Assiut Governorate Research Articles

Seroprevalence of brucellosis among cows and sheep in Assiut province: A pilot study

Brucellosis is a common neglected zoonotic disease with a global geographic distribution that endangers human health and animal production. However, there is a scarcity of studies focusing on brucellosis in neglected regions such as Assiut governorate in Egypt. Therefore, the objective of this study was to investigate the prevalence of antibodies reactive to Brucella sp. in sheep and cows, as well as the associated risk factors, in Assiut governorate. A total of 184 sheep and 166 cows were included in the study, and their serum samples were subjected to screening using the Rose-Bengal plate test, followed by confirmation through the serum tube agglutination test. The overall seroprevalence of Brucella antibodies in sheep of 8.2% (15/184), while in cows, the seroprevalence was 1.2% (2/166). Among sheep, the seropositivity for Brucella abortus was 4.3% (8/184), whereas for Brucella melitensis, it was 6% (11/184). In cows, one cow displayed seropositivity for Brucella abortus, while the other exhibited mixed infection with both Brucella abortus and Brucella melitensis. Moreover, animals originating from villages bordering Sohag governorate demonstrated a higher risk of contracting brucellosis while other risk factors did not impact the occurrence of brucellosis. In conclusion, this study provides compelling evidence of the endemic nature of brucellosis in the investigated areas, with a particularly high prevalence observed in sheep. Both cows and sheep are susceptible to infection by either Brucella abortus or Brucella melitensis. To gain a comprehensive understanding of the epidemiological factors associated with infection, it is imperative to conduct large-scale surveys that incorporate molecular isolation of various Brucella species to facilitate the development of appropriate prevention strategies.

Pattern of Family Planning in Bani-Talib village in Assiut, Egypt-cross Sectional Study

Family planning programs have emerged as one of the most successful public health interventions of the 20th century, significantly improving maternal and child health outcomes. This study aims to evaluate the prevalence and patterns of contraceptive use in Bani-Talib village, Assiut governorate, and to identify barriers to family planning method utilization. Conducted as part of a baseline survey by the Ministry of Health and Population in 2010, this cross-sectional study included all married women aged 15-49 in the village. Data were collected through structured questionnaires addressing demographic information and contraceptive practices. Ethical approval was obtained, and data were analyzed using SPSS. The mean age of participants was 30.38 years, with an illiteracy rate of 63.8% and only 4.6% employed. The prevalence of contraceptive use was 46%, with 36.9% never having used any method. The most commonly used contraceptive method was the intrauterine device (IUD) at 40.4%, followed by injectables (32%) and oral contraceptive pills (22.1%). Major barriers to contraceptive use included newly married status (34.9%), cost (24.8%), and concerns about infertility (20.2%). The findings revealed no significant differences in contraceptive use related to education, employment status, or number of children, but age was significantly associated with usage patterns. The results highlight the need for targeted interventions to address barriers to contraceptive use and increase awareness of family planning among men. Future studies should further explore family planning utilization patterns in urban versus rural settings in Egypt. Enhanced training for healthcare providers is also recommended to improve family planning service delivery in rural communities.

Evaluation of multiplex PCR assay against microscopy in diagnosing of Entamoeba stool samples at Assiut Governorate Hospitals, Egypt

Evaluation of multiplex PCR assay against microscopy in diagnosing of Entamoeba stool samples at Assiut Governorate Hospitals, Egypt

The Impact of Climate Change on Rural Life Aspects Frome The Perspective of Rural Women in Assiut Governorate

The Impact of Climate Change on Rural Life Aspects Frome The Perspective of Rural Women in Assiut Governorate

The Role of Civil Society Organizations in Solving Some the Rural Social Problems in Assiut Governorate

The Role of Civil Society Organizations in Solving Some the Rural Social Problems in Assiut Governorate

Behavior of Anise farmers in Assiut Governorate

Behavior of Anise farmers in Assiut Governorate

Wheat food security indicators under improved irrigation conditions in Assiut Governorate

Wheat food security indicators under improved irrigation conditions in Assiut Governorate

Social Factors Affecting the Population Problem in Rural Assiut Governorate

Social Factors Affecting the Population Problem in Rural Assiut Governorate

Women Participation in The Environment Conservation in Rural Assiut Governorate

Women Participation in The Environment Conservation in Rural Assiut Governorate

Assessing the Relationships among Rural Organizations Working Within Decent Life Initiative in Assiut Governorate using the Graph-Theoretic Technique

Assessing the Relationships among Rural Organizations Working Within Decent Life Initiative in Assiut Governorate using the Graph-Theoretic Technique

Effectiveness of some plant extracts in biocontrol of induced onion basal rot disease in greenhouse conditions

One of Egypt’s most notable and historically significant vegetable crops is the Liliaceae plant, Allium cepa L. In this study, the effectiveness of methanolic extracts of Artemisia absinthium leaves, Calotropis procera latex, Moringa oleifera seeds, and Syzygium aromaticum clove was investigated in vitro and, in a greenhouse, setting against Fusarium oxysporum, the pathogen that causes onion basal rot in Assiut Governorate, Egypt. The S. aromaticum extract exhibited the inhibition peak (63.3%), whereas the A. absinthium extract had the lowest inhibition impact against F. oxysporum growth (41.1%). The gas chromatography-mass spectroscopy (GC–MS) analysis revealed that 82 important compounds, with abundances ranging from low to high, were present in the tested S. aromaticum’s methanolic extract. The primary components were acetaldehyde, hydroxy- and 2-propanone, 1,1,3,3-tetrachloro-(42.71%), 1,2-ethanediol, and methyl alcohol (34.01%). In comparison to the infected control, the disease severity was significantly reduced by 20% with the use of a plant extracts mixture and Dovex 50% and increased by 62.22% with the use of an extract from A. absinthium. When compared to the infected control, onion plant fresh weight and dry weight were considerably higher under the clove extract therapy. The plant extracts used in this study’s testing contain a number of active ingredients, including amino acids, vitamins, minerals, antioxidants, and enzymes, which is probably why they have such positive impacts. The application of a combination of plant extracts was suggested as a feasible strategy for improving the growth and productivity of onion plants by the study’s findings. More research is needed to comprehend the mechanisms by which plant extracts promote plant development and to optimize the concentration and timing of administration.

Postpartum family planning among women attending maternal and child health centers in Assiut Governorate, Upper Egypt

BackgroundPostpartum family planning (PPFP) is important in helping couples to achieve their reproductive intentions. National surveys have consistently reported higher fertility, lower use of family planning (FP), and higher unmet needs for FP in Upper Egypt. This study aims to identify the factors associated with the use of PPFP in Assiut Governorate, Upper Egypt, and to assess the current status of integration of PPFP counseling in the existing maternal and child health services (MCH).MethodsThe study employed a cross-sectional study design, collecting data from 455 postpartum women aged 15 to 49 years in 4 primary health care (PHC) centers in Assiut Governorate, Upper Egypt. The women were interviewed using a structured questionnaire. The questionnaire consisted of 4 sections: the first section included the participants’ demographic characteristics; the second section included women’s obstetric history; the third section included questions about PPFP knowledge, attitude, past and current use of contraception; and the fourth section assessed the current status of integrating PPFP counseling within antenatal, natal, and postnatal services.ResultsIn total, 54.5% of postpartum women were using a modern contraceptive method. The significant predictors of using PPFP methods were as follows: receiving information about PPFP from health care providers (AOR = 11.46, p < 0.001), better attitude towards PPFP (AOR = 10.54, p < 0.001), using modern FP methods (AOR = 6.98, p < 0.001), resumption of menstruation (AOR = 4.11, p < 0.001), older age (AOR = 2.15, p < 0.05), and better PPFP knowledge (AOR = 1.72, p < 0.001). Only 5.3%, 1.3%, and 3.5% received PPFP counseling during antenatal care (ANC), delivery, and the postpartum period, respectively.ConclusionsPostpartum contraception use was associated with receiving PPFP counseling by health care providers and women’s knowledge and attitude regarding PPFP. However, FP counseling was not integrated with other MCH services. Updating the components of MCH services to include PPFP counseling during ANC, at delivery, and during the postpartum period should be prioritized by program planners and policymakers.

Farmers' knowledge level of extension recommendations for onion diseases control in Assiut governorate, Egypt

Farmers' knowledge level of extension recommendations for onion diseases control in Assiut governorate, Egypt

Breeders’ Perception of the Risks of the Climate Change Effects on Livestock and ways to confront it in Assiut Governorate

Breeders’ Perception of the Risks of the Climate Change Effects on Livestock and ways to confront it in Assiut Governorate

Evaluation of Aflatoxin M1 in raw buffalo milk and some dairy products in Assiut governorate, Egypt

Evaluation of Aflatoxin M1 in raw buffalo milk and some dairy products in Assiut governorate, Egypt

Vaccines defiance against SARS COV-2 variants at Assiut Governorate

Vaccines defiance against SARS COV-2 variants at Assiut Governorate

Mediating Role of Customer Relationship Quality between the Customer Value Co-creation Behavior and Customer Loyalty

The purpose of this study was to determine the role of customer relationship quality in mediating the relationship between customer value co-creation behavior and customer loyalty in Egyptian private hospitals and medical centers in Assiut Governorate. The descriptive analytical method was used in the study. 445 of the 500 patients in the sample were collected by a questionnaire, which was valid for statistical analysis. The study’s findings supported the mediating role of customer relationship quality in the relationship between customer value co-creation behavior and customer loyalty. Implications, recommendations, and potential future studies in the subject of study were discussed.

Histological, immunohistochemical and serological investigations of the ovary during follicular phase of estrous cycle in Saidi sheep

BackgroundSaidi sheep are the most abundant ruminant livestock species in Upper Egypt, especially in the Assiut governorate. Sheep are one of the most abundant animals raised for food in Egypt. They can convert low-quality roughages into meat and milk in addition to producing fiber and hides therefore; great opportunity exists to enhance their reproduction. Saidi breed is poorly known in terms of reproduction. So this work was done to give more information on some hormonal, oxidative, and blood metabolites parameters in addition to histological, histochemical and immunohistochemical investigations of the ovary during follicular phase of estrous cycle. The present study was conducted on 25 healthy Saidi ewes for serum analysis and 10 healthy ewes for histological assessment aged 2 to 5 years and weighted (38.5 ± 2.03 kg).ResultsThe follicular phase of estrous cycle in Saidi sheep was characterized by the presence of ovarian follicles in different stages of development and atresia in addition to regressed corpus luteum. Interestingly, apoptosis and tissue oxidative markers play a crucial role in follicular and corpus luteum regression. The most prominent features of the follicular phase were the presence of mature antral (Graafian) and preovulatory follicles as well as increased level of some blood metabolites and oxidative markers. Here we give a new schematic sequence of ovarian follicles in Saidi sheep and describing the features of different types. We also clarified that these histological pictures of the ovary was influenced by hormonal, oxidative and blood metabolites factors that characterizes the follicular phase of estrous cycle in Saidi sheep.ConclusionThis work helps to understanding the reproduction in Saidi sheep which assist in improving the reproductive outcome of this breed of sheep. These findings are increasingly important for implementation of a genetic improvement program and utilizing the advanced reproductive techniques as estrous synchronization, artificial insemination and embryo transfer.

Determination the Presence of Antibiotics Residues in Raw Buffalo Milk in Assiut Governorate, Egypt

Determination the Presence of Antibiotics Residues in Raw Buffalo Milk in Assiut Governorate, Egypt

Microscopic and molecular detection of piroplasms among sheep in Upper Egypt.

Blood parasites pose a significant threat to livestock production in southern Egypt, yet there is a scarcity of information regarding their circulation and epidemiology in sheep in this region. This study aimed to investigate the seroprevalence of blood parasite infections in sheep in Assiut governorate, Upper Egypt. A total of 400 blood samples were collected from sheep of varying ages and genders. The preliminary screening for the presence of piroplasms, mainly Babesia and Theileria spp., via microscopic examination, followed by investigation of the potential risk factors linked with the exposure to infection. Moreover, molecular identification of both parasites on some of positive samples was performed using PCR targeting Babesia 18S rRNA and Theileria annulata Tams1 gene. The microscopic examination revealed that among the examined sheep, there was an overall prevalence of blood parasites at 44% (176 out of 400), with Babesia spp. observed in 14% (56 out of 400) and Theileria spp. in 30% (120 out of 400). Furthermore, the infection rate was non-significantly higher in young animals (50%) compared to adults (38.5%) (P = 0.246). Male sheep exhibited a significantly higher vulnerability to both parasites' infection (63.3%) compared to females (35.7%) (P = 0.011). Interestingly, the prevalence of both blood parasites was significantly higher during the cold season (66.1%) compared to the hot season (15.9%) (P = < 0.001). The molecular analysis identified the presence of Babesia ovis and Theileria annulata among a subsample of the positive sheep's bloods films. The identified species were recorded in the GenBank™ databases and assigned specific accession numbers (OQ360720 and OQ360719 for B. ovis), and (OP991838 for T. annulata). Taken together, this study confirms a high prevalence of piroplasmosis and offers epidemiological and molecular insights into blood parasites in sheep from Upper Egypt, highlighting the importance of detecting these parasites in various hosts and their competent vectors (ticks).