Community Assessment Research Articles

Integrating citizen science and environmental DNA metabarcoding to study biodiversity of groundwater amphipods in Switzerland

Groundwater is the physically largest freshwater ecosystem, yet one of the least explored habitats on earth, both because of accessing difficulties and the scarcity of the organisms inhabiting it. Here, we demonstrate how a two-fold approach provides complementary information on the occurrence and diversity of groundwater amphipods. Firstly, we used a citizen science approach in collaboration with municipal water providers who sampled groundwater organisms in their spring catchment boxes over multiple weeks, followed by DNA barcoding. Secondly, we collected four 10 L water samples at each site, in one sampling event, for environmental DNA (eDNA) metabarcoding. We found that citizen science was very effective in describing the distribution and abundance of groundwater amphipods. Although the single time-point of eDNA sampling did not detect as many amphipods, it allowed the assessment of the entire groundwater community, including microorganisms. By combining both methods, we found different amphipod species co-occurring with distinct sequences from the eDNA-metabarcoding dataset, representing mainly micro-eukaryotic species. We also found a distinct correlation between the diversity of amphipods and the overall biodiversity of groundwater organisms detected by eDNA at each site. We thus suggest that these approaches can be used to get a better understanding of subterranean biodiversity.

The provision of malaria services in border districts of four countries in Southern Africa: results from a cross-sectional community assessment of malaria border health posts

BackgroundThe importation of parasites across borders remains a threat to malaria elimination. The Southern African Development Community Malaria Elimination Eight (E8) established 39 border health facilities on 5 key international borders between high and low-burden countries. These clinics aimed to improve access to prevention, diagnosis, and treatment of malaria for residents in border areas and for mobile and migrant populations who frequently cross borders. Studies were conducted in each of the four high-burden E8 countries (Angola, Mozambique, Zambia, and Zimbabwe) to evaluate malaria services in border areas.MethodsCross-sectional surveys were conducted within 30 km of recently established E8 Border Health Posts. Structured questionnaires were administered to randomly selected respondents to assess malaria-related knowledge and behavior, access to malaria prevention, diagnosis and treatment of malaria, and risk factors for malaria associated with local and cross-border travel.ResultsResults showed that most providers followed appropriate guidelines performing blood tests when individuals presented with fever, and that nearly all those who reported a positive blood test received medication. Lack of access to health care due to distance, cost or mistrust of the provider was rare. A minority of respondents reported not receiving timely diagnosis either because they did not seek help, or because they were not offered a blood test when presenting with fever. There was a high level of correct knowledge of causes, symptoms, and prevention of malaria. A majority, of border residents had access to primary prevention against malaria through either long-lasting insecticidal nets (LLINs) or indoor residual spraying (IRS). Cross border travel was common with travellers reporting sleeping outside without protection against malaria.ConclusionsThe study demonstrated the importance of border health facilities in providing access to malaria services. Prevention needs to be improved for people who travel and sleep outdoors. Community health workers can play a key role in providing access to information, testing and treating malaria.

Acceptance and Commitment Therapy and white matter plasticity in individuals with subclinical depression and psychotic experiences: A Randomised Controlled Trial

BackgroundResearch indicates that Acceptance and Commitment Therapy in Daily Life (ACT-DL) is effective in reducing symptoms of depression, anxiety and psychosis. During adolescence, vulnerability to psychopathology peaks, creating a window for early interventions, while white matter development is ongoing. This study aims to examine microstructural white matter after ACT-DL intervention in youngsters with mild psychopathology. MethodsForty-five individuals with mild psychopathology were randomly allocated to ACT-DL (n=20) or topic discussion control (TD, n=25). Symptomatology was assessed with the Community Assessment of Psychic Experiences (CAPE), Montgomery–Åsberg Depression Rating Scale (MADRS) and the Experience Sampling Method (ESM). Diffusion Weighted Imaging (DWI) and network-connectivity parameters were obtained and compared before and after the intervention/control condition. Interactions between microstructural white matter change and condition were examined in models of CAPE positive symptoms and ESM subclinical psychotic experiences (PE) and negative affect (NA) levels. ResultsACT-DL, compared to TD, was associated with changes on subclinical depressive and psychotic symptom levels. There was no significant change in DWI or network connectivity in either condition and no significant difference between both conditions. In the model of NA, several regional interactions between condition and network measures were significant, but stratification per condition provided no significant associations. There were no significant interactions between DWI or network connectivity parameters and condition in the models of the CAPE positive symptoms, MADRS and PE. ConclusionsThe findings suggest that behavioral (symptom) changes are more sensitive to a five-week psychological training than microstructural white matter changes which did not show significant changes over time.

Testing the efficacy of the Heslinga live trap for small mammal community assessments

Testing the efficacy of the Heslinga live trap for small mammal community assessments

Depression and anxiety mediate the relationship between the retrospectively measured symptoms of premenstrual disorders and negative but not positive psychotic-like experiences

The aim of this study was to examine the relationship between retrospectively measured premenstrual symptoms and subclinical forms of positive and negative psychotic symptoms (psychotic-like experiences; PLEs). It was hypothesised that subjective intensity of the symptoms of premenstrual disorders predicts PLEs frequency and that this relationship is mediated by anxiety and depression. The study sample comprised 108 non-clinical subjects. Study variables were assessed with self-report questionnaires: the Premenstrual Symptoms Screening Tool (PSST); the Beck Depression Inventory Second Edition (BDI-II); the State-Trait Anxiety Inventory (STAI; trait sub-scale); the Community Assessment of Psychic Experiences (CAPE). Regression and mediation analyses were performed. The PSST scores were significantly and positively associated with psychotic-like experiences frequency. The relation was stronger for positive PLEs. Anxiety and depression partially mediated the relationship between premenstrual symptoms and negative PLEs, but not between premenstrual symptoms and positive PLEs. Although the design of the study does not allow to infer causality, it demonstrates strong, positive relationship between the symptoms of premenstrual disorders and psychotic-like experiences. The relationship between premenstrual disorders and negative PLEs seems to be partially based on a general psychopathological factor. Further longitudinal studies are needed to test whether premenstrual disturbances increase risk of psychotic symptoms.

Refinement of the NISECI ecological index reference conditions for Italian freshwater fish communities in the eastern Emilia-Romagna region

Following the Water Framework Directive 2000/60/CE (WFD), each member state of the European Union must monitor compliance of its rivers with ecological quality standards through biological quality indicators. The New Italian Index of the Ecological State of Fish Communities (NISECI) was developed in 2017 for the assessment of fish communities, as directed by the WFD in Italian freshwater habitats. According to the WFD, the general reference conditions (GRCs) of NISECI must be refined on a regional scale through new calculation of its metrics and sub-metrics. In the present study we used environmental and ichthyological data from 457 fish samplings distributed among 299 sampling sites within 84 different water bodies collected from 1995 to 2012 to develop: 1) new lists of expected species for six homogeneous zones identified in the Reno basin (Italy) and in the eastern regional basins of the Emilia-Romagna region; and 2) the threshold values for their species-specific abundance. Results were set as refined reference conditions (RRCs) for two of the metrics used in the application of the NISECI index in the study area (i.e. X1, relating to indigenous species and X2,b, for the abundance of expected species). The RRCs were tested by applying NISECI to 24 monitoring sites of the regional surface water monitoring network (i.e., ARPAE) and comparing the results with the application of NISECI using the GRCs. Furthermore, the analytical power of the refined NISECI was evaluated by relating the findings to three expert-based blind assessments of fish community ecological status. The results confirmed an increase in refined NISECI values and its higher consistency with expert-based assessment, supporting the validity of the presented method for RRC development and its potential for application in other regions.

Clinical presentation of psychotic experiences in patients with common mental disorders attending the UK primary care improving access to psychological therapies (IAPT) Programme

BackgroundImproving Access to Psychological Therapies (IAPT) services address anxiety and depression in primary care, with psychotic disorders typically excluded. Our previous research found 1 in 4 patients report distressing psychotic experiences (PE) alongside common mental disorders, yet little is known about their clinical presentation and impact on recovery. MethodsWe used the Community Assessment of Psychic Experiences — Positive Scale (CAPE-P15) to assess the clinical presentation and symptomatic profile of PE within IAPT settings across three National Health Service (NHS) trusts, serving a diverse population in Southern England. We identified different classes based on the reported PE frequencies using latent class analysis. ResultsA total of 2042 IAPT patients completed the CAPE-P15. The mean age was 39.8 (±15.3) years. We identified five distinct classes of symptom profiles, findings that PE were common, especially self-referential and persecutory ideas. Prevalence and intensity increased across classes, extending to bizarre experiences and perceptual abnormalities in the fifth and least common class. Perceptual abnormalities were a strong indicator of symptom severity, with patients being the least likely to achieve recovery by the end of treatment. LimitationsData were collected during a service evaluation. Replication of these findings across other IAPT services could prove beneficial. We did not collect information on negative PE. ConclusionsPatients seeking treatment for anxiety and depression in primary care commonly experience a wide range of positive PE. Self-referential and persecutory ideation were prevalent; perceptual abnormalities were infrequent. Providing information about prevalence and tailoring therapy may help reduce patient distress.

Green matters: Dietary assessment of a reptile community using DNA metabarcoding

DNA metabarcoding is widely used for diet characterization and is becoming increasingly important for biodiversity conservation, allowing the understanding of trophic networks and community assemblies. However, to our knowledge, few studies have used this approach to investigate trophic interactions for whole communities and none for reptiles. In particular, few studies have examined the diet composition of Saudi Arabian reptiles, and all have used classical methods only. Therefore, in this work, a non-invasive approach using DNA metabarcoding of faecal pellets was implemented to investigate the diet composition of the reptile community of Wadi Ashar, in AlUla County, north-western Saudi Arabia Kingdom. In the overall diet composition of the community, arthropods were present in 90% of the samples, and plants were present in 63%, revealing the unforeseen importance of plants to this community as a secondary, but also a primary dietary item. For some species, this is the first time that plants have been reported in their diet. A significant effect of reptile body size on diet composition was also demonstrated, indicating its strong influence on prey selection and resource partitioning in the community. This study highlights the importance of community assessments and the power of combining these with non-invasive DNA metabarcoding to accurately assess biodiversity and feeding habits, revealing unknown ecological interactions of often neglected groups. This revolutionary tool for conservation and management provided rapid and holistic information at relatively low costs, allowing to inform local authorities about which elements are central to the sustainable management of the Wadi Ashar community.

Frequency of newborn bathing in the first 9 weeks of life and related factors: An observational study in a community-based sample from Meta-LARC.

Environmental factors such as bathing may play a role in atopic dermatitis (AD) development. This analysis utilized data from the Community Assessment of Skin Care, Allergies, and Eczema (CASCADE) Trial (NCT03409367), a randomized controlled trial of emollient therapy for AD prevention in the general population, to estimate bathing frequency and associated factors within the first 9 weeks of life. Data were collected from 909 parent/newborn dyads recruited from 25 pediatric and family medicine clinics from the Meta-network Learning and Research Center (Meta-LARC) practice-based research network (PBRN) consortium in Oregon, North Carolina, Colorado, and Wisconsin for the CASCADE trial. Ordinal logistic regression was used to conduct a cross-sectional analysis of the association between bathing frequency (measured in baths per week) and demographic, medical, and lifestyle information about the infant, their family, and their household. Variables were selected using a backwards-stepwise method and estimates from the reduced model are reported in the text. Moisturizer use (OR = 2.03, 95% CI: 1.54-2.68), Hispanic or Latino ethnicity (OR = 1.97, 95% CI: 1.42-2.72), a parental education level lower than a 4-year college degree (OR = 2.48, 95% CI: 1.70-3.62), living in North Carolina or Wisconsin (compared to Oregon; OR = 2.12 and 1.47, 95% CI: 1.53-2.93 and 1.04-2.08, respectively), and increasing child age (in days; OR = 1.02, 95% CI: 1.01-1.02) were significantly associated with more frequent bathing, while pet ownership (OR = 0.67, 95% CI: 0.52-0.87) was significantly associated with less frequent bathing. We found significant ethnic, geographic, and socioeconomic variation in bathing frequency before 9 weeks of age that may be of relevance to AD prevention studies.

Analisis Pengaruh Kualitas Pelayanan Publik Terhadap Kepuasan Masyarakat (Studi tentang Layanan Perizinan Online Pada Dinas Penanaman Modal dan Pelayanan Terpadu Satu Pintu Kabupaten Badung)

Community satisfaction is the result of community opinions and assessments of the performance of services provided by public service providers. Public satisfaction reflects the government's success in providing effective and efficient public services. The factor that can influence the level of public satisfaction is the quality of public services. This research aims to analyze the influence of the quality of LAPERON public services in DPMPTSP Badung Regency on the satisfaction of people using LAPERON. This research was conducted on LAPERON users with a sample size of 370 respondents. Data collection in this research was carried out using the questionnaire method. The data analysis techniques used in this research are Simple Linear Regression Test, Simple Correlation Test, Determination Test, and T Test. The results of this research correlation test get a score of 0.855, so it can be said that variables X and Y have a very strong correlation. Then the results of the coefficient of determination, namely by squaring the correlation results, produce an R-Square of 0.731, which means that the quality of public services has an influence on public satisfaction of 73.1%. So it can be concluded that H0 is rejected and H1 is accepted. There is a positive and significant influence between the quality of LAPERON public services in DPMPTSP Badung Regency on the satisfaction of the LAPERON user community. The advice for LAPERON in DPMPTSP Badung Regency is to continue to pay attention to the quality of public services so that they remain positive, which can increase public trust and satisfaction with LAPERON.

Provision of mental health and psychosocial support services to health workers and community members in conflict-affected Northwest Syria: a mixed-methods study

BackgroundNorthwest Syria (NWS) is a conflict area with challenging political, economic, demographic and social dynamics. The region has a high number of internally displaced persons with increasingly disrupted delivery of basic services, including healthcare. Mental health needs have been increasing in the region while the infrastructure and capacity of the health sector has been negatively affected by the conflict. This study aimed to explore the provision of mental health and psychosocial support (MHPSS) services to communities in NWS (including healthcare workers) and to assess the experiences of beneficiaries with MHPSS services.MethodsThe study followed a mixed-methods research design that included qualitative and participatory methods (44 semi-structured interviews and a group model building workshop with 15 participants) as well as a survey with 462 beneficiaries.ResultsFindings suggested an improvement of MHPSS services in the region over the last few years due to the creation of a specific Technical Working Group for MHPSS that contributed to assessment of community needs and support of the MHPSS response. The key elements of this response were: (1) training non-specialized health workers to address the shortage in specialized providers; (2) securing funding and coordination of services between different organizations; and (3) addressing gaps in the availability and geographical distribution of other needed resources, such as medicines. While those elements contributed to improving access to services and the quality of services—especially among health workers seeking MHPSS services—findings suggested gaps in the sustainability of services and a need to scale up those interventions in an integrated approach.ConclusionThe study findings add to the evidence base on the challenges in scaling up MHPSS interventions and their long-term sustainability concerns. Priority actions should address the intermittent funding of the MHPSS response, incorporate MHPSS outputs and outcomes in the reimbursement of routine services, improve coordination between health partners and non-health actors in order to expand the scope of MHPSS response, and address the inequitable availability of resources in the region.

RIAU PROVINCE TOURISM WEBSITE IMPROVEMENT PROGRAM USING E-GOVQUAL AND IMPORTANCE PERFORMANCE ANALYSIS METHODS

The Riau Province Tourism Office implements E-Government by building a website as a media public relations. This website is used to provide information on tourist destinations, facilities and news about Riau Province. Previously this website had never been evaluated so that the community's assessment of the quality of the Riau Province Tourism service site was unknown. For this reason, this study aims to analyze the service quality of the Riau Province Tourism Office website based on the perception or point of view of its users, in this case especially the people of Riau Province using the Electronic Government Quality (E-GovQual) and Importance Performance Analysis (IPA) methods. To assess the service quality of the Riau Province Tourism Office website using 6 dimensions and 28 E-GovQual attributes. The results of this study show that the total value of the performance score is Likert 3 (good) which is 3.30, the value of importance is 3.27, and the gap between performance and importance is 0.002 or > 0. The results of this study indicate that service performance has met the interests user. Thus the overall performance of the Riau Province Tourism Office website service is good and has fulfilled the interests of users. Furthermore, from the results of the IPA analysis, 7 attributes (EU1, TR1, FIE2, EU2, TR3, CS2, CAI3) are obtained with top priority and 5 attributes (EU3, FIE1, RB3, CA12, TR2) that need to be improved.

DNA metabarcoding assessment of Neotropical ichthyoplankton communities is marker-dependent.

The study of ichthyoplankton is paramount to understanding fish assemblages' reproductive dynamics. DNA metabarcoding has been applied as a rapid, cost-effective, and accurate taxonomy tool, allowing the identification of multiple individuals simultaneously. However, there remain significant challenges when using DNA metabarcoding, such as molecular marker choice according to the taxonomic resolution and length of the fragment to be sequenced, primer bias, incomplete reference databases, and qualitative inference incongruences. Here, 30 ichthyoplankton pools collected from a Neotropical river were identified at a molecular level using DNA metabarcoding to compare the resolution, sensibility, specificity, and relative read abundance (RRA) recovery of three molecular markers: the standard COI fragment (650 pb, with each end analyzed individually) and two short 12S rRNA genes markers (≅200 bp - NeoFish and MiFish markers). The combined use of the three markers increased the genera detection rates by 25%-87.5%, allowing an increased taxonomic coverage and robust taxonomic identification of complex Neotropical ichthyoplankton communities. RRA is marker-dependent, indicating caution is still needed while inferring species abundance based on DNA metabarcoding data when using PCR-dependent protocols.

Assessment of energy sharing coefficients under the new Portuguese renewable energy communities regulation

The recent published European legal framework (Directives (EU) 2018/2001 and 2019/944) on renewable energy consumption, and its Portuguese transposition (the Decree-Law 15/2022), opens the possibility for buildings to operate as energy communities. One of the objectives is to increase the use of locally generated energy from renewable sources, by sharing available surplus among participants, using sharing coefficients defined by the entire community.Taking the actual legal framework into consideration, this paper presents an analysis of the energy sharing coefficients proposed by the newly published Portuguese legislation via the assessment of a renewable energy community, formed by public buildings, whose operation varies according to different sharing coefficient applied. Results show that time-variable energy sharing coefficients are the best option to the considered renewable energy community. Collected results also show that larger consumers can extract higher benefits from being integrated on a renewable energy community. These benefits decrease when buildings are allowed to self-consume local generated energy prior to the sharing process as demand inequalities become less important for the computation of the considered sharing coefficients. The entire community also presents better performance in this case.

Hasty decision making and belief inflexibility in the more delusion prone? A modified disambiguating-scenarios paradigm assessing cognitive biases implicated in delusions.

Contemporary models of psychosis imply that cognitive biases such as the jumping to conclusions (JTC), the bias against disconfirmatory evidence (BADE), and the liberal acceptance (LA) bias play a role in the pathogenesis of delusions. Most of the studies investigating the role of cognitive biases, however, have been conducted with socially neutral or abstract stimuli and have assessed patients with established psychoses. For the present study, we aimed to concurrently investigate multiple biases (i.e., the JTC, BADE, and LA biases) in a community sample with a new paradigm using more socially engaging stimuli. A large sample of participants (N=874) recruited via Amazon Mechanical Turk was subdivided into two groups based on the frequency of their psychotic-like experiences (PLEs) according to the positive subscale score of the Community Assessment of Psychic Experiences (CAPE) and matched based on major demographics variables, resulting in two equally sized groups called High-PLE (at least 2 SD above the mean) and Low-PLE (maximum 0.5 above the mean; n=46 for each group). Using a modified version of the written-scenarios BADE task, which emphasized social interactions between agents embedded in the scenario, participants rated the plausibility of response options in the face of new information. In line with previous findings, the High-PLE group demonstrated the JTC, BADE, and LA biases. That is, the members of this group made more decisions after the initial piece of information, were less likely to revise their beliefs in light of new information, and provided higher plausibility ratings for implausible response options compared to the Low-PLE group. Results corroborate prior findings suggesting that the JTC, BADE, and LA biases may be contributing factors in delusional ideation and that metacognitive biases extend to social situations.

종교 갈등의 맥락에서 <쌀 나오는 바위> 설화의 이해

In this study, we believe that the memory of religious conflicts during the religious transition from Ancient times to the Middle Ages is preserved in the story of the tale of <The Rock with Rice>, and we aim to understand the story in the context of religious conflicts and discuss the legend's genre achievements. In the tale of <The Rock with Rice>, the rock was originally sacred, but is now depicted in a state of decline. The monk is depicted as taking up residence in the rock and coveting the goods it offers. Thus, we can see that the rock is good and the monk is evil. However, rather than goodness being encouraged and evil punished, this story shows that goodness is discouraged, and evil is not punished for its misdeeds. The fact that the rock is mentioned as an evidence in the part that talks about evidence, and the monk is not in the text of the narrative, suggests that the narrative community's assessment is favorable to the rock. If we compare this to the historical reality of Buddhist adoption, we can see it as a narrative representation of the gradual decline of conventional beliefs, represented by the rock, as Buddhism, represented by the monk, takes its place and gradually absorbs the territory of conventional beliefs. Compared to tales that negatively characterize conventional beliefs, the tale of <The Rock with Rice> share a narrative arc of Buddhist victory, revealing historical reality without narrative distortion. Comparing the tale of <The Rock with Rice> to those that negatively characterize Buddhism, the shared placement of good and evil figureheads reveals the narrative community's compassion for indigenous deities. As such, the story of the tale of <The Rock with Rice> is unique in that it captures both the historical reality of the conversion to Buddhism and the narrative truth of compassion for the existing deity. This is why the rocks as evidence in this story testify to the historical reality of the decline of conventional beliefs, but also to the overlapping interests of the narrative community.

Internet addiction and suicidal ideation among Chinese college students: the mediating role of psychotic-like experiences.

Individuals with Internet addiction (IA) are at significant risk of suicide-related behaviors. This study aimed to investigate the relationships among IA, psychotic-like experiences (PLEs), and suicidal ideation (SI) among college students. A total of 5,366 college students (34.4% male, mean age 20.02 years) were assessed using the self-compiled sociodemographic questionnaires, Revised Chinese Internet Addiction Scale (CIAS-R), 15-item Positive subscale of the Community Assessment of Psychic Experiences (CAPE-P15), Self-rating Idea of Suicide Scale (SIOSS), and 2-item Patient Health Questionnaire (PHQ-2). The prevalence of IA and SI were 9.3 and 12.1% among Chinese college students, respectively. There were direct effects of IA and PLEs on SI. The total effect of IA on SI was 0.18 (p < 0.001). PLEs mediated the relationship between IA and SI (Indirect effect = 0.07). IA had both direct and indirect effects on SI. These findings enable us to elucidate the mechanism of how IA influences individual SI, which can provide vital information for developing and implementing targeted interventions and strategies to alleviate SI among Chinese college students.

Unravelling urban governance challenges: Objective assessment and expert insights on livability in Longgang District, Shenzhen

With the advancement of urbanization, the assessment of urban livability has garnered widespread attention due to its importance for sustainable urban development. In the data era, the availability of geospatial big data has facilitated the objective livability assessments. However, a purely objective assessment fails to reveal the contradictions inherent in the urban governance or aid policymakers in understanding the challenges they face. Therefore, this study selected Longgang District in Shenzhen, a district with ambitions for livability, as a pilot area for assessment in a complementary way. Firstly, by drawing upon previous indicators, this research collected data from multiple sources, and conducted a comprehensive assessment of communities in Longgang, considering aspects of safety, health, convenience, and comfort. It was found that while most communities achieved high scores in livability, certain areas exhibited poor performance in terms of flooding, pollution, and public facilities, showing significant spatial variations. Generally, there is a hierarchical spatial differentiation in livability, gradually decreasing from the center to the outer areas. To uncover the urban governance challenges behind these spatial findings, thematic analysis was conducted, and semi-structured interviews were carried out with 10 local experts. Four challenges were specifically identified: the lack of supportive policies in flooding governance, difficulty in monitoring pollution, the absence of long-term planning in public facility governance, and disputes over the governance of low livability areas known as 'urban villages'. This study provides an exploratory research approach for assessing livability, and offers insights for future urban governance.

Evaluation of reliability and validity of the Persian version of Peters et al. delusions inventory (PDI-40) in iranian non-clinical and clinical samples

BackgroundSome individuals may manifest psychotic symptoms that do not fulfill the requisite clinical criteria for a formal diagnosis of psychosis. The assessment of susceptibility to delusions, encompassing both clinical and non-clinical cohorts, frequently makes use of the Peters et al. Delusions Inventory (PDI-40). This study aimed to evaluate the reliability and validity of the Persian version of Peters et al. Delusions Inventory (PDI-40) in Iranian non-clinical and clinical samples.MethodsThe present study employed a cross-sectional, correlational design in 2020. A total of 1402 Iranian participants were recruited for the study, which consisted of three distinct stages. The first stage involved an Exploratory Factor Analysis (EFA) conducted on a non-clinical sample of 512 participants. The second stage comprising different non-clinical sample 764 participants to perform a Confirmatory Factor Analysis (CFA). In the third stage, a clinical sample of 126 psychotic patients was compared to a non-clinical sample. All participants completed the PDI-40, the Community Assessment of Psychotic Experiences (CAPE-42), and the Depression, Anxiety, and Stress Scale (DASS-21). The internal structure of PDI-40 was examined through the analysis of its factor structure using LISREL 8.8.ResultsThe EFA analysis unveiled nine components within Persian version of PDI-40. The CFA analysis demonstrated an excellent fit of the nine-factor structure of Persian PDI-40 to the data. The total score exhibited high internal reliability, as indicated by Cronbach’s alpha coefficient of 0.92. Moreover, Persian PDI-40 exhibited satisfactory evidence of convergent validity, as significant correlations were observed between dimensions of PDI-40 and subscales of CAPE-42 and DASS-21. Lastly, findings indicated that psychotic participants scored higher than non-clinical participants in all components of the PDI-40(p < 0.05).ConclusionPersian version of the PDI-40 demonstrates strong reliability and validity for assessing delusion proneness in both non-clinical and clinical samples in Iran. The observed distinctions between psychotic and non-clinical participants underscore its potential as a valuable tool for discerning delusion proneness in diverse contexts.

Association between host nitrogen absorption and root-associated microbial community in field-grown wheat.

Plant roots and rhizosphere soils assemble diverse microbial communities, and these root-associated microbiomes profoundly influence host development. Modern wheat has given rise to numerous cultivars for its wide range of ecological adaptations and commercial uses. Variations in nitrogen uptake by different wheat cultivars are widely observed in production practices. However, little is known about the composition and structure of the root-associated microbiota in different wheat cultivars, and it is not sure whether root-associated microbial communities are relevant in host nitrogen absorption. Therefore, there is an urgent need for systematic assessment of root-associated microbial communities and their association with host nitrogen absorption in field-grown wheat. Here, we investigated the root-associated microbial community composition, structure, and keystone taxa in wheat cultivars with different nitrogen absorption characteristics at different stages and their relationships with edaphic variables and host nitrogen uptake. Our results indicated that cultivar nitrogen absorption characteristics strongly interacted with bacterial and archaeal communities in the roots and edaphic physicochemical factors. The impact of host cultivar identity, developmental stage, and spatial niche on bacterial and archaeal community structure and network complexity increased progressively from rhizosphere soils to roots. The root microbial community had a significant direct effect on plant nitrogen absorption, while plant nitrogen absorption and soil temperature also significantly influenced root microbial community structure. The cultivar with higher nitrogen absorption at the jointing stage tended to cooperate with root microbial community to facilitate their own nitrogen absorption. Our work provides important information for further wheat microbiome manipulation to influence host nitrogen absorption. KEY POINTS: • Wheat cultivar and developmental stage affected microbiome structure and network. • The root microbial community strongly interacted with plant nitrogen absorption. • High nitrogen absorption cultivar tended to cooperate with root microbiome.