With the automotive industry rising rapidly, the world confronts many significant environmental and fossil fuel depletion challenges at a higher rate. This resulted in the creation for many researchers of a reprocessing and ecological replacing petrol. Biodiesel is one alternative of this kind. Biodegradability, low volatility, high levels of cetane, and no sulphur emissions are strengthening the case. While many experiments with a broad variety of non-eatable oils were done in many gasoline mixtures in this area, all of those tests are carried out primarily in farm compression engines. That was our report on Gossypium arboreum oil (GAO) development, through a transesterification of Gossypium arboreum seed oil and through a thorough test and comparative review of the performance, combustion and emissions of automotive engines with different biodiesel combinations produced to serve as a fuel.