Building insulation upgrades focusing on energy conservation must also consider the hygrothermal performance of materials and the indoor environment. Previous research has shown that deep insulation retrofit may inadvertently reduce ventilation and increase relative humidity, increasing the risk of mould growth. This study used traditional housing in Ireland with pitched roofs as the case study to examine changes in the hygrothermal environment following the energy retrofit of a dormer-style attic room. The study compared the mould growth index (MGI) of roof rafters under different energy retrofit scenarios to understand the effect of adding a habitable dormer-style attic room on the house's mould growth risk. The results show that adding an occupied dormer room can increase the MGI of spruce rafters from below 1 to around 5.2 at 0.2 air changes per hour (ACH) of infiltration. In most scenarios, a ventilation rate lower than 1 ACH cannot prevent the MGI of rafters from reaching the critical threshold of 3, unless a 1 mm vapour barrier is added. A ventilation rate over 5 ACH is adequate to limit the mould growth risk, but mechanical ventilation may be required to achieve this level of attic ventilation. This study enhances understanding of the relationship between attic roof ventilation rate and the risk of mould growth during the energy upgrade of dormer style attic rooms, a common housing type across Northern Europe and further afield.