This study focuses on the isolation and characterization of microbes from landfill soil in Gorakhpur, India. Landfills are a major source of soil pollution, and understanding the microbial composition in these environments is crucial for assessing soil quality and developing remediation strategies. Microorganisms can degrade numerous of organic pollutants owing to their metabolic machinery and to their capacity to adapt to inhospitable environment. Thus, microorganisms are major players in site remediation. However, their efficiency depends on many factors, including the chemical nature and the concentration of pollutants, their availability to microorganisms, and the physio-chemical characteristics of the environment. The research identified a diverse microbial community in the landfill soil, with predominant bacterial representatives including Gamma-proteobacteria, firmicutes, and bacteroids. Gram staining revealed the prevalence of gram-positive bacilli, along with distinct fungal species. These findings emphasize the potential of microorganisms in degrading organic pollutants and transforming various compounds in landfill soil. By elucidating the microbial diversity in landfill sites, this study provides insights for sustainable waste management practices and environmental conservation efforts. One of the major cause of soil pollution are landfills. There are the sites designated for dumping rubbish, garbage, or other sorts of solid wastes. Because most of these waste materials are non-biodegradable, they heap in the landfills where they stay for years, impacting on soil quality and polluting the land. The aim of this study is to isolate and investigate the role of microorganisms in a particular landfill area of Gorakhpur, Uttar Pradesh and to identify the microbial community found in that particular area.