Human immunodeficiency virus is grouped into the genus Lentivirus within the family of retroviridean, a subfamily of retrovirinae. Since its emergence, it has remained a global public health crisis with approximately 39.0 million (33.1 – 45.7 mil) people living with HIV globally in 2022, with some countries reporting increased trends in new infections and it is incurable. There has been documented success in decreasing new cases and mortality resulting from HIV/AIDS globally; however, in sub-Saharan Africa, the rate of new infections has increased disproportionately among young women. Thus, the region accounts for about 9 – 10 women and children living with HIV. Mother-to-child transmission (MTCT) of HIV occurs when HIV is transmitted from the mother to the child during pregnancy, childbirth, or breastfeeding, although the rate of MTCT of HIV in high-income countries has significantly reduced to less than 1% in some low-income countries, MTCT of HIV remains high at 8.9%, and about 90% of HIV infection in children occurs through MTCT of HIV. Prevention of Mother-to-child transmission (PMTCT) of HIV is recommended to curb the menace of MTCT of HIV (Falnes et al., 2010). It involves a series of events, services, or interventions, which, when fully implemented, result in a reduction in the risk of mother-to-child transmission (MTCT) of HIV (Centers for Disease Control and Prevention, 2016) that involves the use of drugs and non-drug-related services. Health care workers play an important role in the provision of services and interventions aimed at reducing HIV transmission. Therefore, this study intends to Asses Health Care Workers' Attitude Towards PMTCT of HIV At Primary Health Care Center. The study is an institutional-based cross-sectional descriptive survey study. The sample size was calculated using the formula of Yamane (1967), a multistage sampling was used to select sampling unit. A self-administered closed-ended questionnaire was used as the instrument for data collection, all responses to the completed questionnaire were coded and entered into SPSS v25.0, edited, and checked accordingly. Descriptive statistics were used to describe the frequency distribution and measures of central tendency, whereas inferential statistics were used to determine the relationship between the study variables. The study finding reveals that the general attitude score of the respondents on PMTCT of HIV was Positive (3.1) and there is a statistically significant level of association between years of work and attitude (p<0.024). In this study, it can be concluded that the level of health care workers attitude towards PMTCT of HIV was positive and there is a statistically significant level of association between years of work and attitude. This shows that health care workers with more year of working experience have a better attitude, this could be related to the fact that they have more contact time and training on PMTCT and HIV in general, it is recommended that further studies be conducted to the explore the reason behind the statistical association above.