Intensity Attenuation Research Articles

Label-free dual-mode sensing platform based on target-regulated CRISPR-Cas12a activity for ochratoxin A in Morinda officinalis.

Many traditional Chinese herbs are susceptible to ochratoxin A (OTA), a potent mycotoxin, which causes serious effects on the quality of the herb and on people's health. The development of methods to detect OTA is extremely important. Most methods for detecting OTA are based on a single-signal output mode, which might be easily influenced by complex environmental conditions. In this research, by taking advantage of the cleavage of DNA by target-induced CRISPR-Cas12a activity and the difference in electrostatic force of DNA to different charge electrochemiluminescent (ECL) and electrochemical (EC) probes, a biosensor is developed for the detection of OTA. First, the CRISPR-Cas12a system consists of a well-designed crRNA, its complementary strand (also as an aptamer for OTA), and Cas12a. Without the target, this CRISPR-Cas12a system is in the "activated stage", which digests hairpin DNA on the electrode, resulting in a weak ECL signal and strong current response. With the introduction of OTA bound with the aptamer, CRISPR-Cas12a activity is inhibited ("locked stage"). Thus, hairpin DNA remained intact on the electrode, resulting in recovery of the ECL signal and attenuation of the current intensity. As a result, this label-free dual-mode sensing platform realizes an assay for OTA in Morinda officinalis. This target-regulated CRISPR-Cas12a activity-sensing platform with dual-mode output not only provides high sensitivity (due to the CRISPR-Cas12a system), but also has good anti-interference ability against complex substrates (due to dual-mode output), and exhibits a broad range of prospects for application.

Quantitative Imaging of Temperature and OH Concentration From Single Wavelength Planar Laser-Induced Fluorescence

A planar laser induced fluorescence (PLIF) of single wavelength is introduced to image temperature and concentration distribution inside flames. Laser absorption spectroscopy (LAS) tomography was used to provide initial images of gas temperature for image iteration. The images of temperature and concentration are used to update the fluorescence image. The fluorescence images are also corrected to update images of temperature and radical concentrations iteratively. The intensity attenuation losses due to absorption in PLIF is corrected in the iterations. Single wavelength PLIF and LAS tomography are simultaneously performed, and fluorescence image at a single wavelength is required only for each quantitative measurement. The proposed method has a twice higher speed than the classical dual wavelength PLIF. In numerical simulations, the relative error of reconstructed images of temperature is within 7 % and that of the concentration is less than 3 % with a signal-to-noise ratio higher than 30 dB. Experiments were conducted on a McKenna burner, quantitative images of temperature and OH concentration were obtained and compared with CFD, thermocouple and two-color PLIF. The absolute errors are less than 30 K in the center of the burner. The relative errors of concentration are less than 7 % in the flat flames.

Research on propagation characteristic of Laguerre–Gaussian vortex beams with Far-field scattering Electric Monte Carlo method

A Far-field Scattering Electric field Monte Carlo (FSEMC) method is proposed to investigate the propagation and imaging characteristics of Laguerre–Gaussian Beams (LGB) with different Orbital Angular Momentum (OAM) in long-distance (relative to wavelength) scattering environment. As an extension of the Electric field Monte Carlo (EMC) method, the electric field transport mode is simulated to properly analyze the fading effect of scattering. The mean free path and total transmission length in the simulation are equivalent to the environment parameters of experiment. The beam transmission modes, intensity attenuation and effects on imaging quality through different turbidity media are analyzed. It is observed that vortex beams with different topological charges play a subtle role in transmission through turbid media, showing strong correlations with total transmission intensity, retention of ballistic light, and penetration depth. The experimental results fitting well with the FSEMC simulation results, validating the proposed method for far-field propagation characteristic of vortex beams in turbid media.

Water irradiation devoid pulses enhance the sensitivity of 1H,1H nuclear Overhauser effects.

The nuclear Overhauser effect (NOE) is one of NMR spectroscopy's most important and versatile parameters. NOE is routinely utilized to determine the structures of medium-to-large size biomolecules and characterize protein-protein, protein-RNA, protein-DNA, and protein-ligand interactions in aqueous solutions. Typical [1H,1H] NOESY pulse sequences incorporate water suppression schemes to reduce the water signal that dominates 1H-detected spectra and minimize NOE intensity losses due to unwanted polarization exchange between water and labile protons. However, at high- and ultra-high magnetic fields, the excitation of the water signal during the execution of the NOESY pulse sequences may cause significant attenuation of NOE cross-peak intensities. Using an evolutionary algorithm coupled with artificial intelligence, we recently designed high-fidelity pulses [Water irrAdiation DEvoid (WADE) pulses] that elude water excitation and irradiate broader bandwidths relative to commonly used pulses. Here, we demonstrate that WADE pulses, implemented into the 2D [1H,1H] NOESY experiments, increase the intensity of the NOE cross-peaks for labile and, to a lesser extent, non-exchangeable protons. We applied the new 2D [1H,1H] WADE-NOESY pulse sequence to two well-folded, medium-size proteins, i.e., the K48C mutant of ubiquitin and the Raf kinase inhibitor protein. We observed a net increase of the NOE intensities varying from 30 to 170% compared to the commonly used NOESY experiments. The new WADE pulses can be easily engineered into 2D and 3D homo- and hetero-nuclear NOESY pulse sequences to boost their sensitivity.

Multi‐Dimensional Structure and Dynamics Landscape of Proteins in Mammalian Cells Revealed by In‐Cell NMR

AbstractGoverning function, half‐life and subcellular localization, the 3D structure and dynamics of proteins are in nature constantly changing in a tightly regulated manner to fulfill the physiological and adaptive requirements of the cells. To find evidence for this hypothesis, we applied in‐cell NMR to three folded model proteins and propose that the splitting of cross peaks constitutes an atomic fingerprint of distinct structural states that arise from multiple target binding co‐existing inside mammalian cells. These structural states change upon protein loss of function or subcellular localisation into distinct cell compartments. In addition to peak splitting, we observed NMR signal intensity attenuations indicative of transient interactions with other molecules and dynamics on the microsecond to millisecond time scale.

Multi-Dimensional Structure and Dynamics Landscape of Proteins in Mammalian Cells Revealed by In-Cell NMR.

Governing function, half-life and subcellular localization, the 3D structure and dynamics of proteins are in nature constantly changing in a tightly regulated manner to fulfill the physiological and adaptive requirements of the cells. To find evidence for this hypothesis, we applied in-cell NMR to three folded model proteins and propose that the splitting of cross peaks constitutes an atomic fingerprint of distinct structural states that arise from multiple target binding co-existing inside mammalian cells. These structural states change upon protein loss of function or subcellular localisation into distinct cell compartments. In addition to peak splitting, we observed NMR signal intensity attenuations indicative of transient interactions with other molecules and dynamics on the microsecond to millisecond time scale.

Non-invasive estimation of coral polyp volume and surface area using optical coherence tomography

The surface area (SA) and three-dimensional (3D) morphology of reef-building corals are central to their physiology. A challenge for the estimation of coral SA has been to meet the required spatial resolution as well as the capability to preserve the soft tissue in its native state during measurements. Optical Coherence Tomography (OCT) has been used to quantify the 3D microstructure of coral tissues and skeletons with nearly micron-scale resolution. Here, we develop a non-invasive method to quantify surface area and volume of single coral polyps. A coral fragment with several coral polyps as well as calibration targets of known areal extent are scanned with an OCT system. This produces a 3D matrix of optical backscatter that is analyzed with computer algorithms to detect refractive index mismatches between physical boundaries between the coral and the immersed water. The algorithms make use of a normalization of the depth dependent scatter intensity and signal attenuation as well as region filling to depict the interface between the coral soft tissue and the water. Feasibility of results is judged by inspection as well as by applying algorithms to hard spheres and fish eggs whose volume and SA can be estimated analytically. The method produces surface area estimates in calibrated targets that are consistent with analytic estimates within 93%. The appearance of the coral polyp surfaces is consistent with visual inspection that permits standard programs to visualize both point clouds and 3-D meshes. The method produces the 3-D definition of coral tissue and skeleton at a resolution close to 10 µm, enabling robust quantification of polyp volume to surface area ratios.

An integrated system for user-friendly X-ray intensity attenuation in a wide energy range

For undulator hard X-ray beamlines of a wide energy range, the beam intensity control is often needed to regulate the photon flux impinging on delicate detectors. Despite the capability of state-of-the-art X-ray pixel detectors being greatly advanced recently, the frontier undulator beamlines in synchrotron facilities often carry outstanding photon fluxes that are sometimes too high for certain measurements. Here, we report a developed protocol that allows intensity attenuation of an X-ray beam in the 4-23 keV energy range with flexible attenuation factors; a prototype is installed and tested on the 13A biological small-angle X-ray scattering beamline of the 3 GeV Taiwan Photon Source (TPS) of the National Synchrotron Radiation Research Center, Hsinchu, Taiwan. The intensity attenuation system is modified from a commercially available pneumatically actuated, vacuum-type precision X-ray attenuator of ADC ABS-300; the system provides beam attenuation factors covering 8 orders of intensity attenuation for an X-ray beam in 4-23 keV. This was achieved with selected combinations of 10 sets of metal foils comprising different thicknesses of Al, Ti, Cu, and Ta foils. A user-friendly protocol is established to automatically compare a subscribed attenuation factor with all the possible attenuation factors from the 1024 combinations of the 10 sets of foils in the X-ray energy used, and determine a set of the metal foils having a best-matched attenuation factor. Calculation of beam intensity attenuations with the selected foils is coded with Python and integrated into the Experimental Physics and Industrial Control System (EPICS). The input of an attenuation factor is done through a graphical display of the attenuator system based on the Control System Studio (CSS). The developed X-ray beam attenuation system provides a convenient and intuitive beam intensity control and has the potential to be adopted in future beamlines.

The European Preinstrumental Earthquake Catalogue EPICA, the 1000–1899 catalogue for the European Seismic Hazard Model 2020

Abstract. The European PreInstrumental Earthquake CAtalogue (EPICA) (Rovida and Antonucci, 2021; https://doi.org/10.13127/epica.1.1) is the 1000–1899 seismic catalogue compiled for the European Seismic Hazard Model 2020 (ESHM20), an outcome of the project Seismology and Earthquake Engineering Research Infrastructure Alliance for Europe (SERA), in the framework of the European Union's Horizon 2020 research and innovation programme. EPICA is the update of the SHARE European Earthquake Catalogue (SHEEC) 1000–1899, with which it shares the main principles – mostly transparency, repeatability and continent-wide harmonisation of data – as well as the compilation strategies and methods. Version 1.1 of EPICA contains 5703 earthquakes with either maximum intensity ≥5 or Mw≥4.0, with a spatial coverage from the Atlantic Ocean to the west to 32∘ E in longitude, and from the Mediterranean Sea to Northern Europe. EPICA relies upon the updated knowledge of the European preinstrumental seismicity provided by the data gathered in the European Archive of Historical Earthquake Data (AHEAD). Such data are both macroseismic intensity data supplied by descriptive historical seismological studies and online macroseismic databases, and parameters contained in regional catalogues. As done for the compilation of SHEEC 1000–1899, these datasets were thoroughly analysed in order to select the most representative of the knowledge of each earthquake, independently from national constraints. Selected intensity distributions are processed with three methods to determine location and magnitude based on the attenuation of macroseismic intensity and are combined with parameters harmonised from modern regional catalogues. This paper describes the compilation procedure of EPICA version 1.1, its input data, the assessment of the earthquake parameters and the resulting catalogue, which is finally compared with its previous version. Technical solutions for accessing the catalogue, both as downloadable files and through web services, are also illustrated.

Accurate correction method and algorithm of fluorescence secondary inner filter effect (sIEF) in fluorescence quantitative analysis

Fluorescence spectroscopy is a reliable and widely used analytical method. The fluorescence inner filter effect (IFE) is one of the main obstacles in the application of fluorescence spectroscopy and an error source in fluorescence analysis, resulting in the fluorescence spectrum distortion, the spectral shape distortion, and the nonlinearity between fluorescence intensity and fluorophore concentration. An optimized parameter reflecting the self-absorption effect - the fluorescence attenuation absorption index of secondary inner filter effect (sIFE) nopt - is proposed in this paper. Considering the received fluorescence in a direction perpendicular to the incident light, it is related to the solute–solvent system of the fluorescent substance, neither the geometric parameters of the cuvette and the light beam nor the concentration of the fluorescent substance. nopt can accurately reflect the degree to which the fluorescence is affected by the sIFE and correct for any non-ideality of the shapes of excitation/emission beams. The principle and determination method of nopt are explained in detail. Accordingly, an algorithm for the fluorescence spectroscopic correction by nopt is designed. To verify the method, the fluorescence spectra and absorbance spectra of the solutions of fluorescein sodium, rhodamine B, rhodamine 6G, and chlorophyll-a with a series of concentration gradients were measured, respectively. The influence of solvent effect on sIFE correction was also studied. The experiments show that different solute–solvent systems of the fluorescent substances have their own nopt. The novel algorithm can determine the nopt, correct the intensity attenuation and the peak red-shift of the fluorescence spectrum caused by the sIFE, expand the linear range of the concentration predicted by the fluorescence intensity, reduce the error of the prediction model, and improve the measurement accuracy.

Temperature-Dependent Photoluminescence of CdS/ZnS Core/Shell Quantum Dots for Temperature Sensors.

Exploring the temperature-dependent photoluminescence (PL) properties of quantum dots (QDs) is not only important for understanding the carrier recombination processes in QD-based devices but also critical for expanding their special applications at different temperatures. However, there is still no clear understanding of the optical properties of CdS/ZnS core/shell QDs as a function of temperature. Herein, the temperature-dependent PL spectra of CdS/ZnS core/shell QDs were studied in the temperature range of 77-297 K. It was found that the band-edge emission (BEE) intensity decreases continuously with increasing temperature, while the surface-state emission (SSE) intensity first increases and then decreases. For BEE intensity, in the low temperature range, a small activation energy (29.5 meV) in the nonradiative recombination process led to the decrease of PL intensity of CdS/ZnS core/shell QDs; and at high temperature the PL intensity attenuation was caused by the thermal escape process. On the other hand, the temperature-dependent variation trend of the SSE intensity was determined by the competition of the trapping process of the surface trap states and the effect of thermally activated non-radiative defects. As the temperature increased, the PL spectra showed a certain degree of redshift in the peak energies of both band-edge and surface states and the PL spectrum full width at half-maximum (FWHM) increases, which was mainly due to the coupling of exciton and acoustic phonon. Furthermore, the CIE chromaticity coordinates turned from (0.190, 0.102) to (0.302, 0.194), which changed dramatically with temperature. The results indicated that the CdS/ZnS core/shell QDs are expected to be applied in temperature sensors.

Comprehensive modeling and predicting light transmission in microalgal biofilm

Biofilm-based microalgae culture combined with wastewater treatment is a promising biotechnology for environmental management. Light availability influences the accumulation of microalgal biomass and nutrient removal. A light attenuation model which comprehensively considered microalgal biofilm structure (density and biofilm thickness), pigments content, and extracellular polymeric substances content was developed to predict the light attenuation in biofilm according to the simplification of the radiative transfer equation. The predicted results were in good overall agreement with the experiment, with an average error of less than 9.02%. These factors (biofilm density, thickness, pigments content, and extracellular polymeric substances content) all contributed to the light intensity attenuation, but biofilm thickness caused the most dramatic attenuation under the same increment of relative change in actual culture. The scattering coefficient of the biofilm (0.433 m2/g) was less than that of the suspension (1.489 m2/g) under white incident light. It suggests that the dense structure of cells allows much light to be concentrated in the forward direction when transmitting. This model could be adopted to predict the light distribution in microalgal biofilm for the further design of efficient photobioreactors and the development of light optimization strategies.

A comprehensive assessment of four whole blood stabilizers for flow-cytometric analysis of leukocyte populations.

Though cryopreservation of cell fractions is widely used in flow cytometry studies, whole blood cryopreservation is more challenging due to the presence of erythrocytes and effects of fixatives commonly used for preservation. Here, we evaluated and compared head-to-head the performance of four commercial whole blood cryopreservation kits; (1) Cytodelics, (2) Stable-Lyse V2 and Stable-Store V2 (SLSS-V2), (3) Proteomic stabilizer (PROT-1), and (4) Transfix. We found that PROT-1, Transfix, and Cytodelics maintained the distribution of major leukocyte subsets-granulocytes, T cells, natural killer cells, and B cells, on a comparable level to unpreserved samples, despite the attenuation of fluorescence intensities in flow cytometric assays. Moreover, these three stabilizers also maintained the activated phenotypes of neutrophils upon stimulation with N-formylmethionyl-leucyl-phenylalanine and lipopolysaccharides. The upregulation of adhesion molecules (CD11b), Fc receptors (CD16), and granule proteins (CD66b), as well as the shedding of surface L-selectin (CD62L), was conserved most efficiently in PROT-1 and Cytodelics when compared to samples only treated with erythrocyte lysing. However, none of the stabilizers provided a reliable detection of CCR7 for accurate quantification of T cell maturation stages. We also evaluated the performance of Cytodelics in longitudinal clinical samples obtained from acute COVID-19 patients, where it allowed reliable detection of lymphopenia and granulocyte expansion. These results support the feasibility of whole blood cryopreservation for immunophenotyping by flow cytometry, particularly in longitudinal studies. In conclusion, the performance of different stabilizers is variable and therefore the choice of stabilizers should depend on cell type of interest, as well as antibody clones and experimental design of each study.

Analysis of Hydraulic Characteristics According to the Cross-Section Changes in Submerged Rigid Vegetation

<i>Recently, not only Korea but also the world has been suffering from problems related to coastal erosion. The hard defense method has been primarily used as a countermeasure against erosion. However, this method is expensive and has environmental implications. Hence, interest in other alternative methods, such as the eco-friendly vegetation method, is increasing. In this study, we aim to analyze the hydraulic characteristic of submerged rigid vegetation according to the cross-sectional change through a hydraulic experiment and numerical simulation. From the hydraulic experiment, the reflection coefficient, transmission coefficient, and energy dissipation coefficient were analyzed according to the density, width, and multi-row arrangement of the vegetation zone. From numerical simulations, the flow field, vorticity distribution, turbulence distribution, and wave distribution around the vegetation zone were analyzed according to the crest depth, width, density, and multi-row arrangement distance of the vegetation zone. The hydraulic experiment results suggest that the transmission coefficient decreased as the density and width of the vegetation zone increased, and the multi-row arrangement condition did not affect the hydraulic characteristics significantly. Moreover, the numerical simulations showed that as the crest depth decreased, the width and density of vegetation increased along with vorticity and turbulence intensity, resulting in increased wave height attenuation performance. Additionally, there was no significant difference in vorticity, turbulence intensity, and wave height attenuation performance based on the multi-row arrangement distance. Overall, in the case of submerged rigid vegetation, the wave energy attenuation performance increased as the density and width of the vegetation zone increased and crest depth decreased. However, the multi-row arrangement condition did not affect the wave energy attenuation performance significantly.</i>

S.M.A.R.T. F.U.S: Surrogate Model of Attenuation and Refraction in Transcranial Focused Ultrasound

Low-intensity focused ultrasound (LIFU) is an increasingly applied method for achieving non-invasive brain stimulation. However, transmission of ultrasound through the human skull can substantially affect focal point characteristics of LIFU, including dramatic attenuation in intensity and refraction of focal point location. These effects depend on a high-dimensional parameter space, making these effects difficult to estimate from previous work. Instead, focal point properties of LIFU experiments are often estimated using numerical simulation of LIFU sonication through skull. However, this procedure presents many entry barriers to even computationally savvy investigators and often requires expensive computational hardware, impeding LIFU research. We present a novel MATLAB toolbox (data: doi:10.5068/D1QD60; Matlab Scripts: https://doi.org/10.5281/zenodo.5811122) for rapidly estimating beam properties of LIFU transmitted through bone. Users provide specific values for frequency of LIFU, bone thickness, angle at which LIFU is applied, depth of the LIFU focal point, and diameter of the transducer used and receive an estimation of the degree of refraction/attenuation expected for the given parameters.

Mid-IR dispersion spectroscopy – A new avenue for liquid phase analysis

Mid-IR dispersion spectroscopy is an attractive, novel approach to liquid phase analysis that extends the possibilities of traditional methods based on the detection of absorption via intensity attenuation. This technique detects inherent refractive index changes (phase shifts) induced by IR light interaction with absorbing matter. In contrast to classic absorption spectroscopy, it provides extended dynamic range, baseline-free detection, constant sensitivity, and inherent immunity to power fluctuation. In this paper, we provide a detailed experimental and theoretical characterization and verification of this method with special focus on broadband liquid sample analysis. For this purpose, we develop a compact benchtop dispersion spectroscopy setup based on an EC-QCL coupled to a Mach-Zehnder interferometer. Phase-locked interferometric detection enables to fully harness the advantages of the technique. By instrument operation in the quadrature point combined with balanced detection, the full immunity towards laser power fluctuations and the environmental noise can be achieved. On the example of ethanol (0.5–50% v/v) dissolved in water, it is experimentally demonstrated that changes of the refractive index function are linearly related to concentration also for strongly absorbing, highly concentrated samples beyond the validity of the Beer-Lambert law. Characterization of the sensitivity and noise behavior indicates that the optimum applicable pathlength for liquid analysis can be extended beyond the ones for absorption spectroscopy. Experimental demonstration of the advantages over classical absorption spectroscopy illuminates the potential of dispersion spectroscopy as upcoming robust and sensitive way of recording IR spectra of liquid samples.

Attenuation of turbulence in a periodic cube by finite-size spherical solid particles

To investigate the attenuation of turbulence in a periodic cube due to the addition of spherical solid particles, we conduct direct numerical simulations using an immersed boundary method with resolving flow around each particle. Numerical results with systematically changing particle diameters and Stokes numbers for a fixed volume fraction $\varLambda$ show that the additional energy dissipation rate in the wake of particles determines the degree of the attenuation of turbulent kinetic energy. On the basis of this observation, we propose formulae describing the condition and degree of the attenuation of turbulence intensity. We conclude that particles with size proportional to $\lambda /\sqrt {\gamma }$ , where $\lambda$ and $\gamma$ are the Taylor length and the mass density ratio between particles and fluid, most significantly reduce the intensity of developed turbulence under the condition that $\gamma$ and $\varLambda$ are fixed.

Analysis and comparison of electron radiation damage assessments in Cryo-EM by single particle analysis and micro-crystal electron diffraction.

Electron radiation damage to macromolecules is an inevitable resolution limit factor in all major structural determination applications using cryo-electron microscopy (cryo-EM). Single particle analysis (SPA) and micro-crystal electron diffraction (MicroED) have been employed to assess radiation damage with a variety of protein complexes. Although radiation induced sidechain density loss and resolution decay were observed by both methods, the minimum dose of electron irradiation reducing high-resolution limit reported by SPA is more than ten folds higher than measured by MicroED using the conventional dose concept, and there is a gap between the attained resolutions assessed by these two methods. We compared and analyzed these two approaches side-by-side in detail from several aspects to identify some crucial determinants and to explain this discrepancy. Probability of a high energy electron being inelastically scattered by a macromolecule is proportional to number of layers of the molecules in its transmission path. As a result, the same electron dose could induce much more site-specific damage to macromolecules in 3D protein crystal than single particle samples. Major differences in data collection and processing scheme are the key factors to different levels of sensitivity to radiation damage at high resolution between the two methods. High resolution electron diffraction in MicroED dataset is very sensitive to global damage to 3D protein crystals with low dose accumulation, and its intensity attenuation rates at atomic resolution shell could be applied for estimating ratio of damaged and total selected single particles for SPA. More in-depth systematically radiation damage assessments using SPA and MicroED will benefit all applications of cryo-EM, especially cellular structure analysis by tomography.

Study on laser transmission interference characteristics based on atmospheric turbulent medium

The characteristics of laser transmission interference in the atmospheric turbulent medium are studied in this paper in order to obtain the target interference image with atmosphere as the transmission medium and obtain more accurate detection targets. First, the influence of the laser cross section radius and transmission distance on the laser intensity is analyzed, and thereby, the intensity model of lights transmitted in the turbulent medium is obtained. Through the combination of such model with the ideal reference light, the characteristics of atmospheric turbulent medium-based laser transmission interference are obtained. The influencing factors and the significance degree of influence of interference contrast are analyzed in detail. Studies have shown that the attenuation of laser intensity in the atmospheric turbulent medium is obvious and can be effectively adjusted by increasing the cross section radius. The laser interference contrast is also affected by the cross section radius and transmission distance; of the two, the latter has more obvious influence.

Sensory attenuation from action observation

A central claim of many embodied approaches to cognition is that understanding others' actions is achieved by covertly simulating the observed actions and their consequences in one's own motor system. If such a simulation occurs, it may be accomplished through forward models, a component of the motor system already known to perform simulations of actions and their consequences in order to support sensory-monitoring of one's own actions. Forward-model simulations cause an attenuation of sensory intensity, so if the simulations hypothesized by embodied cognition are indeed provided by forward models, then action observation should trigger this sensory attenuation. To test this hypothesis, the experiments reported here measured the perceived intensity of a touch sensation on the finger when participants observed an active touch (a finger reaching to touch a ball) vs. a passive touch (a ball rolling to touch an unmoving finger). The touch sensation was perceived as less intense during observation of active touch in comparison with observation of passive touch, providing evidence that forward models are indeed engaged during action observation. The strength of this sensory attenuation is compared and contrasted with a well-established sensory-amplification effect caused by visual attention. This sensory-amplification effect has not generally been considered in studies related to sensory attenuation in action observation, which may explain conflicting results reported in the field.