Functional Aspects Research Articles

Temporal stability of the rumen microbiome and its longitudinal associations with performance traits in beef cattle

The rumen microbiome is the focus of a growing body of research, mostly based on investigation of rumen fluid samples collected once from each animal. Exploring the temporal stability of rumen microbiome profiles is imperative, as it enables evaluating the reliability of findings obtained through single-timepoint sampling. We explored the temporal stability of rumen microbiomes considering taxonomic and functional aspects across the 7-month growing-finishing phase spanning 6 timepoints. We identified a temporally stable core microbiome, encompassing 515 microbial genera (e.g., Methanobacterium) and 417 microbial KEGG genes (e.g., K00856—adenosine kinase). The temporally stable core microbiome profiles collected from all timepoints were strongly associated with production traits with substantial economic and environmental impact (e.g., average daily gain, daily feed intake, and methane emissions); 515 microbial genera explained 45–83%, and 417 microbial genes explained 44–83% of their phenotypic variation. Microbiome profiles influenced by the bovine genome explained 54–87% of the genetic variation of bovine traits. Overall, our results provide evidence that the temporally stable core microbiome identified can accurately predict host performance traits at phenotypic and genetic level based on a single timepoint sample taken as early as 7 months prior to slaughter.

Formation of Modern Ski Complex

The paper relevance is determined by the growing popularity of active extreme tourism, where ski holidays offer increasing accessibility to ski infrastructure and are supported by the government.Purpose: Identification of design principles and trends in a modern ski complex.Methodology: The analysis of Russian and foreign experience; functional typology of ski complexes; principles of creating modern architecture integrated into the environment; ski resorts in Russia, European countries, Japan and the USA in the 20-21st centuries.Research findings: The historical aspect of the emergence of skiing as a popular tourism is considered herein. Based on the comparative analysis, the principles of designing ski complexes are formulated. The connection between these principles and sustainable architecture is shown. The classification is given to ski complexes. The influence of residence time on functional content is highlighted. The functional planning aspects of design and the influence of architectural and stylistic solutions on them are formulated. The functional planning structure is presented. The dependence of spatial arrangement with the functional planning structure is demonstrated.Practical implications: The results can be used in the design of ski complexes.Originality: The paper studies Russian and foreign experience in design of ski complexes; factors influencing the design in the complex terrain; determines the functional and planning structure and nomenclature of the premises of ski complexes.

Sociopolitical context and COVID-19 fatality rates in OECD countries: a configurational approach

BackgroundThe effectiveness of crisis response can be influenced by various structural, cultural, and functional aspects within a social system. This study uses a configurational approach to identify combinations of sociopolitical conditions that lead to a high case fatality rate (CFR) of COVID-19 in OECD countries.MethodsA Fuzzy set qualitative comparative analysis (QCA) is conducted on a sample of 38 OECD countries. The outcome to be explained is high COVID-19 CFR. The five potentially causal conditions are level of democracy, state capacity, trust in government, health expenditure per capita, and the median age of population. A comprehensive QCA robustness test protocol is applied, which includes sensitivity ranges, fit-oriented robustness, and case-oriented robustness tests.ResultsNone of the causal conditions in both the presence and negation form were found to be necessary for high or low levels of COVID-19 CFR. Two different combinations of sociopolitical conditions were usually sufficient for the occurrence of a high CFR of COVID-19 in OECD countries. Low state capacity and low trust in government are part of both recipes. The entire solution formula covers 84 percent of the outcome. Some countries have been identified as contradictory cases. The explanations for their COVID-19 CFR require more in-depth case studies.ConclusionsFrom a governance perspective, the weakness of government in effectively implementing policies, and the citizens’ lack of confidence in their government, combined with other structural conditions, serve as barriers to mounting an effective response to COVID-19. These findings can support the idea that the effects of social determinants of COVID-19 outcomes are interconnected and reinforcing.

Impact of Central Sensitization on Clinical and Functional Aspects of Psoriatic Arthritis.

Background/Objectives: Psoriatic arthritis (PsA) is an inflammatory rheumatic disease characterized by peripheral arthritis, enthesitis, spondylitis and psoriasis. The objective of this study was to examine the prevalence of central sensitization (CS) and its impact on the clinical and functional aspects of PsA. Methods: Adult patients with PsA according to the Classification of Psoriatic Arthritis (CASPAR) criteria were included in this cross-sectional observational study. The Central Sensitization Inventory (CSI) was used to assess the presence of CS. The study evaluated the impact of CS on individuals by analyzing many factors including demographic information, laboratory findings, clinical features, disease activity, quality of life, severity of sleeplessness, frequency of depression and anxiety. The patients were categorized into distinct groups based on the existence and intensity of CS, and a comparative analysis was conducted on their respective outcomes. Results: A total of 103 PsA patients with a mean age of 43.2 (SD: 6.7) years and including 42 (40.8%) males were included. The mean CSI score was 45.4 (SD: 15.1), and 67 (65.1%) patients had CS. The logistic regression analysis revealed that the variables Psoriasis Area Severity Index (PASI), General Anxiety Disorder-7 (GAD-7), and Insomnia Severity Index (ISI) exhibit considerable predictive power in relation to the outcome variable CS (p < 0.05). PASI was observed as the most important variable in predicting CS (OR 9.70 95% CI: 1.52-62.21). Conclusions: CS has demonstrable efficacy in influencing laboratory, clinical, and functional markers among individuals with PsA. When assessing pain sensitivity in these patients, it is important to take into account the presence of CS.

Medial meniscal posterior root tear – A current concept review

Menisci are crucial structures in the knee joint for the adequate distribution of hoop stresses. Their significance in preventing early knee osteoarthritis has been acknowledged due to the rapid progression of osteoarthritis in knees following meniscectomy. The anatomy of menisci has been studied in detail with an increased understanding of the importance of meniscal root attachments. This review aims to provide an evaluated summary of the anatomical, biochemical, and functional aspects that are relevant in the clinical context of meniscus root attachments, alongside contemporary strategies for accurately diagnosing and treating common injuries affecting these attachments. We also propose an algorithm for managing medial meniscus root tears which may be beneficial for the readers. We did an up-to-date literature search on PubMed, Scopus, and Google Scholar database, using the keywords ‘meniscus’, ‘meniscal root’, ‘sports injury’, and ‘arthroscopy’ in April 2024 and filtered out the relevant literature for this review on the articles that were published in English. The management of root tears requires a high level of suspicion which is crucial for diagnosis, identifying subtle signs on radiology, and employing specific methods for root repair. The tears or avulsions within one centimeter of the tibial attachment of the medial meniscus posterior root are called medial meniscus posterior root tears. These injuries cause a functional meniscal deficiency, resulting in knee problems unfavorable to biomechanics. Numerous methods have been created to tackle meniscus root tears., with many demonstrating promising outcomes in terms of complete healing.

Shortbread cookies reformulation by raspberry powder enrichment: functional and sensory aspects

SummaryThe consumer is looking for foods that can satisfy the need to combine nutrition and well‐being, not only balanced in their composition but also endowed with properties that can improve, or at least maintain, a good state of health. The study aims to enrich the short‐crust pastry with raspberry powder, as a base for breakfast biscuits, increasing its antioxidant properties. Optimising the extent of integration with raspberry powder helped avoid interference with the cross‐linking process of the gluten mesh. The reformulation of shortbread cookies was compared with that of non‐enriched biscuits to define chemical composition, antioxidant potential, and sensory profile. A consumer test helped to evaluate the acceptability of the new formulations. The results indicate that the integration with the optimal identified percentage of raspberry powder, by‐products of juice production, represents an excellent matrix to increase the health properties of shortbread cookies, improving functionality and sensory acceptability.

Development and Usage of Refrigeration and Air Conditioning Trainer for Beginners

This study aimed to evaluate the effectiveness of the developed trainer for refrigeration, which is constructed through the specifications of the users. It focuses on the primary usage, functionality, applicability, and safety aspects of the project, as well as how it effectively assists people through innovative means. The project is tested out of 15 respondents that has knowledge regarding refrigeration and air-conditioning, enough to understand the flow of the project its material used, functions, usage and how it works. Based on the comprehensive evaluation the following key findings have emerged. Functionality Excellence, The prototype trainer demonstrates exceptional functionality, achieving an excellent rating. This indicates a high level of effectiveness in performing various task/activity in refrigeration works. Applicability of the device exhibits versatility with high ratings. Its adaptability to diverse environments underscores its broad applicability.User safety of The trainer dependability is further enhanced by the removal of potentially hazardous materials and the addition of overload protection.

Factors Associated with Self-Reported Health (SRH) among Nepalese Older Men

Background: Self-reported health (SRH) is a subjective measurement tool of overall health status. It integrates with the biological, social, mental, and functional aspects of individuals. The aim of this article is to assess the SRH and its correlates among community-living older men from Nepal. Methods: This study uses the secondary data collected by the Central Department of Population Studies at Tribhuvan University (TU). This study includes community-dwelling male elderly aged 60+ in the Pharping area of Kathmandu. For this study, there were 657 valid responses. A simple and direct question was used “In general, how do you rate your general health?” Its answer was recorded on the 5-point Likert scale “Very Good to Very Bad”. Results: The mean age of the participants was 69.51 years. The majority of respondents (75.9%) live with family members. People who reported poor SRH were 17.0%. Many of them have physical health problems (73.2%) and mental health problems (57.2%). Mental health problems, age of respondents, physical health problems, satisfaction with current living arrangements, walking efficiency, adequate income sources, Insecurity feelings, Income sufficient to fulfill the basic needs, and current working were correlated with SRH. However, linear regression shows that satisfaction with walking efficiency, physical health, and working situation, were significant predictors of SRH. Conclusion: This analysis suggests that working, walking ability, physical health problems, and financial adequacy are significantly associated with self-rated health. Identifying these variables through self-rated status can support the planning of actions aimed at improving the health of the community living older adult men in Nepal.

Diaphragm muscle parameters as a predictive tool for weaning critically ill patients from mechanical ventilation: a systematic review and meta-analysis study.

Diaphragmatic ultrasound, valued for its portability and safety, assesses both structural and functional aspects of the diaphragm. While some studies support its predictive value, others conflict. This meta-analysis aims to clarify diaphragmatic ultrasound's role in predicting successful liberation from mechanical ventilation in intensive care settings. A systematic search was performed on Web of Science, Scopus, and PubMed up to March, 2024. The search strategy included a combination of relevant medical subject heading (MeSH) terms and relevant keywords. We defined our eligibility criteria based on the PICO framework. Two authors performed the data extraction using a standardized sheet. The pooled mean difference was calculated using random effects model and Hedges' g along with SD estimation. R and RStudio were used for the statistical analysis and creating forest and funnel plots. The pooled mean difference was 7.25 (95% CI: 4.20, 10.21) for DE among the two groups. We found a statistically significant difference between the two groups indicating that those with successful weaning from intubation had higher means of DE compared to those with failed weaning attempt (p-value<0.01). The mean difference of DTF was also higher among those with successful weaning from intubation compared to those with failed weaning attempt with the pooled mean difference of 14.52 (95% CI: 10.51, 18.54, p-value<0.01). The mean difference of RSBI was lower among those with successful weaning from intubation compared to those with failed weaning attempt with the pooled mean difference of -28.86 (95% CI: -41.82, -15.91, p-value<0.01). Our results suggest that evaluating diaphragmatic excursion and thickening fraction can reliably anticipate successful liberation from mechanical ventilation. However, significant heterogeneity was present among the included studies. High-quality research, particularly randomized clinical trials, is required to further elucidate the role of diaphragmatic ultrasound in predicting weaning from mechanical ventilation.

Exosomal Non-coding RNA Derived from Mesenchymal Stem Cells (MSCs) in Autoimmune Diseases Progression and Therapy; an Updated Review.

Inflammation and autoimmune diseases (AD) are common outcomes of an overactive immune system. Inflammation occurs due to the immune system reacting to damaging stimuli. Exosomes are being recognized as an advanced therapeutic approach for addressing an overactive immune system, positioning them as a promising option for treating AD. Mesenchymal stem cells (MSCs) release exosomes that have strong immunomodulatory effects, influenced by their cell of origin. MSCs-exosomes, being a cell-free therapy, exhibit less toxicity and provoke a diminished immune response compared to cell-based therapies. Exosomal non-coding RNAs (ncRNA), particularly microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) are intricately linked to various biological and functional aspects of human health. Exosomal ncRNAs can lead to tissue malfunction, aging, and illnesses when they experience tissue-specific alterations as a result of various internal or external problems. In this study, we will examine current trends in exosomal ncRNA researches regarding AD. Then, therapeutic uses of MSCs-exosomal ncRNA will be outlined, with a particle focus on the underlying molecular mechanisms.

Assessing Affective Dimensions of the Interpersonal Circumplex: Development and Validation of the Interpersonal Emotion Inventory

Interpersonal emotions are feelings distinctively associated with real or imagined interpersonal situations. We propose that interpersonal emotions can be organized within the same circumplex used by contemporary integrative interpersonal theory to organize other aspects of interpersonal functioning. Our research explores the affective dimensions underpinning the interpersonal circumplex (IPC) and maps distinct emotional dispositions using coordinates for agentic and communal affect. Our aim is to provide a better differentiated and more comprehensive taxonomy of interpersonal emotions consistent with the IPC framework. In an initial development study (N = 1223 undergraduates) we used circumplex and psychometric criteria to create a 64-item Interpersonal Emotion Inventory (IEI), a novel self-report measure of interpersonal emotions reflecting each IPC octant. We then tested the structural properties and utility of the IEI in a preregistered validation study (N = 278 community participants). Across both studies, the IEI scales exhibited good fit to a two-dimensional circular model. The IEI demonstrated robust convergent, discriminant, and incremental validity in relation to measures of interpersonal values and problems, positive and negative affect, adult attachment, self-conscious emotions, self-esteem, and facets of narcissism. By complementing existing measures of affect and interpersonal phenomena, the IEI should prove useful in both research and clinical contexts.

Aplikasi Material Rotan Pada Perancangan Table Lamp

Rattan is one of the main materials in simple crafts and a material for interior furniture items. In Indonesia, rattan is very abundant and is one of the largest rattan producing countries in the world. On the other hand, this rattan material is very popular with furniture entrepreneurs because rattan can be made into creative products that have features both in terms of function and aesthetics.. Furniture produced includes chairs, tables, and decorative products. The application of rattan in making table lamps is found on the hood which uses rattan material with a four-axis rattan weaving technique. This technique Arrange the woven material in 4 different directions and will produce a woven pattern with more holes with hexagonal and octagonal pattern shapes. On the lamp body is made of wood glued to the sides of the wood. So the process of making this table lamp is through material observation and experimentation. The purpose of this design is to increase consumer attractiveness to interior needs. The application of rattan material in the design of table lamps produces products that are not only aesthetically pleasing, but also pay attention to aspects of sustainability and functionality needs.

Bio-Pathological Functions of Posttranslational Modifications of Histological Biomarkers in Breast Cancer.

Proteins are the most common types of biomarkers used in breast cancer (BC) theranostics and management. By definition, a biomarker must be a relevant, objective, stable, and quantifiable biomolecule or other parameter, but proteins are known to exhibit the most variate and profound structural and functional variation. Thus, the proteome is highly dynamic and permanently reshaped and readapted, according to changing microenvironments, to maintain the local cell and tissue homeostasis. It is known that protein posttranslational modifications (PTMs) can affect all aspects of protein function. In this review, we focused our analysis on the different types of PTMs of histological biomarkers in BC. Thus, we analyzed the most common PTMs, including phosphorylation, acetylation, methylation, ubiquitination, SUMOylation, neddylation, palmitoylation, myristoylation, and glycosylation/sialylation/fucosylation of transcription factors, proliferation marker Ki-67, plasma membrane proteins, and histone modifications. Most of these PTMs occur in the presence of cellular stress. We emphasized that these PTMs interfere with these biomarkers maintenance, turnover and lifespan, nuclear or subcellular localization, structure and function, stabilization or inactivation, initiation or silencing of genomic and non-genomic pathways, including transcriptional activities or signaling pathways, mitosis, proteostasis, cell-cell and cell-extracellular matrix (ECM) interactions, membrane trafficking, and PPIs. Moreover, PTMs of these biomarkers orchestrate all hallmark pathways that are dysregulated in BC, playing both pro- and/or antitumoral and context-specific roles in DNA damage, repair and genomic stability, inactivation/activation of tumor-suppressor genes and oncogenes, phenotypic plasticity, epigenetic regulation of gene expression and non-mutational reprogramming, proliferative signaling, endocytosis, cell death, dysregulated TME, invasion and metastasis, including epithelial-mesenchymal/mesenchymal-epithelial transition (EMT/MET), and resistance to therapy or reversal of multidrug therapy resistance. PTMs occur in the nucleus but also at the plasma membrane and cytoplasmic level and induce biomarker translocation with opposite effects. Analysis of protein PTMs allows for the discovery and validation of new biomarkers in BC, mainly for early diagnosis, like extracellular vesicle glycosylation, which may be considered as a potential source of circulating cancer biomarkers.

The Free Flap Reconstruction of Facial Defects after Squamous Cell Carcinoma Excision.

Background and Objectives: Cutaneous squamous cell carcinoma is the second most common skin cancer. There are many methods for the reconstruction of facial subunit defects after skin cancer excision. The face is vital to a person's life and should be reconstructed considering functional and aesthetic aspects. Despite a variety of flap types and techniques, it is still challenging to meet the various demands. The aim of this study was to compare free flaps for facial reconstruction after resection of cutaneous squamous cell carcinoma. Materials and Methods: This study included 14 patients from January 2021 to June 2023. Patients who underwent facial SCC resection and subsequent reconstruction using free flaps were analyzed retrospectively. Age, sex, and localization were recorded. Follow-ups ranged from 5 to 21 months, with an average of 13 months. Results: All free flaps survived well except one case of partial flap necrosis. In most patients, good to excellent functional and aesthetic results were obtained. The donor site healed uneventfully in all patients. Conclusions: Free flap reconstruction is an excellent choice in wide skin oncologic defects. In terms of texture, it also could be a good surgical method. The use of a fraxel laser can progressively facilitate improved color matching with the surrounding skin.

Moonlighting Crypto-Enzymes and Domains as Ancient and Versatile Signaling Devices.

Increasing numbers of reports have revealed novel catalytically active cryptic guanylate cyclases (GCs) and adenylate cyclases (ACs) operating within complex proteins in prokaryotes and eukaryotes. Here we review the structural and functional aspects of some of these cyclases and provide examples that illustrate their roles in the regulation of the intramolecular functions of complex proteins, such as the phytosulfokine receptor (PSKR), and reassess their contribution to signal generation and tuning. Another multidomain protein, Arabidopsis thaliana K+ uptake permease (AtKUP5), also harbors multiple catalytically active sites including an N-terminal AC and C-terminal phosphodiesterase (PDE) with an abscisic acid-binding site. We argue that this architecture may enable the fine-tuning and/or sensing of K+ flux and integrate hormone responses to cAMP homeostasis. We also discuss how searches with motifs based on conserved amino acids in catalytic centers led to the discovery of GCs and ACs and propose how this approach can be applied to discover hitherto masked active sites in bacterial, fungal, and animal proteomes. Finally, we show that motif searches are a promising approach to discover ancient biological functions such as hormone or gas binding.

Value modulation of self-defeating impulsivity

BackgroundImpulse control is a critical aspect of cognitive functioning. Intuitively, whether an action is executed prematurely depends on its associated reward, yet the link between value and impulsivity remains poorly understood. Three frameworks for impulsivity offer contrasting views: impulsive behavior may be valuable because it is associated with hidden internal reward (e.g., reduction of mental effort). Alternatively, it can emerge from exploration, which is disadvantageous in the short term, but can yield long-term benefits. Finally, impulsivity may reflect Pavlovian bias, an inherent tendency that occurs even when its outcome is negative. MethodsTo test these hypotheses, we trained seventeen male mice to withhold licking while anticipating variable rewards. We then measured and optogenetically manipulated dopamine release in the ventral striatum. ResultsWe found that higher reward magnitudes correlated with increased impulsivity. This behavior was well explained by a Pavlovian-bias model. Furthermore, we observed negative dopamine signals during premature licking, suggesting that in this task, impulsivity is not merely an unsuccessful attempt at obtaining a reward. Rather, it is a failure to overcome the urge to act prematurely despite knowledge of the negative consequences of such impulsive action. ConclusionOur findings underscore the integral role value plays in regulating impulsivity and suggest that the dopaminergic system influences impulsivity through the mediation of value learning.

What's on the menu? A novel molecular gut content analysis to investigate the feeding behavior of phytophagous insects.

The relationship between phytophagous insects and plants is a central aspect of food webs and ecosystem functioning. The introduction of new species into an environment can have significant impacts on the food web of a native ecosystem. In many cases, there is a lack of knowledge on the biology and feeding behavior of invasive species prior their introduction and in the invaded regions. Gut content analyses of insects have provided valuable information on the host spectrum of insects. However, current approaches are time-consuming and costly. Here, we describe a new molecular gut content analysis (GCA) approach using the Oxford Nanopore (ONT) Flongle sequencing platform to characterize the plant DNA present in the gut of the highly polyphagous insect species Halyomorpha halys. We demonstrate that this technique efficiently amplifies and correctly identifies plant DNA in a mock community. We performed a feeding experiment to determine the sensitivity of this approach and to assess how long the plant DNA can be detected. All plants used in the feeding experiment were correctly identified and detected after 56 days. Surprisingly, we also detected various plant genera that were not included in the feeding experiment and thus were likely ingested months before the experiment. Our study suggests that the GCA using the ONT Flongle sequencing platform represents a rapid and cost-efficient diagnosis of the dietary preferences, host range, and the diversity of consumed plant species of pest insects with high precision.

Downscaling Vapor Pressure Deficit across Complex Forested Terrain

Abstract The forest microclimate shapes many aspects of forest functioning, including plant regeneration and wildfire occurrence. In complex landscapes with varying terrain, the forest microclimate varies at fine spatial scales (10–100 m2). However, accurately mapping this variation remains challenging. Vapor pressure deficit (VPD) is an important microclimatic variable for plant growth and fire activity, yet few studies have specifically focused on downscaling VPD. The aim of this study was to examine the drivers of in-forest VPD in temperate eucalypt forests and develop a model to predict in-forest VPD. We use microclimate data from 37 in-forest weather stations, installed across an aridity gradient in southeastern Australia. We used linear mixed models within an information theoretic approach to develop a predictive model for daily maximum in-forest VPD using open VPD and topographic variables. The highest-ranked model included fundamental topographic drivers of vegetation structure and microclimate in forested landscapes: aspect, elevation, and slope, in addition to open VPD. The model had high accuracy when tested against independent data. We used this model to map fine-scale (30 m2, daily) maximum in-forest VPD across a topographically complex case study landscape. Predicted in-forest VPD showed considerable spatial and temporal variations not captured by coarse-scale open VPD. This represents a significant advancement in our ability to model microclimatic conditions in temperate eucalypt forests and has the potential to advance our understanding of how ecosystem processes vary at fine spatial scales.

Prioritized adjustments in posture stabilization and adaptive reaching during neuromuscular fatigue of lower-limb muscles.

Neuromuscular fatigue (NMF) induces temporary reductions in muscle force production capacity, affecting various aspects of motor function. Although studies have extensively explored NMF's impact on muscle activation patterns and postural stability, its influence on motor adaptation processes remains less understood. This article investigates the effects of localized NMF on motor adaptation during upright stance, focusing on reaching tasks. Using a force-field perturbation paradigm, participants performed reaching movements while standing upright before and after inducing NMF in the ankle dorsiflexor muscles. Results revealed that despite maintained postural stability, participants in the NMF group exhibited larger movement errors during reaching tasks, suggesting impaired motor adaptation. This was evident in both initial and terminal phases of adaptation, indicating a disruption in learning processes rather than a decreased adaptation rate. Analysis of electromyography activation patterns highlighted distinct strategies between groups, with the NMF group showing altered activation of both fatigued and nonfatigued muscles. In addition, differences in coactivation patterns suggested compensatory mechanisms to prioritize postural stability despite NMF-induced disruptions. These findings underscore the complex interplay between NMF, motor adaptation, and postural control, suggesting a potential role for central nervous system mechanisms in mediating adaptation processes. Understanding these mechanisms has implications for sports performance, rehabilitation, and motor skill acquisition, where NMF may impact the learning and retention of motor tasks. Further research is warranted to elucidate the transient or long-term effects of NMF on motor adaptation and its implications for motor rehabilitation interventions.NEW & NOTEWORTHY We assessed motor adaptation during force-field reaching following exercise-induced neuromuscular fatigue (NMF) on postural muscles. NMF impaired adaptation in performance. Similarly, diverging activation strategies were observed in the muscles. No effects were seen on measures of postural control. These results suggest the remodulation of motor commands to the muscles in the presence of NMF, which may be relevant in settings where participants could be exposed to NMF while learning, such as sports and rehabilitation.

Amelioration of dextran sodium sulphate-induced colitis in mice by treatment with Lactobacillus rhamnosus and Lactobacillus reuteri: intraspecific and interspecific patterns

Lactobacillus rhamnosus (Rh) and Lactobacillus reuteri (Re) are well-known probiotic species in inflammatory bowel disease (IBD) research. The variations between these species’ efficacy against colitis, and their model of action in this regard, are intriguing and enable treatment to be individually tailored to patients. In this study, four strains each of Rh and Re were isolated from fecal samples and their draft genomes were sequenced. The anti-colitis activities of both strains involved various aspects of intestinal immune, physical, chemical, and biological barrier function. Strikingly, the tested strains exhibited considerable interspecies and intraspecies specificity in colitis amelioration. Rh strains significantly outperformed Re strains in terms of short-chain fatty acid synthesis. Nevertheless, Re strains were more effective than Rh strains in inhibiting production of inflammatory factors; promoting production of intestinal mucus, antimicrobial peptides, and tight junction proteins; and supporting the stem cell compartment. This accounts for the anti-colitis outcomes of Re strains being superior to those of Rh strains. In addition, the effective Rh and Re strains were found to express high concentrations of specific carbohydrate metabolism- and prophage-related genes, respectively. Taken together, the results of this study could assist researchers in developing effective therapies for IBD.