Autologous Platelet-rich Plasma Injection Research Articles

Subtenon Injection of Autologous Platelet-Rich Plasma in Retinitis Pigmentosa: Is It a New Therapeutic Option?

Purpose: Growth factors (GFs) and neurotrophins can decelerate retinal degeneration. This study aimed to investigate the safety, efficacy and durability of subtenon injections of autologous platelet-rich plasma (aPRP) which is a rich source of GFs in retinitis pigmentosa (RP) patients. Methods: 154 eyes of 77 RP patients with various degrees of narrowed visual field were investigated in this study. Each patient received three injections with 4-week intervals and followed for at least 12 months. The examinations and the tests were obtained before the injection, 1 month after the third injection and every 3 months during the study. The primary aim was to evaluate the effects of aPRP on visual acuity (VA) and visual functions, the second aim was to estimate the duration of the therapy effect and the need for additional aPRP injection. Results: Median age of the 77 RP patients was 35.2 ± 13.9 years. All of the eyes received 3 monthly bilateral subtenon aPRP injections. Of these patients 26 received an additional one, 12 received additional two and 1 patient received additional 3 injections with 3-month interval. There were no significant ocular or systemic side effects. The mean baseline VA was 0.22 ± 0.18 Snellen lines. It improved to 0.31 ± 0.19 following three aPRP injections, which was statistically significant. At the end of the study period, the mean VA was 0.27 ± 0.22 Snellen lines. Conclusion: The subtenon injection of aPRP might be an effective therapy and might have a positive influence in the preservation of visual functions and visual acuity of RP patients.

Clinical study of local injection of autologous platelet-rich plasma in treatment of diabetic foot ulcer

To investigate the effectiveness of local injection of autologous platelet-rich plasma (PRP) in treatment of diabetic foot ulcer. Between October 2017 and October 2018, 90 diabetic foot ulcer patients who met the selection criteria were randomly divided into 3 groups: PRP injection group (group A, PRP was injected and hydrogel dressing covered the wounds), PRP covered group (group B, PRP gel and hydrogel dressing covered the wounds), and the control group (group C, hydrogel dressing covered the wounds), 30 cases in each group. There was no significant difference in gender, age, injured side, disease duration, preoperative glycosylated hemoglobin, wound size, and Wagner grading between groups (P>0.05). The frequency of treatments and hospitalization day in all groups and the total amount of PRP application in groups A and B were recorded. The wound healing condition was recorded during the treatment, and the wound healing rate was calculated at 3 months after the first debridement. The frequency of treatments in groups A, B, and C were (10.2±0.8), (11.4±0.6), (12.5±0.5) times, respectively. The total amount of PRP application of groups A and B were (306±24) and (342±18) mL, respectively. There was no significant difference in the frequency of treatments and the total amount of PRP application between groups (P>0.05). The hospitalization days of groups A, B, and C were (40.5±1.8), (62.1±2.3), and (88.6±1.4) days, respectively, showing significant differences between groups (P<0.05). In the course of treatment, the necrosis and exudation of the wounds gradually reduced, the areas of wounds gradually reduced; and the above conditions of group A were significantly better than groups B and C, and group B was better than group C. At 3 months after the first debridement, the wound healing rates of groups A, B, and C were 93.2%±0.8%, 52.1%±1.1%, and 21.3%±1.3%, respectively, with significant differences between groups (P<0.05). PRP can effectively promote the repair of diabetic foot ulcer. The effectiveness of local injection of PRP is superior to the local coverage.

Autologous Platelet-Rich Plasma in the Treatment of Perianal Fistula in Crohn’s Disease

AimTo assess clinical healing in patients with perianal Crohn’s disease with local intrafistular injection of autologous platelet-rich plasma. MethodThe pilot study was conducted at a single centre between January 2013 and December 2015. Autologous platelet-rich plasma was prepared in platelet-rich and platelet-poor fractions for local intrafistular injection in patients with proven, established perianal Crohn’s disease. Patients were permitted biological therapies, and the Perianal Crohn’s Disease Activity Index was recorded. Patients were followed for 48 weeks for clinical signs of healing (complete, partial or non-healing), monitoring fistula drainage, closure and epithelialization. ResultsThe study included 29 patients (19 males; mean age 38 ± 12.8 years) with four exclusions in the operating room because surgery was not indicated and four lost to follow-up. Five adverse events were recorded, with two requiring the drainage of abscess collections. Of the 21 patients assessable at 24 weeks, there was complete healing, partial healing and non-healing in 7 (33.3%), 8 (38.1%) and 6 (28.6%) patients, respectively. By 48 weeks, there was complete healing, partial healing and non-healing in 6 (40%), 6 (40%) and 3 (20%) patients, respectively, with a reduction in the number of visible external fistula openings at both time points (P = 0.021). By the end of the study, there was a higher trend of healing if biological therapies were continued (85.7% with biologics vs. 75% without, P = 0.527), but there were no statistically significant differences and no differences in the Perianal Crohn’s Disease Activity Index. ConclusionAutologous platelet-rich plasma is safe in patients with perianal Crohn’s disease, with an acceptable healing rate over a medium-term follow-up, particularly if biological therapies are used concomitantly.

Effect of Autologous Platelet-rich Plasma on Appearance of Tenocytes at Injured Achilles Tendon Entheses in Rabbits.

To determine the effects of autologous platelet-rich plasma on appearance of tenocytes at injured Achilles tendon entheses in rabbits. Laboratory-based experimental study. Department of Anatomy and Pathology, Army Medical College, Rawalpindi, in collaboration with National Institute of Health, Islamabad, from March to May, 2018. Forty healthy male, 4-6 months old New Zealand white rabbits, weighing 2000-2500 gm, were randomly divided into four equal groups A, B, C and D. Group A served as control, while B, C and D were experimental groups. Injury was surgically induced at left Achilles tendon entheses of experimental groups, which were treated with injections of autologous platelet-rich plasma, at the time of induction of injury in group C and two weeks after injury in group D, while no treatment was given in group B. Animals were euthanised at the end of 12 weeks. Specimens were taken and processed for histological observations. Bonar's modified score was used to assess the appearance of tenocytes. SPSS version 21 was used for statistical analysis. Chi-square test was applied and p-value of <0.05 was taken as significant. Experimental group B showed marked deterioration in Bonar's score as compared to the control group A. The scores were significantly improved in treatment groups C and D as compared to group B. Injection of autologous platelet-rich plasma effectively improved the Bonar's modified score for appearance of tenocytes at injured entheses, 12 weeks after injury.

Is Platelet-rich Plasma Injection more Effective than Steroid Injection in the Treatment of Chronic Plantar Fasciitis in Achieving Long-term Relief?

Introduction: Plantar fasciitis is characterised by pain in the heel, which is aggravated on weight bearing after prolonged rest. Many modalities of treatment are commonly used in the management of plantar fasciitis including steroid injection. Many studies show that steroid injection provides pain relief in the short term but not long lasting. Recent reports show autologous platelet-rich plasma (PRP) injection promotes healing, resulting in better pain relief in the short as well as long term. The present study was undertaken to compare the effects of local injection of platelet-rich plasma and Corticosteroid in the treatment of chronic plantar fasciitis.Materials and methods: Patients with the clinical diagnosis of chronic plantar fasciitis (heel pain of more than six weeks) after failed conservative treatment and plantar fascia thickness more than 4mm were included in the study. Patients with previous surgery for plantar fasciitis, active bilateral plantar fasciitis, vascular insufficiency or neuropathy related to heel pain, hypothyroidism and diabetes mellitus were excluded from the study. In this prospective double-blind study, 60 patients who fulfilled the criteria were divided randomly into two groups. Patients in Group A received PRP injection and those in Group B received steroid injection. Patients were assessed with visual analog scale (VAS) and American Orthopedic Foot and Ankle Society (AOFAS) score. Assessment was done before injection, at six weeks, three months and six months follow-up after injection. Plantar fascia thickness was assessed before the intervention and six months after treatment using sonography.Results: Mean VAS in Group A decreased from 7.14 before injection to 1.41 after injection and in Group B decreased from 7.21 before injection to 1.93 after injection, at final follow-up. Mean AOFAS score in Group A improved from 54 to 90.03 and in Group B from 55.63 to 74.67 at six months’ follow-up. The improvements observed in VAS and AOFAS were statistically significant. At the end of six months’ follow-up, plantar fascia thickness had reduced in both groups (5.78mm to 3.35mm in Group A and 5.6 to 3.75 in Group B) and the difference was statistically significant.Conclusion: Local injection of platelet-rich plasma is an effective treatment option for chronic plantar fasciitis when compared with steroid injection with long lasting beneficial effect.

Is PRP Effective in Reducing the Scar Width of Primary Cleft Lip Repair? A Randomized Controlled Clinical Study.

To evaluate and analyze the effect of platelet-rich plasma (PRP) injection on the scar formed after unilateral complete cleft lip scar repair using a modified Millard technique. An unavoidable cheiloplasty scar is a result of the wound healing process that not only influences patient self-esteem for life but also affects muscle function. Blind, randomized, controlled clinical trial. From December 2016 to February 2018, 24 patients with unilateral complete cleft lip undergoing primary cheiloplasties were equally assigned to study and control groups. All patients were treated by modified Millard cheiloplasty. In the study group, PRP was injected into the muscle and skin layers immediately after wound closure, while the control group patients were treated with no PRP injection. Scar width was assessed after 6 months through the muscle using ultrasonography and at the skin surface via photographs. Scar width showed a significant improvement in the study group. Injection of autologous PRP provides effective improvement of cutaneous and muscular wound healing and decreases scar tissue formation.

A comparative study of combining intra-articular injection of platelet-rich plasma or sodium hyaluronate with isotonic muscle strength training in treating knee osteoarthritis

Objective To compare the efficacy of intra-articular injection of autologous platelet-rich plasma (PRP) with that of sodium hyaluronate for patients with knee osteoarthritis (KOA). Methods Fifty KOA patients were randomly divided into a PRP group (n=25) and a sodium hyaluronate group (n=25). Those in the PRP group were injected intra-articularly with 5 ml of autologous PRP every 2 weeks for 6 consecutive weeks, while the sodium hyaluronate group was given intra-articular injections of 2 ml of sodium hyaluronate once a week for 6 weeks. Both groups performed isokinetic strength training three times a week for 6 weeks. Before as well as 6 weeks, 3 months, 6 months and 12 months after the treatment, both groups were followed and evaluated using the Western Ontario and McMaster University osteoarthritis index (WOMAC). A Biodex System 4 isometric muscle strength testing system was used to evaluate the knees′ peak torque (PT) in flexion and extension, total power (TW), and average power (AP). Results Before the treatment there were no significant differences in pain, stiffness or average WOMAC score between the two groups. Afterward significant improvement was observed in the pain, stiffness and WOMAC scores of both groups. Compared with the sodium hyaluronate group, significantly greater decreases in the average WOMAC pain score were observed in the PRP group after the different intervals. That was also true of the average stiffness scores and the overall average WOMAC scores after 6 and 12 months. The flexor and extensor PT, TW and AP at the angular velocities of 60°/s and 90°/s also improved significantly in both groups after 6 weeks and 12 months, with the improvement of the PRP group significantly greater than that in the control group at the same time points. Conclusion Intra-articular injection of either autologous PRP or sodium hyaluronate can effectively alleviate the clinical symptoms of KOA when combined with isokinetic strength training. However, PRP is the more effective. Its use is worthy of clinical application and promotion. Key words: Knees; Osteoarthritis; Plasma; sodium hyaluronate; Isokinetic strength

Efficacy, safety and tolerability of autologous platelet rich plasma injection in the treatment of lateral epicondylitis

&lt;p class="abstract"&gt;&lt;strong&gt;Background:&lt;/strong&gt; To evaluate the effects of platelet-rich plasma (PRP) infiltration in patients with lateral epicondylitis of the elbow.&lt;/p&gt;&lt;p class="abstract"&gt;&lt;strong&gt;Methods:&lt;/strong&gt; A randomized, prospective study on 60 patients with lateral epicondylitis of the elbow was conducted at Ruby Hall Clinic, Pune. The patients were randomized and evaluated after receiving infiltration of three milliliters of PRP, or methyl prednisolone. The base-line evaluation was done using visual analog score (VAS) and modified Mayo performance index for elbow (MAYO). Re-evaluation was after 1 and 6 months of the procedure. Statistical analysis was done using independent t-test.&lt;strong&gt;&lt;/strong&gt;&lt;/p&gt;&lt;p class="abstract"&gt;&lt;strong&gt;Results:&lt;/strong&gt; After 6 months of treatment with PRP, patients with lateral epicondylitis had a statistically significant improvement in their VAS (p&amp;lt;0.05) in contrast to steroid. However, no statistical difference in modified Mayo performance index was found between the two groups at 1 and 6 months after intervention.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusions:&lt;/strong&gt; Treatment of patients with lateral epicondylitis with PRP reduces pain and is better tolerated than steroid therapy. Thus, the novel PRP therapy can be considered as a primary approach to treat patients of lateral epicondylitis conservatively. &lt;/p&gt;

Assessment of effectiveness of intra articular autologous platelet rich plasma injection in the management of adhesive capsulitis of shoulder joint

Background: It was reported that platelet rich plasma injection is effective in the management of pain in adhesive capsulitis. Platelet rich plasma injection has multiple uses in the field of medical sciences. Objectives: The present study was undertaken to observe the effectiveness of intra articular autologous platelet rich plasma injection in the management of adhesive capsulitis of shoulder joint. Methods: The present study involved 30 patients within the age group of 30 to 60 years of age were included. Both males (n=12) and females (n=18) were present in the study. DASH score and visual analogue scores were assessed in the patients with 8 weeks follow up. Data was collected pre injection, post injection after four weeks. Results: There was a significant decrease in the scores of DASH and Visual analogue scale scores followed by the intervention.

Clinical study of ultrasound-guided intra-articular injection of autologous platelet rich plasma for the treatment of lumbar facet joint syndrome

Objective To investigate the clinical efficacy of ultrasound-guided intra-articular injection of autologous platelet rich plasma (PRP) for lumbar facet joint. Methods A total of 40 patients with back pain caused by lumbar facet joint syndrome were enrolled in this study according to the inclusion criteria. They were assigned into two groups (n=20) using the method of random number table to receive the treatment of intra-articular injection of autologous PRP (P group) and glucocorticoid combined with local anesthetic treatment (S group). The therapeutic effects at postoperative (immediately, 1 week, 1 month, 3 months, 6 months) were evaluated by using the Visual Analogue Scale (VAS) score, remission rate of pain [(VAS score before operation-VAS score after operation)/VAS score before operation] and the number of patients with pain relief rate (≥50%). In addition, the adverse reactions, such as aggravation of pain, symptoms of local or systemic infection, symptoms related to nerve injury, hemorrhage and fever from the immediately end after injection to one week after treatment of the two groups were also compared. Results The S group has lower VAS score, higher pain relief rate and more numbers of patients with pain relief rate≥50% than that of P group at postoperative (immediately, 1 week, 1 month)(P<0.05). However, the P group has lower VAS score, higher pain relief rate and more numbers of patients with pain relief rate≥50% than that of S group at 3 months and 6 months after operation (P<0.05). Both groups didn′t show the adverse reactions immediately and 1 week after operation. Conclusions The clinical efficacy of ultrasound-guided intra-articular injection of autologous PRP for lumbar facet joint is not better than glucocorticoid combined with local anesthetic injection at the time of end of operation, 1 week and 1 month after the treatment, but is better than the effect at the time points of 3 months and 6 months after the treatment. Key words: Lumbar facet joint syndrome; Platelet rich plasma; Glucocorticoid

Live Birth Rates in Poor Responders' Group after Previous Treatment with Autologous Platelet-Rich Plasma and Low Dose Ovarian Stimulation Compared with Poor Responders Used Only Low Dose Ovarian Stimulation Before in Vitro Fertilization.

BACKGROUND:This prospective pilot study determined the efficacy of previous transvaginal intraovarian injection with autologous platelet-rich plasma (PRP) in poor ovarian responders (PORs) fulfilling the Bologna criteria before in vitro fertilisation (IVF)/intracytoplasmic sperm injection (ICSI) with low dose ovarian stimulation. Current knowledge of efficient treatment for PORs is limited and often contradictory; also, LBRs of IVF remains disappointingly low.AIM:We assessed the live birth rates (LBRs) in PORs after previous ovarian treatment with PRP.METHODS:Overall, 40 patients undergoing IVF/ICSI between June 2017 ending December 2018 were included. A transvaginal intraovarian injection of PRP was performed on 20 patients. Both compered groups were balanced for all basic characteristics, and multivariate analysis was performed to adjust for all known confounders.RESULTS:Between the groups, a statistical significance in clinical pregnancies and LBR was not found. Clinical pregnancy and live birth rates were 33.33 ± 44.99 and 40.00 ± 50.71 in the PRP group and 10.71 ± 28.95 and 14.29 ± 36.31 in control group retrospectively. However, there is a trend towards higher implantation rates and LBRs in patients with previous treatment with PRP. Anyhow, the number of patients used in the research is insufficient to make a concrete conclusion, and more studies are needed in the future to confirm these results entirely.CONCLUSION:Even though the treatment of POR responders remains as a therapeutical challenge, the usage of intraovarian injection of autologous PRP in PORs before the IVF performance brings a glimpse of new hope in increasing the success of IVF defined by clinical pregnancy and LBRs.

Treatment of complex recurrent fistula-in-ano by surgery combined to autologous bone marrow–derived mesenchymal stroma cells and platelet-rich plasma injection

We report a case of successful management of complex recurrent cryptoglandular fistula-in-ano by surgery combined with autologous bone marrow-derived mesenchymal stroma cells (MSCs) and platelet-rich plasma (PRP) injection. Clinical, radiological, and surgical data of the patient were reviewed, as well as the current literature on complex fistula-in-ano. A 37-year-old man with a recurrent cryptoglandular perianal fistula was addressed to our department. Inflammatory bowel disease was excluded by clinical history, endoscopy, and blood tests. Physical examination and MRI showed an anterior external orifice on the midline, 5cm from the anal verge, with an internal orifice on the same line. Surgery combined to injection of MSC-PRP solution was successfully performed. MSC-PRP solution was prepared while the patient was under general anesthesia: bone marrow MSCs were obtained by centrifugation of a tibial puncture specimen and PRP from a peripheral whole blood sample of the patient. There were no adverse events post-operatively. Clinical and MRI examination 4months after treatment confirmed the absence of perianal fistula. More than 2years after surgery, there has been no recurrence. Treatment of complex recurrent cryptoglandular fistula-in-ano by surgery combined to autologous bone marrow-derived MSCs and PRP injection seems safe in selected patients, allowing long-term healing. This procedure seems promising but further evaluation by clinical trials is warranted.

Autologous platelet-rich plasma therapy for pattern hair loss: A systematic review.

Androgenetic alopecia (male pattern and female pattern hair loss) is characterized by thinning of the scalp hair. Intradermal injection of autologous platelet-rich plasma (PRP) might have an effect on hair regrowth. The aim was to evaluate efficacy and safety of platelet-rich plasma compared to placebo or no treatment in people with pattern hair loss. We searched the databases CENTRAL and MEDLINE on December 24, 2018 and included randomized controlled trials (RCTs). Primary outcomes were mean change of hair density from baseline and serious treatment-related adverse events. Secondary outcome was mean change of hair thickness from baseline. Time point of outcome assessment was 6months after start of treatment. We identified 13 relevant randomized controlled trials with 356 randomized (343 analyzed) people or half-head areas who received PRP in a simple parallel or half-head design. The pooled data of seven studies (171 analyzed people or half-head areas) were favorable in the PRP group on hair density. We estimated a mean difference from baseline of 30.35 associated with a wide 95% confidence interval (1.77-58.93), a considerable heterogeneity (I2 =100%), and unclear risk of bias in most of the studies. Regarding hair thickness, data were also favorable in the PRP group, but these data were limited to a single study. We did not identify serious treatment-related adverse events. The results of seven RCTs indicated that autologous platelet-rich plasma was associated with an increase of hair density when compared to placebo.

Efficacy of platelet-rich plasma plus fractional carbon dioxide laser in treating posttraumatic scars.

The management of posttraumatic scars is a great challenge. Autologous platelet-rich plasma (APRP) and fractional carbon dioxide (CO2) laser can be applied as innovative treatments for posttraumatic scars. The aim of this study was to evaluate the efficacy, safety and complications of fractional CO2 laser exposure plus APRP injection in treating traumatic scars versus CO2 laser applied as a monotherapy. Twenty participants were treated 3 times at a 4-week interval with a follow-up visit. The scars were divided into an upper and lower half-treated with fractional CO2 laser exposure plus APRP injection and CO2 laser alone, respectively. The scar was evaluated using the Vancouver scar scale (VSS), along with patient satisfaction. Twenty patients completed the 3 month treatment course. The mean VSS score improved significantly from 7.0 ± 3.97 to 3.25 ± 1.55 and 6.75 ± 1.77 to 3.85 ± 1.59 with fractional CO2 laser exposure plus APRP injection and CO2 laser exposure, respectively (p < .001). However, CO2 laser plus APRP was significantly superior to CO2 laser alone (p = .001), with fewer adverse effects. Our study shows that combined treatment with CO2 laser exposure plus APRP injection is safer and more effective for posttraumatic scar management than CO2 laser exposure alone.

Management of Deep Retinal Capillary Ischemia by Electromagnetic Stimulation and Platelet-Rich Plasma: Preliminary Clinical Results.

To investigate the efficacy of retinal electromagnetic stimulation and sub-tenon autologous platelet-rich plasma in the treatment of deep retinal capillary ischemia. The study included 28 eyes of 17 patients aged 15-76years (mean 37.9years) who had deep retinal capillary ischemia. Patients who had acute-onset paracentral scotoma in the last 1month were included in the study between January 2018 and January 2019. The diagnosis of deep retinal capillary ischemia was based on clinical history and typical findings of optical coherence tomography angiography. The eyes were divided into three groups: group 1 (n = 7 eyes) received electromagnetic stimulation alone; group 2 (n = 7 eyes) received electromagnetic stimulation and sub-tenon autologous platelet-rich plasma injection; group 3 had no intervention and served as a control group (n = 14 eyes). The patients underwent ten sessions of electromagnetic stimulation in groups 1 and 2. Sub-tenon autologous platelet-rich plasma injection was performed immediately after the first, fifth, and tenth sessions of electromagnetic stimulation in group 2. The deep retinal capillary density and best corrected visual acuity changes were investigated before and after treatment at the first month. The mean deep retinal capillary density was 52.0% before electromagnetic stimulation and 56.1% after ten sessions of application in group 1; this improvement was statistically significant (p = 0.01). In the combined treatment group (group 2), the mean deep retinal capillary density was 46.9% before the treatment and 56.5% after the treatment; this increase was also statistically significant (p = 0.01). Statistically significant best corrected visual acuity improvement (p = 0.01) could be achieved only in group 2. The combined treatment was significantly superior (p < 0.01) to treatment with only electromagnetic stimulation regarding best corrected visual acuity and deep retinal capillary density. In the control group (group 3), there was no statistically significant change (p = 0.09) in the mean deep retinal capillary density and best corrected visual acuity. Treatment of the underlying cause is a priority in the treatment of deep retinal capillary ischemia. However, in the acute period, local ischemia treatment is necessary to prevent permanent retinal damage and scotomas. In mild cases, only electromagnetic stimulation, which is non-invasive and easy to use, might have a beneficial effect on deep retinal capillary density. In more severe cases, sub-tenon fresh autologous platelet-rich plasma injection together with electromagnetic stimulation may be more effective in the treatment of local ischemia of the retina in order to augment the response. The Rapid Service Fees were funded by the Ankara University Tecnopolis Institute. titck.gov.tr identifier, 2018-136.

A study on management of tennis elbow by local platelet rich plasma injection

&lt;p class="abstract"&gt;&lt;strong&gt;Background:&lt;/strong&gt; Lateral epicondylitis (tennis elbow), a familiar term used to describe myriad symptoms around the lateral aspect of the elbow can occur during activities that require repetitive supination and pronation of the forearm with the elbow in near full extension. This condition can cause severe discomfort to the patient resulting in debilitation and impairment of routine activities. The purpose of this study was to evaluate the effectiveness of local autologous platelet rich plasma injection in the treatment of tennis elbow.&lt;/p&gt;&lt;p class="abstract"&gt;&lt;strong&gt;Methods:&lt;/strong&gt; This was a prospective observational study conducted on 50 patients of either sex with an average age of 45.92 years, presenting to the Orthopaedic OPD of SHKM Government Medical College Hospital, Nalhar, NUH, Haryana between November 2016 and February 2018, with a diagnosis of lateral epicondylitis. All the patients were treated with local platelet rich plasma injection and the results were analysed through the assessment of visual analog score (VAS) and disability of arm shoulder and hand (DASH) score. The patients were followed up for a period of 6 months after the local injection of platelet rich plasma.&lt;strong&gt;&lt;/strong&gt;&lt;/p&gt;&lt;p class="abstract"&gt;&lt;strong&gt;Results:&lt;/strong&gt; Majority of the patients had significant relief with this method. The VAS and DASH score improved from the pre-treatment values of 8.7 and 74.6 to 2.6 and 29.8 respectively, which was found to be statistically significant (p&amp;lt;0.001).&lt;/p&gt;&lt;p class="abstract"&gt;&lt;strong&gt;Conclusions:&lt;/strong&gt; Thus results of our study demonstrate that the local injection of platelet rich plasma is a safe and effective method of treatment of lateral epicondylitis.&lt;/p&gt;

Treatment of atrophic acne scars using autologous platelet‐rich plasma vs combined subcision and autologous platelet‐rich plasma: A split‐face comparative study

Multiple therapeutic approaches are usually required when treating atrophic acne scars. Subcision was reported to be of value in improving rolling scars. Autologous platelet-rich plasma (PRP) has recently been proposed as an adjuvant treatment option for atrophic acne scars with few reports evaluating its efficacy. Our objective was to compare the effect of intradermal injection of PRP vs combined PRP and subcision in the treatment of atrophic acne scars. Thirty patients with bilateral atrophic acne scars were enrolled. Each patient received three monthly sessions. Each side of the face was randomly treated either with intradermal PRP alone or with combined treatment with subcision followed by PRP injection. Patients were assessed at 3 and 6months following the last treatment session. Evaluation of serial photographs was performed by two blinded investigators. Platelet-rich plasma alone showed a better response, fewer side effects, and shorter downtime compared to combined subcision and PRP. Autologous PRP injection can be a therapeutic option in the treatment of atrophic acne scars, with fewer complications and better tolerability than combined subcision and autologous PRP.

Assessment of Efficacy of Platelet-Rich Plasma Injection for Facial Rejuvenation

AbstractBackground: Facial rejuvenation includes different ther-apeutic modalities of returning facial youthfulness. PRP injection delivers very high concentrations of growth factors that lead to accelerated tissue regeneration and remodeling.Aim of Study: The aim of this work was to evaluate the efficacy of single session of intradermal injection of autologous Platelet Rich Plasma (PRP) in facial rejuvenation.Patients and Methods: 20 subjects with different types of facial wrinkles were included in this study. They were selected from Dermatology and Venereology Department, Tanta University Hospitals. All of them received single session of intradermal injection of autologous PRP and were clinically assessed before and 2 months after PRP injection using Wrinkle Severity Rating Scale (WSRS), Skin Texture Scale, and Subject Satisfaction Scale.Results: All subjects showed significant clinical improve-ment of all types of facial wrinkles after PRP injection. At the end of follow-up period, there was statistically significant decrease in Wrinkle Severity Rating Scale (WSRS) comparable to that before PRP injection. Additionally, there was a statis-tically significant improvement of skin texture and subject satisfaction after PRP injection.Conclusion: Intradermal injection of autologous PRP is well tolerated and capable of rejuvenating the face and pro-ducing a significant correction of different types of facial wrinkles.

EFFECT OF AUTOLOGOUS PLATELET-RICH PLASMA ON ARRANGEMENT OF COLLAGEN FIBERS AT INJURED ACHILLES TENDON ENTHESES IN RABBITS

Objective: To evaluate the effects of autologous platelet-rich plasma on arrangement of collagen fibers at injured Achilles tendon entheses in rabbits.&#x0D; Study Design: Laboratory-based experimental study.&#x0D; Place and Duration of Study: Department of Anatomy, Army Medical College, Rawalpindi, in collaboration with National Institute of Health, Islamabad, from March to May, 2018.&#x0D; Material and Methods: Forty, healthy, male, “New Zealand white rabbits”, 4-6 months of age, weighing 2000- 2500g, were randomly divided into 4 equal groups (A, B, C and D). Group A served as control, while B, C and D were experimental groups. Injuries were surgically induced at Achilles tendon entheses of rabbits of experimental groups, which were treated with injections of autologous platelet-rich plasma, either at the time of inducing injury or two weeks after injury in groups C and D respectively while group B was left untreated. Animals were sacrificed after 12 weeks. Tissues were processed and stained for histological evaluation. Statistical analysis was done using SPSS version 22. A p-value of ≤0.05 was taken as significant.&#x0D; Results: Experimental group B showed marked deterioration in Bonar’s modified score for arrangement of collagen fibers as compared to the control group A. The scores were significantly improved in treatment groups C and D as compared to group B.&#x0D; Conclusions: Injection of autologous platelet-rich plasma effectively improved the Bonar modified score for arrangement of collagen fibers at injured entheses, 12 weeks after injury as compared to non-treatment group.

Functional outcome of ultrasound guided platelet rich plasma injection and corticosteroid injection for lateral epicondylitis

Platelet Rich Plasma (PRP) has created a huge significance in several medical grounds, including orthopaedics. Numerous studies have shown that Platelet Rich Plasma can be used in the management of bony as well as soft tissue injuries. Lately, Platelet Rich Plasma has been used for chronic enthesopathies like tennis elbow, plantar fasciitis, in sports medicine and also in cartilage regeneration. In this study we assessed and reported the efficacy of ultrasound guided autologous Platelet Rich Plasma and Corticosteroid injection