Assessment Of Bacteriological Quality Research Articles

Assessment of bacteriological quality of ready to eat food (Meat pie) in Benin City metropolis, Nigeria

Eight triplicate samples of meat pie were randomly sampled from standard eatery and local kiosk in Benin City and analyzed microbiologically for the rates ofStaphylococcus aureus and Escherichia coli. The mean microbial load on the fresh meat pie from the standard eatery ranged from 3x103 – 5x103cfu/g while the air preserved and refrigerated meat pie for (2 days) ranged between 2.3 x104 -3.8 x104 cfu/g and 8x 103-1.5 x104 cfu/g respectively. The mean microbial load of the fresh meat pie from the local kiosk ranged between 7x103-2.8x104 cfu/g while the air preserved and refrigerated meat pie for 2days ranged between 3x10-4 to too numerous to count (TNTC) and 1.3 x104 -2.8x104 cfu/g respectively. Six genera of the isolated bacteria include Staphylococcus, E. coli, klebsiella, Pseudomonas, Bacillus and Enterococcus. Statistical analysis of the mean microbial load showed a significant difference (P 0.05). Key words: Escherichia coli, meat pie, mean microbial load, Staphylococcus.

Bacteriological quality and risk assessment of the imported and domestic bottled mineral water sold in Fiji

Considering the popularity of bottled mineral water among indigenous Fijians and tourists alike, a study was carried out to determine the bacteriological quality of different bottled waters. A risk assessment was also carried out. Seventy-five samples of bottled mineral water belonging to three domestic brands and 25 samples of one imported brand were analysed for heterotrophic plate count (HPC) bacteria and faecal coliforms. HPC counts were determined at 22 degrees C and 37 degrees C using R2A medium and a membrane filtration technique was used to determine the faecal coliform (FC) load in 100 ml of water on mFC agar. Between 28 and 68% of the samples of the various domestic brands failed to meet the WHO standard of 100 colony forming units (cfu) per 100 ml at 22 degrees C and 7% of these also tested positive for faecal coliforms. All imported bottled mineral water samples were within WHO standards. A risk assessment of the HPC bacteria was carried out in terms of beta haemolytic activity and antibiotic resistance. More than 50% of the isolates showed beta haemolytic activity and were multi-drug resistant. While the overall quality of the product was generally good, there is a need to enforce stringent quality standards for the domestic bottlers to ensure the safety of consumers.

Use of geographic information system in the assessment of bacteriological quality and sanitary risk factors of household drinking water sources in Ibadan, Nigeria

This study used a combination of Geographic Information System (GIS), bacteriological analysis and sanitary inspections to assess the risk of contamination of drinking water sources in 62 randomly selected households in Ibadan, Nigeria. Findings revealed that the majority of the households used groundwater sources of poor quality. The raw bacteriological data was classified into five water quality Grades A to E (from no risk to very high risk). Majority (82.3%) of the households were grouped as D and E, implying that a large proportion of the households was exposed to high bacterial load in their water supplies thus exposing these households to the risk of water-borne diseases. Results of sanitary inspection also showed that most (62.9%) households have intermediate/high risks associated with physical defects in the water supply facilities which could lead to quality deterioration. A weak positive correlation (Spearman's r =0.379, p =0.02) was observed between the E. coli and sanitary risk score grades. The study proposed urgent remedial action by all stakeholders and an extension of the study to cover the rural and urban local government areas in Ibadan.

Assessment of Bacteriological Quality of Drinking Water in some Household Water Filter Systems in Benghazi City

Assessment of Bacteriological Quality of Drinking Water in some Household Water Filter Systems in Benghazi City

Assessment of Bacteriological Quality of Egyptian Bottled Water

Assessment of Bacteriological Quality of Egyptian Bottled Water

Refrigerated autosampling for the assessment of bacteriological water quality

This study compared manual and automatic sampling techniques at two urban creeks of varying faecal impact in Sydney, Australia. Changes in refrigerated (3°C) automatic sampler samples were followed for up to 18 h for total coliforms, faecal coliforms, total heterotrophs, pH, conductivity and turbidity. Significant differences ( P = 0.000) in faecal coliform densities between manual and automatic samples 0.101 log 10cfu/100 ml were detected at the less impacted site. Also, total coliform levels decreased and then increased during refrigerated storage at the less impacted site. Total heterotrophs grown at 20 or 35°C were not significantly ( P > 0.05) affected by either the method of sampling or storage time. At both sites, the pH of manual samples was consistently lower than that of automatic samples and could not be accounted for by bottle type. In contrast, the higher conductivity of manually collected samples could largely be accounted for by the use of glass rather than plastic bottles. Conductivity at both sites decreased with storage time in plastic bottles. These results signify an inherent difference between samples collected manually and those collected automatically, but indicate that these differences are minimal in practical terms, particularly when water samples are polluted. Sampling technique and storage time appeared to be more critical when the level of contamination to be detected was low.

Observations on the introduction of the DEFT for the routine assessment of bacteriological quality

In the light of experiences gained and observations made in the dairy industry, the critical control points and factors that affect the operation of the DEFT (Direct Epifluorescent Filter Technique) in a routine laboratory were determined. Recommendations are made for the optimization of both the test protocol and the instrumentation, and the need for adequate operator training is strongly emphasized.

External quality assessment data analysis by microcomputer

Microcomputers are now widely used in clinical laboratories because of their low price and the availability of a wide variety of software that can be adapted to the users' requirements. The continuously increasing numbers of patients, tests and the use of automation are additional factors which emphasize the need for computers. A microcomputer software that meets the exact requirements of the Quality Control Unit was developed to evaluate laboratories participating in the Kuwait National Bacteriological Quality Assessment Scheme.

Assessment of Bacteriological Quality of Fast Foods and Soft Drinks in Relation to Safety and Hygiene

Bacteriological quality assessment of fast foods and soft drinks of different establishments of Dhaka City was carried out. For systematic study of varieties of such foods consumed by different class of people, the food samples were divided into four major categories: Type A (upper class), Type B (middle class), Type C (moderate class) and Type D (lower class). The quality of samples was assessed by determining standard plate count (SPC). The SPC showed that about 15% of Type A, 23% of Type B, 43% of Type C and 57% of Type D samples were unsafe for human consumption. Coliform bacteria were detected in about 13-48% of various food varieties. Psychrophilic bacteria were present in about 19-42% samples, indicating not suitable for longterm preservation in refrigeration condition. One sample of Type A and two samples each of Type B, Type C and Type D yielded growth of Salmonella and a considerable number of the samples contained Escherichia coli. In this study it was found that the condition of most of the Type C and Type D food and drinks were unsafe for consumption. The safely and hygienic status of Type A and Type B were comparatively better than those of Type C and Type D. Keywords: Bacteriological quality; Fast food; Soft drink; Safety; HygieneDOI: http://dx.doi.org/10.3329/bjm.v25i1.4863 Bangladesh J Microbiol, Volume 25, Number 1, June 2008, pp 73-75

Bacteriological aspects of pollution in the Jukskei — Crocodile river system in the Transvaal, South Africa

A combination of routine methods of bacteriological analysis has been utilised to investigate conditions in a river system which in part is subject to the effects of the discharge of appreciable quantities of sewage effluents which, during the dry season, may constitute the major part of the total flow. Other parts of the river system are free from gross pollution, and results obtained from these sections served as a basis for comparison. The river system is capable of coping with most of the detrimental effects of direct faecal pollution in varying form. But under no circumstances do these findings imply that the river system is capable of dealing with unrestricted quantities of sewage effluents for an unlimited period of time. Industrial pollution of varying character has been found to have little, if any, effect on bacterial numbers. The effect these effluents may have on the composition of the bacterial population as a whole has not been investigated, but this influence would be of local significance only. The methods used and the interpretations applied to the results seem to constitute a valuable instrument in the detailed study and assessment of bacteriological quality of the waters in a natural river system which is subject to severe pollution from sewage and industrial effluents of varying character.