Assessment Of Motor Activity Research Articles

Bedside electromyography for clinical assessment of sacral motor and reflex activity adapted for patients hospitalized with acute neurological conditions: a pilot study.

Pilot cohort study. To develop and implement a sacral electromyographic (sEMG) technique at bedside to ascertain sparing of sacral motor activity and reflexes in patients hospitalized for acute neurological conditions. Hôpital du Sacré-Coeur de Montréal a Canadian Level-1 university trauma center specialized in SCI care. Nine patients underwent digital rectal examination (DRE) and sEMG, assessing voluntary anal contraction and sacral spinal reflexes (bulbocavernosus reflex and the anal wink). Our sEMG technique utilized surface recording electrodes and tactile elicitation of reflexes. EMG signal was acquired at bedside through the Noraxon MR3 system. It was quick, well accepted and did no harm. We found that contrary to the DRE, sEMG detected subclinical sacral motor activity and reflexes in 20% of cases for voluntary anal contraction and 40% of cases for the anal wink. We believe our sEMG technique is a powerful tool able to enhance management of patients suffering from acute neurological impairments and requiring sacral function assessment.

Assessment of motor activity of patients undergoing medical rehabilitation

Assessment of motor activity of patients undergoing medical rehabilitation

Long-term motor activity, cardiopulmonary performance and quality of life in abdominal wall defect patients

AimTo assess whether patients born with an abdominal wall defect (AWD) have impaired cardiorespiratory performance capacity, motor skills, core stability or quality of life in a long-term follow up.MethodsPatients diagnosed with AWD between 2002 and 2013 were invited to participate in the study, which included clinical examination, spirometry, cardiopulmonary exercise performance testing, assessment of motor activity, ultrasound, electromyography of the abdominal wall and assessment of the Gastrointestinal Quality of Life Index (GIQLI). The results were compared to a healthy control group matched for age, sex, BMI, and physical activity levels.ResultsIn total, 18 AWD patients (mean age 12.6 ± 3.5 years) were included and there were no significant differences in anthopometric data compared to the control group (n = 18). AWD patients had a significantly lower GIQLI score (AWD mean 137.2 ± 6.8 vs. control mean 141.4 ± 4.9; p = 0.038) and were affected by decreased motor abilities with significantly higher Dordel-Koch-Test values (AWD median 3.54/IQR 1 vs. control median 2.8/IQR 1; p = 0.005).ConclusionFollow-up examinations of AWD patients revealed decreased motor abilities and GIQLI scores while cardiopulmonary function was not different compared to healthy controls. The clinical impact of these findings remains to be elucidated.ImpactClinical examination, assessment of the gastrointestinal quality of life, sport medical testing, electromyography and abdominal wall ultrasound were performed in patients with congenital abdominal wall defect and compared to an age and sex matched healthy control group.Results of spirometry and spiroergometry, ultrasound or electromyography did not significantly differ between the groups.Significantly decreased locomotor function and gastrointestinal quality of life were found in patients with abdominal wall defect. However, the clinical impact of these findings remains to be elucidated.

Physical Activity and Exercise in Patients with Epilepsy: a Single-Centre Uncontrolled Retrospective Cohort Study

INTRODUCTION. For a long time, persons with epilepsy have been told to stay away from physical activity and sports to prevent seizure triggers. However, in recent years, evidence has accumulated to the contrary, suggesting that sports may help with preventing seizures.
 AIM. To examine the level of physical activity and sports among patients of the Russian Epileptology Center.
 MATERIALS AND METHODS. We conducted a study on physical activity in patients with childhood epilSepsy and in patients currently suffering from epilepsy in 2018–2019 at Polenov Neurosurgical Institute. They answered 3 blocks of questions: first block — physical activity and sporting activity during the school years, second block — current assessment of motor activity, third block — effect of physical activity on the course of the disease (triggering/reduction of seizure frequency.
 RESULTS. Fifty patients with a verified diagnosis of epilepsy were enrolled in the study. According to study findings, 30 % of patients with childhood-onset epilepsy were not allowed to participate in physical activity when they were in school, and the majority of them were forced to give up their sports after developing epilepsy. It was also found that when the seizures were controlled, the children tried to match their peers in terms of physical activity and participated in classes despite being exempted from physical education and some continued to play sports. The results showed that in 70 % of the cases physical activity did not have negative effect on the course of the disease and in 26 % of the cases the patients reported a positive effect of regular physical activity on the course of the disease.
 CONCLUSION. Therefore, patients continued to exercise despite severe limitations in the compensated course of the disease, as physical activity with the right type and level of sport has a positive effect on the somatic and psychological state of patients with epilepsy and physical activity is chosen.

Assessment of Motor Activity of Patients Undergoing Medical Rehabilitation and Health Resort Treatment

INTRODUCTION. Problem of evaluation of the motor activity patients undergoing medical rehabilitation and sanatorium treatment remains relevant, including obesity.
 AIM. To study the nature and degree of disorders of muscle strength, motor and coordination functions in patients undergoing medical rehabilitation and sanatorium treatment.
 MATERIALS AND METHODS. Single cross-sectional study included 160 patients aged 40 to 65 years with normal and overweight. The complex of the study included: functional tests and assess muscle strength and balance.
 RESULTS. In patients with obesity, compared with persons with normal body weight of the same age, significantly (p 0.05) lower indicators of muscle strength of the right and left arms, strength of the abdominal and back muscles, lower endurance of the abdominal muscles and back muscles and longer time to complete the “Get up and walk” test. Also, in obesity, it turned out to be significantly less time to maintain balance in the “Stand on one leg” tests on the right and left legs with open eyes. A statistically significant direct relationship was found between the level of back muscle strength (γ = −0.82, p = 0.0038) and body weight. A significant relationship was also found between the level of endurance of the back muscles to physical activity and BMI (γ = −0.79, p = 0.01). At the same time, there was no relationship between age and the level of endurance of the back muscles to physical activity (γ = 0.107, p = 0.36).
 DISCUSSION. In patients undergoing medical rehabilitation and health resort treatment, obesity is associated with a decrease in muscle strength and motor activity.
 CONCLUSION. In patients with obesity at the age of 40–65 years, compared with persons with normal body weight of the same age and gender, there is a statistically significant decrease in the muscle strength of the arms, abdomen and back, a longer time to complete the “Get up” test and walk”, as well as deterioration in the function of static balance according to the results of the “Stand on one leg” test.

Motor rehabilitation after stroke: European Stroke Organisation (ESO) consensus-based definition and guiding framework.

To propose a consensus-based definition and framework for motor rehabilitation after stroke. An expert European working group reviewed the literature, attaining internal consensus after external feedback. Motor rehabilitation is defined as a process that engages people with stroke to benefit their motor function, activity capacity and performance in daily life. It is necessary for people with residual motor disability whose goal is to enhance their functioning, independence and participation. Motor rehabilitation operates through learning- and use-dependent mechanisms. The trajectory of motor recovery varies across patients and stages of recovery. Early behavioral restitution of motor function depends on spontaneous biological mechanisms. Further improvements in activities of daily living are achieved by compensations. Motor rehabilitation is guided by regular assessment of motor function and activity using consensus-based measures, including patient-reported outcomes. Results are discussed with the patient and their carers to set personal goals. During motor rehabilitation patients learn to optimize and adapt their motor, sensory and cognitive functioning through appropriately dosed repetitive, goal-oriented, progressive, task- and context-specific training. Motor rehabilitation supports people with stroke to maximize health, well-being and quality of life. The framework describes the International Classification of Functioning, Disability and Health in the context of stroke, describes neurobiological mechanisms of behavioral restitution and compensation, and summarizes recommendations for clinical assessment, prediction tools, and motor interventions with strong recommendations from clinical practice guidelines (2016-2022). This definition and framework may guide clinical educators, inform clinicians on current recommendations and guidelines, and identify gaps in the evidence base.

Spontaneously hypertensive rats vs. Wistar Kyoto and Wistar rats: An assessment of anxiety, motor activity, memory performance, and seizure susceptibility

Spontaneously hypertensive rats (SHRs) are widely accepted for modeling essential hypertension and Attention deficit hyperactivity disorder (ADHD). However, data concerning central nervous system changes associated with behavioral responses of this strain and usage of Wistar Kyoto (WKY) rats as controls are confounding. The objective of the present study was to assess the impact of anxiety and motor activity on the cognitive responses of SHRs compared to Wistar and WKY rats. In addition, the role of brain-derived neurotrophic factor (BDNF) in the hippocampus on cognitive behavior and seizure susceptibility in the three strains was evaluated. In Experiment#1, SHR demonstrated impulsive responses in the novelty suppression feeding test accompanied by impaired spatial working and associative memory in the Y maze and object recognition test compared with the Wistar rat but not WKY rats. In addition, the WKY rats exhibited diminished activity compared to Wistar rats in an actimeter. In Experiment#2, the seizure susceptibility was assessed by 3-min electroencephalographic (EEG) recording after two consecutive injections of pentylenetetrazol (PTZ) (20+40 mg/kg). The WKY rats were more vulnerable to rhythmic metrazol activity (RMA) than the Wistar rats. In contrast, Wistar rats were more prone to generalized tonic-clonic seizures (GTCS) than WKY rats and SHRs. Control SHR had lower BDNF expression in the hippocampus compared to Wistar rats. However, while the BDNF levels were elevated in the Wistar and WKY rats after PTZ injection, no change in this signaling molecule was observed in the SHR in the seizure condition. The results suggest Wistar rats as a more appropriate control of SHR than WKY rats for studying memory responses mediated by BDNF in the hippocampus. The higher vulnerability to seizures in Wistar and WKY rats compared to SHR might be linked to PTZ-induced decreased expression of BDNF in the hippocampus.

The results of the use of the telescopic intramedullary system in the surgical treatment of patients with osteogenesis imperfecta types I and III

Background. The main orthopedic manifestation of osteogenesis imperfecta is multiple fractures. In severe disease, frequent fractures lead to malunion and deformity, which increases the risk of refracture. Surgical treatment of children with osteogenesis imperfecta is aimed at reducing the risk of complications and improving the quality of life of the child. The Fassier-Duval telescopic intramedullary system has taken the leading place among the growing metal fixators. In this study, we conduct a comparative analysis of the results of a longitudinal study between patients with osteogenesis imperfecta types I and III, as well as an assessment of motor activity.
 Aim. To evaluate the efficiency of using the telescopic intramedullary system depending on the type of osteogenesis imperfecta.
 Materials and methods. A prospective analysis of surgical treatment of 20 children with types I and III was carried out. Group I included 8 patients with type I NO, group II included 12 with type III. The average age of patients was 8.2 years. Surgical treatment was carried out on the basis of the neuroorthopedic department with orthopedics of the National Medical Research Center for Children's Health. A total of 48 intramedullary telescopic pins were installed. The average period of postoperative dynamic follow-up was 42 months. The analysis of the results of surgical treatment was carried out according to the following criteria: the frequency of migrations of metal structures, the formation of bone deformities, the number of revisions, as well as the number of bone fractures with an installed metal fixator. Evaluation of the results of motor activity was carried out using two scales: Gillette Functional Assesment Questionnaire (Gillette FAQ) and HofferBullock.
 Results. There were no statistical differences in the number of complications in the two groups. Fractures among patients with type I occurred more often than in patients with type III by 19.3%. The frequency of migrations in the I study group was 7.1% (1 segment), in the II group 35.3% (12 segments). The formation of deformation was the main factor in the revision intervention, which in our case amounted to 83.3%. In the group of patients with type I, the number of repeated operations was 7.1% (1 segment in 1 patient), in the group of patients with type III 17.6% (6 segments in 4 patients). All patients in the postoperative period had II or I level of motor capabilities on the HofferBullock scale. According to the Gillette FAQ scale in the postoperative period, the subjects showed a result above 4 points.
 Conclusion. The Fassier-Duval telescopic rod is a modern and reliable metal fixator. Despite the absence of statistical differences in the two study groups (p-value20.005), there was an increase in the tendency to complications in severe course with NO. Correction of varus deformation when restoring normal values of DN125 and HEA within 2538 is the prevention of the re-formation of the truth of Coxa Vara. The motor activity of children with severe course did not statistically differ from children with mild course (p-value20.005) in the postoperative period with an average follow-up period of 42 months, regardless of the initial low motor capabilities in children with type III.

Analysis of movement activity of higher education students in the conditions of distance education

In the recent period, students of institutions of higher education (HEI) carry out educational activities with the help of remote forms of education, which is an unfavorable factor that encourages them to lead a sedentary lifestyle. Scientists testify that the motor activity of modern student youth is decreasing. However, for now, there is a lack of basic scientific investigations that would cover a long period of time, which determined the choice of the research topic. The purpose of the study was to study and analyze the dynamics of motor activity of students of higher education in the conditions of distance learning. 1528 applicants of higher education of Ukraine participated in the study, which lasted from 2019 to 2023, of which 45.2% were students. The average scores of students' motor activity during the research period were determined and the dynamics of the indicator were studied in distance learning conditions, depending on gender. It was established that regardless of gender, in 2020 there was a sharp decrease in the physical activity of students of higher education institutions due to quarantine restrictions. In 2021, student youth adapted to the difficult conditions of life and the level of their motor activity increased to the indicators of 2019. At the same time, the beginning of hostilities on the territory of the country was marked by a significant decrease in motor activity, which, unfortunately, continues. Despite a similar trend in the dynamics of motor activity, in 2023, the assessment of motor activity did not differ statistically significantly (p>0.05) compared to 2022, and in female students it decreased statistically significantly (p<0.05). It was determined that the share of students with signs of hypodynamia against the background of continuing distance learning and deteriorating living conditions is increasing and in 2023 reached 43.1% among male students and 60.1% among female students. Consequently, adverse living conditions in general and distance learning in particular increase the risk of developing hypodynamia in students of higher education. It is necessary to develop and implement measures aimed at overcoming the negative tendency to decrease the physical activity of students of higher education.

Indicators of The Dynamics of Perinatal Hypoxia in Newborns

A comprehensive study of clinical and neurological changes in newborns in the relationship between the nature and duration of perinatal hypoxia (PH) was carried out. To assess the state of clinical and neurological changes, 52 newborns who underwent perinatal hypoxia were examined. The anamnesis of mothers who underwent combined perinatal hypoxia was significantly worse than in the group of children with acute asphyxia. Anemia, acute respiratory viral infection (ARVI), preeclampsia, fetoplacental insufficiency (FPI), the duration of the anhydrous interval, pathological childbirth served as predisposing factors contributing to the development of perinatal hypoxia. For an objective quantitative assessment of the dynamics of the neurological status of newborns, the “Scoring of the severity of perinatal encephalopathy”, developed at the Research Institute of Pediatrics of the Russian Academy of Medical Sciences by A.A. Stepanov (1998), was used. It is based on an integrated assessment of motor activity, innate reflexes and behavioral reactions of newborns, taking into account the quantitative characteristics of each newborn. The data obtained indicate that the duration of hypoxia, the state of health, the course of pregnancy and childbirth in the mother determine the severity of perinatal brain damage and affect the severity of clinical and neurological patterns. Combined perinatal hypoxia leads to deeper brain disorders and contributes to the formation of two or more syndromes in one child.

The Clinical Management of Deconditioned Patient.

The current study was designed to investigate the clinical management of deconditioned patients. In the current study, we investigate the clinical manifestations of deconditioned patients. A subacute functional deficiency was determined when getting up from a supine position and developing tachycardia with an increase in heart rate by 30 or more per minute for 10 minutes. This study included 172 elderly patients aged 65 to 74 years. The average age of the patients was 69.2+2.2 years. There were 102 men and 70 women in the study. These patients were registered at the dispensary with the therapists of outpatient clinics after an infectious disease. Within 6 months after the treatment, a comprehensive geriatric assessment of patients was performed. All patients were divided into four groups depending on the indicators of motor activity, which in the framework of this study was the criterion of the adaptive function of the body and viability. Patients with normal motor activity indicators did not have the decondition and were designated as a control group. Patients with the decondition did not have normal indicators of motor activity. The obtained results were processed statistically. According to the results of our study, it was noted that the assessment of the six months after the treatment showed heterogeneity of patients from a functional point of view with a homogeneous somatic structure. Six months after the treatment, out of 172 people, 45 people (26.2%) had normal motor activity indicators (39-40 points); according to the questionnaire "Assessment of motor activity in the elderly", the average score was 39.6+0.2; 42 people (24.4%) had mild motor activity disorders (34-38 points), the average score was 36.1+0.4; moderate disorders (21-33 points) were registered in 47 people (27.3%), the average score was 27.9+2.1; significant motor activity disorders (0-20 points) occurred in 38 people (22.1%), the average score was 13.2+1.4. The fall syndrome was characteristic of those patients with the decondition who demonstrated moderate and significant motor activity disorders 6 months after the treatment. In particular, in people with significant motor activity disorders, there was a decrease in muscle strength in the lower extremities by 48.3+3.2% and in the upper extremities - by 27.1+3.1% (P<0.05). As a result of the presence of the decondition, an unfavorable cascade of geriatric syndromes is formed in the form of an increase in the level of situational anxiety from 21.9-24.8 to 58.4-75.3 points on the Spielberger-Hanin scale, a deterioration in morale with an increase in the severity of depression from 5.8-6.0 to 11.0-14.9 points, a decrease in the quality of sleep on the ten-point visual analog scale from 8.3-8.4 to 5.2-5.7 points on the Beck scale, an increase in the proportion of patients at risk of developing malnutrition syndrome from 4.8-6.7% to 46.8-68.4%, the development of the fall syndrome, which, in turn,, it is a predictor of the development of dinapenia in the form of a decrease in the strength of the upper extremities by 22.7-27.1%, a decrease in the strength of the lower extremities by 29.6-49.6%, with a faster rate of decrease in the strength of the lower extremities compared to the upper extremities by 1.8 times, which, accordingly, reduces the rehabilitation potential. Clinical manifestations of the decondition in elderly patients were such geriatric syndromes as anxiety, depression, cognitive decline, sleep disorders, hypomobility, dinapenia, risk of developing malnutrition syndrome and falls syndrome.

Objective assessment of motor activity in a clinical sample of adults with attention-deficit/hyperactivity disorder and/or cyclothymic temperament

BackgroundMost research on patterns of motor activity has been conducted on adults with mood disorders, but few studies have investigated comorbid attention-deficit/hyperactivity disorder (ADHD) or temperamental factors that may influence the clinical course and symptoms. Cyclothymic temperament (CT) is particularly associated with functional impairment. Clinical features define both disorders, but objective, biological markers for these disorders could give important insights with regard to pathophysiology and classification.MethodsSeventy-six patients, requiring diagnostic evaluation of ADHD, mood or anxiety disorders were recruited. A comprehensive diagnostic evaluation, including the CT scale of the Temperament Evaluation of Memphis, Pisa, Paris and San Diego – Auto-questionnaire (TEMPS-A), neuropsychological tests and actigraphy, was performed. ADHD was diagnosed according to the DSM-IV criteria. There was a range of different conditions in this clinical sample, but here we report on the presence of CT and ADHD in relation to motor activity. Twenty-nine healthy controls were recruited. We analyzed motor activity time series using linear and nonlinear mathematical methods, with a special focus on active and inactive periods in the actigraphic recordings.ResultsForty patients fulfilled the criteria for ADHD, with the remainder receiving other psychiatric diagnoses (clinical controls). Forty-two patients fulfilled the criteria for CT. Twenty-two patients fulfilled the criteria for ADHD and CT, 18 patients met the criteria for ADHD without CT, and 15 patients had neither. The ratio duration of active/inactive periods was significantly lower in patients with CT than in patients without CT, in both the total sample, and in the ADHD subsample.ConclusionsCT is associated with objectively assessed changes in motor activity, implying that the systems regulating motor behavior in these patients are different from both healthy controls and clinical controls without CT. Findings suggest that actigraphy may supplement clinical assessments of CT and ADHD, and may provide an objective marker for CT.

Unique Neural Circuit Connectivity of Mouse Proximal, Middle, and Distal Colon Defines Regional Colonic Motor Patterns.

Background & AimsColonic motor patterns have been described by a number of different groups, but the neural connectivity and ganglion architecture supporting patterned motor activity have not been elucidated. Our goals were to describe quantitatively, by region, the structural architecture of the mouse enteric nervous system and use functional calcium imaging, pharmacology, and electrical stimulation to show regional underpinnings of different motor patterns.MethodsExcised colon segments from mice expressing the calcium indicator GCaMP6f or GCaMP6s were used to examine spontaneous and evoked (pharmacologic or electrical) changes in GCaMP-mediated fluorescence and coupled with assessment of colonic motor activity, immunohistochemistry, and confocal imaging. Three-dimensional image reconstruction and statistical methods were used to describe quantitatively mouse colon myenteric ganglion structure, neural and vascular network patterning, and neural connectivity.ResultsIn intact colon, regionally specific myenteric ganglion size, architecture, and neural circuit connectivity patterns along with neurotransmitter-receptor expression underlie colonic motor patterns that define functional differences along the colon. Region-specific effects on spontaneous, evoked, and chemically induced neural activity contribute to regional motor patterns, as does intraganglionic functional connectivity. We provide direct evidence of neural circuit structural and functional regional differences that have only been inferred in previous investigations. We include regional comparisons between quantitative measures in mouse and human colon that represent an important advance in showing the usefulness and relevance of the mouse system for translation to the human colon.ConclusionsThere are several neural mechanisms dependent on myenteric ganglion architecture and functional connectivity that underlie neurogenic control of patterned motor function in the mouse colon.

Evaluation of socialization technology effectiveness in motor-play activity of preschoolers

Motor-play activity is the basis for the social experience recreation and development among older preschoolers, a condition for positive socialization. The relevance of the research is conditioned by the need to comprehend the potential of socialization technology in motor-game activity of preschoolers. The use of technology in the educational process at kindergartens contributes to the complex impact on a child, the development of his physical, social, communicative and personal potentials. The purpose of the study is to assess the effectiveness of socialization technology in motor-play activity among 5-7-year-old children. Study methods: theoretical: analysis, synthesis, generalization; empirical: observation and assessment of independent motor activity among children, game diagnostic situations, conversation; the methods of mathematical statistics: Wilcoxon t-test. The study involved 120 senior preschool children attending the kindergarten No. 78 in the city of Belgorod, No. 15 in the village of Razumnoe, Belgorodsky District, Belgorod Region. The analysis of the study results showed that in the process of socialization technology implementation in motor-play activity among preschoolers, 95% of children who participated in the experiment use successfully the social experience in motor-play activity and show the optimal and sufficient level of its recreation.

CAPABILITIES OF AN INFORMATION-ANALYTICAL SYSTEM FOR ASSESSING MOTOR ACTIVITY IN PARKINSON'S DISEASE DURING SLEEP

The research aimed to study motor activity in Parkinson's disease during sleep using a data analytical system developed by authors. The study included 8 patients (4 men and 4 women) diagnosed with Parkinson's disease at the age from 59 to 89 years. All patients were clinically evaluated for motor deficit (UPDRS Part III), motor fluctuations (UPDRS Part IV), functional activity (UPDRS Part I), non-motor symptoms (UPDRS Part II) and the clinical global impression. Using our information-analytical system we obtained data on motor activity during sleep and processed it on the following parameters: number of movements, maximum value of the jerk and motor activity coefficient. For the number of movements, the average value for men was 56.7, for women — 47.2. For the maximum value of the jerk, the average value for men was 22.1 g/s, for women — 16.7 g/s. For motor activity coefficient, the average value for men was 27.7%, for women — 22.3%. Thus, the feasibility of using the information analytical system for assessment of motor activity in Parkinson's disease during sleep and the necessity of studying hypokinetic tremors during sleep has been shown.

Antinociceptive and anti-inflammatory activities of Copaifera pubiflora Benth oleoresin and its major metabolite ent-hardwickiic acid

Ethnopharmacological relevanceCopaifera species folkloric names are “copaíbas, copaibeiras, copaívas or oil stick”, which are widely used in Brazilian folk medicine. Among all ethnopharmacological applications described for Copaifera spp oleoresins, their anti-inflammatory effect stands out. However, the knowledge of anti-inflammatory and antinociceptive properties of Copaifera pubiflora Benth is scarce. Aim of the studyTo investigate the cytotoxic, anti-inflammatory, and antinociceptive activities of C. pubiflora oleoresin (CPO), and its major compound ent-hardwickiic acid (HA). Material and methodsThe phosphatase assay was used to evaluate the cytotoxicity of CPO and HA in three different cell lines. CPO and HA doses of 1, 3, and 10 mg/kg were employed in the biological assays. The assessment of motor activity was performed using open-field and rotarod tests. Anti-inflammatory activity of CPO and HA was assessed through luciferase assay, measurement of INF-γ, IL-1β, IL-6, IL-10, and TNF-α in a multi-spot system with the immortalized cell line THP-1, zymosan-induced arthritis, and carrageenan-induced paw edema. Acetic acid-induced abdominal writhing and formalin tests were undertaken to evaluate the antinociceptive potential of CPO and HA. In addition, the evaluation using carrageenan was performed to investigate the effect of CPO in pain intensity to a mechanical stimulus (mechanical hyperalgesia), using the von Frey filaments. A tail-flick test was used to evaluate possible central CPO and HA actions. ResultsIn the cytotoxicity evaluation, CPO and HA were not cytotoxic to the cell lines tested. CPO and HA (10 mg/kg) did not affect animals' locomotor capacity in both open-field and rotarod tests. In the luciferase assay, CPO and HA significantly reduced luciferase activity (p < 0.05). This reduction indicates a decrease in NF-κB activity. HA and CPO decreased INF-γ, IL-1β, IL-6, IL-10, and TNF-α at 24 and 72 h in the multi-spot system. In zymosan-induced arthritis, CPO and HA decreased the number of neutrophils in the joint of arthritic mice and the number of total leukocytes (p < 0.05). In experimental arthritis HA significantly decreased joint swelling (p < 0.05). CPO and HA also increased the mechanical threshold during experimental arthritis. HA and CPO significantly inhibited the carrageenan-induced paw edema, being the doses of 10 mg/kg the most effective, registering maximum inhibitions of 58 ± 8% and 76 ± 6% respectively, p < 0.05. CPO and HA reduced the nociceptive behavior in both phases of formalin at all tested doses. The highest doses tested displayed inhibitions of 87 ± 1% and 72 ± 4%, respectively, p < 0.001, in the first phase, and 87 ± 1% and 81 ± 2%, respectively, p < 0.001, in the second phase. Oral treatment of CPO and HA (1, 3, 10 mg/kg) significantly reduced the nociceptive response in acetic acid-induced abdominal writhings, and the 10 mg/kg dose was the most effective with maximum inhibitions of 86 ± 2% and 82 ± 1%, respectively, p < 0.001. Both HA and CPO significantly decreased the intensity of mechanical inflammatory hyper-nociception on carrageenan-induced hyperalgesia at all tested doses, and 10 mg/kg was the most effective dose with maximum inhibitions of 73 ± 5% and 74 ± 7%, respectively, p < 0.05.CPO increased the tail-flick latencies in mice, and concomitant administration of naloxone partially reduced its effect. ConclusionsCPO and HA may inhibit the production of inflammatory cytokines by suppressing the NF-κB signaling pathway, resulting in anti-inflammatory and antinociceptive activities.

PROSPECTIVE PILOT STUDY TO ASSESS MOTOR ACTIVITY AND THE STATE OF THE HEMOSTASIS SYSTEM IN THE ACUTE PERIOD OF ISCHEMIC STROKE DURING SYSTEMIC THROMBOLYTIC THERAPY

The aim: To investigate changes in motor activity and indicators of the state of the hemostasis system in the acute period of ischemic stroke during systemic thrombolytic therapy and without its use. Materials and methods: We examined 26 male and female patients with a clinical diagnosis of ischemic stroke, who were hospitalized on the first day of the disease to the neurological departments. Patients were divided into 2 groups: group 1-patients who underwent systemic thrombolytic therapy (sTLT) (n=11), group 2-patients who did not receive sTLT (n=15). To compare the coagulogram parameters, 12 healthy patients were examined (control group). Examination of patients was performed on the 1st and 14th day of the disease (clinical examination, assessment of motor activity, coagulation test). Stroke severity was determined by the overall score of the National Institutes of Health Stroke Scale. Results: The average age of patients in group 1 - 60.1±8.2 years old, in group 2 -61.3±5.5 years old. The number of points on the NIHSS scale in group 1 was 8.8±1.13 on 1st day and 3.7±0.79 on 14th day (p<0.05), in group 2 -5.7±0,94 on the 1st day and 3.1±0.93 on the 14th day(p<0.05). The results of the study of the coagulogram indicate a significantly higher level of soluble fibrin-monomer complexes on the 1st day on the 14th day of the ischemic stroke. Conclusions: In the acute period of ischemic stroke changes in the hemostasis system reflected the direction of the selected therapy. The use of systematic thrombolytic therapy in ischemic stroke led to a more severe decrease in stroke severity on the NIHSS scale, a significant increase in Barthel index.

Assessment of motor activity of knowledge workers (on the example of employees of social service centers)

Introduction. The work of many employees in the service sector is associated with a constant stay in a forced working position sitting at the computer for up to 60-80% of the time of the entire work shift, which leads to fatigue. The rate of fatigue depends on the specifics of work: it is much more likely to occur when working with monotony, muscle tension. An important role in the appearance of fatigue is also played by the attitude of a person to the work performed. The aim of study is to study the motor activity of knowledge workers in social service centers for the development of preventive measures aimed at reducing hypokinesia and hypodynamia during the work shift. Materials and methods. The following methods were used in the study: questionnaire and working day timing, calculation methods to determine whether the actual body weight corresponds to the ideal one using the Broka’s index and the Ketle’s index. The study involved employees of the integrated social service center "Raduga" in Bugulma. Results. An assessment of the motor activity of employees of the social service center of the population was made, and it was concluded that the problem of hypokinesia and hypodynamia is relevant and significant for employees of social service centers of the population both in their daily activities and during the work shift. Most of the employees showed an increase in actual body weight compared to the ideal one, which is to some extent due to hypokinesia and hypodynamia both during the working day and at home. Conclusions. Preventive measures aimed at compliance with the principles of rational nutrition and a healthy lifestyle, work and rest regimes, high-quality preliminary and periodic medical examinations are proposed.

Evaluating the Utility of Smartphone-Based Sensor Assessments in Persons With Multiple Sclerosis in the Real-World Using an App (elevateMS): Observational, Prospective Pilot Digital Health Study.

BackgroundMultiple sclerosis (MS) is a chronic neurodegenerative disease. Current monitoring practices predominantly rely on brief and infrequent assessments, which may not be representative of the real-world patient experience. Smartphone technology provides an opportunity to assess people’s daily-lived experience of MS on a frequent, regular basis outside of episodic clinical evaluations.ObjectiveThe objectives of this study were to evaluate the feasibility and utility of capturing real-world MS-related health data remotely using a smartphone app, “elevateMS,” to investigate the associations between self-reported MS severity and sensor-based active functional tests measurements, and the impact of local weather conditions on disease burden.MethodsThis was a 12-week, observational, digital health study involving 3 cohorts: self-referred participants who reported an MS diagnosis, clinic-referred participants with neurologist-confirmed MS, and participants without MS (controls). Participants downloaded the elevateMS app and completed baseline assessments, including self-reported physical ability (Patient-Determined Disease Steps [PDDS]), as well as longitudinal assessments of quality of life (Quality of Life in Neurological Disorders [Neuro-QoL] Cognitive, Upper Extremity, and Lower Extremity Function) and daily health (MS symptoms, triggers, health, mobility, pain). Participants also completed functional tests (finger-tapping, walk and balance, voice-based Digit Symbol Substitution Test [DSST], and finger-to-nose) as an independent assessment of MS-related cognition and motor activity. Local weather data were collected each time participants completed an active task. Associations between self-reported baseline/longitudinal assessments, functional tests, and weather were evaluated using linear (for cross-sectional data) and mixed-effects (for longitudinal data) regression models.ResultsA total of 660 individuals enrolled in the study; 31 withdrew, 495 had MS (n=359 self-referred, n=136 clinic-referred), and 134 were controls. Participation was highest in clinic-referred versus self-referred participants (median retention: 25.5 vs 7.0 days). The top 5 most common MS symptoms, reported at least once by participants with MS, were fatigue (310/495, 62.6%), weakness (222/495, 44.8%), memory/attention issues (209/495, 42.2%), and difficulty walking (205/495, 41.4%), and the most common triggers were high ambient temperature (259/495, 52.3%), stress (250/495, 50.5%), and late bedtime (221/495, 44.6%). Baseline PDDS was significantly associated with functional test performance in participants with MS (mixed model–based estimate of most significant feature across functional tests [β]: finger-tapping: β=–43.64, P<.001; DSST: β=–5.47, P=.005; walk and balance: β=–.39, P=.001; finger-to-nose: β=.01, P=.01). Longitudinal Neuro-QoL scores were also significantly associated with functional tests (finger-tapping with Upper Extremity Function: β=.40, P<.001; walk and balance with Lower Extremity Function: β=–99.18, P=.02; DSST with Cognitive Function: β=1.60, P=.03). Finally, local temperature was significantly associated with participants’ test performance (finger-tapping: β=–.14, P<.001; DSST: β=–.06, P=.009; finger-to-nose: β=–53.88, P<.001).ConclusionsThe elevateMS study app captured the real-world experience of MS, characterized some MS symptoms, and assessed the impact of environmental factors on symptom severity. Our study provides further evidence that supports smartphone app use to monitor MS with both active assessments and patient-reported measures of disease burden. App-based tracking may provide unique and timely real-world data for clinicians and patients, resulting in improved disease insights and management.

INDIVIDUAL-TYPOLOGICAL APPROACH TO THE ASSESSMENT OF MOTOR ACTIVITY AND FUNCTIONAL STATE OF SENIOR PRESCHOOL AND JUNIOR SCHOOL CHILDREN

Physical activity is of particular importance in the early stages of ontogenesis during the development and formation of the child's body. At the same time, recognizing the variability of motor activity in children of a healthy population, its assessment in the understanding of the norm is presented as an average indicator of the daily number of locomotions for a certain age and gender group, which does not allow us to fully characterize the individual typological features of this function. In this regard, the use of signal parametric scales is very controversial and new approaches to solving this problem are required. The aim of the work is to conceptually develop the centile distribution and partial density of motor activity in older preschoolers and younger schoolchildren in a healthy population with the identification of typological variants of individual norms and criteria for pre - nosological diagnosis of hyper-and hypokinesia. Materials and methods. 840 children of health groups I-II were examined (boys of senior preschool age-222 and primary school age-250; girls of senior preschool age-181 and primary school age-187). The study included the use of pedometry and visual assessment of children's behavior by teachers and parents. A personalized database was created based on the survey results. To identify differences in the level of distribution of a typical trait – habitual motor activity (HMA), a nonparametric test was used, taking into account Pearson's χ2 criterion. The functional type of constitution (FTC) was determined in accordance with a three – component scheme based on the level of habitual motor activity: low – LHMA-FTC–1, medium – MHMA–FTC–2 and high –HHMA-FTC–3. To solve this problem, we developed centile tables based on the daily number of locomotives (SCL) - the HMA level. At the same time, tables are proposed for the centile distribution of SCL both for the entire population of preschool and primary school children, and for children with low and high motor activity. Results. Analysis of the data and on this basis developed normative tables of centile distribution of motor activity (MA) allow typing of it is high enough differences among senior preschool children and Junior schoolchildren of the healthy population with the estimated number of daily locomotion (very low, low, medium, high, very high), to allocate the risk group of I order (up to the 3-rd centile and 97-th centile) and II (from the 3-rd to the 10-th centile and the 90-th to 97-th centile), and also give an objective description of the daily number of locomotions of each individual with the identification of such concepts as "optimal motor activity", "constitutional hypokinesia" and "constitutional hyperkinesia". Partial assessment of motor activity, taking into account the centile distribution of the number of daily locomotions in each constitutional group, provides additional opportunities to more reasonably approach the analysis of a critical (pre-nosological) decrease or increase in the optimality of motor activity in children with low and high MA. Conclusions. Senior preschool children and Junior school children of health groups I-II have their own individual and typological basic level of motor activity, which requires a comparative clinical and physiological analysis of the available data with the standard norm characteristic of each constitutional group (LHMA-FTC–1, MHMA-FTC–2, HHMA-FTC–3). Taking into account this approach, the development of normative (centile) tables of motor activity allows us to give not only a quantitative assessment of the basic variability of the physiological individuality of the child's body, but also to identify criteria for pre-nosological diagnosis of fairly common deviations in pediatric practice-hypokinesia and hyperactivity.