The cones of coniferous species are a waste biomass byproduct that can be potentially utilized for a variety of purposes. One of the many application fields is the extraction of bioactive materials, particularly antioxidant polyphenols. Scientific literature on the antioxidant content of coniferous cones at different ripening stages is limited. In this study, we conducted a comparative analysis of the antioxidant content of selected taxa that are either common in Hungary or that have not yet been investigated in the scientific literature in any great detail (Cedrus atlantica, Larix decidua, Picea abies, Pinus mugo, Pinus nigra, Pinus sylvestris, Pinus wallichiana, Tsuga canadensis, Tsuga heterophylla, Chamaecyparis lawsoniana, Taxodium distichum, Thuja occidentalis, Metasequoia glyptostroboides, Thuja orientalis, Cryptomeria japonica, Cunninghamia lanceolata). A comparison of green, mature and opened cones was performed for the assigned taxa. Folin-Ciocâlteu total polyphenol content (TPC), ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays were used to assess the antioxidant contents. Overall antioxidant power was determined by a scoring system that combined the three assay results. In general, best values were found for green cones, followed by mature, and opened cones for each taxon. Tsuga canadensis, Metasequoia glyptostroboides, Chamaecyparis lawsoniana, Cryptomeria japonica, Thuja orientalis and Picea abies all contained high amounts of antioxidants in both green and mature cones and attained the highest scores. High-performance liquid chromatographic/tandem mass spectrometric profiling of the cone polyphenols was also completed for selected samples. Results provide a basis for future bioactivity testing of these samples.