Auditory Stream Research Articles

The influence of binaural cues on the promotion or inhibition of auditory stream segregation

Though much is known about detection thresholds for binaural cues (interaural time [ITD] and level differences [ILD], and interaural correlation [IAC]), less is known about how these cues influence the separation of auditory sources. The ABA auditory stream segregation paradigm (Bregman, 1990) in a bistable configuration, elicits roughly equivalent proportions of integrated and segregated percepts, where listeners spontaneously switch back and forth between the two. To determine the influence of binaural cues we periodically (25 s) manipulated the binaural cue carried by the A components of the ABA triplet (made of narrowband noise, 6-semitone separation) while maintaining the B component at a stable binaural cue. Participants continuously indicated their perception via button box. Analyzed as a function of binaural cue, lateral cues were more likely to be reported as segregated than midline cues. Binaural cue boundaries (perceptual transition point) were defined using logarithmic regression and were significantly correlated for ILD and ITD (r2 = 0.65, p < 0.05). IAC modulation resulted in significantly more switches when both the A and B components were centered (IAC = 1) than when the IAC of the A component was more diffuse (IAC < 0.9). These results provide evidence of how listeners functionally use binaural cues to segregate auditory streams.

The effects of aging and musicianship on the use of auditory streaming cues.

Auditory stream segregation, or separating sounds into their respective sources and tracking them over time, is a fundamental auditory ability. Previous research has separately explored the impacts of aging and musicianship on the ability to separate and follow auditory streams. The current study evaluated the simultaneous effects of age and musicianship on auditory streaming induced by three physical features: intensity, spectral envelope and temporal envelope. In the first study, older and younger musicians and non-musicians with normal hearing identified deviants in a four-note melody interleaved with distractors that were more or less similar to the melody in terms of intensity, spectral envelope and temporal envelope. In the second study, older and younger musicians and non-musicians participated in a dissimilarity rating paradigm with pairs of melodies that differed along the same three features. Results suggested that auditory streaming skills are maintained in older adults but that older adults rely on intensity more than younger adults while musicianship is associated with increased sensitivity to spectral and temporal envelope, acoustic features that are typically less effective for stream segregation, particularly in older adults.

Auditory stream segregation of amplitude-modulated narrowband noise in cochlear implant users and individuals with normal hearing.

Voluntary stream segregation was investigated in cochlear implant (CI) users and normal-hearing (NH) listeners using a segregation-promoting objective approach which evaluated the role of spectral and amplitude-modulation (AM) rate separations on stream segregation and its build-up. Sequences of 9 or 3 pairs of A and B narrowband noise (NBN) bursts were presented which differed in either center frequency of the noise band, the AM-rate, or both. In some sequences (delayed sequences), the last B burst was delayed by 35 ms from their otherwise-steady temporal position. In the other sequences (no-delay sequences), the last B bursts were temporally advanced from 0 to 10 ms. A single interval yes/no procedure was utilized to measure participants’ sensitivity () in identifying delayed vs. no-delay sequences. A higher value showed the higher ability to segregate the A and B subsequences. For NH listeners, performance improved with each spectral separation. However, for CI users, performance was only significantly better for the condition with the largest spectral separation. Additionally, performance was significantly poorer for the largest AM-rate separation than for the condition with no AM-rate separation for both groups. The significant effect of sequence duration in both groups indicated that listeners made more improvement with lengthening the duration of stimulus sequences, supporting the build-up effect. The results of this study suggest that CI users are less able than NH listeners to segregate NBN bursts into different auditory streams when they are moderately separated in the spectral domain. Contrary to our hypothesis, our results indicate that AM-rate separation may interfere with the segregation of streams of NBN. Additionally, our results add evidence to the literature that CI users build up stream segregation at a rate comparable to NH listeners, when the inter-stream spectral separations are adequately large.

Cortical tracking of voice pitch in the presence of multiple speakers depends on selective attention.

Voice pitch carries linguistic and non-linguistic information. Previous studies have described cortical tracking of voice pitch in clean speech, with responses reflecting both pitch strength and pitch value. However, pitch is also a powerful cue for auditory stream segregation, especially when competing streams have pitch differing in fundamental frequency, as is the case when multiple speakers talk simultaneously. We therefore investigated how cortical speech pitch tracking is affected in the presence of a second, task-irrelevant speaker. We analyzed human magnetoencephalography (MEG) responses to continuous narrative speech, presented either as a single talker in a quiet background or as a two-talker mixture of a male and a female speaker. In clean speech, voice pitch was associated with a right-dominant response, peaking at a latency of around 100 ms, consistent with previous electroencephalography and electrocorticography results. The response tracked both the presence of pitch and the relative value of the speaker’s fundamental frequency. In the two-talker mixture, the pitch of the attended speaker was tracked bilaterally, regardless of whether or not there was simultaneously present pitch in the speech of the irrelevant speaker. Pitch tracking for the irrelevant speaker was reduced: only the right hemisphere still significantly tracked pitch of the unattended speaker, and only during intervals in which no pitch was present in the attended talker’s speech. Taken together, these results suggest that pitch-based segregation of multiple speakers, at least as measured by macroscopic cortical tracking, is not entirely automatic but strongly dependent on selective attention.

Tracking transitional probabilities and segmenting auditory sequences are dissociable processes in adults and neonates.

Since speech is a continuous stream with no systematic boundaries between words, how do pre-verbal infants manage to discover words? A proposed solution is that they might use the transitional probability between adjacent syllables, which drops at word boundaries. Here, we tested the limits of this mechanism by increasing the size of the word-unit to four syllables, and its automaticity by testing asleep neonates. Using markers of statistical learning in neonates' EEG, compared to adult behavioral performances in the same task, we confirmed that statistical learning is automatic enough to be efficient even in sleeping neonates. We also revealed that: (1) Successfully tracking transition probabilities (TP) in a sequence is not sufficient to segment it. (2) Prosodic cues, as subtle as subliminal pauses, enable to recover words segmenting capacities. (3) Adults' and neonates' capacities to segment streams seem remarkably similar despite the difference of maturation and expertise. Finally, we observed that learning increased the overall similarity of neural responses across infants during exposure to the stream, providing a novel neural marker to monitor learning. Thus, from birth, infants are equipped with adult-like tools, allowing them to extract small coherent word-like units from auditory streams, based on the combination of statistical analyses and auditory parsing cues. RESEARCH HIGHLIGHTS: Successfully tracking transitional probabilities in a sequence is not always sufficient to segment it. Word segmentation solely based on transitional probability is limited to bi- or tri-syllabic elements. Prosodic cues, as subtle as subliminal pauses, enable to recover chunking capacities in sleeping neonates and awake adults for quadriplets.

Speech Tracking in Complex Auditory Scenes with Differentiated In- and Out-Field-Of-View Processing in Hearing Aids.

In naturalistic auditory scenes, relevant information is rarely concentrated at a single location, but rather unpredictably scattered in- and out-field-of-view (in-/out-FOV). Although the parsing of a complex auditory scene is a fairly simple job for a healthy human auditory system, the uncertainty represents a major issue in the development of effective hearing aid (HA) processing strategies. Whereas traditional omnidirectional microphones (OM) amplify the complete auditory scene without enhancing signal-to-noise-ratio (SNR) between in- and out-FOV streams, directional microphones (DM) may greatly increase SNR at the cost of preventing HA users to perceive out-FOV information. The present study compares the conventional OM and DM HA settings to a split processing (SP) scheme differentiating between in- and out-FOV processing. We recorded electroencephalographic data of ten young, normal-hearing listeners who solved a cocktail-party-scenario-paradigm with continuous auditory streams and analyzed neural tracking of speech with a stimulus reconstruction (SR) approach. While results for all settings exhibited significantly higher SR accuracies for attended in-FOV than unattended out-FOV streams, there were distinct differences between settings. In-FOV SR performance was dominated by DM and SP and out-FOV SR accuracies were significantly higher for SP compared to OM and DM. Our results demonstrate the potential of a SP approach to combine the advantages of traditional OM and DM settings without introduction of significant compromises.

The effect of age and hearing sensitivity at frequencies above 8 kHz on auditory stream segregation and speech perception.

The effects of age and mild hearing loss over the extended high-frequency (EHF) range from 9000 to 16 000 Hz on speech perception and auditory stream segregation were assessed using four groups: (1) young with normal hearing threshold levels (HTLs) over both the conventional and EHF range; (2) older with audiograms matched to those for group 1; (3) young with normal HTLs over the conventional frequency range and elevated HTLs over the EHF range; (4) older with audiograms matched to those for group 3. For speech in quiet, speech recognition thresholds and speech identification scores did not differ significantly across groups. For monosyllables in noise, both greater age and hearing loss over the EHF range adversely affected performance, but the effect of age was much larger than the effect of hearing status. Stream segregation was assessed using a rapid sequence of vowel stimuli differing in fundamental frequency (F0). Larger differences in F0 were required for stream segregation for the two groups with impaired hearing in the EHF range, but there was no significant effect of age. It is argued that impaired hearing in the EHF range is associated with impaired auditory function at lower frequencies, despite normal audiometric thresholds at those frequencies.

Explicit Instructions Do Not Enhance Auditory Statistical Learning in Children With Developmental Language Disorder: Evidence From Event-Related Potentials.

A current issue in psycholinguistic research is whether the language difficulties exhibited by children with developmental language disorder [DLD, previously labeled specific language impairment (SLI)] are due to deficits in their abilities to pick up patterns in the sensory environment, an ability known as statistical learning (SL), and the extent to which explicit learning mechanisms can be used to compensate for those deficits. Studies designed to test the compensatory role of explicit learning mechanisms in children with DLD are, however, scarce, and the few conducted so far have led to inconsistent results. This work aimed to provide new insights into the role that explicit learning mechanisms might play on implicit learning deficits in children with DLD by resorting to a new approach. This approach involved not only the collection of event-related potentials (ERPs), while preschool children with DLD [relative to typical language developmental (TLD) controls] were exposed to a continuous auditory stream made of the repetition of three-syllable nonsense words but, importantly, the collection of ERPs when the same children performed analogous versions of the same auditory SL task first under incidental (implicit) and afterward under intentional (explicit) conditions. In each of these tasks, the level of predictability of the three-syllable nonsense words embedded in the speech streams was also manipulated (high vs. low) to mimic natural languages closely. At the end of both tasks’ exposure phase, children performed a two-alternative forced-choice (2-AFC) task from which behavioral evidence of SL was obtained. Results from the 2-AFC tasks failed to show reliable signs of SL in both groups of children. The ERPs data showed, however, significant modulations in the N100 and N400 components, taken as neural signatures of word segmentation in the brain, even though a detailed analysis of the neural responses revealed that only children from the TLD group seem to have taken advantage of the previous knowledge to enhance SL functioning. These results suggest that children with DLD showed deficits both in implicit and explicit learning mechanisms, casting doubts on the efficiency of the interventions relying on explicit instructions to help children with DLD to overcome their language difficulties.

Event-Related Potential Evidence for Involuntary Consciousness During Implicit Memory Retrieval.

Classical notion claims that a memory is implicit if has nothing to do with consciousness during the information retrieval from storage, or is otherwise explicit. Here, we demonstrate event-related potential evidence for involuntary consciousness during implicit memory retrieval. We designed a passive oddball paradigm for retrieval of implicit memory in which an auditory stream of Shepard tones with musical pitch interval contrasts were delivered to the subjects. These contrasts evoked a mismatch negativity response, which is an event-related potential and a neural marker of implicit memory, in the subjects with long-term musical training, but not in the subjects without. Notably, this response was followed by a salient P3 component which implies involvement of involuntary consciousness in the implicit memory retrieval. Finally, source analysis of the P3 revealed moving dipoles from the frontal lobe to the insula, a brain region closely related to conscious attention. Our study presents a case of involvement of involuntary consciousness in the implicit memory retrieval and suggests a potential challenge to the classical definition of implicit memory.

Audition controls the flow of visual time during multisensory perception

SummaryPrevious work addressing the influence of audition on visual perception has mainly been assessed using non-speech stimuli. Herein, we introduce the Audiovisual Time-Flow Illusion in spoken language, underscoring the role of audition in multisensory processing. When brief pauses were inserted into or brief portions were removed from an acoustic speech stream, individuals perceived the corresponding visual speech as “pausing” or “skipping”, respectively—even though the visual stimulus was intact. When the stimulus manipulation was reversed—brief pauses were inserted into, or brief portions were removed from the visual speech stream—individuals failed to perceive the illusion in the corresponding intact auditory stream. Our findings demonstrate that in the context of spoken language, people continually realign the pace of their visual perception based on that of the auditory input. In short, the auditory modality sets the pace of the visual modality during audiovisual speech processing.

Oscillatory correlates of linguistic prediction and modality effects during listening to auditory-only and audiovisual sentences

In natural listening situations, understanding spoken sentences requires interactions between several multisensory to linguistic levels of information. In two electroencephalographical studies, we examined the neuronal oscillations of linguistic prediction produced by unimodal and bimodal sentence listening to observe how these brain correlates were affected by the sensory streams delivering linguistic information. Sentence contexts which were strongly predictive of a particular word were ended by a possessive adjective matching or not the gender of the predicted word. Alpha, beta and gamma oscillations were investigated as they were considered to play a crucial role in the predictive process. During the audiovisual or auditory-only listening to sentences, no evidence of word prediction was observed. In contrast, in a more challenging listening situation during which bimodal audiovisual streams switched to unimodal auditory stream, gamma power was sensitive to word prediction based on prior sentence context. Results suggest that prediction spreading from higher sentence levels to lower word levels is optional during unimodal and bimodal sentence listening and is observed when the listening situation is more challenging. Alpha and beta oscillations were found to decrease when semantically constraining sentences were delivered in the audiovisual modality in comparison with the auditory-only modality. Altogether, our findings bear major implications for our understanding of the neural mechanisms that support predictive processing in multimodal language comprehension.

Pupil-Linked Arousal Response Reveals Aberrant Attention Regulation among Children with Autism Spectrum Disorder.

Autism spectrum disorder (ASD) is a developmental disorder that is characterized by difficulties with social interaction and interpersonal communication. It has been argued that abnormal attentional function to exogenous stimuli precedes and contributes to the core ASD symptoms. Notably, the locus ceruleus (LC) and its noradrenergic projections throughout the brain modulate attentional function, but the extent to which this locus ceruleus-norepinephrine (LC-NE) system influences attention in individuals with ASD, who frequently exhibit dysregulated alerting and attention orienting, is unknown. We examined dynamic attention control in girls and boys with ASD at rest using the pupil dilation response (PDR) as a noninvasive measure of LC-NE activity. When gender- and age-matched neurotypical participants were passively exposed to an auditory stream, their PDR decreased for recurrent stimuli but remained sensitive to surprising deviant stimuli. In contrast, children with ASD showed less habituation to recurrent stimuli as well as a diminished phasic response to deviants, particularly those containing social information. Their tonic habituation impairment predicts their phasic orienting impairment, and both impairments correlated with the severity of ASD symptom. Because of the fact that these pupil-linked responses are observed when individuals passively listen without any task engagement, our findings imply that the intricate and dynamic attention allocation mechanism, mediated by the subcortical LC-NE system, is impaired in ASD.SIGNIFICANCE STATEMENT Autistic individuals show attentional abnormalities to even simple sensory inputs, which emerge even before formal diagnosis. One possible mechanism behind these abnormalities is a malfunctioning pacemaker of their attention system, the locus ceruleus-norepinephrine pathway. Here we found, according to the pupillary response (a noradrenergic activity proxy), autistic children are hypersensitive to repeated sounds but hyposensitive to surprising deviant sounds when compared with age-matched controls. Importantly, hypersensitivity to repetitions predicts hyposensitivity to deviant sounds, and both abnormalities positively correlate to the severity of autistic symptoms. This provides strong evidence that autistic children have faulty noradrenergic regulation, which might underly the attentional atypicalities previously evidenced in various cortical responses in autistic individuals.

Lip movements enhance speech representations and effective connectivity in auditory dorsal stream

Viewing speaker's lip movements facilitates speech perception, especially under adverse listening conditions, but the neural mechanisms of this perceptual benefit at the phonemic and feature levels remain unclear. This fMRI study addressed this question by quantifying regional multivariate representation and network organization underlying audiovisual speech-in-noise perception. Behaviorally, valid lip movements improved recognition of place of articulation to aid phoneme identification. Meanwhile, lip movements enhanced neural representations of phonemes in left auditory dorsal stream regions, including frontal speech motor areas and supramarginal gyrus (SMG). Moreover, neural representations of place of articulation and voicing features were promoted differentially by lip movements in these regions, with voicing enhanced in Broca's area while place of articulation better encoded in left ventral premotor cortex and SMG. Next, dynamic causal modeling (DCM) analysis showed that such local changes were accompanied by strengthened effective connectivity along the dorsal stream. Moreover, the neurite orientation dispersion of the left arcuate fasciculus, the bearing skeleton of auditory dorsal stream, predicted the visual enhancements of neural representations and effective connectivity. Our findings provide novel insight to speech science that lip movements promote both local phonemic and feature encoding and network connectivity in the dorsal pathway and the functional enhancement is mediated by the microstructural architecture of the circuit.

Towards auditory stream segregation in Tursiops truncatus

In humans, understanding a voice amidst competing sounds depends on parsing the sound mixture into “streams” representing each source's content. Streaming can be influenced by top-down attentional focus, while acoustic features can affect streaming percepts through bottom-up, automatic processing of pitch, timbre, and location. Dolphins regularly navigate a cacophony of echoes generated during echolocation, conspecific echolocation clicks, and other, passively received sounds (eg. communication signals, environmental noise), and thus, may rely on streaming. Using auditory evoked potentials (AEPs), we asked whether dolphins exhibit evidence of bottom-up, frequency-based stream segregation. Initial results using a classic A-B-A sequence of repeated tone triplets (either low-high-low or high-low-high tones) suggest that the triplets break apart perceptually into low and high streams; specifically, the AEP magnitude evoked by the middle tone increases with increasing frequency separation. Differences in dolphin hearing sensitivity across the frequencies tested seem to account for some of the frequency manipulation effect. Additionally, earlier studies suggest that the dolphin auditory temporal integration window decreases as frequency increases, which may interact with streaming processes. This lays a foundation for future tests of top-down effects on dolphin auditory stream processing such as attention and expectation.

Dynamics of alpha suppression and enhancement may be related to resource competition in cross-modal cortical regions

In the face of multiple sensory streams, there may be competition for processing resources in multimodal cortical areas devoted to establishing representations. In such cases, alpha oscillations may serve to maintain the relevant representations and protect them from interference, whereas theta band activity may facilitate their updating when needed. It can be hypothesized that these oscillations would differ in response to an auditory stimulus when the eyes are open or closed, as intermodal resource competition may be more prominent in the former than in the latter case. Across two studies we investigated the role of alpha and theta power in multimodal competition using an auditory task with the eyes open and closed, respectively enabling and disabling visual processing in parallel with the incoming auditory stream. In a passive listening task (Study 1a), we found alpha suppression following a pip tone with both eyes open and closed, but subsequent alpha enhancement only with closed eyes. We replicated this eyes-closed alpha enhancement in an independent sample (Study 1b). In an active auditory oddball task (Study 2), we again observed the eyes open/eyes closed alpha pattern found in Study 1 and also demonstrated that the more attentionally demanding oddball trials elicited the largest oscillatory effects. Theta power did not interact with eye status in either study. We propose a hypothesis to account for the findings in which alpha may be endemic to multimodal cortical areas in addition to visual ones.

The Effect of Subjective Fatigue on Auditory Processing in Musicians and Nonmusicians

We assessed fatigue's effect on temporal resolution and speech perception in noise abilities in trained instrumental musicians. In a pretest-posttest quasiexperimental research design, trained instrumental musicians (n = 39) and theater artists as nonmusicians (n = 37) participated. Fatigue was measured using a visual analog scale (VAS) under eight fatigue categories. The temporal release of masking measured the temporal resolution, and auditory stream segregation assessed speech perception in noise. Entire testing was carried out at two time-points: before and after rehearsal. Each participant rehearsed for five to six hours: musicians playing musical instruments and theater artists conducted stage practice. The results revealed significantly lower VAS scores for both musicians and nonmusicians after rehearsal, indicating that both musicians and nonmusicians were fatigued after rehearsal. The musicians had higher scores for temporal release of masking and lower scores for auditory stream segregation abilities than nonmusicians in the pre-fatigue condition, indicating musicians’ edge in auditory processing abilities. However, no such differences in the scores of musicians and nonmusicians were observed in the post-fatigue testing. The results were inferred as the music training related advantage in temporal resolution, and speech perception in noise might have been reduced due to fatigue. In the end, we recommend that musicians consider fatigue a significant factor, as it might affect their performance in auditory processing tasks. Future researchers must also consider fatigue as a variable while measuring auditory processing in musicians. However, we restricted the auditory processing to temporal resolution and speech perception in noise only. Generalizing these results to other auditory processes requires further investigation.

Can visual capture of sound separate auditory streams?

In noisy contexts, sound discrimination improves when the auditory sources are separated in space. This phenomenon, named Spatial Release from Masking (SRM), arises from the interaction between the auditory information reaching the ear and spatial attention resources. To examine the relative contribution of these two factors, we exploited an audio-visual illusion in a hearing-in-noise task to create conditions in which the initial stimulation to the ears is held constant, while the perceived separation between speech and masker is changed illusorily (visual capture of sound). In two experiments, we asked participants to identify a string of five digits pronounced by a female voice, embedded in either energetic (Experiment 1) or informational (Experiment 2) noise, before reporting the perceived location of the heard digits. Critically, the distance between target digits and masking noise was manipulated both physically (from 22.5 to 75.0 degrees) and illusorily, by pairing target sounds with visual stimuli either at same (audio-visual congruent) or different positions (15 degrees offset, leftward or rightward: audio-visual incongruent). The proportion of correctly reported digits increased with the physical separation between the target and masker, as expected from SRM. However, despite effective visual capture of sounds, performance was not modulated by illusory changes of target sound position. Our results are compatible with a limited role of central factors in the SRM phenomenon, at least in our experimental setting. Moreover, they add to the controversial literature on the limited effects of audio-visual capture in auditory stream separation.

Regularity of background auditory scene and selective attention: A brain oscillatory study

There are scarce data related to the facilitative effects of regularity encoding on auditory selective attention during every day listening conditions. Therefore, present study aimed to investigate how temporal and spectral regularities of background auditory stream affect auditory selective attention at both levels of brain neural oscillatory activities and involved cortical locations. EEG was recorded in healthy young adults listening to two concurrent auditory streams including background and foreground ones in three conditions differing for background auditory stream which was characterized by having spectral or temporal sound regularities and random structure. The neural sources of EEG bands during recognizing target sounds in the foreground stream were determined via standardized Low-Resolution brain Electromagnetic Tomography (sLORETA). Sound regularities of the background auditory stream had no significant effect on the EEG relative power during the task of selective attention. In all conditions, there was a significant increase in the relative power of EEG alpha and beta frequency bands. sLORETA localized significant increase of mentioned bands in the precuneus of parietal lobe; medial frontal gyrus of frontal lobe and insula of sub-lobar in temporal regularity, spectral regularity and random conditions respectively. These results revealed that although temporal and spectral acoustic regularities of competitor auditory stream had no facilitative effect on alpha-related brain processing during selective attention, different brain cortical locations were activated with the introduction of these regularities. This result might provide preliminary evidence for some degree of brain neural specialization in the processing of temporal and spectral regularities during auditory selective attention tasks.

The Impact of Pitch and Timbre Cues on Auditory Grouping and Stream Segregation.

In multi-talker listening environments, the culmination of different voice streams may lead to the distortion of each source’s individual message, causing deficits in comprehension. Voice characteristics, such as pitch and timbre, are major dimensions of auditory perception and play a vital role in grouping and segregating incoming sounds based on their acoustic properties. The current study investigated how pitch and timbre cues (determined by fundamental frequency, notated as F0, and spectral slope, respectively) can affect perceptual integration and segregation of complex-tone sequences within an auditory streaming paradigm. Twenty normal-hearing listeners participated in a traditional auditory streaming experiment using two alternating sequences of harmonic tone complexes A and B with manipulating F0 and spectral slope. Grouping ranges, the F0/spectral slope ranges over which auditory grouping occurs, were measured with various F0/spectral slope differences between tones A and B. Results demonstrated that the grouping ranges were maximized in the absence of the F0/spectral slope differences between tones A and B and decreased by 2 times as their differences increased to ±1-semitone F0 and ±1-dB/octave spectral slope. In other words, increased differences in either F0 or spectral slope allowed listeners to more easily distinguish between harmonic stimuli, and thus group them together less. These findings suggest that pitch/timbre difference cues play an important role in how we perceive harmonic sounds in an auditory stream, representing our ability to group or segregate human voices in a multi-talker listening environment.

Amateur singing benefits speech perception in aging under certain conditions of practice: behavioural and neurobiological mechanisms.

Limited evidence has shown that practising musical activities in aging, such as choral singing, could lessen age-related speech perception in noise (SPiN) difficulties. However, the robustness and underlying mechanism of action of this phenomenon remain unclear. In this study, we used surface-based morphometry combined with a moderated mediation analytic approach to examine whether singing-related plasticity in auditory and dorsal speech stream regions is associated with better SPiN capabilities. 36 choral singers and 36 non-singers aged 20-87years underwent cognitive, auditory, and SPiN assessments. Our results provide important new insights into experience-dependent plasticity by revealing that, under certain conditions of practice, amateur choral singing is associated with age-dependent structural plasticity within auditory and dorsal speech regions, which is associated with better SPiN performance in aging. Specifically, the conditions of practice that were associated with benefits on SPiN included frequent weekly practice at home, several hours of weekly group singing practice, singing in multiple languages, and having received formal singing training. These results suggest that amateur choral singing is associated with improved SPiN through a dual mechanism involving auditory processing and auditory-motor integration and may be dose dependent, with more intense singing associated with greater benefit. Our results, thus, reveal that the relationship between singing practice and SPiN is complex, and underscore the importance of considering singing practice behaviours in understanding the effects of musical activities on the brain-behaviour relationship.