Attractive Traits Research Articles

Respiratory reoxidation of NADH is a key contributor to high oxygen requirements of oxygen-limited cultures of Ogataea parapolymorpha.

While thermotolerance is an attractive trait for yeasts used in industrial ethanol production, oxygen requirements of known thermotolerant species are incompatible with process requirements. Analysis of oxygen-sufficient and oxygen-limited chemostat cultures of the facultatively fermentative, thermotolerant species Ogataea parapolymorpha showed its minimum oxygen requirements to be an order of magnitude larger than those reported for the thermotolerant yeast Kluyveromyces marxianus. High oxygen requirements of O. parapolymorpha coincided with a near absence of glycerol, a key NADH/NAD+ redox-cofactor-balancing product in many other yeasts, in oxygen-limited cultures. Genome analysis indicated absence of orthologs of the Saccharomyces cerevisiae glycerol-3-phosphate-phosphatase genes GPP1 and GPP2. Co-feeding of acetoin, whose conversion to 2,3-butanediol enables reoxidation of cytosolic NADH, supported a 2.5-fold increase of the biomass concentration in oxygen-limited cultures. An O. parapolymorpha strain in which key genes involved in mitochondrial reoxidation of NADH were inactivated did produce glycerol, but transcriptome analysis did not reveal a clear candidate for a responsible phosphatase. Expression of S. cerevisiae GPD2, which encodes NAD+-dependent glycerol-3-phosphate dehydrogenase, and GPP1 supported increased glycerol production by oxygen-limited chemostat cultures of O. parapolymorpha. These results identify dependence on respiration for NADH reoxidation as a key contributor to unexpectedly high oxygen requirements of O. parapolymorpha.

Similarity in Indicators of Attractiveness in Heterosexual Couples, and their Relationship with Satisfaction and Trust

In our species, the formation and maintenance of romantic partners is a nonrandom process. In this sense, similarity between members of the couple can be relevant for the beginning of the relationship (i.e., assortative mating) and maintenance, being similarity in attractiveness one of the most interesting aspects of this phenomenon. Despite that similarity in attractive traits has been documented, there is a lack of studies including modern morphological measures like fluctuating facial asymmetry or body fat percentage when assessing the effect that similarity in attractiveness could provoke on behaviors and feelings necessary to maintain a long-term relationship (e.g., satisfaction and trust). We assessed the presence of similarity in attractiveness for self-perceived measures (attractiveness and mate value) and physical traits (body fat percentage, body mass index, and fluctuating facial asymmetry) in a population of 196 heterosexual young couples from Chile (n = 392). Then, using actor-partner interdependence models (APIM), we assessed whether satisfaction and trust within the couples were influenced by attractiveness. Our results indicated the presence of similarity for all studied traits with the exception of fluctuating facial asymmetry. In addition, we only found that self-assessment of attractiveness is important for satisfaction in women, and partner's physical attractiveness is important for satisfaction and trust in men. Our results suggest that similarity in attractiveness is not playing a major role in affecting relationship. It is probably that similarity could be better explained from the initial stages of relationship, where the mating market forces conduce to the conformation of similar couples.

On a pedestal: High heels and the perceived attractiveness and evolutionary fitness of women

We analyzed the responses of 448 participants who completed questions on attractiveness and other evolutionary fitness related traits, and long- and short-term mating potential, of a woman in either high heeled or flat shoes. We hypothesized that the woman in high heels would be rated as more attractive and evolutionarily fit by both men and women, and preferred for short-term mating by men. The hypothesis was partially supported. The woman in high heels was perceived as being more sexually attractive, physically attractive, feminine, and of a higher status. Additionally, women rated women as having a higher status regardless of the shoe, than men, while men rated women as having higher short- and long-term mating potential, than women did, regardless of the shoe. We discuss the implications of these findings.

The Effects of Ozone on Visual Attraction Traits of Erodium paularense (Geraniaceae) Flowers: Modelled Perception by Insect Pollinators.

Ozone (O3) effects on the visual attraction traits (color, perception and area) of petals are described for Erodium paularense, an endangered plant species. Plants were exposed to three O3 treatments: charcoal-filtered air (CFA), ambient (NFA) and ambient + 40 nL L−1 O3 (FU+) in open-top chambers. Changes in color were measured by spectral reflectance, from which the anthocyanin reflectance index (ARI) was calculated. Petal spectral reflectance was mapped onto color spaces of bees, flies and butterflies for studying color changes as perceived by different pollinator guilds. Ozone-induced increases in petal reflectance and a rise in ARI under NFA were observed. Ambient O3 levels also induced a partial change in the color perception of flies, with the number of petals seen as blue increasing to 53% compared to only 24% in CFA. Butterflies also showed the ability to partially perceive petal color changes, differentiating some CFA petals from NFA and FU+ petals through changes in the excitation of the UV photoreceptor. Importantly, O3 reduced petal area by 19.8 and 25% in NFA and FU+ relative to CFA, respectively. In sensitive species O3 may affect visual attraction traits important for pollination, and spectral reflectance is proposed as a novel method for studying O3 effects on flower color.

A novel twisted bud mutant from Ziziphus jujubaMill. ‘Dongzao’

The twisted growth of organs is a distinctive and attractive trait for woody plants. A bud mutant with twisted branches was discovered from ‘Dongzao’, the most popular and tasty table cultivar of Chinese jujube (Ziziphus jujuba Mill.). This study explored the morphological and nutritional characteristics and SSR marker changes of the novel variant. Compared to its mother plant and an external control ‘Dongzao’, the variant had shorter fruit (oblate rather than round) and shorter bearing shoots with smaller internode length. Although the sugar/acid ratio increased in the variant fruit, the soluble sugar, titrated acid and ascorbic acid contents of the fruit were not significantly different between the variant and its mother. No differences in the DNA fingerprint were observed between the variant and its mother using 50 pairs of SSR primers. The novel twisted variant of ‘Dongzao’ could be a valuable germplasm for jujube breeding because it has high-quality fruit and good yield. It also has great potential for direct economic utilization and could be used as a desirable material for research on the mechanism of twisted growth.

Genome Sequence of Elaeagnus mollis, the First Chromosome-Level Genome of the Family Elaeagnaceae.

Elaeagnus mollis Diels (Elaeagnaceae) is a species of shrubs and/or dwarf trees that produces highly nutritious nuts with abundant oil and pharmaceutical properties. It is endemic to China but endangered. Therefore, to facilitate the protection of its genetic resources and the development of its commercially attractive traits we generated a high-quality genome of E. mollis. The contig version of the genome (630.96 Mb long) was assembled into 14 chromosomes using Hi-C data, with contig and scaffold N50 values of 18.40 and 38.86 Mb, respectively. Further analyses identified 397.49 Mb (63.0%) of repetitive sequences and 27,130 protein-coding genes, of which 26,725 (98.5%) were functionally annotated. Benchmarking Universal Single-Copy Ortholog assessment indicated that 98.0% of highly conserved plant genes are completely present in the genome. This is the first reference genome for any species of Elaeagnaceae and should greatly facilitate future efforts to conserve, utilize, and elucidate the evolution of this endangered endemic species.

Gender-Responsive Participatory Variety Selection in Kenya: Implications for Common Bean (Phaseolus vulgaris L.) Breeding in Kenya

Participatory variety selection (PVS) is the selection of new varieties among fixed lines by farmers under different target environments. It is increasingly being used to select and promote new crop breeding materials in most African countries. A gender-responsive PVS tool was piloted in Embu and Nakuru in the first and second cropping seasons of 2019 to understand similarities and differences between men’s and women’s varietal and trait preferences for biofortified released varieties and local bean varieties (landraces). Pooled results indicate that varietal and trait preferences between men and women farmers were slightly different but followed gendered roles and division of labor. Women farmers have a higher preference for landraces compared to men due to their availability, affordability and accessibility. Preferences for bean varieties differed between men and women across the two counties. High yielding was the most prioritized trait by both men and women for Mwitemania, Nyota, and Angaza. The findings support the long-held assumption that men prefer market-oriented traits, but women have a greater range of concerns thus less market oriented than men. For Nyota, men’s preferences were shaped by market traits, while women’s preferences were based on the variety being early maturing, resistant to pests and diseases, marketable, and fast cooking. For other varieties, men preferred Mwitemania because it is high yielding, early maturing, resistant to pests and diseases, and marketable. In contrast, women preferred Mwitemania because of seed availability, fast cooking, and early maturing. However, men’s and women’s varietal preferences for Angaza were similar, with them reporting early maturing, resistance to pests and diseases, and marketability as attractive traits. Sociodemographic characteristics such as education, age, marital status, and land ownership underlined the differences in trait preferences. Despite having attractive traits for both men and women, Nyota increased drudgery, displaced women from their usual activities, and required additional inputs by women, signaling possible adoption tradeoffs. However, Nyota, Angaza, and Mwitemania can provide the opportunity to increase employment for women. This study calls for gender integration at the design stage of any breeding system to ensure men and women farmers have access to varieties they prefer for food and income generation.

Toupee or Not Toupee?: Cranial Hair and Perceptions of Men’s Attractiveness, Personality, and Other Evolutionary Relevant Traits

The question of whether or not cranial hair affects perceptions of attractiveness, personality, career success, and other traits related to fitness for men in two populations was investigated in two experiments. Experiment 1 used a 2 (race) × 2 (cranial hair of man) design, and examined attractiveness, fitness, and socially desirable personality measures. Experiment 2 used a 2 (race) × 2 (cranial hair) design to determine perceived attractiveness, fitness-related traits, and the Big-5 dimensions of personality. Amount of cranial hair did not affect personality ratings on the dimensions of the Big-5 but did affect perceived socially desired aspects of personality (such as warmth, sophistication, kindness, etc.). In Experiment 1, the White man with hair received higher perceived attractiveness, personality, and fitness ratings than the bald White man, while no differences occurred for the Black men. For Experiment 2, when differences for amount of cranial hair occurred, the White man with hair and the Black man without hair received higher perceived fitness and career success ratings. These results are discussed in terms of prior research on male cranial hair.

Surgical Versus Nonsurgical Lower Face Contouring: Layperson Perceptions of Attractiveness and Personality Traits.

Traditional approaches to lower face contouring involve ostectomy of the mandibular angle or body. More recently, nonsurgical techniques have gained popularity, including neurotoxin injection to the masseter muscle. This study aims to evaluate layperson perceptions of patient attractiveness and personality traits following lower face contouring using either surgical or nonsurgical treatment. In this survey study distributed via Amazon's Mechanical Turk, respondents viewed a series of 14 patient images obtained in clinic before and after surgical or nonsurgical jawline slimming, or without any facial aesthetic procedure. Respondents rated changes in attractiveness and personality trait scores between the before and after image (score: -50 to 50, with 50 representing the greatest posttreatment increase and 0 representing no change). We used descriptive statistics and multivariable regression to determine differences in respondent ratings between patient images. A total of 415 respondents (mean age 38 years, 50.6% female) successfully completed the survey. Compared to patients who underwent nonsurgical treatment, those who received surgery had significantly greater increases in perceived attractiveness (P < 0.001), femininity (P < 0.001), friendliness (P < 0.001), intelligence (P < 0.001), trustworthiness (P < 0.001), financial wealthiness (P < 0.001), dominance (P < 0.01), and self-esteem (P < 0.001). Gonial angles increased and jaw widths decreased following both surgical and nonsurgical intervention, with no statistically significant difference between treatment groups. From the layperson perspective, surgical compared to nonsurgical jawline contouring offers greater improvements in perceptions of attractiveness and favorable personality traits.

Is a disease leader attractive? Six tests of whether the COVID-19 pandemic affected follower preferences for attractiveness, health and other traits in political and non-political leaders

Attractive political candidates receive more votes on Election Day compared to their less attractive competitors. One well-cited theoretical account for this attractiveness effect (White et al., 2013) holds that it reflects an adaptive psychological response to disease threats. Voters are predicted to upregulate preferences for attractiveness because it constitutes a cue to health. The global COVID-19 pandemic constitutes an ecologically relevant and realistic setting for further testing this prediction. Here, we report the results from six tests of the prediction based on two large and nationally representative surveys conducted in Denmark (n = 3297) at the outbreak of the pandemic and one year later. Utilizing experimental techniques, validated individual difference measures of perceived disease threat and geographic data on COVID-19 severity, we do not find that disease threats like the COVID-19 pandemic upregulate preferences for attractive and healthy political or non-political leaders. Instead, respondents display heightened preferences for health in socially proximate relations (i.e. colleagues). Moreover, individuals who react aversively to situations involving risks of pathogen transmission (scoring high in Germ Aversion) report higher importance of a wide range of leadership traits, rather than for health and attractiveness in particular. Results are discussed in relation to evolutionary accounts of leadership and followership.

Can Conflicting Selection from Pollinators and Nectar-Robbing Antagonists Drive Adaptive Pollen Limitation? A Conceptual Model and Empirical Test.

AbstractPollen limitation is widespread, despite predictions that it should not be. We propose a novel mechanism generating pollen limitation: conflicting selection by pollinators and antagonists on pollinator attraction traits. We introduce a heuristic model demonstrating antagonist-induced adaptive pollen limitation and present a field study illustrating its occurrence in a wild population. For antagonist-induced adaptive pollen limitation to occur, four criteria must be met: (1) correlated attraction of pollinators and antagonists; (2) greater response by antagonists than pollinators to altered investment in attraction traits; (3) reduced investment in pollinator attraction, leading to pollen limitation; and (4) higher fitness for plants with reduced investment in pollinator attraction. We surveyed nectar robbery and reproductive output for 109 Odontonema cuspidatum (Acanthaceae) plants in a pollen-limited population over 2 years and used experimental floral arrays to evaluate how flower number affects pollination and nectar robbery. Both pollinators and nectar robbers preferred larger floral displays and nectar robbery reduced reproductive output, suggesting conflicting selection. Survey and experimental data agreed closely on the optimum flower number under antagonist-induced pollen limitation; this number was substantially overrepresented in the population. While criteria for antagonist-induced adaptive pollen limitation are restrictive, the necessary conditions may often be realized. Considering interactions beyond the plant-pollinator dyad illuminates previously overlooked mechanisms generating pollen limitation.

A sit-and-wait predator, but not an active-pursuit predator, alters pollinator-mediated selection on floral traits.

Indirect species interactions are ubiquitous in nature, often outnumbering direct species interactions. Yet despite evidence that indirect interactions have strong ecological effects, relatively little is known about whether they can shape adaptive evolution by altering the strength and/or direction of natural selection. We tested whether indirect interactions affect the strength and direction of pollinator-mediated selection on floral traits of the bumble-bee pollinated wildflower Lobelia siphilitica. We estimated the indirect effects of two pollinator predators with contrasting hunting modes: dragonflies (Aeshnidae and Corduliidae) and ambush bugs (Phymata americana, Reduviidae). Because dragonflies are active pursuit predators, we hypothesized that they would strengthen pollinator-mediated selection by weakening plant-pollinator interactions (i.e., a density-mediated indirect effect). In contrast, because ambush bugs are sit-and-wait predators, we hypothesized that they would weaken or reverse the direction of pollinator-mediated selection by altering pollinator foraging behavior (i.e., a trait-mediated indirect effect). Specifically, if ambush bugs hunt from plants with traits that attract pollinators (i.e., prey), then pollinators will spend less time visiting those plants, weakening or reversing the direction of selection on attractive floral traits. We did not find evidence that high dragonfly abundance strengthened selection on floral traits via a density-mediated indirect effect: neither pollen limitation (a proxy for the strength of plant-pollinator interactions) nor directional selection on floral traits of L. siphilitica differed significantly between high- and low-dragonfly abundance treatments. In contrast, we did find evidence that ambush bug presence affected selection on floral traits via a trait-mediated indirect effect: ambush bugs hunted from L. siphilitica plants with larger daily floral displays, reversing the direction of pollinator-mediated selection on daily display size. These results suggest that indirect species interactions have the potential to shape adaptive evolution by altering natural selection.

A hybrid cooling system to enable adhesion-free heat recovery from centrifugal granulated slag particles

It remains a long-standing challenge to recover high-temperature heat from blast furnace slag. Centrifugal granulation assisted thermal energy recovery (CGATER), although being proposed for tens of years, has gained renewed interests for its potential to enable green and sustainable slag treatment. Yet, the CGATER technology is still not sufficiently mature to be applied in iron and steel industry. One key hurdle towards its industrialization is the easy adhesion of granulated slag particles, which deteriorates heat removal from slag particles. To address this issue, a new method that combines both water and air cooling to eliminate particles adhesion in the course of heat recovery was proposed. As a proof of principle, a hybrid cooling system was built and the behavior of the hot, sticky slag particles inside the system was studied experimentally. Encouragingly, this hybrid cooling system was observed with superb anti-adhesion performance. Such attractive trait of the new system is enabled by the fast slag particles cooling by water and timely slag particles transportation by air. Further, the operational factors including initial slag temperature, air flowrate and rotating speed of granulator were also considered. The optimal rotating speed of granulator was 1800 rpm in the present operating conditions. The maximum heat recovery efficiency of 52% and vitreous content of ~ 98% were achieved when the initial slag temperature was 1500 ℃. This work provides a new attempt to resolve the cumbersome slag adhesion issue and advances the state of the art in the realm of CGATER technology.

The function of floral traits and phenotypic selection in Aconitum gymnandrum (Ranunculaceae).

Floral evolution in angiosperms is thought to be driven by pollinator-mediated selection. Understanding flower integration and adaptation requires resolving the additive and nonadditive contributions of floral pollinator attraction and pollination efficiency traits to fitness components. In this study, a flower manipulation experiment with a factorial design was used to study the adaptive significance of galea height (a putative attraction trait) and entrance width (a putative efficiency trait) in Aconitum gymnandrum Maxim. flowers. Simultaneously, phenotypic selection analysis was conducted to examine selection by pollinators on galea height, entrance width, nectar production and plant height. Increased galea height increased the pollinator visitation rate, which confirmed its attractiveness function. Increasing floral entrance width by spreading the lower sepals increased the seed number per fruit without affecting pollinator visitation. This suggests a pollination efficiency role for the entrance width. The phenotypic selection analysis, however, did not provide evidence of pollinator-mediated selection for either of these traist, but it did for plant height. According to the manipulation treatment and correlational selection results, the combined variation in galea height and entrance width of A. gymnandrum flowers did not have nonadditive effects on female reproductive success. This study demonstrated the adaptive value of A. gymnandrum flowers through manipulation of an attractiveness trait and an efficiency trait. However, neither trait was associated with pollinator-mediated selection. A combination of manipulating traits and determining current phenotypic selection could help to elucidate the mechanism of selection on floral traits involved in different functions and flower integration.

Interpersonal Attraction Traits on Facebook

Many are using these social media nowadays to enhance the interpersonal relationships, regardless of an existing or a new friend. Facebook users are engaging in numerous ways to initiate or maintain interpersonal relationships, such as increasing the frequency of interactions, improving the quality of communications, and utilizing self-disclosure approaches through displaying interpersonal attraction traits, but do they agree on whether or not to display the traits of interpersonal attraction on Facebook? This study to identify the interpersonal attraction traits, highlighted by Facebook users in order to improve their interaction network through mediated-communication. By using a purposive sampling procedure, a total of 402 respondents who use Facebook, which was invited to fill out a questionnaire on a Google Form. Facebook users agreed that interpersonal attraction traits such as physical attraction, social attraction, extraversion, and popularity, should be highlighted; however, they have to be neutral in highlighting the traits. The findings of this study contribute towards advancing knowledge on reducing uncertainty due to interpersonal attraction traits and to meet the needs for creating a demanding community that facilitate the interactions of people with collective interests for social networking enhancement.

Breeding systems of naturalized versus indigenous species provide support for Baker's law on Pohnpei island.

The factors that facilitate successful colonization of islands should be especially evident where the establishment filter is strongest. Colonizers of small, remote oceanic islands should be initially rare, extremely mate-limited and often without pollinators. Hence, plant communities on such islands should reflect an establishment history in which young ‘naturalized’ species are most likely to display self-compatibility and autonomous selfing, whereas ‘indigenous’ species may exhibit more diverse reproductive strategies. To test this prediction, we characterized breeding systems of 28 species on Pohnpei, in the Federated States of Micronesia, a group of remote Pacific islands that are considered a global biodiversity hotspot. Three families with both naturalized and indigenous species were selected—Fabaceae, Malvaceae and Melastomataceae. Measurements included field observations of dichogamy/herkogamy and floral attraction traits, pollen:ovule (P:O) ratios and experimental hand-pollinations for self-compatibility and pollen limitation. Phylogenetic generalized least squares analyses tested for trait correlations between naturalized and indigenous species. Flowers of all 28 species were bisexual, and pollinator attraction features were common. Pollen:ovule ratios ranged from 9 to 557 (median = 87), and all 11 hand-pollinated species were self-compatible. All species had >5 ovules and <3500 pollen grains per flower. Indigenous species did not differ significantly from naturalized species for any trait. There is a dearth of data from remote islands bearing on the question of establishment history. In this study, we inferred all species to have some degree of autogamy and indigenous species were no more likely than naturalized species to display outcrossing mechanisms. On Pohnpei, high ovule numbers, and the inaccessibility of wind pollination and obligate outcrossing strategies, reflect the importance of retaining reproductive assurance mechanisms in the face of pollinator uncertainty.

The PHYSICOCHEMICAL AND SENSORY CHARACTERISTICS OF PROMISING PLUM (Prunus domestica L.) GENOTYPES BRED AT FRUIT RESEARCH INSTITUTE, ČAČAK

Five plum hybrids (38/62/70, IV/63/81, 32/21/87, 34/41/87 and 22/17/87) and newly released cultivar ‘Nada’, obtained by planned hybridisation and singled out within breeding programme at Fruit Research Institute, Čačak, were assessed for the main physical (fruit and stone weight and flesh percentage), chemical (soluble solids content, total and inverted sugars content, sucrose content, total acids content, pH value of fruit juice, ratio of soluble solids and total acids content and ratio of total sugars and total acids content) and sensorial (attractiveness, taste, aroma and consistency) traits compared with the standard cultivar ʻČačanska Lepoticaʼ. Results showed that the studied plum genotypes differed significantly in all of the assessed traits. Regarding the physical features, the best results were shown by hybrid 38/62/70, which had the highest fruit weight (56.92 g) and flesh percentage (96.91%), as well as by the new cultivar ‘Nada’, for which a larger fruit was observed in comparison to the standard (45.54 g and 42.24 g, respectively). Also, ‘Nada’ had better sensorial properties such as attractiveness, taste, aroma and consistency in relation to the other promising hybrids and the standard cultivar. From the aspect of all the studied sensorial characteristics, in addition to ‘Nada’, only hybrid 38/62/70 was aligned with the standard cultivar. Out of the studied genotypes, late ripening hybrid 22/17/87 had the best values of parameters of fruit chemical composition such as the soluble solids content (17.01%), total and inverted sugars contents (12.31% and 8.96%, respectively). The highest sucrose content (3.39%), pH value of fruit juice (3.51), as well as the highest ratio between soluble solids and total acids content (43.72) and ratio between total sugars and total acids content (32.58) were found in cultivar ‘Nada’. The highest total acids content (1.42%) was recorded in hybrid 32/21/87. Compared to ʻČačanska Lepoticaʼ, the same or better results in terms of the fruit chemical composition were determined in cultivar ‘Nada’ and hybrids 34/41/87 and 22/17/87. The study revealed existence of significant correlations between individual studied parameters of the fruit physical, chemical and sensorial properties.

Transcriptomic Profiling of Apple Calli With a Focus on the Key Genes for ALA-Induced Anthocyanin Accumulation.

The red color is an attractive trait of fruit and determines its market acceptance. 5-Aminolevulinic acid (ALA), an eco-friendly plant growth regulator, has played a universal role in plant secondary metabolism regulation, particularly in flavonoid biosynthesis. It has been widely reported that ALA can up-regulate expression levels of several structural genes related to flavonoid metabolism and anthocyanin accumulation. However, the molecular mechanisms behind ALA-induced expression of these genes are complicated and still far from being completely understood. In this study, transcriptome analysis identified the differentially expressed genes (DEGs) associated with ALA-induced anthocyanin accumulation. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that the flavonoid biosynthesis (ko00941) pathway was significantly enhanced in the ALA-treated apple calli at 24, 48, and 72 h after the treatment. Expression pattern revealed that ALA up-regulated the expression of the structural genes related to not only anthocyanin biosynthesis (MdCHS, MdCHI, MdF3’H, MdDFR, MdANS, and MdUFGT) but also anthocyanin transport (MdGST and MdMATE). Two R2R3-MYB transcription factors (MdMYB10 and MdMYB9), which are the known positive regulators of anthocyanin biosynthesis, were significantly induced by ALA. Gene overexpression and RNA interference assays demonstrated that MdMYB10 and MdMYB9 were involved in ALA-induced anthocyanin biosynthesis. Moreover, MdMYB10 and MdMYB9 might positively regulate the transcription of MdMATE8 by binding to the promoter region. These results indicate that MdMYB10 and MdMYB9 modulated structural gene expression of anthocyanin biosynthesis and transport in response to ALA-mediated apple calli coloration at the transcript level. We herein provide new details regarding transcriptional regulation of ALA-induced color development.

Factors affecting fruit and seed production of Paeonia ostii “Feng Dan”, an economically important oil tree

AbstractMany factors may affect reproduction of animal‐pollinated species. In this study, the effects of pollen limitation, attractive traits (flower number, plant height and flower width) and flowering phenological traits (flowering onset, duration and synchrony) on female reproduction, as well as the patterns of variation in fruit and seed production within plants, were investigated in Paeonia ostii “Feng Dan” over two flowering seasons (2018 and 2019). Fruit set was very high (90%), and pollen supplementation did not increase fruit and seed production in either year, indicating no pollen limitation. Fruit set, ovule number per fruit and mean individual seed weight per fruit were not affected by any of the six attractive and phenological traits in either year, whereas seed number per fruit was related to the three attractive traits in one or both years. Seed number per plant was positively affected by the three attractive traits and best explained by flower number in both years, but the effect of each of the three phenological traits on seed number per plant differed between years. Within plants, the fruit set, ovule number, seed set and seed number per fruit declined from early‐ to late‐opening flowers, presumably because of resource preemption, but the mean individual seed weight did not vary across the flowering sequence. Our study shows that attractive traits of Paeonia ostii “Feng Dan” are more important than flowering phenological traits in the prediction of total seed production per plant.

Attractive males are cautious and better learners in the sailfin tetra

A major component of Darwin's formulation of sexual selection theory is whether mate choice can contribute to the evolution of cognitive abilities. Although a correlation between cognition and attractive traits has commonly been reported, the processes that generate such associations remain elusive. Here, we investigated female preference, neophobia and spatial learning in the sailfin tetra, Crenuchus spilurus , a sexually dimorphic fish. Females chose the most ornamented males, which were also more neophobic. This is in line with the asset protection principle, which posits that attractive males should be more cautious because of their higher prospective fitness returns. After repeated exposure, more ornamented males learnt to navigate the complex maze faster, with chosen (and highest ornamented) males showing higher accuracy to solve the maze on the last day of trials. Because the asset protection principle stems from predation pressure, we propose that sexual selection through female mate choice creates a risk–reward trade-off, upon which natural selection acts by indirectly increasing learning abilities. As such, sexual selection forms the substrate, but natural selection drives enhanced cognitive abilities in attractive C. spilurus males. • Can mate choice favour increased cognition? If so, how? • In the sailfin tetra, females prefer males with larger conspicuous ornaments. • Chosen males were more cautious to avoid predation and took longer in maze trials. • Chosen and conspicuously ornamented males performed better in a spatial learning task. • Mate choice and predation work in concert in the evolution of spatial cognition.