Assessment Scheme Research Articles

Technical and health governance aspects of the External Quality Assessment Scheme for the SARS-CoV-2 molecular tests: institutional experience performed in all clinical laboratories of a Regional Health Service.

Since December 2019, the worldwide public health has been threatened by a severe acute respiratory syndrome caused by Coronavirus-2. From the beginning, a turning point has been the identification of new cases of infection, in order to minimize the virus spreading among the population. For this reason, it was necessary introducing a panel of tests able to identify positive cases, which became crucial for all countries. As a Regional Reference Centre, the CRQ Laboratory (Regional Laboratory for the Quality Control) developed and conducted an External Quality Assessment (EQA) panel of assay, so as to evaluate the quality of real-time reverse transcription polymerase chain reaction (PCR), which were used by 62 Sicilian laboratories, previously authorized to issue certificates for the COVID-19 diagnosis, on behalf of the Public Health Service. The qualitative performance test was based on pooled samples with different viral loads of SARS-CoV-2 or human Coronavirus OC43. 75% of the participating laboratories tested all core samples correctly, while the remaining 25% interpreted incorrectly the EQA exercise samples matching negatively the standards required. Subsequent inspection visits confirmed the issue of incorrect positive and negative certifications for COVID-19 by private and public laboratories, despite the possession of the authorization requirements currently provided for by current regulations, with a significant impact on the SSR.

Towards ‘one substance-one assessment’: an analysis of European chemical registration and environmental risk assessment frameworks

BACKGROUND AND AIM The registration and risk assessment of chemicals on the European market is currently fragmented across different legal frameworks, dependent on the chemical's use. A ‘one-substance one-assessment’ (OS-OA) approach has been proposed in order to better protect human and environmental health, and increase harmonisation and consistency across these different legal frameworks. We reviewed the five main European chemical registration frameworks and their risk assessment procedures for the freshwater environment, covering 1) medicines for human use, 2) veterinary medicines, 3) pesticides, 4) biocides and 5) industrial chemicals in order to provide an understanding of the differences between these chemical legislations and analyse how a shift towards an OS-OA approach might be realised. RESULTS Overall, the function of the current frameworks is similar, but important differences exist between the frameworks' environmental protection goals and risk assessment strategies. These differences result in inconsistent assessment outcomes for similar chemicals. Chemicals are also registered under multiple frameworks due to their multiple uses, and chemicals that are not approved under one framework are in some instances allowed on the market under other frameworks. In contrast, an OS-OA will require a uniform hazard assessment between all different frameworks. In addition, we show that industrial chemicals are the least hazardous for the freshwater environment, whilst biocides seem the most toxic following current regulatory assessment schemes. Finally, in order to facilitate a successful move towards an OS-OA approach we recommend a) harmonisation of protection goals and risk assessment strategies, b) that emission, use and production data should be made publicly available and that data sharing becomes a priority, and c) an alignment of the criteria used to classify problematic substances. KEYWORDS EU chemical legislation, Regulatory risk assessment, Water pollution, Environmental risk assessment

Formative-reflective scheme for the assessment of tourism destination competitiveness: an analysis of Italian municipalities

In this article, we propose a formative-reflective scheme for the assessment of Tourism Destination Competitiveness (TDC) based on a combined use of Partial Least Squares-Path Modelling (PLS-PM) and the method recently proposed by Fattore, Pelagatti, and Vittadini (FPV). TDC is conceived as a construct reflecting the tourism performance of a destination, and several determinants are considered, including endowed resources, created resources, and supporting factors. The proposed scheme is applied to a case study on 1575 Italian municipalities for which the Italian National Institute of Statistics released data on tourist flows. Our contribution is innovative for three aspects: (i) the consistency of the formative-reflective scheme for TDC assessment is discussed on a theoretical basis; (ii) an empirical comparison between PLS-PM and the FPV method is performed; (iii) data with higher granularity than most studies on TDC assessment are employed. Our findings highlight that endowed resources are the primary driver of TDC, followed by created resources and supporting factors, and emphasize that the best ranked destinations are big cities with a multifaceted tourism alongside sea and mountain destinations with cultural attractions.

UK NEQAS Haematology pilot scheme: Reticulocyte haemoglobin content quantitation.

Established parameters for the investigation of suspected iron deficiency have recognized limitations that affect their sensitivity and specificity. Reticulocyte haemoglobin content (RHC) is an early biomarker of iron deficiency or restriction, which also demonstrates response to iron therapy. RHC parameters are offered on all major automated haematology analysers. There is no external quality assessment (EQA) for RHC in the UK, restricting its wider clinical application. The UK National External Quality Assessment Scheme (UKNEQAS) for Haematology has completed a pilot study on the use of stabilized blood for RHC EQA. Blood was obtained from two JAK2 V617F mutation-positive, polycythaemia vera patients undergoing regular venesection, and from a healthy volunteer donor. Aliquots of each donation were distributed to users of Abbott, Horiba, Siemens and Sysmex automated analysers for RHC analysis on days 1, 3, 5 and 7. Results were received from 20 laboratories using four different platforms. The daily mean and standard deviation (SD) were calculated for each aliquot, by day of analysis and platform. With RHC there was no significant within-platform difference (p> 0.5) between testing days, although there were statistically significant differences (p< 0.001) between different platforms. The greatest difference was seen between Abbott MCHr and Horiba RhCc (-6.14 ± 1.25 pg). Despite inter-platform differences, it was possible to define iron deficient, borderline, and normal cut-offs to classify 95% of results correctly. The results demonstrate that it is possible to provide RHC EQA material using UKNEQAS standard procedures. The results are clinically relevant but indicate a requirement for inter-user comparison.

Food analysis: a practical guide

Food analysis: a practical guide

Sub-lethal doses of sulfoxaflor impair honey bee homing ability.

Agricultural intensification has increased the number of stressors that pollinators are exposed to. Besides increasing landscape fragmentation that limit the supply of flower resources, intensive agricultural practices relying on the use of pesticides to control agricultural pests also affect non-target organisms like honey bees. The use of most pesticides containing neonicotinoids has been severely restricted in the European Union, leaving pesticides containing acetamiprid as the only ones that are still authorized. In the meantime, new substances like sulfoxaflor, that have a similar mode of action acting on the insect's nicotinic acetylcholine receptors (nAChR), have been approved for agricultural use. In Europe and USA, the use of pesticides containing this active ingredient is limited due to toxic effects already reported on bees, but no restrictions regarding this matter were applied in other countries (e.g., Brazil). In this study, homing ability tests with acetamiprid and sulfoxaflor were performed, in which honey bees were fed with three sub-lethal doses from each substance. After exposure, each honey bee was equipped with an RFID chip and released 1 km away from the colony to evaluate their homing ability. No significant effects were detected in honey bees fed with 32, 48 and 61 ng of acetamiprid while a poor performance on their homing ability, with only 28% of them reaching the colony instead of 75%, was detected at a 26 ng/a.s./bee dose of sulfoxaflor. Although, both pesticides act on the nAChR, the higher sulfoxaflor toxicity might be related with the honey bees detoxifying mechanisms, which are more effective on cyano-based neonicotinoids (i.e., acetamiprid) than sulfoximines. With this study we encourage the use of homing ability tests to be a suitable candidate to integrate the future risk assessment scheme, providing valuable data to models predicting effects on colony health that emerge from the individual actions of each bee.

External Quality Assessment Data for Investigation of von Willebrand Disease: Focus on Relative Utility of Contemporary Functional von Willebrand Factor Assays. The United Kingdom National External Quality Assessment Scheme (UK NEQAS) Experience.

Von Willebrand disease (VWD) is one of the most common hereditary bleeding disorders. Effective management of patients and their families depends on accurate diagnosis and subtype classification, and quality assurance including participation in proficiency testing programs is essential to ensure the accuracy of the panel of assays required to achieve this diagnosis. We report here findings from recent external quality assessment (EQA) exercises, as well as from a questionnaire about diagnostic practices employed by centers in the United Kingdom National Quality Assessment Scheme (UK NEQAS) performing von Willebrand factor (VWF) assays. Plasma samples from donors with VWD, "normal" donors, the International Society for Thrombosis and Haemostasis Scientific Subcommittee (ISTH SSC) plasma standard, and whole blood samples were sent to participants in the UK NEQAS BC program for VWF investigation. Calibration of lot#5 of the ISTH SSC plasma standard was shown to give improved comparability between the recovered value from an EQA exercise and the assigned potency for VWF activity assays. Diagnostic accuracy and precision amongst UK NEQAS participants was good, with an average 99% of centers reporting the correct interpretation for normal, type 1 and type 2 VWD samples, 100% diagnostic accuracy for centers performing FVIII binding assays, and good agreement amongst centers performing multimeric analysis. Genetic analysis of the VWF gene by specialist centers demonstrated errors in the genotyping process in one center, but also demonstrated failings in the interpretation of results in other centers. Despite evidence of good laboratory accuracy and precision in their assays, a questionnaire identified marked variation in diagnostic criteria employed, underlining the importance of guidelines to support the diagnosis of VWD.

Development and validation of diagnostic algorithms for the laboratory diagnosis of porphyrias.

Porphyrias are rare metabolic disorders of the haem synthesis. They can present with acute neurovisceral attacks, cutaneous symptoms, or a combination of both. As they present with a wide variety of clinical symptoms, diagnosis is often delayed and correct interpretation of porphyria-related tests remains a challenge for many physicians. We developed and validated two algorithms for the laboratory diagnosis of porphyrias based on presenting symptoms. Based on a literature search and clinical/laboratory expertise, we developed algorithms for acute and cutaneous porphyrias. We validated these algorithms using all porphyria related laboratory test requests between January 1st 2000 and September 30th 2020 in UZ Leuven. In addition, we also evaluated our algorithm using samples from the European porphyria network (EPNET) external quality assessment scheme (2010-2021). Sensitivity of the algorithm for acute porphyria was 100.0% [74.9%-100.0%] (13 acute intermittent porphyria (AIP) and 1 variegate porphyria [VP]) with a specificity of 98.5% [91.0%-100.0%] (65 patients). Sensitivity of the algorithm for cutaneous porphyria was 100% [95.1%-100.0%] (7 VP, 59 porphyria cutanea tarda (PCT), 23 erythropoietic protoporphyria (EPP), 2 X-linked erythropoietic protoporphyria [XLEPP]) with a specificity of 93.9% [82.9%-98.5%]. There were no diagnostic samples of other types of porphyria. The algorithms correctly identified 18 of the 19 EPNET porphyria cases. One of the two hereditary coproporphyria cases was missed. The algorithms for acute and cutaneous porphyria showed high sensitivity and specificity and can be used to aid the clinician in correctly interpreting the laboratory findings of porphyria-related tests.

Predicted Dermal and Dietary Exposure of Bats to Pesticides.

Wild birds and mammals that feed in agricultural habitats are potentially exposed to pesticides through various routes. The European Food Safety Authority (EFSA) recently published a statement which concluded that the current EFSA risk assessment scheme for birds and mammals does not adequately cover bats (Chiroptera). In the present study, we take a more detailed look at the EFSA statement and assumptions made regarding direct (dermal) and indirect (dietary) exposure of bats to pesticides in terms of their realism and the potential implications for risk assessment outcomes. Regarding dietary exposure, errors in the residue per unit dose (RUD) values for flying insects (bat food), proposed in the EFSA bat statement, were identified and corrected. Lower RUD values based on a much broader data base are proposed. Using these more realistic RUD values, together with current assumptions regarding toxicity and exposure, the acute and long-term risk to bats appears to be within the range of those calculated for birds and ground-dwelling mammals under the current risk assessment scheme. Depending on the assumptions made, some uncertainties may remain and should be investigated further. According to the EFSA bat statement, dermal exposure of bats is the most significant route of exposure, resulting in the highest predicted daily doses. We demonstrated that the dermal exposure models in the EFSA bat statement predict much higher residues for bats than those measured for other flying organisms that have larger surface area to volume ratios, and thus would be expected to have the reverse relationship. We also illustrated that the amounts of spray liquid required to achieve the predicted dermal exposures of bats are implausibly high, with bats carrying an amount of spray liquid that exceeds their body weight many fold. It is recommended that a bat risk assessment framework should be based on realistic, sound science, allowing resources to be focused on those scenarios that are not already covered by the existing bird and mammal framework. Therefore, a quantitative risk assessment scheme should not be implemented until the many scientific uncertainties within the EFSA bat statement are addressed. Environ Toxicol Chem 2022;41:2595-2602. © 2022 Cambridge Environmental Assessments. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.

Measurement of serum uric acid by isotope dilution liquid chromatography tandem mass spectrometry: Modification of a candidate reference measurement method and its clinical application

Uric acid is a precipitating factor for gout and renal disease, and regular monitoring of uric acid concentration is essential for health management. Hence, accurate measurement is indispensable for clinical diagnosis. Reference measurement procedure (RMP) is an essential element to ensure accuracy and comparability of tested results. In this study, the optimization of a candidate RMP (cRMP) adopting isotope dilution coupled with liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS) method for human serum uric acid measurement has been developed. The accuracy of this cRMP was validated by measuring certified reference materials (CRMs) and participating in the external quality assessment (EQA) scheme initiated by International Federation of Clinical Chemistry (IFCC) and National Center for Clinical Laboratory (NCCL). Biases were −0.64-1.51%, which were within the required equivalent limit. Excellent precision was obtained with intra- and inter-assay CVs of 0.42–0.73% and 0.20–0.43%, respectively. The limit of detection (LOD) and limit of quantification (LOQ) was determined to be 0.24 and 1.19 μmol/L, respectively. A good liner response was obtained ranged from 30.0 to 895 μmol/L. Compared with the previous RMP for uric acid, the much easier one-step protein precipitation method without further evaporation, the simplified isocratic elution of chromatographic separation condition, and the shorter analysis running time in only 5 min allowed simpler and faster analysis. Moreover, this optimized cRMP was also applied for value assignment for clinical serum samples covered a wide concentration range of uric acid and obtained a decent calibration and measurement capability (CMC).

Confronting the unknown-Nursing surveillance of COVID-19-infected patients through remote telephone calls and in an on-site urgent clinic.

AimTo describe nursing surveillance of coronavirus disease 2019 (COVID‐19)‐infected patients through remote telephone calls and in an on‐site urgent clinic during the first wave of the pandemic as experienced by nurses providing the care.DesignQualitative descriptive study.MethodsData were collected through seven semi‐structured, audio‐recorded, focus group interviews with 24 nurses. Interviews were conducted in May and June 2020, transcribed and analysed using deductive and inductive content analysis into an overarching category, main categories and subcategories. Reporting followed the COREQ guidelines.ResultsNurses relied on intensive listening when assessing and caring for COVID‐19‐infected patients. They realized that the patients had complex needs for nursing and healthcare which was beyond the scope of a tentatively prescribed assessment scheme. They designed their care to ensure holistic care, reflected in the overarching category, ‘Confronting an unfamiliar health condition in unprecedented circumstances’ and the categories: ‘Digging into the unknown’ and ‘Ensuring holistic nursing care’. The category ‘Contributing to averting catastrophe’ reflects the wealth of knowledge, support and experience that the nurses used to independently deliver care, albeit in interdisciplinary collaboration, working to their greatest potential. They were proud of the significance of their work.ConclusionNovel nursing surveillance through remote telephone calls and in an on‐site urgent care clinic delivered to COVID‐19 patients self‐managing at home resulted in holistic nursing care during the first wave of the pandemic. This has relevance for professionalism in nursing.ImpactFindings give a unique insight into nursing surveillance of COVID‐19‐infected patients provided through telephone calls and in on‐site urgent care clinics. The potential of intensive listening as conducted in the study suggests that it may be feasible to assess and holistically take care of COVID‐19‐infected patients, and other patient groups as well, with this form of healthcare. This has relevance for healthcare beyond crisis management during pandemics.Patient or Public ContributionThere was no patient or public contribution as the study only concerned the providers of the service, i.e. the nurses themselves.

Towards unified management of software capstone projects in Saudi universities: a survey-based study

PurposeSaudi universities have incorporated capstone projects in the final year of an undergraduate study. Although universities are following recommendations of the National Commission for National Commission for Academic Accreditation and Assessment (NCAAA) and Accreditation Board for Engineering and Technology (ABET), no detailed guidelines for management and assessment of capstone projects are provided by these accreditation bodies. Variation in the management and assessment practices of capstone project courses and analysis of the students' capabilities to align with industry demands, to realize Vision 2030, is challenging. This study investigates the current practices for structure definition, management and assessment criteria used for capstone project courses at undergraduate level for information technology (IT) programs at Saudi universities.Design/methodology/approachA web-based questionnaire is administered using a web service commonly used for questionnaires and polls to investigate the structure, management and assessment of capstone projects at the undergraduate level offering software engineering, computer science and information technology (SECSIT) programs. In total, 42 faculty members (with range of experience of managing/advising capstone projects from 1 to more than 10 years) from 22 Saudi universities (out of more than 30 universities offering SECSIT undergraduate programs) participated in the study.FindingsThe authors have identified that Saudi universities are facing challenges in the utilized process model, the distribution of work and marks, the knowledge sharing approach and the assessment scheme. To cope with these challenges, the authors recommend the use of an incremental development process, the utilization of a project-driven approach, the development of a national level digital archive and the implementation of homogeneous assessment scheme.Social implicationsTo contribute to the national growth and to fulfill the market demand, universities are recommended to align the capstone project courses with latest technology trends. Universities must collaborate with the industry and update the structure and requirements of capstone project courses accordingly. This will further facilitate to bridge the gap between industry and academia and will develop a win–win scenario for all the stakeholders.Originality/valueAlthough universities are committed to increase innovative capacities of their students for enabling them to contribute to economic and social growth, it is still hard to know the knowledge creation and sharing at national level. Variations in the management and assessment practices for capstone projects further intensify this challenge. Hence, there is a need of smart assessment and management of software capstone projects being developed in Saudi universities. Incorporating latest technologies, such unified management can facilitate discovering the trends and patterns related to the domain and complexity.

Data-driven flexibility assessment for internet data center towards periodic batch workloads

Considering its unique operational and power consumption characteristics, internet data center (IDC) has been intensively investigated as a promising candidate to provide flexibility for electric power system. In this paper, a data-driven flexibility assessment scheme for IDC is proposed by investigating the temporal shifting capability of periodic batch workloads, which are the major flexibility source in the workload scheduling and execution process. We develop a four-step assessment procedure by identifying the periodic jobs, extracting key operational patterns, mapping the power consumption with workload execution, and quantifying the flexibility associated with power system operation, all of which are established in a data-driven manner. In addition, we adopt real-world production workload trace to verify and demonstrate the effectiveness of the proposed flexibility assessment scheme.

Effects of chronic exposure to the new insecticide sulfoxaflor in combination with a SDHI fungicide in a solitary bee

The recent EU ban of the three most widely used neonicotinoids (imidacloprid, thiamethoxam and clothianidin) to all outdoors applications has stimulated the introduction of new insecticides into the market. Sulfoxaflor is a new systemic insecticide that, like neonicotinoids, acts as a modulator of nicotinic acetylcholine receptors. In agro-environments, bees can be exposed to this compound via contaminated pollen and nectar for long periods of time. Therefore, it is important to assess the potential effects of chronic exposure to sulfoxaflor, alone and in combination with fungicides, on pollinators. In this study, we tested the effects of chronic exposure to two field concentrations of sulfoxaflor (20 and 100 ppb) alone and in combination with four concentrations of the fungicide fluxapyroxad (7500, 15,000, 30,000 and 60,000 ppb) on syrup consumption and longevity in females of the solitary bee Osmia bicornis L. Exposure to 20 ppb of sulfoxaflor, alone and in combination with the fungicide, stimulated syrup consumption, but did not affect longevity. In contrast, syrup consumption decreased in bees exposed to 100 ppb, all of which died after 2–6 days of exposure. We found no evidence of synergism between the two compounds at any of the two sulfoxaflor concentrations tested. Comparison of our findings with the literature, confirms that O. bicornis is more sensitive to sulfoxaflor than honey bees. Our results highlight the need to include different bee species in risk assessment schemes.

The Significance of External Quality Assessment Schemes for Molecular Testing in Clinical Laboratories.

Simple SummaryPatients and clinicians often rely on the outcome of laboratory tests, but can we really trust these test results? Good quality management is key for laboratories to guarantee reliable test results. This review focusses on external quality assessment (EQA) schemes which are a tool for laboratories to examine and improve the quality of their testing routines. In this review, an overview of the role and importance of EQA schemes for clinical laboratories is given, and different types of EQA schemes and EQA providers available on the market are discussed, as well as recent developments in the EQA landscape.External quality assessment (EQA) schemes are a tool for clinical laboratories to evaluate and manage the quality of laboratory practice with the support of an independent party (i.e., an EQA provider). Depending on the context, there are different types of EQA schemes available, as well as various EQA providers, each with its own field of expertise. In this review, an overview of the general requirements for EQA schemes and EQA providers based on international guidelines is provided. The clinical and scientific value of these kinds of schemes for clinical laboratories, clinicians and patients are highlighted, in addition to the support EQA can provide to other types of laboratories, e.g., laboratories affiliated to biotech companies. Finally, recent developments and challenges in laboratory medicine and quality management, for example, the introduction of artificial intelligence in the laboratory and the shift to a more individual-approach instead of a laboratory-focused approach, are discussed. EQA schemes should represent current laboratory practice as much as possible, which poses the need for EQA providers to introduce latest laboratory innovations in their schemes and to apply up-to-date guidelines. By incorporating these state-of-the-art techniques, EQA aims to contribute to continuous learning.

Results of a European-Wide External Quality Assessment (EQA) Scheme for Serological Detection of Anti-SARS-CoV-2 (CoVimm)-Pitfalls of Routine Application.

Background: During the last two years, a variety of assays for the serological detection of antibodies to the new SARS-CoV-2 virus have been launched and used as part of standard care in many laboratories. The pace with which these tests have been introduced into routine care emphasizes the importance of quality measures for analytical methods, particularly with regard to the implications of results for clinical and epidemiologic decisions. Accuracy, reliability and comparability of analytical test results are thus essential, and here external quality assessment (EQA) is the most important quality assurance tool. It allows us to achieve harmonization of test methods as a prerequisite for a high standard of performance for laboratory and analytical techniques and their interpretation. Methods: This EQA scheme consisted of pre-characterized clinical biospecimens dedicated to the analysis of anti-SARS-CoV-2 IgG total antibodies and differentiation into spike protein-specific IgG antibodies against SARS-CoV-2 (anti-S-SARS-CoV-2) and nucleocapsid-specific IgG antibodies against SARS-CoV-2 (anti-N-SARS-CoV-2). Results: A total of 239 laboratories across Europe participated in this scheme, called CoVimm. In detail, 536 results for anti-SARS-CoV-2 IgG, 431 results for anti-S-SARS-CoV-2 IgG, and 200 results for anti-N-SARS-CoV-2 IgG were reported. Based on the pre-defined thresholds, the success rates for the determination of anti-S-SARS-CoV-2 IgG and anti-N-SARS-CoV-2 IgG were 96% and 90%, respectively. Interestingly, only 64% of the participating laboratories successfully passed the EQA scheme for the determination of total anti-SARS-CoV-2 IgG. Conclusions: This EQA revealed serious concerns regarding the reliability and appropriate use of anti-SARS-CoV-2 antibody assays in routine care. In addition to the wide heterogeneity of different assays used by participating laboratories, a lack of standardization and harmonization is also evident. This is of particular importance for reliable and clinically meaningful interpretation of test results.

Study on an Integrated LCA-LCC Model for Assessment of Highway Engineering Technical Schemes

This paper proposes an integrated life cycle assessment-life cycle cost (LCA-LCC) model of environmental and economic factors for highway engineering technical schemes to problems such as the limitations of single-dimensional assessment, their narrow scope, the difficulty in tracing sources, and the conflicts of various dimensions in existing integrated assessment methods. The latest documents issued by the Ministry of Ecology and Environment and the Ministry of Transport of China used as an integrated assessment database. Air pollution, water pollution, solid waste pollution, noise pollution, energy consumption, pre-project cost, project construction cost, project operation cost, and post-project cost were used to construct the integrated assessment index system of environmental and economic factors. An improved entropy method was adopted in the LCA-LCC model to overcome the problems of ambiguous results of the previous entropy due to too few assessment schemes, the inoperability of the method when it encounters a negative value or zero value, and unbalanced multi-angle assessments. This model was applied to the assessment of two asphalt pavement maintenance schemes of Highway US280 in Alabama and two improvement schemes of high liquid limit soil subgrade of Highway G360 in Hainan. The results show that the LCA-LCC model overcomes the limitations and imbalances of a single LCA or LCC. The gravel improved scheme and the cold recycling scheme were identified through quantitative assessment as more sustainable. This paper can provide a reference for the comprehensive quantitative assessment of environmental and economic benefits of highway engineering technical schemes.

An external quality assessment for the molecular testing of the SARS-CoV-2 virus genome in Zhejiang Province, China

The COVID-19 pandemic has necessitated the rapid expansion of laboratories that conduct SARS-CoV-2 tests. A provincial external quality assessment (EQA) scheme on SARS-CoV-2 tests was organized by Zhejiang Provincial CDC to assess the accuracy of the tests in individual CDC municipal and county laboratories in Zhejiang Province, China. Three positive samples in high, medium, and low concentrations, respectively, were prepared using the serial dilutions from the culture with the viral titer concentration of 1×106.3 TCID50/mL, and one negative sample were included. A total of 93 laboratories participated, contributing results from 36 distinct combinations of nucleic acid extraction methods and PCR reagents. There was 100% concordance among all laboratories for all EQA samples, and no false-positive or false-negative results were observed. The EQA survey provides confidence in the identification of infected individuals or asymptomatic populations and assurance for clinical and public health decision-making based on test results.

Novel continuous learning scheme for online static security assessment based on the weather‐dependent security index

Novel continuous learning scheme for online static security assessment based on the weather‐dependent security index

A decentralized operating performance assessment for geological drilling process via multi-block total projection to latent structures and Bayesian inference

Geological drilling process is an industrial process that contains a lot of variables, and the relationships and characters among them are also complex. Accordingly, implementing operating performance assessment by conventional methods has always been a problem that operators are dedicated to solving. In fact, geological drilling process is a complex industrial process involving multiple systems and stratigraphic uncertainties, which makes assessing its operating performance difficult. A decentralized operating performance assessment based on multi-block total projection to latent structures (T-PLS) and Bayesian inference is proposed for the geological drilling process. Utilizing the variational trends of the detection variables, the most related variables can be grouped in the same block, and the process capability index determines the operating performance grade. This is followed by a T-PLS algorithm-based operating performance assessment model and Bayesian inference. Each sub-block’s assessment results are integrated to achieve a comprehensive performance assessment. Last but not least, drill data show that the proposed method is effective and superior. The proposed method provides better accuracy and generalizability in assessing drilling performance. The novelty of the assessment scheme involves that a decentralized framework is proposed for operating performance assessment by identifying normal operating conditions first and then constructing the multi-block T-PLS-based monitoring model on the local sub-blocks.