Ehrlichia canis and Anaplasma phagocytophilum have been reported to cause disease in animals and humans. In cats, the first evidence for naturally occurring ehrlichiosis was reported in France in 1986. Since then, several clinical cases of feline ehrlichiosis have been described worldwide and there is molecular and serological evidence of exposure to these agents in cats. However, no Ehrlichia species. has been cultured from the blood of these animals and information available about attempts to isolate these agents in cats is limited. In Spain, different studies on feline ehrlichiosis and anaplasmosis have shown seroprevalences ranging from 1.8% to 4.9% for A. phagocytophilum [1,2], and from 10.6% to 17.9% for E. canis [2,3]. Although Ehrlichia ⁄Anaplasma spp. sequence has not been obtained yet from cats, DNA from these species has been amplified from a sample from a cat from the Barcelona area [4]. To date, E. canis and A. phagocytophilum isolates from cats have not been characterised in the geographic area of Madrid (central Spain).