Aspergillus Niger Biomass Research Articles

Sorption of Pt(II) on inactive biomass of Aspergillus Niger O-5 treated with cetyltrimethylammonium bromide

Platinum-group cytostatic have been found in natural waters due to the current inefficiency of wastewaters treatment plants. This study was aimed to the assessment of inactive biomass of Aspergillus niger O-5 treated with cetyltrimethylammonium bromide as sorbent for solid phase extraction of [PtCl4]2-complex from aqueous dissolutions for the subsequent development of new water treatment technologies. Maximum sorption capacity of 54 mg·g−1 was achieved at 25 °C under the following experimental conditions: pH of dissolution = 5, biomass mass = 10 mg, dissolution volume = 10 mL, Pt(II) mass = 0.5 mg and contact time = 30 min. The good fitting of the model of Ho and the increase of sorption capacity up to 102 mg·g−1 with temperature suggested the chemisorption as predominant mechanism. The mean free energy of adsorption was higher than 8 kJ·mol−1 for all temperatures, which also indicated the chemical character of the interaction. The absolute value of the standard molar Gibbs free energy of formation increased from 3.032 to 8.336 kJ· mol−1 with temperature signifying that the sorption was thermodynamically favored, occurred spontaneously and the degree of spontaneity increased with temperature. The positive value (32.3 kJ·mol−1) of the standard molar enthalpy qualified the sorption as an endothermic process, after which the adsorbent/sorbate system became more thermodynamically stable.

Investigation of Physicochemical Characteristics of Aspergillus niger Biomass and Examination of Its Ability to Separate Butyl Acetate Isomers.

Aspergillus niger is a species of fungus that is widely found in natural ecosystems and has an important role in various industrial fields and is readily available. To study the adhesion of microbial cells to solid substrates and to improve their properties, physicochemical characterization of microorganisms is extremely important. For this purpose, in this study, the surface properties of A. niger biomass were determined at low cost and with high accuracy by inverse gas chromatography (IGC), a physicochemical characterization technique. IGC experiments were conducted between 303.2 and 328.2K at infinite dilution. Among these temperatures, various organic solvent vapors were passed over the A. niger biomass considered as stationary phase and their retention behavior was studied. Using the raw data, net retention volumes were calculated and retention diagrams were drawn. From the linear retention diagrams, the dispersive surface energy was calculated according to Dorris-Gray (48.73-46.09 mJ/m2), Donnet-Park (47.12-44.50 mJ/m2), Schultz (46.88-42.45 mJ/m2), and Hamieh (76.42-64.06 mJ/m2) methods. With the IGC method, the acidity-basicity parameters of A. niger biomass were determined and it was found that the surface was basic ( ). In the second part of this study, the butyl acetate isomer series, which are difficult to be separated by conventional methods, were effectively separated by the IGC method using A. niger stationary phase.

The use of mixed bomass alga-fungi in mutualistic relationship and natural marine-origin carrier in MBBR reactor for an ecological treatment of effluents from fish processing industry in Morocco

The biological treatment in a fluidised biofilm bed bioreactor (MBBR) of fisheries effluent was studied using a mixed biomass of macro-algae and Aspergillus niger as fungal innoculum. The Green macro algae Ulva lactuca (ULva) and the Brown seaweed genus of Laminaria collected from the coast of Mohammedia city in Morocco, was used to ensure better efficiency in reducing pollution in the cases of mixed biomass bioreactor and of a mutualistic relationship favorable between Algae and fungi. For better ecological and economic advantages this study also considers the biofilm colonization media used and also compared between the use of conventional Kaldnes K3 and sardine scales as a support for biofilm colonization in MBBR bioreactors. The results obtained showed that the use of co-culture of fungi and macro-algae represents an excellent development prospect. The organic substance reduction efficiency obtained in this study, using the mixed biomass between the brown macro alga laminaria and the Aspergillus niger fungi, is better than that obtained by using the biomass containing the green macro alga, especially for the reduction of total nitrogen, so that the abatement rate is up to 78% for the natural sardine shell support and 72% for the synthetic K3 support.

Scale-up of citric acid production by utilizing rice husk waste and purification using ion exchange chromatography

Citric acid is one of the essential organic acids in human life and rice husk has the potential to be used as a substrate because it contains about 28.6-43.3% cellulose, and its use, does not compete with human needs. This research aims to increase citric acid production by using rice husk as a substrate, with a working volume of 2000 mL. In this study, it produced citric acid using submerged fermentation and Aspergillus niger at 22.42% w/v rice husk conditions, 10% w/v sucrose, 3% v/v methanol, and 400 rpm agitation. The results showed that the citric acid pH value on day 18 of fermentation was 1.9, and the concentration of citric acid after purification was 162.667 g/L, while the concentration of citric acid before purification was 148.639 g/L. The specific growth rate of Aspergillus niger obtained was 0.0863 cells/day, and the rate of reducing sugar consumption was 0.1283 g/day. The yield of Aspergillus niger biomass produced is 1.1258 cells for each g of reducing sugar, and the yield of citric acid production is 16.8144 g for each g of cell and 18.9301 g for each g of reducing sugar.

Bio sorption Studies on Textile Effluent Using Packed Bed Reactor with Aspergillus Niger Biomass

The disposal of effluent is a major source of pollution in water bodies, textile effluent is a problem of major concern in the locale. Adsorption is the adhesion of atoms, ions, or molecules from a gas, liquid, or dissolved solid to a surface. Treatment for textile effluent involves the usage of chemicals to remove dye which is incompetent in removing the heavy metals and other organic matter in the effluent which remains unprocessed. The biosorption studies using Aspergillus niger biomass showed an overall decolorization of around 85% with a metal adsorption rate of 81% which can be feasibly utilized for the ecofriendly treatment of textile effluents Key Word: Adsorption, Biomass, Decolourisation, Eco-friendly

A comparative study of the ability of the fungus Aspergillus niger on two alternative media, wheat bran and rice husk.

Citric acid is one of the important organic acids in the fields of industry, agriculture, medicine, etc., and it can be obtained in many ways, the best of which is biological methods using microorganisms. The current study aimed to prepare two cheap and environmentally friendly culture media for the purpose of producing citric acid from Aspergillus niger. The two media, one from rice husks and the other from Wheat bran, where a comparison was made between them regarding which is more efficient in supporting the fungus A. niger compared to the standard medium, the best temperature and pH for production were also assessed.
 Eight isolates were obtained, all of which produced acid to varying degrees. No.1 was the highest and best in terms of production 3.1 gm/l, followed by isolate No. 2 with an amount of 2.5 g/l. It was observed that the amount of acid produced increased with the increase of the biomass of the fungus.
 The study showed variable results regarding the production of citric acid from isolates of A. niger, where the wheat bran medium was the best result, where the resulting concentration of acid was 5.1 gm/l, compared to 4.4 g/l for the medium of rice husks.
 The results showed that the best temperature was 25°C, as it gave a concentration of citric acid of 3.4 g/l, while the best pH was 6 where the concentration of citric acid produced It was 3 grams per liter.the study showed that there were no significant differences with regard to the sugars adopted in this experiment, as it gave significant similar results in terms of the amount of acid produced as well as the biomass of the fungus Aspergillus niger, while ammonium nitrate was the best among the nitrate sources used, with a production quantity of 4 gm/l .

Production, nanoformulation, and potential anticancer effect of camptothecin metabolites from biomass of Aspergillus niger

Production, nanoformulation, and potential anticancer effect of camptothecin metabolites from biomass of Aspergillus niger

Biosorption removal of iron from water by Aspergillus niger

The expulsion of iron from water is an essential issue. Exceeding iron concentrations in water, it become more toxic and cause several troubles for human health and environment. The biosorption is the upcoming mechanism to treat the iron from wastewater. Microorganisms perform an important function in the bioremediation of wastewater. This study was conducted to investigate the removal of iron by dried biomass of Aspergillus niger (A.niger). The dried A. niger was tested as a sorbent for the removal of iron from wastewater. The effects of various experimental parameters as initial iron concentration, amount of biomass, contact time and the initial pH solution were examined and optimal experimental conditions were obtained. The obtained adsorption results were fitted to the Langmuir, Freundlich, and Temkin equations. The study showed that dried A. Niger biomass in high concentration 4 g/100 ml was found to be more effective in the removal of iron from water at pH 3 with contact time 60 min. the A. Niger successfully removed iron and has ability to be regenerated and reused in the removal process.

Biosorption studies of methylene blue dye using NaOH-treated Aspergillus niger-filled sodium alginate microbeads

The effectiveness of NaOH-functionalized Aspergillus niger-filled sodium alginate microbeads on methylene blue (MB) removal was investigated in this study. First of all, Aspergillus niger biomass was obtained, then functionalized using sodium hydroxide (NaOH). Lastly, the composites were prepared by dispersing NaOH-treated-Aspergillus niger in sodium alginate gel. The cross-linked composite microbeads in a 3% CaCl2 solution were characterized by Fourier-transform infrared-attenuated total reflection (FTIR-ATR) spectroscopy, atomic force microscope (AFM), and scanning electron microscope (SEM). FTIR-ATR results indicated that the hydroxyl, amine, and carboxyl groups on the surface of the composite beads acted as binding sites. It was determined that the biosorption of MB occurred via the electrostatic interactions, Van der Waals interactions, π-π interactions, and H-bonding between functional groups and dye molecules. SEM and AFM analyses proved the presence of adsorbed dye molecules on the surface. Besides, optimum conditions were determined by examining the effects of different physicochemical parameters on biosorption. The maximum biosorption capacity of microbeads was obtained as 322.6 mg/g in pH = 7 at 25 °C. Moreover, various isotherms were used, including Freundlich, Langmuir, Dubinin-Radushkevich, and Harkins-Jura. The Langmuir model was found to be the best feasible when the correlation coefficients were taken into account. Biosorption followed the pseudo-second-order kinetic and occurred exothermically and spontaneously. This study indicates that NaOH-treated Aspergillus niger-filled sodium alginate microbeads are promising material as a biosorbent.

Dry biomass of Aspergillus niger in commercial diets for juveniles of Nile tilapia (Oreochromis niloticus) in the intermediate growth phase (15 to 70g)

The objective of the present study was to evaluate different inclusion levels of drymass of the yeast Aspergillus niger (0; 0.125; 0.250; 0.500; 1.00; 2.00; 4.00 and 8.00%) in commercial diets for juveniles of Nile tilapia (16.11 ± 1.74g) in a water recirculation system. The productive performance, organosomatic indexes and carcass composition of these animals were evaluated after 58 days of experiment. A total of 192 juveniles were distributed among 32 tanks made up of eight treatments with four repetitions. A significant difference was observed for the hepatosomatic index at 2.00% of inclusion, which was similar to the other treatments except for the 0.125% inclusion level. The inclusion of A. niger had no negative impacts on performance when compared with the control diet. Since it is a by-product, the use of A. niger can be an alternative in the formulation of diets for tilapia in the initial stages (15-70g).

Cell-Free Protein Synthesis with Fungal Lysates for the Rapid Production of Unspecific Peroxygenases.

Unspecific peroxygenases (UPOs, EC 1.11.2.1) are fungal biocatalysts that have attracted considerable interest for application in chemical syntheses due to their ability to selectively incorporate peroxide-oxygen into non-activated hydrocarbons. However, the number of available and characterized UPOs is limited, as it is difficult to produce these enzymes in homologous or hetero-logous expression systems. In the present study, we introduce a third approach for the expression of UPOs: cell-free protein synthesis using lysates from filamentous fungi. Biomass of Neurospora crassa and Aspergillus niger, respectively, was lysed by French press and tested for translational activity with a luciferase reporter enzyme. The upo1 gene from Cyclocybe (Agrocybe) aegerita (encoding the main peroxygenase, AaeUPO) was cell-free expressed with both lysates, reaching activities of up to 105 U L−1 within 24 h (measured with veratryl alcohol as substrate). The cell-free expressed enzyme (cfAaeUPO) was successfully tested in a substrate screening that included prototypical UPO substrates, as well as several pharmaceuticals. The determined activities and catalytic performance were comparable to that of the wild-type enzyme (wtAaeUPO). The results presented here suggest that cell-free expression could become a valuable tool to gain easier access to the immense pool of putative UPO genes and to expand the spectrum of these sought-after biocatalysts.

Fungi fabrication, characterization, and anticancer activity of silver nanoparticles using metals resistant Aspergillus niger

The purpose of this study was to assess the bio-fabrication possibilities of pre-isolated (from bauxite mine tailings) metal-tolerant Aspergillus niger biomass filtrate and the anticancer potential of synthesized silver nanoparticles (AgNPs) tested with a Human Cervical cancer cell line (HeLa cells: Henrietta Lacks cells). The nitrate reduction test demonstrated that A. niger has the ability to reduce nitrate, and filtrate derived from A. niger biomass efficiently fabricated AgNPs from AgNO3, as demonstrated by a visible color change from pale greenish to brownish. The UV–visible spectroscopy analysis revealed an absorbance peak at 435 nm, which corresponded to the AgNPs. These AgNPs have been capped and stabilized with several functional groups related to various bioactive molecules such as aldehyde, benzene rings, aldehydic, amines, alcohols, and carbonyl stretch protein molecules. Fourier-Transform Infrared Spectroscopy (FTIR) analysis confirmed the capping and stabilizing chemical bonding pattern. Scanning Electron Microscopy (SEM) revealed that the synthesized AgNPs were spherical, with an average size of 21.38 nm. This bio-fabricated AgNPs has in-vitro anticancer potential when tested against the HeLa cell line due to its potential size and shape. At 100 g mL−1 concentrations of this bio-fabricated AgNPs, the anticancer activity percentage was found to be 70.2%, and the IC50 value was found to be 66.32 g m−1. These findings demonstrated that the metal-tolerant A. niger cell filtrate could produce AgNPs with anticancer potential.

Optimisation of conditions for deacetylation of chitin-containing raw materials

The paper describes the differences between chitosan and chitin, and reviews works by foreign scientists on obtaining chitosan from various raw materials. Methods of modifying chitosan and obtaining combined sorbents have been analysed. It has been studied whether chitosan is applicable in the technology of wines and alcoholic beverages as a sorbent. The purpose of the study was determining the optimal conditions of the deacetylation stage to obtain chitosan with the best sorption properties from Aspergillus niger biomass. A three-factor experiment has been carried out. It involved obtaining 27 samples of chitosan using sequential four-step acid-base hydrolysis under various conditions of the deacetylation stage. The deacetylation process was optimised under alkaline conditions depending on the alkali concentration, processing temperature, and exposure. For each of the samples obtained, the adsorption activity, specific surface area, and distribution coefficient in the sorbent–sorbate system have been determined. The degrees of deacetylation of all chitosan samples have been determined by potentiometric titration. The study has resulted in determining the optimal conditions for the deacetylation stage: processing temperature 110–130°C, sodium hydroxide concentration 27–36 g/dm3, exposure 45 to 65 minutes. The sample deacetylated at the temperature 120 °C, alkali concentration 30 g/dm3, and exposure 45 minutes has shown the best adsorption activity values: the adsorption activity for methyl orange 347.96 mg/g, the specific surface area of the sorbent samples 0.52·105 m2/g, the distribution coefficient in the sorbent–sorbate system 3.29·10-3 ml/g. This sample had the highest degree of deacetylation, 43.6%. The sample has been analysed using IR spectroscopy, and its main characteristic frequencies have been studied. It has been concluded that the sample obtained was equivalent to the reference chitosan

Enrichment of Therapeutically Significant Flavonolignans of Silybum Marianum in Vegetative Parts by Applying Fungal Elicitors, Methyl Jasmonate and Silver Nanoparticles as Elicitor in Hydroponic Culture

Introduction: Medicinal plants are being used to treat several diseases for many decades and this is an ancient method to treat the patients. Herbal plant Silybum marianum found most effective one to cure liver disorders. This plant produces silymarin which is a secondary metabolite and have hepatoprotective properties. Silymarin is a mixture of flavonolignans (silybin A, silybin B, isosilybin A isosilybin B, silychristin, silydianin apigenin 7-D glucose and taxifolin) that has antiviral, antibacterial, antifungal and antiallergenic properties. Therefore, the excessive production of silymarin is necessary to cure different types liver disorders, so the present study executed to boost up production of Silymarin flavonolignans in vegetative parts of the Silybum marianum plant by using different elicitors.
 Materials and Methods: In the present study, elicitation technique in hydroponics system was used to enhance the production of pharmacologically active flavonolignans in Silybum marianum. Fungal elicitors prepared from lyophilized Aspergillus niger biomass, methyl jasmonate, silver nanoparticles and combination of silver nanoparticles and methyl jasmonate were added (0.2g/l), (100µM/l), (1 ppm) and (100µM/lppm) in hydroponics with Hoagland’s solution in hydroponics to enhance the production of flavonolignans of Silybum marianum. Controls were also set for each treatment. Plants were harvested after 72 hours of introduction of elicitors. High performance liquid chromatography technique was used for analytical purpose. Four solvents (methanol, acetonitrile, chloroform and 2% Trifloro acetic acid) were used in HPLC. Column was C18 and run time of sample was 1 hour. Silybum marianum’s seed extract was used as a standard. Extract of control and treated plants were run on the same polarity in HPLC.
 Results: Results showed that after elicitation significant increase was observed in production of silymarin’s flavonolignans (silybin A, silybin B, isosilybin A, isosilybin B and apigenin 7-D glucose) in vegetative part of the plant but rate of production was different for each elicitor, fungal elicitors prepared from lyophilized Aspergillus niger biomass proved best among all treatments.

Assessment of mycogenic zinc nano-fungicides against pathogenic early blight (Alternaria solani) of potato (Solanum tuberosum L.)

The present investigation was carried out to evaluate the efficacy of mycogenic zinc oxide nanoparticles (ZNONPs) as antifungal agents against Alternaria solani (early blight of potato). The nanoparticles were synthesized biologically by using Aspergillus niger biomass and characterization was done by UV–Vis Spectroscopy, Field Emission Scanning Electron Microscopy (FESEM), Energy Dispersive X-Ray (EDX), Dynamic Light Scattering (DLS), Fourier Transform Infra Red (FTIR), Atomic Force Microscopy (AFM) and inductively coupled plasma mass spectrometry (ICPMS). The UV–Vis spectroscopy showed peaks between the range 250 nm to 340 nm, corresponding to ZNONPs. The FESEM results also confirmed the synthesis of fine sized and spherical ZNONPs. EDX analysis result showed the optical absorption peak corresponding to ZNONPs. The DLS result confirmed the synthesis of ZNONPs with average size of 96.43 nm. FTIR analysis depicted information about all the chemical interactions of ZNONPs. AFM image depicted the three dimensional conformations of ZNONPs. The ZNONPs were tested against A. solani in vitro and minimum inhibitory concentration (MIC) was calculated. Field experiment was conducted for comparison of the efficacy of nanoparticles and chemical fungicide at the parameters of disease severity, tuber number and tuber weight. The data were analyzed by SPSS for analysis of variance (ANOVA) with least significance difference (LSD) and results were found to be significant for different parameters at 0.05 significance levels. It may be concluded by the experiment that ZNONPs may be proved to be potential fungicides in near future and it is an excellent alternative to chemical fungicides.

Removal of textile azo dyes with mixed biomass of Aspergillus niger and orange peel (Citrus sinensis L. Osbeck)

This study evaluates the removal of textile dyes using mixed adsorbents prepared by the growth of Aspergillus niger in orange peels. The highest azo dye removal efficiency was obtained at pH 2, solid: liquid ratio (1: 4 g·mL-1) and time of equilibrium of 250 minutes for each dye. The concentrations of Remazol Black B (RB) and Remazol Red (RR) in both synthetic textile effluents were between 25 mg·L-1 and 100 mg·L-1. The mixed adsorbent was characterized by X-ray diffraction (XRD), spectroscopy infrared region (FTIR) and scanning electron microscopy (SEM). The results indicated that there was a 100% removal of RB and 94.85% of RR at the concentration of 25 mg·L-1. At the concentration of 100 mg·L-1, the percentages of removal reached 98.87% for RB and 96.37% for RR, respectively. The proposed mixed adsorbent was able to remove the textile dyes, presenting adsorptive capacities of 20.77 mg·g-1 and 19.28 mg·g-1 for the dyes RB and RR. Regarding the adsorption kinetics, the experimental data showed that the pseudo second order model was the one that best explained the adsorptive process. For the equilibrium results, the Langmuir model and the Langmuir-Freundlich model were the ones that best fit the experimental data of RB and RR, respectively. The mixed adsorbent produced is a promising alternative for the treatment of textile effluents.

Water hyacinth biochar and Aspergillus niger biomass amalgamation potential in removal of pollutants from polluted lake water

The excess load of organic and inorganic pollutants in Chanramkudi Lake water creates eutrophication resulting from excess growth and spreading of water hyacinth and bad odor and provides favorable circumstances for the survival of disease spreading insect vectors and possibilities of disease occurrence. The results showed that the suitable temperature and residence time for the conversion of dried biomass of water hyacinth into useful biochar was 350 °C and 60 min, respectively, and 33.6% of water hyacinth biochar (WHBC) at stable organic content basis of 49.2% was produced. The WHBC produced under this condition had physicochemical properties such as 389.8 mmol kg−1 of CEC (Cation Exchange Capacity), 69.1 of C/N, 68.2 of C, 234.7 mg kg−1 of total P, 19.7% of ash, and 59.4% of volatiles with pH 7.9. The metal adsorbing competence of this WHBC with and without A. niger was tested with various concentrations (250, 500, 750, and 1000 mg L−1) of various metal (Cd, Cr, and Pb) containing solutions. The WHBC with A. niger amalgamation (15:3 g) effectively adsorbed the metals even at 1000 mg L-1 such as Cr (91.5%), Cd (95.6%), and Pb (93.9%) than individually. The metals were adsorbed and immobilized in the pores of WHBC and the outer layer of the cell wall of A. niger was observed under SEM analysis with various scaling (50, 100, and 150 µm). Further, the pollutant adsorbing functional groups were found in both WHBC and dead mycelia biomass of A. niger through FTIR analysis. The attained results concluded that the biochar produced from challenging water hyacinth could adsorb and minimize the pollutants with an amalgamation of A. niger. Significantly, more volume (33.6%) of biochar can be derived from water hyacinth. Furthermore, WHBC and A. niger are easily cultivable. Thus, it could be preferably useful for removing real-world organic and inorganic pollutants from polluted water.

PERBANDINGAN MODEL ISOTERM BIOSORPSI Cd TERLARUT DALAM AIR DENGAN BIOSORBENT ASPERGILLUS NIGER

Due to the increasing awareness of the deleterious ecological effects of toxic Cd, the adsorption of Cd from aqueous solutions by Aspergillus niger biomass was studied. The aim of this work was to find the exact adsorption isotherm model to predict the single system adsorption by the Aspergilfus niger biomass. The batch sorption experiments were carried out in 50 ml conical flasks. Weighed amounts (8,5 to 57, 2 mg) of Aspergillus niger biomass were introduced into a number of conical flasks, to which 25 ml of Cd solutions of increasing concentrations from 20,28 to 24,435 mg/L were added. The amount of Cd accumulated on biomass was calculated as the difference between the amount present in control experiments and that remaining in solution after equilibrium with biomass. Four adsorption isotherm models, namely Freundlich Isotherm Model, Langmuir Isotherm Model, Sips Isotherm Model, dan Toth Isotherm Model, were fitted to the experimental data. The Toth Isotherm Model and The Sips Isotherm Model· were superior, which provided the least average relative error (6,035 % and 6,013 %). The maximum sorption was seen at pH 6. ltis 143.093 mglg sorbent.

Biosorption of Phenolic Compounds in Lignocellulosic Biomass Biorefineries

The phenolic compounds released during the pretreatment of lignocellulosic biomass negatively affect the saccharification and alcoholic fermentation process. Therefore, the development of strategies to minimize the negative impact of the released inhibitory compounds is key to improve the overall yield and techno-economic viability of future biorefineries. This work proposes the use of Aspergillus niger biomass as a low-cost biosorbent for removal of the phenolics released during liquid hot water (LHW) pretreatment of sugarcane bagasse. Such approach would be advantageous given that fungal microbial biomass could be readily available in biorefineries, as a co-product of the in-house production of cellulolytic enzymes. Three types of A. niger biomass powders were used for adsorption of vanillin (monomer), tannic acid (oligomer), and total phenolics from the LHW pretreatment liquor. A 98% removal of tannic acid was obtained by the biosorbent treated with sodium hydroxide, while adsorption of vanillin was lower, with removal of around 30%. Removal of around 40% was obtained for the total phenolics present in the LHW pretreatment liquor, resulting in a remarkable 50% increase of glucose release in the saccharification of sugarcane bagasse. These findings showed the potential of using the fungal biomass as cost-effective biosorbents for removing the inhibitors released in the pretreatment process and improving the conversion efficiency in biorefineries.

English

Efficiency of biosorbent from chitosan coated dead fungal biomass of Aspergillus niger for dye removal with its impact on metals and physicochemical parameters of textile industry wastewater were assessed. Dye and metal concentrations of wastewater were determined using UV and Atomic Absorption Spectrophotometers respectively, before and after column biosorption using simulation optimized parameters with biosorbent. Physicochemical parameters were determined using standard methods. Simulated and industrial wastewaters data were analysed statistically with one way ANOVA and T-test. Biosorption of dye was classified with Langmuir and Freundlich isotherms. Results showed that at dye concentration of 100 mg/L, 6.90 mg/g of indigo dye adsorbed per mass (qe); in 1.87 mg/L of wastewater, 0.07 mg/g of dye adsorbed per unit mass (qe). Isotherm model fitted well to Freundlich with constant 0.04 and 8.45 mg/g in textile and simulated wastewater respectively. Metals in wastewater ranged from 0.071 to 1.102 mg/L. Acidity, COD Zn, Pb, Fe, Ni, Mn, Cu, Cd, Cr, Co, Mg ions were reduced by 32.94, 39.02, 84.95, 34.57, 44.19, 42.19, 49.09, 25.13, 43.66 and 32.03% respectively after treatment while TDS, conductivity, alkalinity, turbidity, pH increased. Chitosan coated A. niger could be effective in removing dyes and heavy metals from textile wastewater. Key words: Aspergillus niger, biosorbent, physico-chemical, heavy metals, textile wastewater.