This paper presents detailed comparative studies to control the separately excited DC motor speed. The configurations of proposed methods are fuzzy auto-tuning PID and Adaptive Neuro-fuzzy inference system. The study intends to optimize the parameters of the transient speed response, for instance, the overshoot, settling time and rise time. Also, a comparison has been made between the two suggested controllers. PID tuned by Ziegler Nicholas’s method utilized as a basis for the proposed controllers. Furthermore, PID model is used to extract the data set for ANFIS configuration, which has been manipulated by genetic algorithm and particle swarm optimization to ensure that it will be able to cover all operation condition possibilities. In order to investigate the eligibility of the suggested controllers, the model of the DC Motor is tested under several conditions. Finally, the results which implemented using Matlab-Simulink toolbox showed that fuzzy auto-tuning PID controller is too complicated, and has slow dynamic response comparing with ANFIS controller. Moreover, ANFIS has rapid robustness efficiency, also in the domain of the motor response characteristics. ANFIS expressed superior proficiency and better Keywords: SEDC motor, fuzzy auto-tuning PID, Adaptive Neuro-fuzzy inference system (ANFIS), genetic algorithm, particle swarm optimization, data set, and FIS. Performance under various experimental conditions.