As a result of the work done, the biotechnology of growing Australian red claw crayfish (Cherax quadricarinatus) juvenile in recirculating aquaculture system facilities was developed. The research work was carried out in the laboratory of advanced technologies in aquaculture on the basis of the business incubator the Federal State-Funded Educational Institution of Higher Education “Kuban State University”. The composition of the material base necessary for the implementation of biotechnology is determined, growth rates are determined, an optimal diet is formed and optimal planting densities are determined. For the working process two recirculating aquaculture system units were used. As food we used: minced fish, sturgeon food, artemia nauplii, oatmeal, frozen bloodworm, dried gammarus. During 80 days, decades-long survival varied from 83.6 to 91.8%. The average weight of individuals had changed from 0.03 to 5.10 g. The coefficient of variation of this feature was in the permissible range and increased from 9.1 to 22.6%. The total feed coefficient at a cost of 3387.3 g of feed per increase of 1347.7 g of biomass composed 2.5 units. Using the data obtained, it is possible to make primary calculations when organizing the cultivation of ARCC juveniles.