Autumn Cold Research Articles

Long-term effects of climate change on juvenile bull shark migratory patterns.

Seasonal variability in environmental conditions is a strong determinant of animal migrations, but warming temperatures associated with climate change are anticipated to alter this phenomenon with unknown consequences. We used a 40-year fishery-independent survey to assess how a changing climate has altered the migration timing, duration and first-year survival of juvenile bull sharks (Carcharhinus leucas). From 1982 to 2021, estuaries in the western Gulf of Mexico (Texas) experienced a mean increase of 1.55°C in autumn water temperatures, and delays in autumn cold fronts by ca. 0.5 days per year. Bull shark migrations in more northern estuaries concomitantly changed, with departures 25-36 days later in 2021 than in 1982. Later, migrations resulted in reduced overwintering durations by up to 81 days, and the relative abundance of post-overwintering age 0-1 sharks increased by >50% during the 40-year study period. Yet, reductions in prey availability were the most influential factor delaying migrations. Juvenile sharks remained in natal estuaries longer when prey were less abundant. Long-term declines in prey reportedly occurred due to reduced spawning success associated with climate change based on published reports. Consequently, warming waters likely enabled and indirectly caused the observed changes in shark migratory behaviour. As water temperatures continue to rise, bull sharks in the north-western Gulf of Mexico could forgo their winter migrations in the next 50-100 years based on current trends and physiological limits, thereby altering their ecological roles in estuarine ecosystems and recruitment into the adult population. It is unclear if estuarine food webs will be able to support changing residency patterns as climate change affects the spawning success of forage species. We expect these trends are not unique to the western Gulf of Mexico or bull sharks, and migratory patterns of predators in subtropical latitudes are similarly changing at a global scale.

CONSTRUCTION OF A SCHEME FOR THE USE OF FODDER CROPS FOR THE DRY STEPPE ZONE OF KAZAKHSTAN

Pasture keeping of farm animals in the conditions of the dry steppe zone is a labor-intensive process. To provide stable animals with feed, we have developed the structure of a hay-pasture conveyor. This method is based on the use of forage crops, scientifically based selection of species composition, productivity level, direction of economic purpose. The developed composition uses mixtures of perennial herbs consisting of granary, esparcet and alfalfa, and also uses seeds of Sudanese grass, fodder sorghum. The main attention is drawn to the fact that the crops of Sudanese grass are mown in the "tubing - earing" phase. To obtain a good grass of the Sudanese grass, mowing was carried out at a height of 10-12 cm in the phase of throwing out the panicle. The sowing of fodder sorghum for green fodder was carried out in the second decade of May, since the warm period favorably affects the increase in vegetative mass. In the panicle phase, forage sorghum is recommended to be drained in October-November, and in warm winter conditions it is used as a winter pasture. At the end of the pasture season, the autumn cold comes. During this period, the animals return to the pens again, where feeding is carried out with harvested hay. The developed structure of the hay-pasture conveyor allows for a long time to receive pasture feed and increase the pasture period by 90-95 days due to the presence of annual and perennial grasses in the scheme. The haymaking and pasture conveyor is strongly influenced by weather and climatic conditions, under favorable conditions it is possible to obtain from a unit area from 113.7 to 125 c/ha of pasture grass green mass.

Genetic mapping and identification of a QTL determining tolerance to freezing stress in Fragaria vesca L.

Extreme cold and frost cause significant stress to plants which can potentially be lethal. Low temperature freezing stress can cause significant and irreversible damage to plant cells and can induce physiological and metabolic changes that impact on growth and development. Low temperatures cause physiological responses including winter dormancy and autumn cold hardening in strawberry (Fragaria) species, and some diploid F. vesca accessions have been shown to have adapted to low-temperature stresses. To study the genetics of freezing tolerance, a F. vesca mapping population of 143 seedlings segregating for differential responses to freezing stress was raised. The progeny was mapped using ‘Genotyping-by-Sequencing’ and a linkage map of 2,918 markers at 851 loci was resolved. The mapping population was phenotyped for freezing tolerance response under controlled and replicated laboratory conditions and subsequent quantitative trait loci analysis using interval mapping revealed a single significant quantitative trait locus on Fvb2 in the physical interval 10.6 Mb and 15.73 Mb on the F. vesca v4.0 genome sequence. This physical interval contained 896 predicted genes, several of which had putative roles associated with tolerance to abiotic stresses including freezing. Differential expression analysis of the 896 QTL-associated gene predictions in the leaves and crowns from ‘Alta’ and ‘NCGR1363’ parental genotypes revealed genotype-specific changes in transcript accumulation in response to low temperature treatment as well as expression differences between genotypes prior to treatment for many of the genes. The putative roles, and significant interparental differential expression levels of several of the genes reported here identified them as good candidates for the control of the effects of freezing tolerance at the QTL identified in this investigation and the possible role of these candidate genes in response to freezing stress is discussed.

Adaptive performance of genetically improved and unimproved seedlings of Scots pine

Our main objective was to determine whether various genetically improved reproductive materials of Scots pine ( L.) differ in growth rhythm, autumn cold acclimation and resilience from unimproved materials. The study consisted of two successive indoor experiments with Scots pine seedlings representing four levels of genetic gain (unimproved natural stands, first-generation seed orchards, 1.5-generation seed orchards and seed orchards established with freezing-tested parents) and a wide range of geographical origins within Finland. The seedlings were assessed for terminal shoot elongation, growth cessation, bud set, freezing injuries and bud flushing over the first growth period. All the adaptive traits showed a latitudinal trend regardless of the genetic level. Seed orchard progenies and natural stand progenies did not differ significantly in the timing of growth cessation, bud set, and the flushing rate of the frost-injured seedlings, after the trait variation was adjusted to the latitude of origin. The differences in autumn frost hardiness were insignificant, too, except for the somewhat higher injury rate displayed by the first-generation seed orchard materials. The finding was not conclusive due to ambiguous results from the two experiments. Overall, we did not find evidence of alarming compromises in the adaptive performance of genetically improved materials.Pinus sylvestris

Champions of winter survival: cold acclimation and molecular regulation of cold hardiness in evergreen conifers.

Evergreen conifers are champions of winter survival, based on their remarkable ability to acclimate to cold and develop cold hardiness. Counterintuitively, autumn cold acclimation is triggered not only by exposure to low temperature, but also by a combination of decreasing temperature, decreasing photoperiod and changes in light quality. These environmental cues control a network of signaling pathways that coordinate cold acclimation and cold hardiness in overwintering conifers, leading to cessation of growth, bud dormancy, freezing tolerance and changes in energy metabolism. Advances in genomic, transcriptomic and metabolomic tools for conifers have improved our understanding of how trees sense and respond to changes in temperature and light during cold acclimation and the development of cold hardiness, but there remain considerable gaps deserving further research in conifers. In the first section of this review, we focus on the physiological mechanisms used by evergreen conifers to adjust metabolism seasonally and to protect overwintering tissues against winter stresses. In the second section, we review how perception of low temperature and photoperiod regulate the induction of cold acclimation. Finally, we explore the evolutionary context of cold acclimation in conifers and evaluate challenges imposed on them by changing climate and discuss emerging areas of research in the field.

Diurnal Variations of Low-Level Winds and Precipitation Response to Large-Scale Circulations during a Heavy Rainfall Event

AbstractA succession of MCSs developed during the last week of October 2016 and produced extreme heavy rainfall in central China. The event underwent an evident shift from a mei-yu-like warm scenario to an autumn cold scenario. Diurnal cycles of rainfall and low-level winds may be modulated by the shifting of large-scale atmospheric conditions. We conducted observational analyses and numerical experiments to examine how large-scale circulations influenced rainfall systems through diurnally varying processes. The results show that, in the first half (warm) period of the event, intense rainfall mostly occurred in eastern-central China with an early morning peak. It was closely related to a nocturnal southwesterly low-level jet (NLLJ) on the flank of the western Pacific subtropical high. The NLLJ formed near midnight in southern China where ageostrophic wind rotated clockwise due to Blackadar’s inertial oscillation. The NLLJ extended downstream to central China during the predawn hours due to the horizontal advection of momentum. Both the formation and extension of the NLLJ were supported by an enhanced subtropical high that provided relatively warm conditions with surface heating for boundary layer inertial oscillation and strong background southwesterly winds for momentum transport. The NLLJ induced MCSs at its northern terminus where the low-level ascent, moisture flux convergence, and convective instability were enhanced during the predawn hours. In the second half period with an intrusion of cold air, the diurnal amplitude of low-level winds became small under relatively cold and cloudy conditions. Moderate rainfall tended to occur in western-central China with a peak after midnight, most likely due to frontogenetic processes, upslope lifting, and nighttime cloud-top cooling.

Autumn Cold Surge Paths over North China and the Associated Atmospheric Circulation

Using the Hybrid Single-Particle Lagrangian Integrated Trajectory (HYSPLIT) model, we tracked the paths of 46 autumn cold surges affecting North China from 1961 to 2014, and classified them by clustering analysis, thereby investigating their changes and associated atmospheric circulation evolution. Our results indicate that autumn cold surges affecting North China can be classified into three types according to their paths: the north type, west type, and northwest type, with occurrences of 12, 16, and 18 respectively. Different types of cold surges have different atmospheric circulation characteristics. The north type is associated with a blocking type of atmospheric circulation pattern, with an enhanced stretching northeast ridge over the Ural Mountains and a transverse trough over Lake Baikal. However, the northwest type is characterized by a ridge–trough–ridge wave-train pattern that is located over the Barents Sea, West Siberian Plain, and Sakhalin Island, respectively. The west-type cold surge is related to a conversion type: a blocking system over the Ural Mountains forms four days before the cold surge occurrence, after which it becomes a wave-train type. The atmospheric signals detected prior to the occurrences of the three types of cold surges are also explored. The main signal of the north-path cold surges is that the energy propagates eastward from the Azores Islands to the Ural Mountains, and then forms a blocking high over the Urals. However, for the northwest-path cold surges, there is a weak trough over the Ural Mountains that gradually strengthens because the blocking high collapses over the Norwegian Sea. The key signal of the formation of the west-path cold surges is a blocking high over the Norwegian Sea’s continuous enhancement and extension to Novaya Zemlya, which results in a transmission of energy to the Ural Mountains and leads to the formation of a blocking system over here. When the above-mentioned different types of atmospheric circulation characteristics appear, the type of cold surge path and its impact area can be potentially forecasted in advance, which may reduce the losses that result from cold surges.

Biochemical changes and winter hardiness in pomegranate (Punica granatum L.) trees grown under deficit irrigation

Drought and cold temperature as the most important abiotic factors reduce the agricultural productivity in the world. In this research, the influence of irrigation levels on biochemical changes and cold hardiness of two commercial Iranian pomegranate cultivars (‘Shishecap’ and ‘Malas-Yazdi’) investigated during 2016 and 2017. A factorial experiment based on randomized complete block design was used with three irrigation treatments including full irrigation (control), 75% of crop water requirement (moderate stress) and 50% of crop water requirement (severe stress). At the end of the growing season, some biochemical traits such as proline, soluble carbohydrate, starch, and total phenolic content as well as antioxidant activity and 50% lethal temperature (LT50) were measured in pomegranate stems. Proline, total phenolics, soluble carbohydrate content and antioxidant activity increased and starch content decreased with reducing the irrigation level. The most autumn cold hardiness in November for ‘Shishecap’ (−16.26 °C and −15.56 °C, respectively for the first and second year) and ‘Malas-Yazdi’ (−13.50 °C and −12.33 °C, respectively for the first and second year) was found in control trees, and the lowest cold hardiness for ‘Shishecap’ (−12.63 °C and −11.65 °C, respectively for the first and second year) and ‘Malas-Yazdi’ (−12.85 °C and −11.50 °C, respectively for the first and second year) were recorded in severe stressed plants. Winter cold hardiness in ‘Shishecap’ was affected by irrigation levels during both years; however in ‘Malas-Yazdi’, it was affected by irrigation levels only in the first year of study. Based on the Pearson correlation coefficients, starch content had a positive correlation with cold hardiness at the end of the growing season. Also, correlation coefficients between soluble carbohydrate and LT50 in November (r = 0.82, P ≤ 0.001) were negative. Therefore, it can be concluded that irrigation levels affect the pomegranate trees cold hardiness. Full irrigation resulted in an increase in the cold hardiness in the pomegranate trees, depending on cultivar.

Arctic-North Pacific coupled impacts on the late autumn cold in North America

The Pacific decadal oscillation (PDO) is known to bring an anomalously cold (warm) period to southeastern (northwestern) North America during the cold season of its positive phase through a Rossby wave linkage. This study provides evidence that the remote connection between the North Pacific and the downstream temperature over central North America is strengthened by the warm arctic conditions over the Chukchi and East Siberian Sea, especially in the late autumn season. The modulation effect of the Arctic manifests itself as an altered Rossby wave response to a transient vorticity forcing that results from an equatorward storm track shift, which is induced collaboratively by the PDO and the warm Arctic. This observational finding is supported by two independent modeling experiments: (1) an idealized coupled GCM experiment being nudged toward the warm arctic surface condition and (2) a simple stationary wave model experiment forced by transient eddy forcing.

Association of Taiwan's October rainfall patterns with large-scale oceanic and atmospheric phenomena

The variability of the amount of October rainfall in Taiwan is the highest among all seasons. The October rainfall in Taiwan is attributable to interactions between the northeasterly monsoon and typhoons and their interaction with Taiwan's Central Mountain Range. This study applied long-term gridded rainfall data for defining the major rainfall pattern for October in Taiwan. The empirical orthogonal function Model 1 (80%) of the October rainfall and El Niño Southern Oscillation (ENSO) index exhibited a significant out-of-phase coherence in a 2–4year period band. This is because an easterly flow on the northern edge of an anomalous low-level cyclonic circulation over the South China Sea during a La Niña developing stage increased the occurrence of an autumn cold front and enhanced the northeasterly monsoon toward northern Taiwan. In addition, a southerly flow on the eastern edge of the anomalous cyclone increased the moisture transport from the tropical Pacific toward Taiwan. The warmer sea surface temperature in the South China Sea, Kuroshio, and the subtropical western Pacific, which may have been induced by an ENSO warm phase peak in the preceding winter, promoted the formation of the anomalous low-level cyclonic circulation.

Influence of low- and high-elevation plant genomes on the regulation of autumn cold acclimation in Abies sachalinensis.

Boreal coniferous species with wide geographic distributions show substantial variation in autumn cold acclimation among populations. To determine how this variation is inherited across generations, we conducted a progeny test and examined the development of cold hardening in open-pollinated second-generation (F2) progeny of Abies sachalinensis. The F1 parents had different genetic backgrounds resulting from reciprocal interpopulational crosses between low-elevation (L) and high-elevation (H) populations: L × L, L × H, H × L, and H × H. Paternity analysis of the F2 progeny using molecular genetic markers showed that 91.3% of the fathers were located in surrounding stands of the F1 planting site (i.e., not in the F1 test population). The remaining fathers were assigned to F1 parents of the L × L cross-type. This indicates that the high-elevation genome in the F1 parents was not inherited by the F2 population via pollen flow. The timing of autumn cold acclimation in the F2 progeny depended on the cross-type of the F1 mother. The progeny of H × H mothers showed less damage in freezing tests than the progeny of other cross-types. Statistical modeling supported a linear effect of genome origin. In the best model, variation in freezing damage was explained by the proportion of maternally inherited high-elevation genome. These results suggest that autumn cold acclimation was partly explained by the additive effect of the responsible maternal genome. Thus, the offspring that inherited a greater proportion of the high-elevation genome developed cold hardiness earlier. Genome-based variation in the regulation of autumn cold acclimation matched the local climatic conditions, which may be a key factor in elevation-dependent adaptation.

Foliar Applied Abscisic Acid Increases ‘Chardonnay’ Grapevine Bud Freezing Tolerance during Autumn Cold Acclimation

Economic loss due to cold weather events is a major constraint to winegrape (Vitis vinifera) production and wine-related industries where extreme and/or fluctuating winter temperatures induce injury and require remedial retraining and replanting increases production costs and lowers yield and fruit quality. The purpose of this study was to determine whether a foliar application of abscisic acid (ABA) could increase the freezing tolerance (FT) of field-grown, ‘Chardonnay’ winegrape and whether its effectiveness can be influenced by the phenological timing of the application. Mature ‘Chardonnay’ grapevines were treated with a foliar application of ABA at a concentration of 500 mg·L−1 at vine phenological stages corresponding to 50% véraison, postvéraison, and postharvest. Results from field trial sites located in four distinct winegrape production regions in the United States (Idaho and Ohio) and Canada (British Columbia and Ontario) showed that foliar application of ABA increased bud FT, primarily during autumn cold acclimation. Foliar ABA application had no consistent influence on bud FT in midwinter or during spring deacclimation, or on percent budburst in spring. Vine phenological stage at the time of ABA foliar application influenced ABA effectiveness, although results were inconsistent among locations. At most locations, applications made at véraison or postvéraison were more effective than applications made postharvest. No phytotoxic response or adverse changes in yield or berry composition were detected in response to ABA application. The consistent increase in bud FT during autumn cold acclimation observed at all trial locations in this study indicates that foliar ABA, applied at véraison or postvéraison, can reduce the risk of economic loss due to cold injury in production regions with frequent early autumn cold weather events.

Fruit Ripening Has Little Influence on Grapevine Cold Acclimation

This four-year study tested whether the physiological demand of fruit ripening may interfere with grapevine cold acclimation in autumn or with midwinter hardiness. Three harvest time treatments were established in a mature vineyard of own-rooted Cabernet Sauvignon vines: clusters were removed after fruit set, at veraison, or after the first fall frost. Average yield of the late harvested vines varied from 4.2 to 5.1 kg/vine (7.5 to 9.2 t/ha) among years, and soluble solids varied from 23.4 to 25.6 Brix. The presence of fruit during ripening delayed leaf senescence, measured as chlorophyll decline. The fruit also tended to delay the senescence-associated decrease in photosynthesis. All vines showed typical patterns of autumn cold acclimation, midwinter hardiness, and spring deacclimation. Cold hardiness of buds, cane phloem, and cane xylem varied during winter depending on prevailing temperature, and during the coldest winter reached levels of −27°C for 50% bud damage and −28°C for the onset of xylem injury. Early fruit removal had no effect on cane nonstructural carbohydrates and, with few exceptions, failed to enhance cold hardiness. Depending on the year, early fruit removal improved bud hardiness on 5 to 15% and xylem hardiness on 8 to 38% of all measurement dates. On those dates, the early harvested vines tended to be 1 to 2°C hardier than the late harvested vines, irrespective of the time of crop removal. No trend was found for phloem hardiness. These results indicate that cropping, at least within commercially acceptable limits in regions with sufficiently long or warm growing seasons, rarely impacts cold acclimation and maximum hardiness. Grapevines appear to adjust seasonal leaf physiology to meet their carbon demand for both fruit ripening and cold acclimation.

Genotype and environment: two factors related to autumn cold hardiness on Persian walnut (Juglans regia L.)

J. regia timber is appreciated for high-value wood products. In new plantations, biotic or abiotic events which could affect wood quality should be monitored. Autumn frosts could affect annual shoot development, with consequent loss of timber value or even tree death. In southern Europe, climate change forecasts include erratic and severe autumn frost events. The relationship between genotype and environment regarding susceptibility to autumn frost damage was examined in four provenances of Juglans regia L., planted at two ecologically different sites, one subject to Mediterranean and the other Atlantic weather conditions. Annual budsticks from eight trees per provenance were collected in November 2010 from each site. The samples were then submitted to freeze–thaw cycles down to −8, −13 and −18 °C, plus a control treatment (keeping samples at 5 °C). Damage to the stem was assessed using the index of freezing injury calculated from relative electrolyte leakage at each temperature considered. Frost damage to stem, apical and lateral buds was recorded by visual scoring. Differences in cold acclimation between sites were detected, with provenances exhibiting differences on senescence. A ‘provenance × site’ interaction was found in some of the analyses, but the same ranking of susceptibility was detected for all provenances at both sites and in all the tissues analysed. The differences between provenances could be related to their geographical origins, where an altitude gradient was observed. The genetic component was important in the expression of autumn cold hardiness and, together with productive traits, should be considered in new afforestation projects.

Autumn urea application and cold hardiness of apple trees

Effect of autumn urea application on cold hardiness of apple trees was investigated at Experimental Orchard in Dąbrowice on four apple cultivars ('Szampioii'. ´Jonagold´, ´Elstar' and 'Spartan´) of different sensitivity to low temperature injuries. During three-years experiment (2000-2002) no changes in frost resistance of apple trees after urea treatment in the end of October (during leaves fall) were noted. After the earlier application of urea, at middle of October. significantly more damages of annual shoots of cv. ´Jonagold' occurred only in the season 2001. These damages did not influence later growth of apple trees. Spring observations indicated that for all apple's cultivars setting of buds was better on treated with urea than on untreated ones. Electrolyte leakage determinations confirmed the field results that urea did not cause decrease in low temperature resistance of apple trees.

Genomic and phenotypic architecture of a spruce hybrid zone (Picea sitchensis × P. glauca)

Interspecific hybridization may enhance the capacity of populations to adapt to changing environments, and has practical implications for reforestation. We use genome-wide estimates of admixture and phenotypic traits for trees in a common garden to examine the extent and direction of gene flow across a Picea hybrid zone, testing assumptions of the bounded hybrid superiority and tension zone models of hybrid zone maintenance. Seeds were collected from the ecological transition zone spanning from maritime to continental climates across the Picea sitchensis-P.glauca contact zone, and 721 trees were planted in a common garden experiment within the hybrid zone. Individuals were genotyped using a panel of 384 candidate-gene single nucleotide polymorphisms (SNPs) putatively associated with adaptive traits in Picea, and phenotyped at age ten for height and autumn cold hardiness. Low interspecific heterozygosity in hybrids indicated that intrinsic reproductive barriers were too weak to prevent widespread recombination, although introgression appeared asymmetric with P.sitchensis dominating the zone. Whereas marker-based hybrid index was strongly correlated with climate and geography, phenotypic traits exhibited weak or no significant clines. Our results indicated that exogenous selection appeared to play a strong role in the distribution and structure of this hybrid zone, indicative of an environmentally determined bounded hybrid superiority model of hybrid zone maintenance, although endogenous mechanisms could not be ruled out. This study provides insight into the mechanisms underlying adaptation across ecologically transitional hybrid zones that will ultimately provide an additional tool in managing these economically important tree species.

Inheritance of height growth and autumn cold hardiness based on two generations of full-sib and half-sib families of Pinus sylvestris

The genetic variability in height growth and autumn cold hardiness were evaluated in a full-sib family of Scots pine (Pinus sylvestris (L.)) in a field trial in northern Sweden and 358 open pollinated families generated from the progeny of the full-sib cross. Height and autumn cold hardiness were assessed in the progeny of the full-sib cross at 9–19 years of age and on 3995 open pollinated progenies at three years of age. The autumn cold hardiness [critical temperature (CT)] of trees and progenies was tested by artificial freeze testing of detached needles at various temperatures. Significant genetic variation was found among progenies in both height growth and cold hardiness with a narrow sense heritability of 0.16 and 0.37, respectively. There was also a significant positive genetic correlation (r a =0.39) between height growth and CT with taller trees showing later autumn cold acclimation. It was concluded that there is significant additive genetic variance for height and CT which warrants a potential for mapping quantitative trait loci. Furthermore, the low heritability for height growth shows the importance of removing the environmental influence by using additive genetic predictions instead of phenotypic measurements thereby improving the accuracy of genetic associations.

Metabolic dynamics during autumn cold acclimation within and among populations of Sitka spruce (Picea sitchensis)

• Autumnal cold acclimation in conifers is a complex process, the timing and extent of which vary widely along latitudinal gradients for many tree species and reflect local adaptation to climate. Although previous studies have detailed some aspects of the metabolic remodelling that accompanies cold acclimation in conifers, little is known about global metabolic dynamics, or how these changes vary among phenotypically divergent populations. • Using untargeted GC-MS metabolite profiling, we monitored metabolic dynamics during autumnal cold acclimation in three populations of Sitka spruce from the southern, central, and northern portions of the species range, which differ in both the timing and extent of cold acclimation. • Latitudinal variation was evident in the nature, intensity, and timing of metabolic events. Early development of strong freezing tolerance in the northern population was associated with a transient accumulation of amino acids. By late autumn, metabolic profiles were highly similar between the northern and central populations, whereas profiles for the southern population were relatively distinct. • Our results provide insight into the metabolic architecture of latitudinal adaptive variation in autumn acclimation and show that different mechanisms are the basis of early October cold hardiness and autumn-acclimated cold hardiness.

Widespread, ecologically relevant genetic markers developed from association mapping of climate‐related traits in Sitka spruce (Picea sitchensis)

• Genecological studies in widespread tree species have revealed steep genetic clines along environmental gradients for climate-related traits. In a changing climate, the ecological and economic importance of conifers necessitates an appraisal of how molecular genetic variation shapes quantitative trait variation, and one of the most promising approaches to answer this question is association mapping. • We phenotyped a wide collection of 410 individuals of the widely distributed conifer Sitka spruce rangewide (Picea sitchensis) for budset timing and autumn cold hardiness, and genotyped these individuals for a panel of 768 single nucleotide polymorphisms (SNPs) representing > 200 expressed nuclear genes. • After correcting for population structure, associations were detected in 28 of the candidate genes, which cumulatively explained 28 and 34% of the phenotypic variance in cold hardiness and budset, respectively. Most notable among the associations were five genes putatively involved in light signal transduction, the key pathway regulating autumn growth cessation in perennials. Many SNPs with phenotypic associations were also correlated with at least one climate variable. • This study represents a significant step toward the goal of characterizing the genomic basis of adaptation to local climate in conifers, and provides an important resource for breeding and conservation genetics in a changing climate.

Norway Spruce (Picea abies [L.] Karst.) Provenance Variation in Autumn Cold Hardiness: Adaptation or Acclimation?

Norway Spruce (Picea abies [L.] Karst.) Provenance Variation in Autumn Cold Hardiness: Adaptation or Acclimation?