide metribuzin to control the global grass weed wild oat (Avena sterilis) population and investigated the underlying mechanisms. Pretreatment with 2,4-D amine did not change the foliar absorption of metribuzin but did increase metribuzin translocation to the roots and new leaves, although enhancement of the metribuzin metabolism was also observed. Considering that the expression level of the target site psbA gene is significantly higher in leaves than in roots, increased metribuzin translocation to new leaves is likely the major cause of the observed synergism, even though enhanced metribuzin metabolism may offset the metribuzin efficacy. This is the first report on the synergistic mechanism between 2,4-D amine and metribuzin in weed control.

Weed Flora in Mustard- A review

Among all other oil seed crops mustard is the most important oil seed crop. They play wide role in economy of India. The productivity of mustard is reduced by various biotic and abiotic factors. The weed is one of the major factor to decline the productivity of mustard. The yield losses in mustard upto 45% caused by weeds. Weed flora is the life of plant that is related to weeds and they differ from place to place. The weed flora involve the population of both dicot and monocot weeds. The dicot species are more present than monocot species in mustard. The Chenopodium album, Melilotus indica, Anagallis arvensis, Cirsium arvense, Fumaria parviflora, Cornopus didymus, Phalaris minor, Rumex denticulatus, Vicia sativa, Carthamus oxycantha, Chenopodium murale, Avena ludoviciana, Medicago denticulatus etc. weeds are mostly present in mustard field. Weed flora related with soil characteristics, region, climate, cropping pattern and management factors. The weeds are noxious plant and compete with crop for wetness, light, space, nutrition supplements and reduce the quality and yield attributes of crops.