Assessment Of Biochemical Changes Research Articles

Assessment of Biochemical Changes among Egyptian Women with Increased Body Weight

Purpose: The aim of this work is to evaluate the relationships between ghrelin and leptin with the metabolic state of overweight and obese participants among Egyptian women. Methods: This study was conducted on 82 participants, their age ranged from 43 to 65 free from cancer or endocrine-related disease (e.g., diabetes) they were divided into three groups according to their body mass index (BMI). Group 1 (G1) with BMI less than 25 Kg/m 2 , Group 2 (G2) with BMI between 25-30 kg/m 2 and Group 3 (G3) with BMI more than 30 Kg/m2. Ghrelin and leptin were determined by ELISA technique, Insulin resistance was measured by homeostasis model assessment and lipid profile was determined in all studied groups. Results: Fasting plasma levels of ghrelin were lower in overweight and obese groups compared to normal weight control group; also, there was statistically significant negative correlation of ghrelin levels with leptin, BMI and HOMA. Our results showed that higher concentrations of fasting leptin were found in overweight and obese groups compared with normal weight control group; also, there was statistically significant positive correlation between leptin and other biochemical parameters; insulin, BMI and HOMA. Conclusion: Our results suggest coordinated roles of ghrelin and leptin in the modulation of the obesity and these markers can be of value in assessment of treatment of such cases.

Interrupting the mechanisms of brain injury in a model of maple syrup urine disease encephalopathy

Maple syrup urine disease (MSUD) was first recognized as an inherited lethal encephalopathy beginning in the first week of life and associated with an unusual odor in the urine of affected children. It was later confirmed as a deficiency of branched-chain keto acid dehydrogenase (BCKDH), which is the second step in branched-chain amino acid (BCAA) breakdown. MSUD is characterized by BCAA and branched-chain keto acid (BCKA) accumulation. BCAAs are essential amino acids and powerful metabolic signals with severe consequences of both deprivation and accumulation. Treatment requires life-long dietary restriction and monitoring of BCAAs. However, despite excellent compliance, children commonly suffer metabolic decompensation during intercurrent illness resulting in life-threatening cerebral edema and dysmyelination. The mechanisms underlying brain injury have been poorly understood. Recent studies using newly developed mouse models of both classic and intermediate MSUD have yielded insight into the consequences of rapid BCAA accumulation. Additionally, these models have been used to test preliminary treatments aimed at competing with blood-brain barrier transport of BCAA using norleucine. Assessment of biochemical changes with and without treatment suggests different roles for BCAA and BCKA in the mechanism of brain injury.

Feasibility of Texture Analysis for the Assessment of Biochemical Changes in Meniscal Tissue on T1 Maps Calculated From Delayed Gadolinium-Enhanced Magnetic Resonance Imaging of Cartilage Data

To (1) establish the feasibility of texture analysis for the in vivo assessment of biochemical changes in meniscal tissue on delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC), and (2) compare textural with conventional T1 relaxation time measurements calculated from dGEMRIC data ("T1(Gd) relaxation times"). We enrolled 10 asymptomatic volunteers (7 men and 3 women; mean age, 27.2 +/- 4.5 years), without a history of meniscus damage, in our study. MRI of the right knee was performed at 3.0 T. An isotropic, 3-dimensional (3D), double-echo steady-state sequences was used for morphologic evaluation, and a dual flip angle 3D gradient echo sequence was used for T1(Gd) mapping. All MRI scans were performed 90 minutes after injection of 0.2 mmol/kg of Gd-diethylenetriamine pentaacetic acid (DTPA), and subsequently, during application of a compressive force (50% of the body weight) in the axial direction. Regions of interest, covering the central portions of the posterior horn of the medial meniscus, were defined on 3 adjacent sagittal sections. Based on the relaxation time maps, mean T1(Gd), as well as the T1(Gd) texture features derived from the co-occurrence matrix (COC: Angular Second Moment, Entropy, Inverse Difference Moment) and wavelet transform (WAV: WavEnLL, WavEnHL, WavEnHH, WavEnLH), were calculated. Paired t tests were used to assess differences between baseline and compression, and intraclass correlation coefficients (ICC) were calculated to establish the intrarater reliability of the measurements. Mean T1(Gd) (-67.3 ms, P = 0.011), Angular Second Moment (-0.0002, P = 0.009), Entropy (+0.033, P = 0.025), WavEnLL (+1011.16, P = 0.002), WavEnHL (+18.64, P = 0.012), and WavEnLH (+72.74, P = 0.035) differed significantly between baseline and compression. Intrarater reliability was substantial for mean T1(Gd) relaxation times (ICC = 0.99-1.0), and also for T1(Gd) co-occurrence matrix (ICC = 0.63-0.92) and WAV (ICC = 0.86-0.98) features. Texture features extracted from T1 maps calculated from dGEMRIC data are feasible for the in vivo assessment of biochemical changes in the menisci, such as might be induced by mechanical loading. Thus, T1(Gd) texture features complement conventional relaxation time measurements. Further studies are necessary to determine whether the mechanical compression, or a prolonged Gd-DTPA uptake, or both, are responsible for the observed decrease in mean T1(Gd) relaxation times in the menisci.

Anti-Inflammatory and Anti-Apoptotic Effects of Fumonisin B1, an Inhibitor of Ceramide Synthase, in a Rodent Model of Splanchnic Ischemia and Reperfusion Injury

Ceramide is a sphingolipid with potent proinflammatory and proapoptotic properties. This study sought to determine whether pharmacological inhibition of ceramide biosynthesis in the intestine attenuates pathophysiological sequelae of shock induced by splanchnic artery occlusion and reperfusion. Ischemia and reperfusion injury was induced in anesthetized rats by clamping both the superior mesenteric artery and the celiac artery for 45 min followed by reperfusion. Within 6 min after reperfusion, animals developed significant systemic hypotension with 100% of the animals dying during the 4-h period of reperfusion. In parallel experiments, animals were necropsied after 60 min of reperfusion, and the ileum was harvested for histological examination and assessment of biochemical changes. Administration of fumonisin B1 (FB1), a competitive and reversible inhibitor of ceramide synthase (3 mg/kg, 15 min before reperfusion), significantly reduced i) the increased ceramide expression as detected by immunohistochemistry; ii) peroxynitrite-mediated protein nitration; iii) infiltration of the reperfused intestine with polymorphonuclear neutrophils following a decrease in intercellular adhesion molecule-1 expression; iv) production of the proinflammatory cytokine tumor necrosis factor-alpha; and v) apoptosis in the ileum. Overall, tissue-protective effects were clearly observed upon histological examination of the ileum. These beneficial events were ultimately linked to decreases in both the development of hypotension and overall mortality. These results implicate ceramide as a key signaling molecule in splanchnic arterial ischemia and reperfusion-induced shock. The broader implications of our results provide a pharmacological rationale for the development of inhibitors of ceramide biosynthesis as novel therapeutics for ischemia and reperfusion-induced shock of several etiologies.

The pathology of fatal child abuse

It is a sad indictment of human society that the abuse of children is such a prevalent and widespread problem. The acknowledgement that physical, emotional and sexual injury as well as intentional neglect can be inflicted upon the young by any person but especially by caregivers has been increasingly realised by the community. As a result, many professionals, especially in the medical sciences, are involved in the study and management of such cases with the ultimate goals of recognising children at risk, diagnosing those cases that have occurred, preventing initial or subsequent injury and bringing perpetrators to justice. The aim of this paper is to review recent published work on the pathology of abuse leading to death of the child. Particular reference is made to the patterns of observed physical damage as well as to the interpretation of those observations. Clearly many more children are abused than die directly as a result of that abuse, but pathologists are infrequently involved in the management of clinical abuse cases. Exceptions to this rule, of course, include assessment of biochemical changes in cases of Munchausen syndrome by proxy, diagnosis of infective lesions resulting from sexual assault as well as the interpretation of unexplained cutaneous lesions subsequently shown to be caused by physical assault (such as burns and bite marks). Cases of physical abuse are usually managed by pediatric specialists with assistance from radiologists, neurosurgeons and ophthalmologists, and it is important that effective communication be maintained by pathologists with these practitioners when investigating a case that has unfortunately culminated in death.

Application of a dehydrated test strip, HEMASTIX®, for the assessment of gingivitis

In order to develop a rapid and simple method based on chemical alterations in gingival pockets during inflammation, the HEMASTIX strips were used to record the degree of gingivitis. This dehydrated test strip records the presence of both hemoglobin derived from erythrocytes and myeloperoxidase from PMN-leukocytes. The intensity of the blue color that appeared and the amount of adsorbed fluid were recorded. The correlation of the Strip Index with Gingival Index was highly significant (P is less than 0.001) and with Gingival Bleeding Index significant (P is less than 0.01). When determined both from the left side of the upper jaw and from the Ramfjord teeth, the correlation between these two areas was highly significant (P is less than 0.001) in the case of Strip Index but only slightly significant (P is less than 0.05) with Gingival Index. According to the present results the Strip Index can be used for the assessment of biochemical changes in gingival pockets resulting from the development of gingivitis.