Ammonia emissions from agriculture are an environmental and human health concern, and there is increasing pressure to reduce emissions. Animal agriculture is the largest global source of ammonia emissions and on a per cow basis dairy operations are the largest emitters. The storage and disposal of the dairy waste is one area where emissions can be reduced, aerobic biological treatment of wastewater being a common and effective way of reducing ammonia emissions. An aeration experiment in a dairy lagoon with two commercial aerators was performed for 1 month. Liquid concentrations of ammonia, total nitrogen, nitrite and nitrate were monitored before, during and after the experiment and atmospheric ammonia was measured downwind of the lagoon using a short-path differential optical absorption spectroscopy (DOAS) instrument with 1ppbv sensitivity. No changes in either liquid or atmospheric ammonia concentrations were detected throughout the experiment, and neither dissolved oxygen, nitrite nor nitrate could be detected in the lagoon at any time. The average ammonia concentration at 10 sampling sites in the lagoon at a depth of 0.15m was 650mgl−1 and at 0.90m it was 700mgl−1 NH3–N. The average atmospheric ammonia concentration 50m downwind was about 300ppbv. The 0.90m depth total nitrogen concentrations and total and volatile solids concentrations decreased during the experiment due to some mixing of the lagoon but the 0.15m depth concentrations did not decrease indicating that the aerators were not strong enough to mix the sludge off the bottom into the whole water column.