Atomic Absorption Spectrophotometry Method Research Articles

A GIS-based analysis of heavy metals around Otukpo rice mill in Benue state

Heavy metals are primarily sourced from natural pedo-geochemical backgrounds and anthropogenic occurrences like composting, sewage sludge, aerosol deposition, and waste animal manure. The study aims to determine the heavy metals within the rice mill in Otukpo to understand the spatial distribution with the objectives to examine the heavy metals within the identified activity areas in the Otukpo rice mill, to determine the spatial distribution of heavy metals within the study area and to compare heavy metal values with the acceptable limits. Soil samples were collected from ten locations at Otukpo Rice Mill to analyze heavy metal concentrations. The samples were collected using sterile bottles and a stainless trowel, and the soil samples were analyzed for the concentrations of Cadmium, Copper, Lead, Nickel, Zinc, Mercury, Iron, Arsenic, and Chromium using the Atomic Absorption Spectrophotometry method. The result was analyzed using interpolation to see the variation of the Heavy metals within the various activity areas. The result of the study reveals that Iron has the highest concentration at the Entrance point and lowest at the Generator house with a range value of 3.92 – 1.12mg/kg respectively. The highest concentration of Zinc was recorded at the Mechanic workshop while the lowest is at the Drying point with a range value of 0.135 – 0.872mg/kg respectively. The Husk dumpsite recorded the highest concentration of Copper (0.666mg/kg) with the Drying point recording the least (0.088 mg/kg). The study reveals uneven concentrations and highlights the effectiveness of the Geographic Information System in mapping heavy metal concentrations.

Maternal blood lead level and its impact on cord blood hematological parameters

Introduction. More than 13 annual million deaths are caused by environmental pollutants worldwide. Urbanization, population growth, industrialization and globalization affect our lives both positively and negatively. Women can become lead exposed through occupational and environmental sources. Once lead enters the body, it is mainly deposited in diverse organs: brain, kidneys, liver and bones. The body stores lead mainly in the bones, where it accumulates over time that may be further released into the bloodstream during pregnancy, thus posing a threat to growing fetus.Aim: to examine a lead impact on newborn hematological parameters during perinatal period.Materials and Methods. A retrospective cohort study with 306 pregnant women and paired newborns was carried out. Peripheral blood lead level (BLL) in pregnant and postpartum women was analyzed by using the atomic-absorption spectrophotometry method. Blood specimens were collected for analysis in the third trimester of pregnancy.Results. We have detected a statistically significant decrease in the mean corpuscular hemoglobin concentration (MCHC) in babies born to mothers with BLL > 0.24 µmol/L vs. BLL < 0.24 µmol/L. This difference may indicate a decline in hemoglobin fetal production caused by lead intoxication.Conclusion. Study corroborates an idea that pregnant women with occupational or environmental lead exposure should be monitored for BLL, which should not exceed 0.24 µmol/L during pregnancy.

Analysis Of Krosok Salt Compound Content From The Results Of The Salt Farmers Group In Kersik Village Kutai Kartanegara Regency

Salt is a source of electrolytes and iodine which are very important for the human body. East Kalimantan Province has the potential for diverse coastal and marine resources with high economic value, so it has great potential for optimal, effective, efficient and sustainable management. This research aims to determine the compound content of krosok salt that will be distributed to the public with NSI. This research method is based on NSI 3556:2016 and Atomic Absorption Spectrophotometry (AAS) method, including: (1) Water content analysis; (2) Analysis of the part that is not soluble in water; (3) NaCl content; (4) KIO3 levels; (5) Analysis of metal contamination. This research was conducted on krosok salt in Kersik Village, Kutai Kartanegara Regency. The NaCl salt content does not meet the minimum NSI requirements, namely 94%. Meanwhile, Pb, Cd, metal contamination meets the requirements with values ​​below 10 mg/kg and 0.5 mg/kg.

Pathological Impacts Due to The Existence of Plastic Waste in Rumen of Bali Cattle

This study aimed to determine the prevalence of Bali cattle rumen containing plastic waste and its impact on tissues and health risks. A total of 100 Bali cattle were investigated after being slaughtered at several traditional slaughterhouses in Denpasar City. Rumen samples were examined for any plastic waste, meanwhile blood, liver, kidney, lungs, spleen, intestine, and myocardium were collected for evaluation of heavy metals content and histopathological examination. The heavy metal measurement was performed using the atomic absorption spectrophotometry (AAS) method. Those tissues for histopathological examination were fixed in buffer neutral formalin (BNF) and then evaluated using hematoxylin-eosin (HE) staining. The results of the examination reported 9 rumens (9%) containing plastic waste. The hematological profile showed leucocytosis and the content of lead (0.841 ± 0.522 ppm). Histopathological changes in the liver, kidneys, lungs, spleen, and intestine, were found in various types of degeneration, inflammation, and mild necrosis. In conclusion, the prevalence of plastic waste in the rumen of Bali cattle was 9%, with leucocytosis, lead content was 0.841 ± 0.522 ppm, and various histopathological lesions such as congestion, inflammation, and fatty degeneration in parenchymous tissues.

Metal-accumulating capacity and antioxidant activity of Pylaisia polyantha (Hedw.) Schimp. moss in urban areas

Background. Bryophytes, including mosses, are widely used in biomonitoring of atmospheric pollution due to their ability to accumulate metals from atmospheric air. However, the effects of metal accumulation on metabolic processes in bryophyte cells have not been thoroughly studied. The aim of this work was to analyse the accumulation of heavy metals, indices of lipid peroxidation (LPO), and antioxidant status in gameto­phytes of the epiphytic moss Pylaisia polyantha (Hedw.) Schimp. collected from urban areas with different levels of technogenic load. Materials and Methods. The study was conducted in the city of Lviv (western part of Ukraine). Within the city, 15 sampling sites were selected and grouped as fol­lows: the green zone (A) conditionally used as the control, zone influenced by transport activities (B) and the industrial zone (C). In gametophyte shoots of P. polyantha, con­centrations of heavy metals (Cd, Co, Cr, Cu, Fe, Mn, Ni, Pb, and Zn) were analysed using the method of atomic absorption spectrophotometry; the content of LPO products (lipid hydroperoxides, thiobarbituric acid reactive substances (TBARS)) and the activities of antioxidant system enzymes (superoxide dismutase, catalase, glutathione S-transferase) were analysed using standard methods. The results were processed using standard statistical methods. Results. Concentrations of heavy metals in P. polyantha gametophytes collected from the study area can be arranged in descending order as follows: Fe> Mn> Zn> Pb> Cu> Ni> Cr> Co> Cd. The content of individual metals in moss material varied depending on the sampling site. Moss shoots collected from zone B accumulated significantly higher levels of Cd, Co, Cu, Fe, Mn, Pb and Zn, and shoots from zone C had higher levels of Cr and Zn compared to those from the green zone. Moss gametophytes col­lected from sites in zone B had higher concentrations of LPO products and increased levels of antioxidant enzymes activity compared to the control. Conclusions. Elevated concentrations of heavy metals in moss material from sites exposed to traffic and industrial activities reflect atmospheric heavy metal pollution in urban areas. Intensive accumulation of heavy metals in gametophytes of P. polyantha that grows in the areas of transport activities is accompanied by increased lipid peroxi­dation processes and activation of enzymes of the antioxidant system in moss cells. Activation of antioxidant enzymes may play an important role in the adaptation of the moss P. polyantha to urban environments contaminated with heavy metals as a result of anthropogenic activities.

The The Analysis of Lead (Pb) Contents in Tuna (Thunnus sp.) Using Furnace Graph of Atomic Absorption Spectrophotometry Method

The The Analysis of Lead (Pb) Contents in Tuna (Thunnus sp.) Using Furnace Graph of Atomic Absorption Spectrophotometry Method

Heavy metals induced health risk assessment through consumption of selected commercially available spices in Noakhali district of Bangladesh

There are growing concerns for food safety due to the risks associated with heavy metal contamination of culinary herbs and spices in developing countries like Bangladesh. The objective of the present cross-sectional study is to determine the concentrations of the heavy metals Lead (Pb), Cadmium (Cd), Chromium (Cr), Copper (Cu), and Iron (Fe) in the branded and non-branded spices collected from the Noakhali district by Atomic absorption spectrophotometry method, as well as to assess the health hazard risk associated with heavy metals intake via consumption of spices. The findings revealed that the greatest concentrations of Pb (15.47 ± 1.93), Cd (1.65 ± 0.011), Cr (31.99 ± 3.97), Cu (18.84 ± 1.97), and Fe (9.29 ± 1.71) were found in Cardamom, Coriander leaf, Bay leaf, Dried chili, and Black pepper respectively. Around 37 % of Cr and 5 % of Fe Estimated Daily Intake (EDI) were greater than reference doses (RfD). All spices had Total Hazard Quotient (THQ) values for Pb, Cd, Cu, and Fe that were below acceptable, and 37 % of all spices had Total Target Hazard Quotient (TTHQ) values for Pb, Cd, Cu, and Fe that were over the standard range, suggesting adverse health impacts for consumers. Green chili, ginger, coriander leaf, and all kinds of chili powder and turmeric powder have been reported to have exceptionally high TTHQ levels of Cr. The estimated carcinogenic risk for chromium in non-branded coriander leaf was found to be higher than safe levels. This study provides valuable insights into the commonly consumed spices in Bangladesh and their potential health risks associated with heavy metal contamination. The findings of this study can be used by regulatory authorities to develop effective strategies and actions to mitigate these risks and safeguard public health.

Detection of heavy metals from Harpadon nehereus, Channa punctata and Pampus chinensis dried fishes and toxicity study of Channa punctata on embryonic zebrafish

ObjectivesGlobally, heavy metal pollution is one of the major serious environmental issues. The present investigations were carried out to determine the heavy metals concentrations from three dried fish along with the toxicity effects of Channa punctata dried fish on embryonic development of zebrafish. MethodsThe concentrations of heavy metals, Nickel (Ni), Chromium (Cr), Cadmium (Cd), Arsenic (As), and Lead (Pb) were detected from Harpadon nehereus, C. punctata and Pampus chinensis dried fish’s samples by Atomic Absorption Spectrophotometry (AAS) method. ResultsThe highest concentrations of As and Pb were 3.65 ± 0.08 and 3.29 ± 0.07 ppm, found in C. punctata followed by 3.11 ± 0.05 and 3.26 ± 0.07 ppm, in H. nehereus, respectively. Meanwhile, no significant levels of Ni, Cr and Cd were found in all the three tested dried fishes, compared with standard references. The treatments of 50 µg/ml and 100 µg/ml C. punctata dried fish solution on zebrafish embryos showed significant toxicity and abnormalities of unequal blastomeric curve, yolk sac edema, pericardial edema, spinal curvature and tail deformation at 24, 33, 48, 72 and 96 h post fertilization (hpf). ConclusionsThe present investigation revealed that the tested dried fishes contain heavy metals and C. punctata is highly toxic for zebrafish embryos.

Validation of the Randox colorimetric assays for serum copper and zinc.

Serum copper and zinc are measured to assess deficiency and toxicity. Atomic absorption spectrophotometry and mass spectrometry methods are expensive and require highly trained staff. Colorimetric assays are available from Randox which are inexpensive and can be automated. We validated serum copper and zinc colorimetric assays on the Binding Site Optilite analyser including comparison with flame atomic absorption spectrophotometry (FAAS) and inductively coupled plasma-mass spectrometry (ICP-MS). Accuracy, imprecision, lower limit of quantitation, and linearity were ascertained. The impact of triglycerides, bilirubin, nickel, and iron on assay performance was also investigated. Comparison of results from colorimetric analysis of patient and external quality assurance samples with those obtained by FAAS and ICP-MS was undertaken. Intra-, and inter-assay imprecision was <9%. Serum copper and zinc assays were linear between 1.8-35.6 and 2.3-45.7 µmol/L, respectively. Agreement was good between colorimetry and FAAS (intercept = -0.7, slope = 1.04) and ICP-MS (intercept = 0.6, slope = 0.99) for serum copper in patients' samples. For serum zinc, agreement was poor between colorimetry and FAAS (intercept = 2.2, slope = 0.87) and ICP-MS (intercept = 1.9, slope = 0.98) in patients' samples. There was a poor concordance in assessment of hypozincaemia between colorimetry and FAAS/ICP-MS. The Randox colorimetric assay for serum copper on the Optilite is simple to perform, has a short analysis time, and measured concentrations compare well with FAAS and ICP-MS. Due to poor agreement with FAAS and ICP-MS, colorimetry is not suitable for the measurement of serum zinc.

Pengaruh Cemaran Logam Berat Kadmium (CD) Terhadap Bentuk Sediaan Jahe Emprit (Zingiber Officinale Var. Amarum)

The high productivity of ginger in Indonesia ranks fifth out of the ten largest ginger producing countries in the world, for example emprit ginger (Zingiber officinale var. Amarum). Emprit ginger is usually processed into dosage forms such as powder and extract which are widely used as a spicy and off-flavor enhancer in drinks, food or traditional medicine. The growth of emprit ginger plants can be influenced by heavy metal contamination which has toxic effects and is easily absorbed, such as Cadmium (Cd), so that heavy metal Cd contamination needs to be considered as a quality requirement in the manufacture of traditional medicines and processed foods. This study aims to determine the levels and differences in heavy metal Cd contamination in the dosage form of emprit ginger using the Atomic Absorption Spectrophotometry (AAS) method. The type of research used is experimental research. The independent variable of this research is the dosage form of emprit ginger and the dependent variable is Cd metal content. The data obtained were then tested using the Independent Sample T-Test. The results of cadmium (Cd) metal contamination levels in the powder dosage form were an average of - 0.2183 mg/kg and thick extracts with an average of - 0.2225 mg/kg. The results of statistical tests using the Independent Sample T-Test showed that there was no influence of heavy metal Cd contamination on the dosage form of emprit ginger (p&gt;0.05).

Feasibility Study: Kapah Clam (Meretrix lyrata) Consumption Based on the Amount of Heavy Metal Lead (Pb) in Pulau Labu, Deli Serdang Regency

The hazardous heavy metal lead (Pb) can infiltrate the human body through the food chain. This study aimed to determine the maximum allowed amount of lead (Pb) and the lead heavy metal content in the meat of kapah clams (Meretrix lyrata) from Pantai Labu, Deli Serdang Regency, North Sumatra Province. This study used stratified random sampling in three locations: Bagan Beach, Muara Serdang, and Putra Deli. Heavy metal lead (Pb) measurements were conducted using the Parkin Elmer Atomic Absorption Spectrophotometry (AAS) method with an Atomic Absorption Spectrophotometer. Maximum Tolerable Intake (MTI) or Maximum Weekly Intake (MWI) is divided by the amount of heavy metal lead (Pb) in Kapah Clam meat (Meretrix lyrata) to determine the permissible consumption limit. As a result, exceeding the MTI limit can lead to toxicity.

Contamination Levels of Pb Heavy Metals and Availability of Phytoremediation Plants in the Gampong Jawa Landfill Area, Banda Aceh City

Heavy metal contamination is a major problem that occurs in landfill areas and contamination will continue to increase over time. This condition has a real impact on the increase in the accumulation of various kinds of materials which will have an impact on the occurrence of hazardous material accumulation such as the heavy metal lead (Pb). The purpose of this study was to determine the levels of heavy metal contamination of Pb found in the Gampong Jawa landfill area and the availability of phytoremediation plants as a mechanism to contain pollutant levels in the soil. The method of data collection was carried out by measuring the levels of lead accumulation (Pb) in groundwater in the TPA Gampong Jawa Kota Banda Aceh using the Atomic Absorption Spectrophotometry (AAS) method and for the availability of phytoremediation plants using an observational approach with a descriptive method. under the peacock (0.5648 ppm), under the neem (0.3031 ppm), under the orange garden (0.4438 ppm). The phytoremediation plants found included Samanea saman L, Leucaena leucocephala L, Cymbopogon citratus, Imperata cylindrica L, Desmodium ovalifolium, Azadirachta indica

Assessment of Phytoremediation Potential of Three Weed Plant Species in Soil Contaminated with Lead and Chromium

The study aimed to compare the tolerance capacity of Cyperus iria, Achyranthes aspera, and Eruca sativa to lead-contaminated and chromium-contaminated soils and to check their phytoremediation potential by pot culture experimentation. The experiment was conducted in three replicates in pots having 4 kg of sieved soil mixed with different doses of chromium, 0, 50, 100, 150, 200, 250, 300, and 350 mg·kg−1, and lead, 0, 100, 200, 300, 400, 500, 600, 700, and 800 mg·kg−1. The experiment was conducted for 80 days, and roots were harvested two times, i.e., at 40 days and 80 days after sowing. Metal accumulation in the roots was determined by the atomic absorption spectrophotometry method. The result of the study indicated that C. iria has the maximum potential to accumulate both the metals in its roots than other two plants. The order of chromium metal accumulation was found to be C. iria &gt; E. sativa &gt; A. aspera. On the other hand, the order of lead metal accumulation was found to be C. iria &gt; A. aspera &gt; E. sativa.

PROXIMATE ANALYSIS AND ESSENTIAL MINERALS CONTENT OF GASTROPODA Turbo setosus FROM LEMUKUTAN ISLAN, WEST KALIMANTAN

PROXIMATE ANALYSIS AND ESSENTIAL MINERALS CONTENT OF GASTROPODA Turbo setosus FROM LEMUKUTAN ISLAND, WEST BORNEO. Turbo setosus is a species of the gastropod class that has a wavy shell with circular lines on its surface. Because it is easy to find, this type of snail is widely used in the food sector. The nutritional value of Turbo setosus is also high so it has economic value. Proximate analysis and essential minerals are useful to determine the nutritional value contained. Gastropods were extracted using Soxhlet at a certain temperature to measure moisture content, fat content, ash content, and carbohydrates. To find out the number of carbohydrates, the by difference method is used. Meanwhile, to find out the amount of minerals contained using the Atomic Absorption Spectrophotometry (AAS) method.

Differences in Iron (Fe) Content in Samples of Refill Drinking Water (AMIU) and Bottled Drinking Water (AMDK) Atomic Absorption Spectrophotometry Method

Introduction: Iron is a metal in the first transition Series and the most common element on Earth by mass that makes up most of the outer and inner core of the Earth. High levels of iron (Fe) can be polluted by corrosive gases such as CO2 and H2S. In nature there are various elements that include heavy metals and also a component of pollutants, namely Cd, Hg, Cr, Pb, Ni, Cu, Zn, and Fe. The Aim Of The Research: To determine the difference in iron content in samples of drinking water refills and bottled drinking water Atomic Absorption spectrophotometric method. Research Method: This research was conducted at the mathematics and Natural Sciences Laboratory of Sriwijaya University in January-February 2022 with a total of 20 samples. The Result: The results of the study obtained the average value of iron (Fe) content in refilled drinking water samples 7.7 mg/L and bottled drinking water 3.7 mg/L Atomic Absorption Spectrophotometry method. Mann Witney test obtained the value of p (Sig 2 Tailed) = 0.04. Conclussions: Have different levels of iron in samples of drinking water refills and bottled drinking water Atomic Absorption spectrophotometric method.

Analysis of Calcium, Iron, Ash, Fat, and Water Content in Fresh and Processed Cow's Milk Using Atomic Absorption Spectrophotometry Methods

Milk is a source of nutrition for the body's growth, containing calcium and iron minerals. This study analyzes the optimal calcium, iron, ash, water, and fat levels in fresh and processed cow's milk. This study used five samples: pure milk (S1), pasteurized pure milk (S2), pasteurized milk with sugar (S3), pasteurized milk with paste coloring and citric acid (S4), and pasteurized milk with powder coloring(S5). The results showed that the calcium content of milk 4 (S4) treated with pasteurized milk added with sugar and paste coloring and citric acid had a higher calcium content than the other four samples by 98.61 mg/kg, at levels of milk iron 5 (S5) treated with pasteurized milk added with sugar and powdered dyes had a higher iron content than the other four samples by 73.81 mg/kg. The ash content of pure milk was higher at 0.96%. The fat content of pasteurized milk, the taste of coloring paste and powder, was higher than the other three samples at 2.80%. The water content of pasteurized added sugar milk was higher than the other four samples at 88.58%. Pure milk had the most optimal calcium (Ca) and iron (Fe) levels from the other four samples.

Iron Determination in Multivitamin Tablets: Enhancing Military Nutritional Preparedness Based Linear Regression Method

AAS is also an important method that has the most extensive application in metal analysis. most of the determinations were carried out by thin spectrophotometry, therefore an experiment was carried out to determine the Fe content in multivitamin tablets using the atomic absorption spectrophotometry method has high selectivity and sensitivity. Standard solution preparation. Prepare a standard solution of Fe with a concentration of 1, 2, 3, 4, and 5 ppm, then read the absorbance of the standard solution on AAS. Sample solution preparation and absorbance measurement. Several tablets were put into a beaker and 7 ml of concentrated HCl was added working principle of atomic absorption spectrophotometry is based on the evaporation of the sample solution which will initially be nebulized to form a spray, then it will be desolvated to form a dry aerosol until it is evaporated and converted into free atoms.. Iron levels in samples using AAS can be found by measuring the intensity of the radiation that decreases after being transmitted. The reduction in radiation intensity is proportional to the concentration of the sample element in the sample being measured. By testing the t-test, it was found that the AAS instrument was more sensitive to low concentrations

Nanocatalyst for carbon monoxide oxidation based on palladium(II), copper(II) salts, and carbon fiber material

The phase composition, textural, and activity of catalysts for the carbon monoxide low-temperature oxidation based on two series of non-woven carbon fiber material samples CFM-I and CFM-II, and K2PdCl4, Cu(NO3)2, KBr basic components were studied in this work. Catalysts and CFMs were studied by XRD, nitrogen and water vapor adsorption/desorption, and atomic absorption spectrophotometry methods. It has been found that the basic components K2PdCl4 and Cu(NO3)2 deposited on a microporous carbon surface undergo changes and form an X-ray amorphous Pd0 phase and a paratacamite—Cu2(OH)3Cl nanocrystalline phase. The catalysts were tested at carbon monoxide initial concentrations of 300 mg/m3 with an effective contact time of the catalyst with the gas–air mixture from 0.12 to 0.50 s. Catalysts provide air purification from CO to concentrations below MPCCO (20 mg/m3) and can be used in respiratory devices. The maximum catalytic activity is observed when the content of palladium in the catalyst is 1.70⋅10−4 mol/g and the content of copper (ІІ) is 4.68⋅10−4 mol/g.

Plasma zinc, copper and serum ceruloplasmin levels of autism spectrum disorder children in Bangladesh

Neural and cognitive processes require zinc and copper homeostasis and a normal zinc/copper ratio. Ceruloplasmin, an intrinsic antioxidant protein, maintains copper homeostasis, which might also influence autism spectrum disorder (ASD). ASD children are frequently reported with altered levels of these elements with wide geographical variations. This study evaluated any alteration in plasma zinc, copper, zinc/copper ratio and serum ceruloplasmin levels in Bangladeshi ASD children with respect to healthy controls. A cross-sectional study was conducted on 67 children aged 2 to 9 years of both sexes. Among them, 35 had ASD, while 32 were age, sex and body mass index (BMI) matched apparently healthy children. Plasma zinc and copper levels were estimated by the flame atomic absorption spectrophotometry method. Serum ceruloplasmin levels were estimated by the immunoturbidimetric method. Zinc and zinc/copper ratio in the 2–9 years old ASD children group were significantly lower (p=0.032 and p=0.002 respectively). On the other hand, copper (p=0.020) and ceruloplasmin (p = 0.045) levels were significantly higher than those of apparently healthy children. ASD was significantly associated with zinc deficiency (p=0.000) and copper toxicity (p=0.05). All children were again divided into 2–5 and 6–9 years age groups according to laboratory reference values for zinc and copper. Copper toxicity was significantly associated with ASD in the 2–5 years old age group (p=0.011), with a significant difference in plasma copper levels (p=0.009) and zinc/copper ratio (p=0.001) but not serum ceruloplasmin levels (p=0.110) compared to healthy controls. Serum ceruloplasmin was positively associated with plasma copper in ASD children of all age groups. This study shows that ASD in Bangladesh can be associated with low plasma zinc and high plasma copper and serum ceruloplasmin levels.

IDENTIFICATION AND CHARACTERIZATION OF CASSAVA MUTANT GENOTYPES WITH HIGH LEAF MINERAL CONTENT AT THE MV10 GENERATION

Cassava leaves’ consumption as vegetables are common in several Asian countries. They contain various nutrients, such as, anthocyanins, carotene, minerals, and vitamins. New varieties with high mineral contents in leaves need development to increase the leaves’ quality as vegetables. This study aimed to identify and characterize cassava genotypes of the MV10 generation resulting from gammaray- induced mutations based on the leaves' morphology, growth characteristics, and mineral contents, using two cultivars and 10 cassava genotypes. All genotypes planted in a randomized complete block design had three replications. The leaf's morphology and mineral contents underwent scrutiny on the third to fifth leaf below the apical shoot. The mineral content analysis used the Atomic Absorption Spectrophotometry method. Data analysis comprised the ANOVA test and descriptive analysis. This study revealed petiole length, leaf lobe length, and leaf lobe width were significantly different between genotypes at four months after planting (MAP). However, no meaningful difference showed for the plant height, stem diameter, and number of leaves among genotypes. In general, the leaf morphology consisted of purplish green apical leaves with present pubescence; the shape of the central leaflet is lanceolate; the petiole color is reddish to greenish; leaves are dark green with five to seven leaf lobes; leaf veins are reddish-green; smooth lobe margins; and horizontal petiole orientation. The genotypes affect mineral contents, as the genotype with the highest Mg contents was G6-2-15-5-3, and the one with the highest Zn contents was G2D1-422. Fe contents showed more variations between genotypes, and no genotype showed consistently high Fe contents. This research produced promising genotypes for Mg or Zn contents in leaves for future cassava varieties for vegetable production.