Atlantic Forest Research Articles

Facilitation and allelopathy mediate phylogenetic and functional diversity under Atlantic Rainforest trees

Plant phylogeny, diversity, and production of germination inhibiting chemicals can be used as patterns for inferring key drivers of plant community construction and assembly. We conducted the study in a restoration area of Atlantic Rainforest from Southeast Brazil. In this context, we aimed to investigate community assembly rules by simultaneously evaluating the relationships of species with a phylogenetic, functional, and ecophysiological (allelopathy) approach and multifaceted β diversity (taxonomic, phylogenetic, and functional). We measured the plant canopy height and diameter at soil height for all individuals and determined successional group and dispersal syndrome for all species regenerating in open areas and below 18 adult individuals of each tree species Bixa atlantica Antar & Sano, Inga laurina (Sw.) Willd., Joannesia princeps Vell. and Senna multijuga subsp. multijuga var. verrucosa (Vogel) H.S.Irwin & Barneby. Phylogenetic and functional indexes of community structure were calculated with the net relatedness index and the nearest taxon index. Taxonomic, phylogenetic, and functional diversities (respectively, TD, PD, and FD) for the regenerating community in each area were calculated and pairwise comparisons were made for TD, FD, and PD components of β diversity. Tests of tree species allelopathy derived from leaf extract were performed with seeds of Lactuca sativa L. (Asteraceae). The effects of diversity and extracts on seed germination were analyzed using generalized linear models. The phylogenetic and functional structure of the regenerating communities under the four tree species did not differ from random for the four studied tree species. The PD was significantly higher for the communities that regenerated under Joannesia, while the FD was higher under both Joannesia and Senna, compared to communities from open sites, evidencing a facilitation mechanism for these two species. Only Senna and Inga differed from random in relation to β TD and β FD, suggesting possible environmental changes in these areas. Seeds irrigated with the extracts of Inga, Joannesia, and Senna inhibited germination in an intermediate way, whereas seeds irrigated with Bixa extract had only 1 % germination, indicating allelopathic effects. In this way, the planted species directly influence the organization of communities that regenerate below their canopies. It is therefore important to choose species that can contribute to improving diversity, and thus favor the success of restoration projects.

Minor loss or huge threat? Assessment of potential infrastructure projects reveals drastic landscape degradation in the Atlantic forest

Projects demanding deforestation to expand urban infrastructure and road networks put pressure on the Atlantic Forest − a hyper-fragmented global conservation hotspot known for its high biodiversity and endemism. To support decision-making, we applied a scientific approach to analyse the immediate and long-term impacts of two major planned projects in the most threatened Atlantic Forest region – the Pernambuco Endemism Center, a crucial area for the survival of many endemic species. A Metropolitan Road Arch and a Training School for Career Sergeants are expected in the Environmental Protection Area Aldeia-Beberibe (31,634 ha) in the next few years. The area has 198 protected forest remnants, including one larger than 10,000 ha. Our analysis shows that the projects’ implementation may deforest 189 ha, increase fragment number by 3.5 % and decrease the average patch area by 4 %. The largest fragment will be divided into two. We may lose 335,307 trees and 44,655.59 Mg of carbon. Approximately 112.67 ha of watershed protection will be damaged. Thus, in the medium to long term, habitat quality will worsen, and the probability of connectivity will decrease, restricting forest-dependent species’ mobility considerably. To date, alternative areas have been proposed in meetings, working groups, and environmental advisory bodies for the analysed projects.We strongly oppose further deforestation and suggest that science-based approaches must be immediately considered for any project that requires forest suppression in highly fragmented landscapes. This is crucial to mitigate the alarming scenarios of extreme climate and biodiversity loss that we are currently witnessing in Brazil and worldwide.

Numerical assessment of changes in land–atmosphere interactions during the rainy season in South America using an updated vegetation map

AbstractLand Use and Land Cover Change (LULCC) is a key driver of changes in land–atmosphere interactions, playing an essential role in climate and weather patterns in South America (SA). This study used a modelling approach to assess the land–atmosphere changes introduced by LULCC over the Brazilian territory. Numerical experiments of early (2006), neutral (2004) and late (2008) rainy season onset years were performed in the Integrated BIosphere Simulator (IBIS) to assess the land‐surface parameters, and in Brazilian Atmospheric Model (BAM) to assess the atmospheric variables. The models were run with a natural (NAT) vegetation map and an updated (UP) vegetation map, which incorporated realistic Brazil deforestation up to the early 2000s. The differences between the two maps were particularly larger over 25°–15°S and 50°–40°W, where the Atlantic Forest and Cerrado biomes were replaced by pasture. IBIS experiments showed that over the area of larger LULCC there was an increase in albedo of up to 8% while reductions in net radiation, surface heat fluxes, surface temperature and surface roughness were noticed. Land–atmosphere feedbacks assessed with BAM experiments showed that LULCC contributed to a drier and colder (stable) atmosphere over central‐east SA that opposes the necessary conditions for the rainy season onset. The changes included increases in surface pressure and low‐level wind associated with reductions in 2 m temperature and surface roughness. These changes contributed to decrease the cloud formation and less precipitation in all three rainy season onset years, but particularly in the late rainy season onset year during September–October–November (SON). The atmospheric changes induced by LULCC show a pattern of dry atmosphere (reduced precipitation), similar to those of late onset years, and indicate that LULCC enhances the late rainy season onset condition over central‐east SA. Additionally, this study highlights the importance of considering up‐to‐date vegetation maps because these changes significantly affect both surface and atmospheric variables.

Endemism and regionalization of Neotropical mammals: a multi-taxon analysis

Abstract The distribution pattern of a taxon is a consequence of historical and contemporary processes—specifically ecological and evolutionary processes—which tend to result in distributional congruence among species that respond similarly to the same processes. This congruence leads to the establishment of areas of endemism that are important for delimiting biogeographic regions. In the Neotropical region, different definitions of area of endemism have been proposed based on the distributional patterns of mammal species, each according to different methods and assumptions. In this study, we aimed to identify areas of endemism of Neotropical mammals, represented by 361 species of Placentalia (Xenarthra, Chiroptera, and Carnivora) and Marsupialia (Didelphimorphia and Paucituberculata), using an endemicity analysis implemented by the software NDM (eNDeMism software) and a hierarchical cluster analysis based on Simpson’s beta dissimilarity metric (βsim). The results suggest alternative spatial configurations within northern and southern parts of the Neotropics along transition zones. Additionally, there are indications of more restricted areas of endemism throughout the Neotropical region and with more species supporting the areas, such 1 area located in the region corresponding to the Atlantic forest and 2 areas corresponding to Mexican transition zone. The study enhances our understanding of Neotropical mammal biogeography and underscores the importance of using primary distribution data. Previous studies found 82 endemic species based on range maps, whereas our analyses identified 172 endemic species, including 125 that are newly proposed as such, and also confirmed the existence of 47 previously reported endemic species.

Mammal surveys and vertical strata use in an Atlantic Forest patch in the Pernambuco Endemism Center, Northeastern Brazil

Surveys are a key tool for the assessment of conservation strategies. We evaluated the relative abundance and vertical strata used by mammals in an Atlantic Forest patch in Igarassu (PE), Northeastern Brazil. We walked a total of 145.25 km on transects from December 2018 to June 2019, recording 105 sightings of eight species. Callithrix jacchus presented the highest relative abundance and Cuniculus paca the lowest. There was variation in the forest strata used by mammals. The records of rare and large-bodied mammals indicate the importance of this area for the maintenance of the mammalian fauna of the Pernambuco Endemism Center.

The Atlantic Forest Biome for a Vital synergism – A Symposium in Bahia, Where Stingless Bee Keeping Was Born in Brazil

The Atlantic Forest Biome for a Vital synergism – A Symposium in Bahia, Where Stingless Bee Keeping Was Born in Brazil

Vanilla from Brazilian Atlantic Forest: In vitro and in silico toxicity assessment and high-resolution metabolomic analysis of Vanilla spp. ethanolic extracts

The natural vanilla market, which generates millions annually, is predominantly dependent on Vanilla planifolia, a species characterized by low genetic variability and susceptibility to pathogens. There is an increasing demand for natural vanilla, prized for its complex, authentic, and superior quality compared to artificial counterparts. Therefore, there is a necessity for innovative production alternatives to ensure a consistent and stable supply of vanilla flavors. In this context, vanilla crop wild relatives (WRs) emerge as promising natural sources of the spice. However, these novel species must undergo toxicity assessments to evaluate potential risks and ensure safety for consumption. This study aimed to assess the non-mutagenic and non-carcinogenic properties of ethanolic extracts from V. bahiana, V. chamissonis, V. cribbiana, and V. planifolia through integrated metabolomic profiling, in vitro toxicity assays, and in silico analyses. The integrated approach of metabolomics, in vitro assays, and in silico analyses has highlighted the need for further safety assessments of Vanilla cribbiana ethanolic extract. While the extracts of V. bahiana, V. chamissonis, and V. planifolia generally demonstrated non-mutagenic properties in the Ames assay, V. cribbiana exhibited mutagenicity at high concentrations (5000 μg/plate) in the TA98 strain without metabolic activation. This finding, coupled with the dose-dependent cytotoxicity observed in WST-1 (Water Soluble Tetrazolium) assays, a colorimetric method that assesses the viability of cells exposed to a test substance, underscores the importance of concentration in the safety evaluation of these extracts. Kaempferol and pyrogallol, identified with higher intensity in V. cribbiana, are potential candidates for in vitro mutagenicity. Although the results are not conclusive, they suggest the safety of these extracts at low concentrations. This study emphasizes the value of an integrated approach in providing a nuanced understanding of the safety profiles of natural products, advocating for cautious use and further research into V. cribbiana mutagenicity.

Perceptions about the Atlantic Forest of basic education students in Santa Cruz Cabrália (BA, Brazil)

Perceptions about the Atlantic Forest of basic education students in Santa Cruz Cabrália (BA, Brazil)

Artisanal fishing affects the local distribution of broad‐snouted caiman (Caiman latirostris) within the Atlantic Forest of Brazil

Abstract Artisanal fishing is an important subsistence practice in freshwater habitats worldwide, but overexploitation threatens the conservation of several nontarget species including crocodylians. We investigated the effects of artisanal fishing on the distribution of a population of broad‐snouted caiman (Caiman latirostris) inhabiting the Tapacurá reservoir, within the highly altered and threatened Atlantic Forest biome. We conducted spotlight surveys to detect caimans and gillnets deployed in the reservoir from April 2015 to June 2022. We evaluated temporal differences in gillnet encounter rates and the relationship between caimans and gillnet distribution. Gillnet encounter rates remained consistent year‐round, while caiman encounter rates were highest near gillnets, especially in the river channel and in forested margins. Caimans are opportunistic predators attracted by tangled fish in gillnets and likely prefer habitats with increased fish abundance. Future research should continue monitoring the interaction between caimans and fishing and include local communities in conservation efforts.

Inundation dynamics in seasonally dry floodplain forests in southeastern Brazil

AbstractFloodplains are one of the most threatened ecosystems. Even though the vegetation composition in floodplain forests is expected to reflect the variation in groundwater levels and flood duration and frequency, there is little field data on the inundation dynamics (e.g., the variability in flood duration and flood frequency), especially for the understudied seasonally dry tropics. This limits our understanding of these ecosystems and the mechanisms that cause the flooding. We, therefore, investigated six floodplain forests in the state of Minas Gerais in Brazil for 1.5 years (two wet seasons): Capivari, Jacaré, and Aiuruoca in the Rio Grande basin, and Jequitaí, Verde Grande, and Carinhanha in the São Francisco basin. These locations span a range of climates (humid subtropical to seasonal tropical) and biomes (Atlantic forest to Caatinga). At each location, we continuously measured water levels in five geomorphologically distinct eco‐units: marginal levee, lower terrace, higher terrace, lower plain, and higher plain, providing a unique hydrological dataset for these understudied regions. The levees and terraces were flooded for longer periods than the plains. Inundation of the terraces lasted around 40 days per year. The levees in the Rio Grande basin were flooded for shorter durations. In the São Francisco basin, the flooding of the levees lasted longer and the water level regime of the levees was more similar to that of the terraces. In the Rio Grande basin, flooding was most likely caused by rising groundwater levels (i.e., “flow pulse”) and flood pulses that caused overbank flooding. In the São Francisco basin, inundation was most likely caused by overbank flooding (i.e., “flood pulse”). These findings highlight the large variation in inundation dynamics across floodplain forests and are relevant to predict the impacts of changes in the flood regime due to climate change and other anthropogenic changes on floodplain forest functioning.

Labor of a free-living buffy-tufted marmoset (Callithrix aurita) at a Wildlife Rehabilitation Center in southeastern Brazil.

Callithrix aurita is an endangered small primate endemic to the Atlantic Forest. The present work reports the labor of a free-living C. aurita, through observation of its length and offspring viability. A conservative treatment was used to maintain fetal viability, in view of the species conservation importance.

Subcutaneous occurrence of encysted Acanthocephala in an anuran amphibian from Brazil.

Acanthocephalans, in their adult stage, are obligatory parasites of many types of vertebrates, including anuran amphibians. Their complex life cycle always involves an arthropod intermediate host but may include non-obligatory strategies that could improve transmission success, such as paratenic infections. In paratenic hosts, these parasites are normally found loose in the body cavity or encysted in internal organs. Here, we present the first report of acanthocephalans found encysted under the skin of an amphibian (i.e., external to its body cavity). The specimen, a clay robber frog [Haddadus binotatus (Spix, 1824)], had been collected in an Atlantic Forest area in southeastern Brazil. Upon examination of the frog, we recovered two specimens of acanthocephalan (Order Echinorhynchida) encysted under the skin of its venter. Considering the host's relatively small size and its thin ventral musculature, we believe that the acanthocephalans may have accidentally trespassed the muscular tissue while attempting to encyst in the frog's internal body wall.

Does the body size, sex, and reproductive modes of leaf litter anurans affect the diversity of parasites?

We describe the composition of endoparasites associated with leaf litter anurans from an Atlantic Forest area, in southeastern Brazil. We tested if body size, sex, and reproductive modes of anuran hosts influence endoparasite abundance and richness. We sampled 583 individuals from 11 anuran species and recorded 1,600 helminths from 14 taxa. The helminths that infected the greatest number of anuran host species were the nematodes Cosmocerca parva (8 spp.), Physaloptera sp. (8 spp.), and Cosmocerca brasiliense (7 spp.), and the most abundant helminth species were Physaloptera sp. (14.6%), Cosmocerca brasiliense (13.7%) and Cosmocerca parva (12.6%). Both helminth abundance and richness were positively affected by anuran body size and dependence on water for reproduction. Larger hosts can contain a higher abundance of parasites because they may provide more physical space than smaller ones, or it can simply be a function of age. Besides, parasite species richness can be highly correlated with the amount of time a host spends in association with aquatic habitats, a conservative aspect of both parasite and host natural history. Within host species, there was a positive and significant influence of body size on helminth abundance. Haddadus binotatus females had greater helminth abundance than males, probably due to sex-related differences in behavior and/or in physiology. Our data suggest that reproductive modes could also influence helminth infection parameters in other anuran communities and should be considered in detail in future analyses.

Home Sweet Home: Yeasts Living in Substrates Related with Melipona scutellaris in the Brazilian Atlantic Forest

Home Sweet Home: Yeasts Living in Substrates Related with Melipona scutellaris in the Brazilian Atlantic Forest

Eugenia (Myrtaceae) from Reserva Natural Vale, Espírito Santo, a center of plant endemism in the Brazilian Atlantic Rainforest

Eugenia includes ca. 1,100 species worldwide and is the richest angiosperm genus of Brazilian flora with a remarkable biodiversity in the Atlantic Rainforest. In Espírito Santo state, the central-north region conserves the biggest forest fragment and is composed by the lowland coastal forests known as Tabuleiro. Considering the scarcity of studies approaching this vegetation and the high diversity of Eugenia species, this work presents a taxonomic treatment of Eugenia species from Reserva Natural Vale. The study area is located at the municipalities of Linhares and Sooretama, in the northern part of the coastal mesoregion of the state. For the taxonomic treatment, fieldwork was carried out between August 2015 and March 2019 and herbarium collections of BHCB, CVRD, CEPEC, ESA, HRCB, HPL, HUFSJ, ICN, K, MBM, MBML, RB, RBR, SORO, SP, SPF, SPSF, UB, UEC, and VIES were consulted, as well as images of nomenclatural types available online. This work points out the occurrence of 48 species of Eugenia within Reserva Natural Vale, encompassing 48% of the Eugenia species recorded in the Espírito Santo state. Six of them are presented as new occurrences for the reserve (Eugenia arenaria, E. disperma, E. florida, E. handroi, E. pachnantha and E. schottiana); two (E. pachnantha and E. schottiana) are first recorded for the state; E. adenophylla and E. infuscata are described as new. Eugenia adenophylla, E. atlantica, Eugenia cataphyllea, E. flavicarpa, E. hispidiflora, E. ochracea, E. penduliflora, and E. soteriana are endemic to Tabuleiro coastal forest, and E. handroi is the only record for Espírito Santo state. The most useful characters to distinguish the species are hair colours, especially in the floral structures, midvein on the adaxial surface of leaf blades, inflorescence type, connation and persistence of bracteoles, and the presence or absence of indumentum on the flowers’ hypanthia. Furthermore, morphological descriptions, distribution and phenology data, taxonomic comments, species illustrations, and an identification key for the species in the reserve are presented.

Potential of integrated trees-pasture-based systems for GHG emission mitigation and improving soil carbon dynamics in the Atlantic forest biome, Southeastern of Brazil

Brazilian cattle production is mainly carried out in pastures, and mitigating the livestock's greenhouse gas (GHG) emissions has become an essential prerequisite. The importance of planted forests in beef cattle pasture-based integrated systems has been widely recognized in the context of GHG emission mitigation. Adopting the intensification and integration with forest and crops of pasture-based livestock production systems are alternatives to optimizing the soil and tree carbon (C) sequestration; however, research on tropical conditions is still necessary. This long-term trial evaluated the potential of C sequestration (soil + trees) of the forest and the grazing systems with two replicate areas of 3 ha each: 1. continuous stocking rate (CLS) – pasture under continuous stocking; 2. agropastoral or crop-livestock system (AGROP) – pasture under rotational stocking in rotation with corn; 3. agrosilvipastoral or crop-livestock-forestry system (AGROSIL) - similar to AGROP but including eucalyptus; 4. silvipastoral or livestock-forestry system (SIL) - pasture under rotational stocking + eucalyptus and 5. Atlantic Forest (FOR). Soil samples were analysed for texture, C and bulk density in eight soil layers from three to eight trenches for each treatment, then C stock and C accumulation rates were calculated. Data from Eucalyptus urograndis of the SIL and AGROSIL systems with 333 trees. ha−1 during five first years and 166 trees. ha−1 in subsequent years were collected (five- and eight-years old trees) and used to define the wood volume and acquire wood rings. Elemental analyzer CHN performed soil and trees C content. Equations for determining stem volume and tree biomass and, afterward, the C pools of the tree trunks were proposed. The potential of GHG emission mitigation (t CO2 e.. ha−1. year−1) by annual C sequestration (soil or soil + trees) was calculated, including the wood used for the lumber-mill scenario. The participation of the C stock from the 0–30 cm soil layer was between 45 % and 53 % of the total C stock in the 0–100 cm soil layer. Regardless of the elevated C soil accumulation rates, 0.88, 2.54, and 1.74 t C. ha-1. year−1 in the conversion stages CLS → AGROP; AGROP → SIL; AGROP → AGROSIL, respectively, it was not possible to compensate for the negative result of the Forest → CLS conversion (-2.55 t C. ha-1. year−1) because this stage lasted 12 years, while the implementation of AGROSIL and SIL systems had only six years. However, when C tree accumulation rates were considered, the potential of GHG emission mitigation (t CO2 e. ha−1. year−1) by annual C sequestration (soil +trees) was 19.89 to AGROSIL and 18.97 to SIL, including the wood used for the lumber-mill scenario. Based on these results, it can be concluded that C sequestration in trees-pasture integrated systems occurs in layers deeper, with the diverse species contributing in different soil layers; there is a positive effect of improved land-use management, with the use of integrated systems on annual C soil sequestration and the trees consists of an important strategy to improve the GHG mitigation.

Long-Term Trends in Mosquito Vector Populations and Their Impact on Yellow Fever Outbreaks in Atlantic Forest Fragments of Rio De Janeiro, Brazil (2016-2021).

Among all living beings, mosquitoes account for the highest number of human fatalities. Our study aimed to determine mosquito egg abundance fluctuation from 2015 to 2020, in order to observe which years had the highest mosquito vector densities and whether they coincided with yellow fever virus outbreaks in both human and nonhuman primates. The study area included Atlantic Forest fragments in the state of Rio de Janeiro. Studies from the Diptera Laboratory at FIOCRUZ were selected and compared along a timeline period of the field collections. The highest peak in egg abundance from the analyzed studies was observed from 2016 to 2017 and from 2015 to 2016. The lowest egg abundance was during the collection periods from 2018 to 2019 and 2019 to 2020. The species with the highest abundance throughout all the periods of the studies analyzed was Haemagogus leucocelaenus, representing 87% of all epidemiological species identified. The species with the lowest abundance was Hg. Janthinomys, representing only 1%. Monitoring the population of mosquitoes is imperative for disease surveillance, as the rise in specimens of various vector species directly impacts the occurrence of yellow fever cases in both nonhuman primates and human populations.

Guiding seed movement: environmental heterogeneity drives genetic differentiation in Plathymenia reticulata, providing insights for restoration.

Forest and landscape restoration is one of the main strategies for overcoming the environmental crisis. This activity is particularly relevant for biodiversity-rich areas threatened by deforestation, such as tropical forests. Efficient long-term restoration requires understanding the composition and genetic structure of native populations, as well as the factors that influence these genetic components. This is because these populations serve as the seed sources and, therefore, the gene reservoirs for areas under restoration. In the present study, we investigated the influence of environmental, climatic and spatial distance factors on the genetic patterns of Plathymenia reticulata, aiming to support seed translocation strategies for restoration areas. We collected plant samples from nine populations of P. reticulata in the state of Bahia, Brazil, located in areas of Atlantic Forest and Savanna, across four climatic types, and genotyped them using nine nuclear and three chloroplast microsatellite markers. The populations of P. reticulata evaluated generally showed low to moderate genotypic variability and low haplotypic diversity. The populations within the Savanna phytophysiognomy showed values above average for six of the eight evaluated genetic diversity parameters. Using this classification based on phytophysiognomy demonstrated a high predictive power for genetic differentiation in P. reticulata. Furthermore, the interplay of climate, soil and geographic distance influenced the spread of alleles across the landscape. Based on our findings, we propose seed translocation, taking into account the biome, with restricted use of seed sources acquired or collected from the same environment as the areas to be restored (Savanna or Atlantic Forest).

Begonia isadorae (Begoniaceae), a new endemic species from Ilha Grande, an island in southeastern Brazil, with notes on leaf anatomy

Begonia isadorae is a new species endemic to an island of Brazilian Atlantic Rainforest, a biome rich in endemism and species diversity, and it is described and illustrated herein. Begonia isadorae belongs to B. sect Pritzelia (Klotzsch) A.DC. and it is morphologically similar to B. rubropilosa A.DC. sharing the same leaf blade shape and habit but differs in its puberulous to glabrescent adaxial leaf blades (vs. scabrous), puberulous tepals (vs. squamulose), and ovary with entire placentae (vs. bilamellate). A diagnosis, description, ecological comments, taxonomic and leaf anatomy notes, illustrations, and color photographs are provided. Begonia isadorae is preliminarily classified as Vulnerable (VU) due to its very small populations and restricted distribution.

Applicability of Traps for Collecting Mosquito Immatures (Diptera: Culicidae) for Entomological Surveillance of Arbovirus Vectors in a Remnant of the Atlantic Forest, Rio de Janeiro State, Brazil.

Diverse larval habitats significantly influence female mosquito oviposition. Utilizing traps that simulate these habitats is helpful in the study of the bioecology and characteristics of pathogen-transmitting species during oviposition. This study evaluated the feasibility of different traps in natural environments by comparing sampling methods and detecting the oviposition of epidemiologically important mosquitoes, with emphasis on Haemagogus species, in a fragment of the Atlantic Forest in Silva Jardim, Rio de Janeiro State, Brazil. Monthly collections were conducted from March 2021 to October 2023 using four types of traps: plastic containers, tires, bamboo, and sapucaia. Immatures were collected from these traps using a pipette, placed in plastic bags, and transported to the laboratory. Tire was the most efficient trap, showing the highest mosquito abundance (n = 1239) and number of species (S = 11). Conversely, the plastic container trap exhibited the lowest diversity (H = 0.43), with only two species and a low mosquito abundance (n = 26). The bamboo trap captured six species and recorded the second-highest diversity index (H = 1.04), while the sapucaia trap captured five species and had the third-highest diversity index (H = 0.91). Of the total immatures collected, 1817 reached adulthood, comprising 13 species, two of which are vectors of the sylvatic yellow fever virus: Haemagogus leucocelaenus and Haemagogus janthinomys. In conclusion, detecting key vectors of the sylvatic yellow fever virus in Brazil highlights the need for ongoing entomological and epidemiological surveillance in the study area and its vicinity. These efforts are crucial for monitoring vector presence and activity, identifying potential transmission hotspots, and devising effective control and prevention strategies.