During the competitive activity of football players, maintaining a high level of energy supply for ensuring muscle activity and special physical performance is a priority issue. Therefore, achieving and maintaining a high level of physical performance is an important factor that ensures the result. The purpose of the research is to study the effect of interval training method on speed endurance of football players. The following research methods were used: analysis of scientific and methodological literature, pedagogical scientific experiment, mathematical statistics. To achieve the set goals, particularly within the framework of improving speed endurance indicators, the interval training method was chosen for comparison with small-sided games in a square as part of the research. The research was carried out in 2022, in January-February, at Football Academy of Gyumri. 28 football players of "Shirak" and "Shirak-2" aged from 17 to 20 participated in the research: 14 athletes in experimental group and 14 in control group. Research result analysis: In the training system for football players, practically every session involves simultaneous work in multiple power zones, where quick movements occur in both maximum and submaximal zones. This, in turn, contributes to the reduction of both creatine phosphate and glycogen reserves in the muscles. To ensure the proper level of recovery for various energy substrates, it is necessary to carefully select methods and means to achieve the set goals, promoting the growth of both the organism functional capabilities and the concentration of macro ergonomic compounds. This is aimed at increasing speed-strength performance and endurance. During the 6 weeks, the football players trained five times a week, using the interval method once a week in the first, third and sixth weeks and twice in the second, fourth and fifth weeks, respectively. As an interval method 8 times 35m running at maximum speed with 1.5 minute rest intervals (slow running) was used in the experimental group. The control group performed a two-minute game in a square (12x12) 5 times for 30 seconds with intervals. The results obtained during the research reveal that there were significant differences in the indicators in both the experimental and control groups, but the increase in the indicators in the experimental group was more vivid: 4.23% and 2.16%, respectively. Like the interval method, the game in the square contributes to the improvement of the speed endurance of the football players. Along with that, it was revealed that the use of the interval method is more effective for developing the speed endurance of football players. A trend of significant difference between groups was recorded (р <0.05).