According to recent statistics, the number of service workers in the industry exceeded 11,714,000 in 2017 to 13,825,000 in 2021, with about 4,217,000 in manufacturing and 1,651,000 in construction, the largest number of service workers in the industry. In addition, it is confirmed that the incidence of industrial accidents by size is the highest among those with less than 50 employees. The roadmap for reducing serious accidents recently announced by the Ministry of Employment and Labor aims to reduce industrial accidents through autonomous safety and health management of companies, such as the spread of safety awareness and safety culture within the organization. Based on previous studies that the role of workers in the organization is important for the organization's safety culture, and safety leadership affects organizational safety culture and safety behavior, this study attempted to confirm how safety leadership in the service sector affects safety culture and safety behavior. Safety leadership is an independent variable, and 206 workers in the service sector at workplaces with more than 50 employees and workplaces with less than 50 employees were examined to determine how it affects safety behavior, such as well as procedures, consciousness, and attitudes. A confirmatory factor analysis was conducted to secure compatibility and reliability of the tool, and the Cronbach's alpha value was derived. In addition, frequency analysis and descriptive statistics analysis were performed to understand the characteristics of the study subject and major variables, and Pearson correlation analysis was used to determine the correlation between major variables. The mediating effect was analyzed using the Process macro, and the significance of the mediating effect was confirmed through the Bootstrapping-test. The significance of all statistical results was based on 0.05. As a result of the study, safety leadership was found to have a forward effect on safety culture and safety behavior, but more than 50 people and less than 50 people showed different patterns. In other words, safety leadership directly affected safety culture and safety behavior in workplaces with more than 50 employees, but safety leadership directly affected safety culture in workplaces with less than 50 employees.Through the results of this study, the research and policy suggestions necessary in the future are as follows. First, a detailed study on the sub-factors of each variable is needed. Second, it is necessary to identify additional variables that affect the safety culture of the service industry by size and prepare measures to spread and improve the safety culture in institutional and step-by-step ways.