Introduction: Bicruciate knee ligament injury is a lesion affecting both the posterior cruciate ligament (PCL) and the anterior cruciate ligament (ACL) simultaneously, which is one of the most severe knee joint injuries. Operative simultaneous reconstruction is the standard of treatment. Objectives: To evaluate clinical results of one-stage ACL and PCL reconstruction using autogenous tendon grafts with an all-inside technique. Material and methods: The medical records of patients operated on by a group of surgeons at the Orthopedic Center, Military Hospital 103 from March 2015 to March 2020 were retrospectively analyzed. Thirty-four patients (24 males, 10 females) with combined rupture of both ACL and PCL underwent one-stage ACL and PCL reconstruction using autogenous hamstring and peroneus longus tendon grafts with all-inside technique. Results: The mean time of follow-up was 32.09 ± 25.15 months (9 - 68 months). The mean age was 37.7 ± 9.0 years (range 23 - 56 years). At the final follow-ups, all patients were without limit extension, 5/34 patients (14.71%) with limit flexion. The Lachman test was negative in 32 patients (94.12%), the pivot shift testing in 32 patients (94.12%) was negative, 26 patients (76.47%) had a positive posterior drawer test. The Lysholm knee score preoperative and final follow-up were 46.76 ± 17.08 (range 12 - 82) and 89.29 ± 8.57 (range 50 - 99) (p < 0.01), respectively. Conclusion: This study demonstrates the effectiveness and safety of simultaneous arthroscopically assisted reconstruction of both ACL and PCL using autogenous hamstring and peroneus longus tendon for restoring knee stability with an all-inside technique.