Purpose: Gumiganghwal-tang (known as Kumi-Kyokatsuto in Japanese) is well known traditional herbal prescription, which consists of 12 different herbs; Osterici Radix, Saposhnikoviae Radix, Cnidii Rhizoma, Angelicae Dahuricae Radix, Atractylodis Rhizoma, Scutellariae Radix, Rehmanniae Radix, Asari Radix et Rhizoma, Glycyrrhizae Radix et Rhizoma, Zingiberis Rhizoma Recens, Zizyphi Fructus, and Allii Fistulosi Bulbus. Gumiganghwal-tang has been commonly used in various disease including common cold, pain, and inflammatory diseases in Korea. Montelukast has been used as an effective therapeutic agent for prevention and treatment of allergic rhinitis or asthma. The objective of this study is to find out the combination effect on interaction of Gumiganghwaltang water extract (GGTW) and montelukast using human bronchial epithelial, BEAS-2B cells. Methods:Anti-inflammation effects were selected to study the effects on IL-4/TNF-induced inflammatory response in BEAS2B cells. Cell supernatants and mRNA were collected for cellular inflammatory mediators determined via enzymelinked immunosorbent assay, gelatin zymography, and polymerase chain reaction. Results: After treatment of IL-4/TNF, the production of eotaxin, regulated on activation of normal T-cell-expressedandsecreted (RANTES), matrix metalloproteinases-9 (MMP9), and vascular cell adhesion molecule (VCAM)-1 expression were increased. However, each of GGTW and montelukast was significantly inhibit the production of eotaxin, RANTES, MMP-9, andVCAM-1 expression.Moreover, drug combinations (co-treatment ofGGTWwithmontelukast)weremore effective than each treatment, although the difference was not significant. Conclusion: Taken together, these findings suggest that GGTW combined with montelukast may be useful to reduce airway inflammation, which may explain its beneficial effect for the regulation of inflammatory disease. Contact: Woo-Young Jeon, ssamggun85@kiom.re.kr