We present the atmospheric characterization of the substellar companion HD 19467 B as part of the pioneering James Webb Space Telescope Guaranteed Time Observer program to obtain moderate resolution spectra (R ∼ 2700, 3–5 μm) of a high-contrast companion with the NIRSpec integral field unit (IFU). HD 19467 B is an old, ∼9 Gyr, companion to a solar-type star with multiple measured dynamical masses. The spectra show detections of CO, CO2, CH4, and H2O. We forward model the spectra using Markov Chain Monte Carlo methods and atmospheric model grids to constrain the effective temperature and surface gravity. We then use NEWERA-PHOENIX grids to constrain nonequilibrium chemistry parameterized by K zz and explore molecular abundance ratios of the detected molecules. We find an effective temperature of 1103 K, with a probable range from 1000 to 1200 K, a surface gravity of 4.50 dex, with a range of 4.14–5.00, and deep vertical mixing, log10(K zz ), of 5.03, with a range of 5.00–5.44. All molecular mixing ratios are approximately solar, leading to a C/O ∼ 0.55, which is expected from a T5.5 brown dwarf. Finally, we calculate an updated dynamical mass of HD 19467 B using newly derived NIRCam astrometry, which we find to be 71.6−4.6+5.3MJup , in agreement with the mass range we derive from evolutionary models, which we find to be 63–75 M Jup. These observations demonstrate the excellent capabilities of the NIRSpec IFU to achieve detailed spectral characterization of substellar companions at high contrast close to bright host stars, in this case at a separation of ∼1.″6 with a contrast of 10−4 in the 3–5 μm range.