South American Atlantic Research Articles

Inferring Evolution of Habitat Usage and Body Size in Endangered, Seasonal Cynopoeciline Killifishes from the South American Atlantic Forest through an Integrative Approach (Cyprinodontiformes: Rivulidae).

Cynopoecilines comprise a diversified clade of small killifishes occurring in the Atlantic Forest, one of the most endangered biodiversity hotspots in the world. They are found in temporary pools of savannah-like and dense forest habitats, and most of them are highly threatened with extinction if not already extinct. The greatest gap in our knowledge of cynopoecilines stems from the absence of an integrative approach incorporating molecular phylogenetic data of species still found in their habitats with phylogenetic data taken from the rare and possibly extinct species without accessible molecular information. An integrative analysis combining 115 morphological characters with a multigene dataset of 2,108 bp comprising three nuclear loci (GLYT1, ENC1, Rho), provided a robust phylogeny of cynopoeciline killifishes, which was herein used to attain an accurate phylogenetic placement of nearly extinct species. The analysis indicates that the most recent common ancestor of the Cynopoecilini lived in open vegetation habitats of the Atlantic Forest of eastern Brazil and was a miniature species, reaching between 25 and 28 mm of standard length. The rare cases of cynopoecilines specialized in inhabiting pools within dense forests are interpreted as derived from four independent evolutionary events. Shifts in habitat usage and biogeographic patterns are tentatively associated to Cenozoic paleogeographic events, but the evolutionary history of cynopoecilines may be partially lost by a combination of poor past sampling and recent habitat decline. A sharp evolutionary shift directed to increased body size in a clade encompassing the genera Campellolebias and Cynopoecilus may be related to a parallel acquisition of an internally-fertilizing reproductive strategy, unique among aplocheiloid killifishes. This study reinforces the importance of adding morphological information to molecular databases as a tool to understand the biological complexity of organisms under intense pressure from loss of habitat.

GPS and in situ Swarm observations of the equatorial plasma density irregularities in the topside ionosphere

Here we study the global distribution of the plasma density irregularities in the topside ionosphere by using the concurrent GPS and Langmuir probe measurements onboard the Swarm satellites. We analyze 18 months (from August 2014 till January 2016) of data from Swarm A and B satellites that flew at 460 and 510 km altitude, respectively. To identify the occurrence of the ionospheric irregularities, we have analyzed behavior of two indices ROTI and RODI based on the change rate of total electron content and electron density, respectively. The obtained results demonstrate a high degree of similarities in the occurrence pattern of the seasonal and longitudinal distribution of the topside ionospheric irregularities derived from both types of the satellite observations. Among the seasons with good data coverage, the maximal occurrence rates for the post-sunset equatorial irregularities reached 35–50 % for the September 2014 and March 2015 equinoxes and only 10–15 % for the June 2015 solstice. For the equinox seasons the intense plasma density irregularities were more frequently observed in the Atlantic sector, for the December solstice in the South American–Atlantic sector. The highest occurrence rates for the post-midnight irregularities were observed in African longitudinal sector during the September 2014 equinox and June 2015 solstice. The observed differences in SWA and SWB results could be explained by the longitude/LT separation between satellites, as SWB crossed the same post-sunset sector increasingly later than the SWA did.

Pan-American marine coastal distribution of the acanthocephalanProfilicollis altmanibased on morphometric and phylogenetic analyses of cystacanths from the mole crabEmerita brasiliensis

Thorny-headed acanthocephalan worms of the genus Profilicollis are widely distributed in the oceans of the world and present complex life cycles with intermediate and definitive hosts. The genus is still poorly known, with an unstable taxonomy and, for most species, incompletely characterized geographical distributions. In this study, based on molecular and morphological evidence, we report that the species Profilicollis altmani is also distributed along the South American Atlantic coast, using the mole crab Emerita brasiliensis as an intermediate host. As such, our record shows that P. altmani has a Pan-American distribution where five species of Emerita are utilized as intermediate hosts.

Water quality in Atlantic rainforest mountain rivers (South America): quality indices assessment, nutrients distribution, and consumption effect.

The South American Atlantic rainforest is a one-of-a-kind ecosystem considered as a biodiversity hotspot; however, in the last decades, it was intensively reduced to 7% of its original surface. Water resources and water quality are one of the main goods and services this system provides to people. For monitoring and management recommendations, the present study is focused on (1) determining the nutrient content (nitrate, nitrite, ammonium, and phosphate) and physiochemical parameters (temperature, pH, electrical conductivity, turbidity, dissolved oxygen, and total dissolved solids) in surface water from 24 rainforest mountain rivers in Argentina, (2) analyzing the human health risk, (3) assessing the environmental distribution of the determined pollutants, and (4) analyzing water quality indices (WQIobj and WQImin). In addition, for total coliform bacteria, a dataset was used from literature. Turbidity, total dissolved solids, and nitrite (NO2 (-)) exceeded the guideline value recommended by national or international guidelines in several sampling stations. The spatial distribution pattern was analyzed by Principal Component Analysis and Factor Analysis (PCA/FA) showing well-defined groups of rivers. Both WQI showed good adjustment (R (2) = 0.89) and rated water quality as good or excellent in all sampling sites (WQI > 71). Therefore, this study suggests the use of the WQImin for monitoring water quality in the region and also the water treatment of coliform, total dissolved solids, and turbidity.

Morpho-histodifferentiation of Billbergia Thunb. (Bromeliaceae) nodular cultures.

Nodule cultures are formed through an intermediate morphogenetic route that lies between organogenesis and somatic embryogenesis. Although well described in many species, different aspects of the morphological and histological development of nodules remain to be clarified. Based on their threatened status and high ornamental value, Billbergia alfonsi-joannis and Billbergia zebrina, two epiphytic bromeliad species endemic to the South American Atlantic Forest, were studied. Nodular cultures were induced to grow from nodal segments taken from etiolated seedlings grown in vitro for 12weeks in the dark on MS medium supplemented with 1μM TDZ. Samples were taken for analysis weekly over 8weeks of growth and analyzed under light, transmission electron, and scanning electron microscopes. Morphological and histological analysis showed that nodular clusters originated from stem pericycles and consisted of a polycenter, cambial tissue, cortical parenchyma, and a covering tissue. The polycenter consisted of an organizational center dispersed in parenchymal tissue. Each organizational center was formed by a vascular system surrounded by a bundle sheath. A cambial tissue surrounded these polycenters, promoting the regeneration of new nodules and leading to the formation of buds and roots. Primary nodules could generate secondary nodules in a repetitive process. Thus, histological analysis revealed the origin and formation of nodular cultures. These new data will support the establishment of micropropagation protocols and regeneration on a large scale for these species.

Identification of the Toxic Pentapeptide Nodularin in a Cyanobacterial Bloom in a Shrimp Farm in South American Atlantic Coast

Since 2010, blooms of the brackish cyanobacteria Nodularia spumigena are recurrent in the shrimp growth tanks of the Marine Aquaculture Station during summer in Southern Brazil. Cyanobacterial growth led to a decrease in the white shrimp Litopenaeus vannamei productivity. In the summer of 2014, a Nodularia bloom was collected from the tanks; filaments were separated by flotation and washed thoroughly twice in F/2 culture medium. Healthy filaments were lyophilized and the powder used for nodularin quantification by HPLC-DAD and immunoassays. Nodularin containing lyophilized powder was also tested for toxicity against the brine shrimp Artemia salina post-larvae and the white shrimp Litopenaeus vannamei 35 days old larvae. The lyophilized Nodularia powder contained 1.88 mg of the toxin nodularin g-1d.w. Its toxicity was confirmed in bioassays with Artemia salina and Litopenaeus vannamei giving an LC50 of 1.22 and 2.50 μgL-1 of nodularin, respectively. This paper firstly describes the occurrence and the toxicity of nodularin in South Atlantic coastal waters with consequences to shrimp farming.

Speculations on the impact of catastrophic subduction initiation on the Earth System

The physics of subduction initiation can be studied with numerical models of lithosphere dynamics, to the extent where we can now test the potential consequences of a catastrophic subduction initiation event on the Earth System. The South American Atlantic passive margin is here used to show that, once subduction has catastrophically initiated there, a major geodynamic reconfiguration of the South American plate (SAm) is likely to take place: (1) compression in the east will be inverted to extension, because ridge push will be replaced by subduction rollback and trench retreat; (2) compression in the west will be inverted to extension due to absolute rollback; and (3) without buttressing from the east and west, the Andes will collapse. Extension at both margins of continental SAm will produce two new volcanic arcs, several thousands of kilometres long each, bounded by trenches and two new back-arc basins. The spreading rate of the Mid-Atlantic Rift will significantly increase, because of the cumulative effect of ridge-push and slab-pull in the same direction. The substantially increased volcanism all around SAm and in the MAR will most likely release massive amounts of greenhouse and (toxic) gases into the atmosphere and oceans, which might lead to major oceanic and atmospheric circulation changes. We present new numerical modelling that supports the proposed evolution of the SAm after subduction has catastrophically initiated at its eastern passive margin.

Genetic diversity of freshwater fishes from the South American Atlantic Rainforest: The case study of the genus Phalloceros

The Atlantic Rainforest is one of the global conservation hotspots, representing a highly threatened area with elevated levels of endemism and species richness. Fish species from this area are one of the most exploited vertebrate groups by the international aquarium trade, an industry with an annual trade volume of about U$15–25 billions. The native South American genus Phalloceros includes species commonly known as “guppies”, being found not only in South America, but also New Zeeland, Australia and Malawi, where they have been secondarily introduced with several ecological effects. We used SNPs and indels in mitochondrial DNA (mtDNA) to investigate the molecular diversity of the species Phalloceros anisophallos from coastal Atlantic drainage areas in Rio de Janeiro state, Brazil. Our aim is to develop a panel of mitochondrial markers to be used for the identification of guppy fish species. The molecular analyses were carried out using novel (n=28) sequences from the mitochondrial COI gene (for DNA Barcoding) and the 16S rDNA gene (for SPInDel). We were able to determine the molecular variability of the distinct populations of P. anisophallos, which showed distinct patterns of shared polymorphism.

Effects of neutrality, geometric constraints, climate, and habitat quality on species richness and composition of Atlantic Forest small-mammals

Aim To compare the fit of models of climate, habitat quality, neutral processes, and geometric constraints to species richness and composition of small mammal assemblages. Location The South American Atlantic Forest biome. Methods Using neutral models and mid-domain effect models, we simulated species spread in a spatially explicit array of grid cells representing the Atlantic Forest domain. We compared empirical patterns of species richness and composition with predictions of the neutral and mid-domain effect models. We also modeled individual species responses to climatic conditions and forest integrity, a measure of habitat quality. Results Habitat quality was the single best predictor of local species richness (α-diversity), but was a poor predictor of local species composition and of the decay in species similarity with distance (β-diversity). The neutral and mid-domain models generated very similar predictions, and were better predictors of species composition than of species richness. Climate variables were also strongly associated with overall species composition, but not with species richness. Main Conclusions The species richness of small-mammal assemblages in the Atlantic Forest is best explained by variation in habitat quality. In contrast, the composition of small-mammal assemblages is best explained by models of limited dispersal (neutral and mid-domain) and effects of climate on local species composition. Collectively, these results suggest that regional patterns of species richness may be uncoupled from patterns of species composition. Both species richness and composition should be considered when evaluating the predictions of neutral and mid-domain effect models, and of correlations of community structure with climatic or habitat variables.

Parasites of Urophycis brasiliensis (Gadiformes: Phycidae) as indicators of marine ecoregions in coastal areas of the South American Atlantic.

The potential value of parasites as ecosystem markers was tested by analyzing the metazoan assemblages of Urophycis brasiliensis caught in four locations distributed in three ecoregions of the Warm Temperate Southwestern Atlantic. A total of 5,001 metazoan parasites belonging to 33 species were found. The identified parasites varied across locations in terms of presence, prevalence, and abundance, and their multivariate analyses resulted in clear similarity patterns. No differences were observed between two locations of the same ecoregion, whereas an evident separation of samples was observed across ecoregions in support of the existing hypotheses regarding the ecoregional division of the southwestern Atlantic. We proposed that parasite assemblages, which are composed of several metazoan phyla, are potentially useful as ecosystem indicators. This suggestion is derived from the combined evidence of the evolutionary history and biogeography of multiple lineages, which is expected to be more efficient in capturing recurrent patterns in overall biodiversity than individual lineages. Furthermore, as many parasites have complex life cycles, their distribution patterns are dependent not only on environmental conditions but also on the distribution and population density of all hosts involved in their life cycles, adding further sources of distributional variability that act synergistically to define robust geographical patterns. The selection of long-lived parasites and their comparative analysis provided evidence supporting the existence of three different stocks in the four sampled areas. The best parasite tags were those with low specificity in fish hosts, constituting promising biological tags for the stock discrimination of other fish species in the region.

First occurrence of Beroe forskalii (Ctenophora) in South American Atlantic coastal waters, with notes on the use of macrociliary patterns for beroid identification.

Beroe forskalii Milne Edwards, 1841 is an oceanic ctenophore with a global distribution. The present study provides the first record of Beroe forskalii for the South American Atlantic coast, including a redescription of the species and a discussion on the utility of macrociliary patterns for the correct identification of at least some beroid species, exemplified by a comparison of the macrociliary patterns of Beroe forskalii and Beroe ovata (Chamisso & Eysenhardt, 1821).

Population Dynamics and Diet of the Madamango Sea Catfish Cathorops spixii (Agassiz, 1829) (Siluriformes: Ariidae) in a Tropical Bight in Southeastern Brazil

The madamango sea catfish, Cathorops spixii (Siluriformes: Ariidae), is often among the most abundant fishes on the South American Atlantic coast. In the present study, conducted in shallow, non-estuarine coastal areas of Caraguatatuba Bight in southeastern Brazil, collections of this species, the most abundant member of the ichthyofauna, included primarily medium-sized individuals, indicating that the area may play a specific role in their development. Although studies of the local ichthyofauna have been much neglected, the area is economically important and its ecological significance is undervalued. This study primarily treats habitat use by C. spixii, assessing certain population parameters and the dietary composition. Monthly samples were taken from August 2003 through October 2004, with three trawls in two areas, corresponding to depths of about 1 to 4 m. The catfish showed two main peaks of abundance in the area, in April/May and July 2004. A mode around 9 cm SL persisted through time, and the entrance of younger recruits peaked from January to April. The low estimate for body-growth parameters (K = 0.16) corroborates some K-strategist characteristics of the species. The asymptotic length was 27.3 cm SL and total mortality (Z) was 1.01 yr−1. Cathorops spixii showed an omnivorous feeding habit, preying mainly upon polychaetes, copepods and bivalves, with considerable seasonality in its diet.

Feeding ecology of Kelp Gulls (Larus dominicanus) in marine and limnetic environments

The diet of the Kelp Gull (Larus dominicanus) was investigated at the northern limit of its distribution along the South American Atlantic coast. We used two complementary methods, pellet analysis and stable isotope analysis (SIA), to describe and compare Kelp Gull feeding ecology in freshwater and marine environments. The assimilated diet over two different time scales was investigated via SIA of plasma and red blood cells, blood components with different turnover rates. Fish composed the bulk of the diet of Kelp Gulls in both marine (White Croaker Micropogonias furnieri and Banded Croaker Paralonchurus brasiliensis) and limnetic areas (Armoured Catfish Loricariidae and La Plata Croaker Pachyurus bonariensis), despite the importance of benthic prey from the intertidal zone in samples collected from the marine environment (Wedge Clam Donax hanleyanus and the Yellow Clam Mesodesma mactroides). The fish consumed by the gulls were common discards from fisheries in both environments, and marine bivalves were found at a high density at the marine beach. Diet varied between the different time scales analysed. Conventional diet data generally agreed with stable isotope model estimates, emphasising the importance of using complementary approaches in dietary studies.

Diversification of Morpho butterflies (Lepidoptera, Nymphalidae): a re‐evaluation of morphological characters and new insight from DNA sequence data

This study compiles previously published morphological, colour and behavioural characters and includes new DNA sequence data for eight markers (one mitochondrial and seven nuclear) to re‐evaluate phylogenetic relationships and estimate times of divergence for Morpho butterflies using parsimony and Bayesian methods. We note an effect of missing data on phylogenetic inference and calculations of Partitioned Bremer Support. Morphology and DNA trees were moderately congruent, and the combined analyses of all data included elements of both sources. Both morphology and DNA support the monophyly of Morpho and the early separation of the sister pair M. marcus plus M. eugenia, but trees from different data sources are congruent mostly at derived nodes, and differ at several internal nodes. The analyses of combined data indicate that Morpho is composed of four clades each of which include one or more previously proposed subgenera. The subgenera Pessonia and Morpho were not monophyletic, and to address this issue we propose that Pessonia, syn.nov. be subsumed within Morpho. The ancestor of Morpho probably arose during the Oligocene, and most diversification seems to have occurred during the late Miocene. S‐DIVA analysis suggests eastern Andean region as the ancestral area for Morpho, and that the South American Atlantic Forest was colonized multiple times.

Early morning enhancement in ionospheric electron density during intense magnetic storms

This study reports on ionospheric disturbances that occurred in the early morning hours in the South American–Atlantic sector during a few intense/super storm events. The events were observed at latitudes close to the southern crest of the equatorial ionization anomaly (EIA) as an unusual intensification of the F region electron density peak at local times when the EIA is not usually developed. All the events were observed at pre dawn-morning hours, under conditions of northward interplanetary geomagnetic field. Large scale traveling ionospheric disturbances that are launched during highly disturbed conditions and/or equatorward surges in the thermospheric meridional winds seem to be the most probable causes of the observed disturbances.

The bertillonage in the South American Atlantic World

The bertillonage in the South American Atlantic World

Malacofauna Cuaternaria del litoral norte de Santa Cruz (Patagonia, Argentina)

The molluscan content from three “marine terraces” (TM) preserved along 250 km of the Santa Cruz province provided 49 molluscan taxa (33 Gastropoda and 16 Bivalvia) and the first Quaternary records for Ataxocerithium pullum, Crepidula protea, C. aculeata, Adelomelon ferrusacii, Brachidontes rodriguezi, Aequipecten tehuelchus, Ostrea tehuelcha, and Solen tehuelchus. The mollusca assemblages suggest typical marine nearshore conditions similar to the adjacent modern littoral (Southwestern Atlantic), with mostly hard substrates, shallow and cold waters. The TMVI (+ 5–15 m a.s.l., ca. 8–1.2 ka A.P.) is mainly characterized by dominance of Brachidontes spp., Nacella spp. and Mytilus edulis, associated with Siphonaria lessoni. No evidence has been found for the TMVI of clear biotic responses or strong palaeobiogeographic effects during the time span of the Climatic Optimum (“Hypsithermal”, mid-Holocene) which has been documented for other coastal areas northward in Argentina, down to ca. 44–45° S. Scarce differences distinguish the TMV (+ 15–30 m a.s.l., ca. 108–137 ka A.P., MIS5?) and TMIV (+ 20–30 m a.s.l., ca. 242 ka A.P.) regarding the Holocene and the modern littoral. Within the TMV, Clausinella, Eurhomalea, Solen tehuelchus and Brachidontes cf. darwinianus (typically in warmer waters) are exclusive; in the TMIV, Trophon, Buccinanops, Brachidontes and Pectinidae are characteristic. Predominance of Trophon spp. (typically in cold Magallanic waters) in modern samples vs. scarcity or absence in the TM suggest slightly lower modern SST. Several taxonomic similitudes with the Pacific Sudamerican coast (e.g., Fissurella, Nacella, Tegula, Crepidula, Trochita, and Acanthina) could respond to cold currents helping cold-water immigrants to join southern Southamerican Atlantic after the opening of the Drake Passage.

A new species of Dichelyne (Nematoda, Cucullanidae) parasitizing sciaenid fishes from off the South American Atlantic coast

AbstractA new nematode species Dichelyne (Cucullanellus) sciaenidicola sp. nov. is described based on specimens collected from the Whitemouth croaker Micropogonias furnieri (Desmarest) and the Argentine croaker Umbrina canosai Berg, from coastal waters of Argentina and Brazil. These nematodes were firstly identified as D. (C.) elongatus (Törnquist, 1931), a commonly reported species from M. furnieri in South American Atlantic waters. However, other species of Dichelyne have so far been reported from this host in the same area, namely D. (C.) rodriguesi (Pinto, Fábio et Noronha, 1970), D. (C.) amaruincai (Freitas, Vicente et Ibañez, 1969) and D. (Dichelyne) micropogonii Pereira et Costa, 1996. A careful re-examination of these parasites, as well as of type specimens of all species reported from M. furnieri, revealed that these nematodes represented a new species. The new species is distinguished from most of its congeners by having papillae 5–7 and 9 forming a subventral line close to cloaca, this feature is shared with other 6 species [D. (C.) dichelyneformis (Szidat, 1950), D. (C.) fraseri (Baylis, 1929), D. (C.) abbreviatus (Rudolphi, 1819), D. (C.) adriaticus (Törnquist, 1931), D. (C.) minutus (Rudolphi, 1819) and D. (C.) mariajuliae Alarcos, Timi, Etchegoin et Sardella, 2006)], which are readily distinguished by their body size, spicules length, distribution patterns of other papillae and position of the excretory pore and deirids. Also, D. (C.) elongatus from Umbrina canariensis (Valenciennes) from West Africa is established as a new species Dichelyne (Cucullanellus) yvonnecampanae sp. nov.; D. (C.) amaruincai from Pacific waters is considered as a valid species, D. (D.) micropogonii is regarded as species inquirendae and D. (C.) rodriguesi is identified as Cucullanus sp.

On the protection of "protected areas".

Tropical moist forests contain the majority of terrestrial species. Human actions destroy between 1 and 2 million km(2) of such forests per decade, with concomitant carbon release into the atmosphere. Within these forests, protected areas are the principle defense against forest loss and species extinctions. Four regions-the Amazon, Congo, South American Atlantic Coast, and West Africa-once constituted about half the world's tropical moist forest. We measure forest cover at progressively larger distances inside and outside of protected areas within these four regions, using datasets on protected areas and land-cover. We find important geographical differences. In the Amazon and Congo, protected areas are generally large and retain high levels of forest cover, as do their surroundings. These areas are protected de facto by being inaccessible and will likely remain protected if they continue to be so. Deciding whether they are also protected de jure-that is, whether effective laws also protect them-is statistically difficult, for there are few controls. In contrast, protected areas in the Atlantic Coast forest and West Africa show sharp boundaries in forest cover at their edges. This effective protection of forest cover is partially offset by their very small size: little area is deep inside protected area boundaries. Lands outside protected areas in the Atlantic Coast forest are unusually fragmented. Finally, we ask whether global databases on protected areas are biased toward highly protected areas and ignore "paper parks." Analysis of a Brazilian database does not support this presumption.

Community ecology of the metazoan parasites of namorado sandperches, Pseudopercis numida Miranda-Ribeiro, 1903 and P. semifasciata Cuvier, 1829 (Perciformes: Pinguipedidae), from the coastal zone of the State of Rio de Janeiro, Brazil

One hundred and twenty-eight specimens of namorado sandperches, 62 P. numida and 66 P. semifasciata, collected between October 2002 and June 2003 off the Cabo Frio, Rio de Janeiro, Brazil (Lat 23 degrees S and Long 42 degrees W), were examined to study their metazoan parasites. Parasite communities of these fish were composed basically of endoparasites, mainly digenean and cestodes species, with low prevalence and abundance but having high parasite species richness values (at the component community level). Among these values, that found for P. numida is the highest so far recorded for marine fishes from the Neotropical Region. Thirty-nine species of metazoan parasites were collected: 36 from P. numida and 28 from P. semifasciata. Twenty-five parasite species were common to both species of namorado sandperches. Pseudopercis numida and P. semifasciata are new host records for all parasite species collected, with the exception of Microcotyle pseudopercis. Choanodera sp., Leurodera decora, Neolebouria georgenascimentoi, and Proctoeces sp. which were recorded for the first time in the South American Atlantic Ocean. Gnathia sp. from P. numida and Scolex pleuronectis from P. semifasciata were the species having the greatest dominance frequency. Parasite abundance in P. numida and P. semifasciata were positively correlated with the host total length. Only in P. numida was parasite species richness correlated positively with the host total length. No significant differences between endoparasite infracommunities of P. numida and P. semifasciata were detected. The ectoparasites of P. numida had higher values for parasite abundance, parasite richness, Brillouin index, evenness index, and Berger-Parker index than those of the ectoparasites of P. semifasciata. Comparisons among all ecto- and endoparasites showed the ectoparasites of P. numida as the most heterogeneous group. Low similarity values were observed among the three types of parasite infracommunities of the two hosts.