ATP-linked Respiration Research Articles

Chronic Ethanol Consumption Increases the Sensitivity of Hepatocyte Mitochondria To NO-Dependent Bioenergetic Dysfunction

Figure 2. Chronic alcohol consumption sensitizes hepatocytes to NO-induced mitochondrial dysfunction. (A) Bioenergetic profile of isolated hepatocytes from control and EtOH-fed rats treated with 500 μM DetaNONOate (D) for 4h followed by subsequent injections of oligomycin (O), FCCP (F), and antimycin-A plus rotenone (A+R). (B) Basal OCR after DetaNONOate injection (C) ATP-linked respiration is equal to the oligomycin-induced decrease in OCR, with the remaining OCR due to proton leak. (D) Maximal OCR was measured following FCCP injection. (E) The reserve capacity was calculated from the difference between the maximal and basal OCR. (F) The non-mitochondrial OCR was determined by injecting antimycin-A plus rotenone simultaneously to fully inhibit the electron transport chain. Results are mean ± SEM. n=5 per group. *p<0.005 compared to Control. #p<0.05 compared to EtOH. $p<0.05 compared to Control + DetaNO.