Behavioral Assessment Research Articles

Targeting early tau pathology: probiotic diet enhances cognitive function and reduces inflammation in a preclinical Alzheimer’s model

BackgroundAlzheimer’s disease (AD) remains incurable, yet its long prodromal phase offers a crucial window for early intervention. Pretangle tau, a precursor to neurofibrillary tangles, plays a key role in early AD pathogenesis. Intervening in pretangle tau pathology could significantly delay the progression of AD. The gut-brain axis, increasingly recognized as a contributor to AD, represents a promising therapeutic target due to its role in regulating neuroinflammation and neurodegeneration. While probiotics have shown cognitive benefits in amyloid-centered AD models, their effect on pretangle tau pathology remains elusive.MethodsThis study evaluates the effects of probiotics in a rat model of preclinical AD, specifically targeting hyperphosphorylated pretangle tau in the locus coeruleus. TH-CRE rats (N = 47; 24 females and 23 males) received either AAV carrying pseudophosphorylated human tau (htauE14) or a control virus at 3 months of age. Probiotic or control diets were administered at 9–12 months, with blood and fecal samples collected for ELISA and 16S rRNA gene sequencing. Behavioral assessments were conducted at 13–14 months, followed by analysis of brain inflammation, blood-brain barrier integrity, and GSK-3β activation.ResultsRats expressing pseudophosphorylated tau displayed impairment in spatial Y-maze (F1,39 = 4.228, p = 0.046), spontaneous object location (F1,39 = 6.240, p = 0.017), and olfactory discrimination (F1,39 = 7.521, p = 0.009) tests. Phosphorylation of tau at S262 (t3 = -4.834) and S356 (t3 = -3.258) in the locus coeruleus was parallelled by GSK-3β activation in the hippocampus (F1,24 = 10.530, p = 0.003). Probiotic supplementation increased gut microbiome diversity (F1,31 = 8.065, p = 0.007) and improved bacterial composition (F1,31 = 3.4867, p = 0.001). The enhancement in gut microbiomes was associated with enhanced spatial learning (p < 0.05), reduced inflammation indexed by Iba-1 (F1,25 = 5.284, p = 0.030) and CD-68 (F1,26 = 8.441, p = 0.007) expression, and inhibited GSK-3β in female rats (p < 0.01 compared to control females).ConclusionsThis study underscores the potential of probiotics to modulate the gut-brain axis and mitigate pretangle tau-related pathology in preclinical AD. Probiotic supplementation could offer a novel early intervention strategy for AD, highlighting the pivotal role of gut health in neurodegeneration.

Moroccan Adaptation of the 'Self-Care Behaviour Assessment Scale' for Arteriovenous Fistula in Haemodialysis.

Arteriovenous fistula self-care behaviours in patients receiving haemodialysis are essential to maintain patency of vascular access and prevent its life-threatening complications. Assessing arteriovenous fistula self-care behaviours in patients receiving haemodialysis requires a reliable and valid tool. The aim of this study was to adapt and translate the Portuguese scale for the assessment of self-care behaviours of arteriovenous fistula in patients receiving haemodialysis into the Moroccan dialect and evaluate its psychometric properties in the Moroccan context. A quantitative cross-sectional validation study. This study included 283 patients undergoing haemodialysis with arteriovenous fistula in the Marrakech-Safi region of Morocco. The translation was performed in accordance with international guidelines. Instrument validity was assessed using content validity and factor analysis with Varimax rotation for construct validity. Instrument reliability was assessed by measuring internal consistency using Cronbach's α. Content validity was assessed by a panel of experts. The Kaiser-Meyer-Olkin score of 0.885 justified data adaptation for factorial analysis. Bartlett's sphericity test confirmed the existence of significant relationships (chi-square = 1347.328; p < 0.001). A two-factor structure was extracted, explaining 50.85% of the total variance. Cronbach's α was 0.892, 0.853 and 0.752 for the overall scale, the self-care subscale for managing signs and symptoms and the self-care subscale for preventing complications, respectively. The Moroccan version of the Self-Care Behaviour Assessment Scale for Arteriovenous Fistula is a reliable and valid instrument for future studies evaluating the self-care behaviours of patients receiving haemodialysis with an arteriovenous fistula in Morocco.

Application of stem cells alone and in combination with low-level laser therapy for sciatic nerve repair in rats

This study evaluated the efficacy of tubular constructs containing stem cells and Type I collagen, both independently and in conjunction with low-level laser therapy (LLLT), in repairing the sciatic nerve in a rat model. In this animal study, the right sciatic nerve of 30 male Wistar rats, each weighing 250–300 g, was surgically excised to a length of 8 mm. The rats were then randomly allocated to three groups (n = 10 per group). In Group 1, the excised nerve segment was utilized as an autograft and sutured at the defect site. In Group 2, a tubular construct containing stem cells and Type I collagen was used to bridge the proximal and distal ends of the nerve. Group 3 received the same intervention as group 2, supplemented with 5 weeks of LLLT. After 5 and 12 weeks, rats underwent histological, behavioral, and electrophysiological assessments. Data were statistically analyzed using analysis of variance (ANOVA), Bonferroni post-hoc test, and Kruskal–Wallis test. At both 5 and 12 weeks, axonal count and nerve repair scores showed no significant differences among the three groups (P > 0.05). Notably, the Sciatic Functional Index (SFI) was the most favorable (lowest) in the autograft group, whereas the stem cell-only group exhibited the least favorable (highest) SFI at 5 weeks (P < 0.001). Additionally, distal latency was highest in the stem cell group and lowest in the stem cell combined with LLLT group at 5 weeks (P < 0.001). A significant difference was observed between the autograft and stem cell plus LLLT groups (P < 0.05). In conclusion, the application of stem cell-laden tubular constructs in conjunction with LLLT demonstrated efficacy for sciatic nerve repair in rats.

Astrocytic Acid-Sensing Ion Channel 1a Contributes to the Development of Epileptic Cognitive Impairment

Reactive astrogliosis and acidosis, common features of epileptogenic lesions, express a high level of astrocytic acid-sensing ion channel-1a (ASIC1a), a proton-gated cation channel and key mediator of responses to neuronal injury. This study investigates the role of astrocytic ASIC1a in cognitive impairment following epilepsy. Status epilepticus (SE) in C57/BL6 mice was induced using lithium–pilocarpine; the impact of ASIC1a on astrocytes was assessed using rAAV–ASIC1a–NC and rAAV–ASIC1a–shRNA, injected in the CA3 region of mice. Behavioral assessments were conducted using the Morris water maze (MWM). Western blotting and immunofluorescence were applied to evaluate ASIC1a and Gfap expression while analyzing intracellular calcium and extracellular glutamate (Glu) concentrations in primary cultured astrocytes isolated from the brains of 1 to 3-day-old mice and treated LPS. Results showed enhanced astrocyte proliferation and ASIC1a expression in the dentate gyrus of epileptic mice 7, 21, and 28 days post-SE (all p &lt; 0.05). Escape latency in the MWM further suggested that ASIC1a regulates cognitive function in mice with chronic epilepsy. LPS stimulation in vitro mimicked inflammatory responses, increasing ASIC1a after 24 h, which increased the concentration of intracellular calcium and extracellular expression of Glu; inhibition of ASIC1a expression reversed this process. To sum up, these data confirm that astrocytic ASIC1a may facilitate cognitive dysfunction post-epilepsy, presenting a potential therapeutic target.

Longitudinal Assessment of Sexual Behavior and Relationship Quality During the First Year of the COVID-19 Pandemic in Britain: Findings from a Longitudinal Population Survey (Natsal-COVID)

ABSTRACT While the impact of social restrictions on sexual and romantic life early in the COVID-19 pandemic has been widely studied, little is known about impacts beyond the initial months. We analyzed responses from 2,098 British adults (aged 18–59) taking part in the Natsal-COVID study (Waves 1 and 2). Participants were recruited via a web panel and surveyed twice: four months and one year after the start of the UK’s first national lockdown (July 2020 and March 2021). Changes in the prevalence and frequency of participants’ physical and virtual sexual behaviors between the two surveys were analyzed using multinomial logistic regression. Changes in the quality of intimate relationships were modeled using logistic regression for the 1,407 participants in steady relationships, adjusting for age, gender, and relationship status. The reported prevalence of any sexual activity amongst the full sample increased over the study period (from 88.1% to 91.5%, aOR = 1.50, 95% CI 1.23–1.84). Increases were observed for physical (aOR = 1.41, 95% CI 1.15–1.74) and virtual (aOR = 1.20, 95% CI 1.07–1.34) activities, particularly masturbation (aOR 1.53, 95% CI 1.37–1.72). Increases were larger for men than women. The proportion of participants in steady relationships whose relationship scored as “lower quality” increased (from 23.9% to 26.9%, aOR = 1.28, 95% CI 1.10–1.49). These findings have implications for understanding sexual health needs during disasters and planning sexual health service priorities following the pandemic.

Eating behavior patterns, metabolic parameters and circulating oxytocin levels in patients with obesity: an exploratory study

PurposeObesity is a complex heterogeneous disease often associated with dysfunctional eating behavior patterns. Oxytocin (OT) is a neurohormone involved in the regulation of energy metabolism and eating behavior. The aim of the present study was to evaluate in a population of patients with obesity circulating levels of OT and dysfunctional eating behaviors in relation to anthropometric, hormonal and metabolic parameters.MethodsA prospective, observational, single-center study was conducted at the Center of High Specialization for the Care of Obesity of Sapienza University of Rome. Adult subjects with body mass index (BMI) ≥ 30 kg/m2 were recruited. Body impedance assessment (BIA), biochemical and hormonal parameters, plasma OT concentration analysis and the Eating Behaviors Assessment for Obesity (EBA-O) questionnaire were evaluated.ResultsA total of 21 patients, 16 females and 5 males, with a mean age of 45.7 ± 15.1 years, mean BMI of 40.89 ± 8.02 kg/m2 and plasma OT concentration of 1365.61 ± 438.03 pg/mL were recruited. The dysfunctional eating behavior traits investigated by the EBA-O appear significantly associated with metabolic derangements. In particular, night eating is associated with alterations in lipid metabolism (p < 0.01). Circulating OT correlates positively with BMI (r = 0,43; p < 0.05), and Hepatic Steatosis Index (HIS) (r = 0.46; p < 0.05), while its role in subjects with obesity and alterations in glucose metabolism is less clear. Interestingly, circulating OT levels < 1312.55 pg/mL may be predictive of food addiction (100% sensitivity; 62.5% specificity).ConclusionsDespite the need for larger studies to confirm their validity, the clinical utility of the EBA-O and circulating OT in identifying dysfunctional eating behaviors appears promising.

Optimizing Neurobehavioral Assessment for Patients with Disorders of Consciousness: Proposal of a Comprehensive Pre-Assessment Checklist for Clinicians

Background: Clinicians are challenged by the ambiguity and uncertainty in assessing level of consciousness in individuals with disorder of consciousness (DoC). There are numerous challenges to valid and reliable neurobehavioral assessment and classification of DoC due to multiple environmental and patient-related biases including behavioral fluctuation and confounding or co-occurring medical conditions. Addressing these biases could impact accuracy of assessment and is an important aspect of the DoC assessment process. Methods: A pre-assessment checklist was developed by a group of interdisciplinary DoC clinical experts and researchers based on the existing literature, current validated tools, and expert opinions. Once finalized, the checklist was electronically distributed to clinicians with a range of experience in neurobehavioral assessment with DoC. Respondents were asked to use the checklist prior to completing a neurobehavioral assessment. A survey was also provided to respondents to obtain feedback regarding checklist feasibility and utility in optimizing the behavioral assessments. Results: Thirty-three clinicians completed the survey after using the checklist. Over half of the respondents were a combination of physicians, neuropsychologists, and physical therapists. All respondents served the adult DoC population and 42% percent had over ten years of clinical experience. Eighty percent reported they found the format of the checklist useful and easy to use. All respondents reported the checklist was relevant to preparing for behavioral assessment in the DoC population. Eighty-four percent reported they would recommend the use of the tool to other clinicians. Conclusions: The use of a pre-assessment checklist was found to be feasible and efficacious in increasing interdisciplinary clinician’s ability to optimize the patient and environment in preparation for neurobehavioral assessment. Initial results of clinicians’ perception of the utility of a pre-assessment checklist were positive. However, further validation of the tool is needed with larger sample sizes to improve representation of clinical use across disciplines and care settings.

Cannabidiol abrogates cue-induced anxiety associated with normalization of mitochondria-specific transcripts and linoleic acid in the nucleus accumbens shell.

Anxiety disorders are one of the top contributors to psychiatric burden worldwide. Recent years have seen a dramatic rise in the potential anxiolytic properties ascribed to cannabidiol (CBD), a non-intoxicating constituent of the Cannabis Sativa plant. This has led to several clinical trials underway to examine the therapeutic potential of CBD for anxiety disorders. Yet, CBD's anxiolytic effects are mixed with some studies reporting little to no impact on trait anxiety but significant reductions in pathological anxiety with suggestions that CBD's effect may relate to triggered or cue-induced behavior. Here, we studied the effects of CBD on cued and non-cued behaviors and related neurobiological underpinnings. To investigate the effect of CBD on cue-induced anxiety, male rats underwent a fear conditioning protocol (odor associated with shock) followed by assessments of avoidance behavior. CBD (10 mg/kg) was administered 1 h prior to anxiety assessments. To understand molecular mechanisms associated with behavior, we investigated the transcriptome and lipid profile of the nucleus accumbens shell (NAcSh), a structure implicated in cue-mediated behaviors and aversion. Administration of CBD significantly reduced avoidance behavior, but only in animals repeatedly exposed to a shock-paired cue. CBD did not affect behavior in animals exposed to neutral cue or encoding of the cue behavioral response. RNA sequencing revealed substantial impact of the shock-paired cue in control animals, recruiting mechanisms ranging from cytoskeletal dynamics to mitochondria dysfunction. The shock-paired cue also resulted in elevated linoleic acid in vehicle animals which correlated with anxiety-like behavior. CBD either reversed or normalized these cue-induced molecular phenotypes. CBD also recruited lipid networks which correlated with transcripts involved in synaptic plasticity, signaling, and epigenetic mechanisms. These results suggest that CBD may specifically alleviate salient, conditioned anxiety and normalize related biological mechanisms in the NAcSh which may guide therapeutic interventions for anxiety disorders.

Are Women More Risk Averse? A Sequel

This paper reexamines the question of gender differences in financial relative risk aversion using updated methods and data. Specifically, the paper revisits the 1998 work “Are women more risk averse?” by Jianakoplos and Bernasek, suggests refinements in their model in relation to the database used, namely the U.S. Federal Reserve Board’s Survey of Consumer Finances (SCF), and performs new tests on the latest SCF from 2022. The suggested refinements pertain first to an enhanced computation of wealth, which includes additional categories of assets such as 401(k)s or other thrift savings accounts, and second to the more subtle handling and consideration of specific demographic data of the SCF respondents. Unlike the original study, which also included married couples, the new study focuses exclusively on single-headed (never-married) households. This eliminates ambiguity about the actual financial decision maker in households, enabling a clearer assessment of individual gendered behavior. Following the refinements, the new tests reveal a continuing pattern of decreasing relative risk aversion; however, contrary to the 1998 findings, there is no significant gender difference in financial relative risk aversion in 2022. This study also documents that education levels strongly influence risk-taking: single women with higher education levels are more likely to hold risky assets, while for men, higher education correlates with less risk-taking. The paper concludes by informing policymakers and financial educators so as to further tailor their strategies for promoting gender equality in financial decision-making.

Mechanical properties and microbial assessment of additively manufactured diamond and gyroid Ti-6Al-4V ELI scaffolds for interbody fusion cages applications

Abstract This study investigates the mechanical behaviour and microbial assessment of additively manufactured porous titanium scaffolds for interbody fusion cages applications. This study is meticulously designed to focus on both the mechanical and microbial assessments. Two types of cubic porous structure scaffolds were manufactured. The type 1 scaffold was designed with a diamond structure, featuring a pore size of 500 microns and 65% porosity. Similarly, the type 2 scaffold was designed with a gyroid structure, having a pore size of 500 microns and 65% porosity. Both the types of scaffolds were manufactured using direct selective laser sintering technique. Both the group of scaffolds tested for quasi static compression testing, fatigue compression testing, compression torsional testing and corrosion testing for mechanical properties evaluation. For microbial evaluation, bio burden tests, total organic carbon and total hydrocarbon tests were conducted on the both type of scaffolds. Static compression testing results reported that there is no significant effect on the compression strength properties. Diamond scaffolds had higher yield force value than gyroid scaffolds. Fatigue compression and compression - torsional results reported the higher endurance strength of diamond scaffolds over the gyroid scaffolds. There was no significant difference reported for corrosion behavior of both scaffolds. In the present study, both types of scaffolds demonstrated an almost negligible biological burden. The total organic carbon and hydrocarbons values reported were also under permissible limit as per ISO 19227.Results demonstrated several advantages such as higher endurance strength of diamond scaffolds over gyroid scaffolds, highly corrosion resistance and negligible bio burden limits.&amp;#xD;

Systematic Review and Meta‐Analyses Reveal no Gender Difference in Neonatal Social Perception

ABSTRACTWomen score higher than men on measures of social cognition such as empathy and reading non‐verbal cues. How early does this gender difference emerge? Systematic review and meta‐analyses were used to assess gender difference in social perception within 1 month of birth. A total of 31 studies (40 experiments) reported on gender effects in 1936 neonates (50% girls) between 1968 and 2021. No significant difference (Hedges’ g = 0.076, p = 0.321) was found across 20 experiments measuring visual fixation on human faces. Nine experiments on neonatal imitative crying also revealed no gender difference (g = 0.157, p = 0.118). Seven studies using the Neonatal Behavioral Assessment Scale found no gender difference in total orientation (g = 0.161, p = 0.154), but girls oriented more to both animate (g = 0.279, p = 0.011) and inanimate (g = 0.242, p = 0.003) stimuli in the studies that analyzed these separately. Existing evidence supports a possible maturational difference but not a specific social advantage for girls at birth. While more research and better reporting are needed, the present findings challenge the claim that girls are innately more socially perceptive than boys.

Behavioral and Electrophysiological Assessment of Central Auditory Processing in Individuals with Sickle Cell Disease.

Sickle cell anemia has a genetic origin characterized by an autosomal recessive inheritance pattern. The nervous system may be subject to vaso-occlusion and, consequently, affect the proper functioning of the central portion of hearing. To assess central auditory skills and analyze short- and long-latency auditory evoked potentials in children with sickle cell disease. Cross-sectional study. All children had normal hearing thresholds, and their central auditory processing underwent behavioral assessment with a battery of tests involving dichotic and monotic listening, binaural interaction, and temporal processing. The electrophysiological assessment used short- and long-latency auditory evoked potentials. Descriptive statistics were performed. Of the 28 subjects evaluated (mean age of 9.46years), 18 were females and 10 were males. Central auditory processing disorder was identified in 85.7% of the children. The auditory skills of figure-ground for verbal sounds, binaural interaction, and complex temporal ordering were the most affected. An increase in the absolute latencies of Waves III and V was observed in the short-latency potential. Individuals with sickle cell disease have central auditory processing disorder, identified primarily by behavioral assessment.

White Matter Fiber Bundle Alterations Correlate with Gait and Cognitive Impairments in Parkinson's Disease based on HARDI Data.

The neuroanatomical basis of white matter fiber tracts in gait impairments in individuals suffering from Parkinson's Disease (PD) is unclear. Twenty-four individuals living with PD and 29 Healthy Controls (HCs) were included. For each participant, two-shell High Angular Resolution Diffusion Imaging (HARDI) and high-resolution 3D structural images were acquired using the 3T MRI. Diffusion-weighted data preprocessing was performed using the orientation distribution function to trace the main fiber tracts in PD individuals. Clinical characteristics between the two groups were compared, and the correlation between the FA value and behavioral data was analyzed. Quantitative gait and clinical parameters were recorded in PD at ON and OFF states, respectively. The mean tract-specific FA values of the right Cingulum Cingulate (rCC) were statistically different between the PD group and the HC group (p =0.047). The FA value of 34-58 equidistant nodes in rCC was positively correlated with Mini-Mental State Examination (MMSE) (r=0.527, p=0.024), Berg Balance Scale (BBS)-OFF (r=0.480, p =0.040), and BBS-ON (r=0.528, p =0.024) scores, while it was negatively correlated with the MDS-UPDRS-III-ON score (r=-0.502, p =0.030). Regarding the gait analysis, the FA value was significantly correlated with velocity, cadence, and stride time of the pace and rhythm domains in both 'ON' and 'OFF' states, respectively (p<0.05). This study served as an initial exploration to establish that HARDI sequences could be employed as a robust tool for analyzing microstructural alterations in white matter fiber bundles among PD patients, although the sample size was small. We confirmed microstructural integrity impairment of rCC to be significantly associated with both gait and cognitive deficits in patients with PD. Early detection of microstructural changes in rCC and targeted treatment can help improve behavioral disorders. In the future, we intend to further integrate multimodal data with assessments of patient behavior both prior to and following intervention. We will validate our findings within an independent cohort to monitor disease progression and evaluate the efficacy of therapeutic interventions.

Cats with inflammatory gastrointestinal or dermatological disorders exhibit increased care-soliciting and anxious behaviors.

This study investigated whether the behavior profiles of cats with inflammatory gastrointestinal or skin diseases differ from those of healthy cats. We identified adult cats within the patient database at the University of Pennsylvania's veterinary hospital that had been diagnosed with inflammatory gastrointestinal (n = 22) or skin disorders (17) and a control group of healthy cats (58). We collected data via owner completion of the Feline Behavioral Assessment & Research Questionnaire from March to May 2023 and conducted an observational study comparing the Feline Behavioral Assessment & Research Questionnaire scores between groups. Cats with inflammatory gastrointestinal or skin disease exhibited more etepimeletic (care-soliciting) behaviors than healthy cats, including purring (U = 1,396.50, Z = 2.03, r = 0.21, 95% CI, 0.00 to 0.50), trainability (U = 1,303.50, Z = 2.33, r = 0.24, 95% CI, 0.00 to 1.00), and sociability to people (U = 367.50, Z = 2.21, r = 0.26, 95% CI, 0.00 to 1.57). The inflammatory group also exhibited more anxious behaviors than the healthy group, including compulsive grooming (U = 1,736.00, Z = 4.91, r = 0.50, 95% CI, 0.33 to 1.00) and fear of novelty (U = 603.00, Z = 2.14, r = 0.25, 95% CI, 0.00 to 1.00). Additionally, cats treated with corticosteroids exhibited more anxious behaviors than healthy and nonsteroid treatment groups, including separation behaviors (χ2[2] = 8.22, η2 = 0.08) and compulsive grooming (χ2[2] = 25.35, η2 = 0.25). Chronic inflammatory response is associated with increased etepimeletic and anxious behaviors in cats, and corticosteroid treatment is associated with anxious behaviors. These findings suggest that monitoring anxious and etepimeletic behaviors may be relevant when assessing inflammatory disease in cats.

Long-Term Exposure to Tire-Derived 6-PPD Quinone Causes Neurotoxicity and Neuroinflammation via Inhibition of HTR2A in C57BL/6 Mice.

N-(1,3-dimethylbutyl)-N'-phenyl-p-phenylenediamine quinone (6-PPDQ), a novel contaminant derived from tire wear, has raised concerns due to its potential neurotoxicity, yet its long-term effects on mammalian neurological health remain poorly understood. This study investigates the neurotoxic and neuroinflammatory impacts of prolonged 6-PPDQ exposure using male C57BL/6 mice. Behavioral assessments revealed significant cognitive deficits, while biochemical analyses demonstrated increased levels of reactive oxygen species, apoptosis, and blood-brain barrier (BBB) disruption. Elevated pro-inflammatory cytokines (TNF-α, IL-6, IL-1β) and activation of microglial cells were observed, indicating a robust neuroinflammatory response. Network pharmacology and molecular docking identified serotonin receptor HTR2A as a key target through which 6-PPDQ mediates its toxic effects. Activation of HTR2A by the agonist DOI (2,5-dimethoxy-4-iodoamphetamine) mitigated these effects, suggesting a potential therapeutic strategy. These findings provide the first evidence of 6-PPDQ-induced neurotoxicity in mammals, underscoring the need for preventive measures to protect neurological health.

Nutritional assessment of adolescents: A cross-sectional study from public schools of North India.

Technological advancements and globalization have shifted dietary behaviours, contributing to increased chronic disease prevalence in Low- and Middle-Income Countries (LMICs) like India. Adolescents are particularly vulnerable due to these changes, which can impact their lifelong health. This study aimed to assess the nutritional status of adolescents in public schools in Chandigarh, India. Conducted as part of a cluster randomized control trial, the study used two-stage random sampling to select 12 schools and eighth-grade classes, recruiting 453 adolescents aged 10-16 years. Nutritional status was evaluated through dietary behaviour assessments, anthropometric measurements, and 24-hour urinary salt-level analysis. Dietary patterns were recorded using two 24-hour recalls, and analyzed with PURE study software based on 2010 Indian dietary data from ICMR-NIN. Anthropometric measures followed standardized protocols, and salt levels were assessed in laboratories. The mean age of the adolescents was 13.06 years, with 55% being boys. Among them, 32% had high salt intake, and 55% had high sugar intake. Additionally, 90% had low fruit intake, and 83% had low vegetable intake. The adolescents were deficient in several macro and micronutrients, including energy, fats, fibre, iron, zinc, iodine, riboflavin, and vitamins B-6 and B-12. A higher proportion of boys (10%) were classified as thin compared to girls (2%), while a greater proportion of girls (36%) had abdominal obesity. In contrast, a larger proportion of boys (23%) were severely acutely malnourished. Nearly all adolescents exhibited high urinary excretory salt levels. Most adolescents exhibited dietary risk factors, including high salt and sugar intake, along with low consumption of fruits and vegetables. Many were deficient in various macro and micronutrients, with the coexistence of both thinness and obesity. Regular nutritional assessments in schools are essential to address the dual burden of undernutrition and overnutrition. Furthermore, health-promoting interventions should be developed within school settings to encourage healthy dietary practices.

3T dilated inception network for enhanced autism spectrum disorder diagnosis using resting-state fMRI data.

Autism spectrum disorder (ASD) is one of the complicated neurodevelopmental disorders that impacts the daily functioning and social interactions of individuals. It includes diverse symptoms and severity levels, making it challenging to diagnose and treat efficiently. Various deep learning (DL) based methods have been developed for diagnosing ASD, which rely heavily on behavioral assessment. However, existing techniques have suffered from poor diagnostic outcomes, higher computational complexity, and overfitting issues. To address these challenges, this research work introduces an innovative framework called 3T Dilated Inception Network (3T-DINet) for effective ASD diagnosis using resting-state functional Magnetic Resonance Imaging (rs-fMRI) images. The proposed 3T-DINet technique designs a 3T dilated inception module that incorporates dilated convolutions along with the inception module, allowing it to extract multi-scale features from brain connectivity patterns. The 3T dilated inception module uses three distinct dilation rates (low, medium, and high) in parallel to determine local, mid-level, and global features from the brain. In addition, the proposed approach implements Residual networks (ResNet) to avoid the vanishing gradient problem and enhance the feature extraction ability. The model is further optimized using a Crossover-based Black Widow Optimization (CBWO) algorithm that fine-tunes the hyperparameters thereby enhancing the overall performance of the model. Further, the performance of the 3T-DINet model is evaluated using the five ASD datasets with distinct evaluation parameters. The proposed 3T-DINet technique achieved superior diagnosis results compared to recent previous works. From this simulation validation, it's clear that the 3T-DINet provides an excellent contribution to early ASD diagnosis and enhances patient treatment outcomes.

Fecal Microbiota Transplantation (FMT) From a Human at Low Risk for Alzheimer's Disease Improves Short-Term Recognition Memory and Increases Neuroinflammation in a 3xTg AD Mouse Model.

Human microbiota-associated murine models, using fecal microbiota transplantation (FMT) from human donors, help explore the microbiome's role in diseases like Alzheimer's disease (AD). This study examines how gut bacteria from donors with protective factors against AD influence behavior and brain pathology in an AD mouse model. Female 3xTgAD mice received weekly FMT for 2 months from (i) an 80-year-old AD patient (AD-FMT), (ii) a cognitively healthy 73-year-old with the protective APOEe2 allele (APOEe2-FMT), (iii) a 22-year-old healthy donor (Young-FMT), and (iv) untreated mice (Mice-FMT). Behavioral assessments included novel object recognition (NOR), Y-maze, open-field, and elevated plus maze tests; brain pathology (amyloid and tau), neuroinflammation (in situ autoradiography of the 18 kDa translocator protein in the hippocampus); and gut microbiota were analyzed. APOEe2-FMT improved short-term memory in the NOR test compared to AD-FMT, without significant changes in other behavioral tests. This was associated with increased neuroinflammation in the hippocampus, but no effect was detected on brain amyloidosis and tauopathy. Specific genera, such as Parabacteroides and Prevotellaceae_UGC001, were enriched in the APOEe2-FMT group and associated with neuroinflammation, while genera like Desulfovibrio were reduced and linked to decreased neuroinflammation. Gut microbiota from a donor with a protective factor against AD improved short-term memory and induced neuroinflammation in regions strategic to AD. The association of several genera with neuroinflammation in the APOEe2-FMT group suggests a collegial effect of the transplanted microbiome rather than a single-microbe driver effect. These data support an association between gut bacteria, glial cell activation, and cognitive function in AD.

Kuanxiong Aerosol Attenuates Ischemic Stroke Injury via Modulation of the TRPV1 Channel.

To evaluate the therapeutic effects of Kuanxiong Aerosol (KXA) on ischemic stroke with reperfusion and elucidate the underlying pharmacological mechanisms. In vivo pharmacological effects on ischemic stroke with reperfusion was evaluated using the transient middle cerebral artery occlusion (t-MCAO) mice model. To evaluate short-term outcome, 30 mice were randomly divided into vehicle group (n=15) and KXA group (n=15). Mice in KXA and vehicle groups received 69 mg KXA and vehicle for 1 day, respectively. To evaluate long-term outcome, 35 mice were randomly divided into sham group (n=5), vehicle group (n=15), and KXA group (n=15). Mice in KXA and vehicle groups received 69 mg KXA and vehicle for 7 days, respectively. Pathological changes in the brain were observed by 2,3,5-triphenyltetrazolium chloride or Nissl stainings, and behavioral assessments, including the Modified Neurologic Severity Score, Bederson score, rotarod test, and adhesive removal test were conducted. The penetration ability of KXA and KX (KXA without propellants) through the blood-brain barrier was assessed both in vitro using a transwell model and in vivo. Furthermore, in vitro effects of KX (5, 10, and 20 µL/L) on oxygen and glucose deprivation/re-oxygenation (OGD/R)-induced injury, transient receptor potential vanilloid type 1 (TRPV1) modulation, calcium influx, and mitochondrial function were explored through Western blot, CCK-8 assay, JC-1 staining, calcium imaging, adenosine triphosphate (ATP) and antioxidant measurements. In in vivo experiments, KXA reduced brain infarct volume and neuron loss in t-MCAO mice. Behavioral assessments showed marked improvement in the neurological deficit of t-MCAO mice with KXA treatment (P<0.05 or P<0.01). Additionally, in vitro findings indicated that KX ameliorated OGD/R-induced injury through TRPV1 channel modulation. KX increased cell viability in OGD/R-treated SH-SY5Y cells and prevented OGD/R-induced calcium overload by downregulating TRPV1 expression and constraining calcium influx through TRPV1 (P<0.05 or P<0.01). Furthermore, KXA maintained the membrane potential and function of mitochondria in OGD/R-treated SH-SY5Y cells. KXA could attenuate ischemic stroke injury through TRPV1 channel modulation, indicating its potential as a promising therapeutic option for stroke in clinical practice.

The role of interoceptive sensibility on central sensitization to pain in vulvodynia.

Interoception may be linked to central sensitization in chronic pain. We aimed to provide evidence about the role of interoceptive sensibility on central sensitization in vulvodynia. In this cross-sectional study, a sample of females who received a diagnosis of vulvodynia filled out validated questionnaires relative to the individual level of interoceptive sensibility and the symptoms of central sensitization. Interoceptive sensibility and symptoms of central sensitization were measured with the Multidimensional Assessment of Interoceptive Awareness and the Central Sensitization Inventory, respectively. A lower level of trust and a higher level of emotional awareness predicted a higher number of central sensitization symptoms in our sample. Our evidence may increase the researchers' and physicians' attention toward the involvement of the central nervous system in pain phenomenology in vulvodynia. No ad-hoc control sample was collected. No behavioral assessments about interoception were performed. As registered in other chronic pain conditions, interoceptive sensibility may play a crucial role in the expressions of symptoms of central sensitization in vulvodynia.