Abstract: This study investigates the impact of Artificial Intelligence (AI) CHATBOTS on users' empathic abilities and emotional intelligence (EI). The study includes a comparative analysis of people who use and don't use AI CHATBOTS for emotional support. The objectives included assessing how the effect of AI CHATBOTS on empathy affects users' empathic responses, investigating changes in emotional intelligence associated with the use of AI CHATBOTS, and identifying factors contributing to the effectiveness of AI CHATBOTS in improving empathy ability. The method involved a comparative study of 140 participants between the ages of 18 and 25, divided into users and non-users of an AI CHATBOT aimed at providing emotional support. Data collection included self-report surveys measuring CHATBOT usage, Interpersonal Reactivity index (IRI), and emotional intelligence (EI). Statistical analysis reveals significant differences in scores for perspective taking, fantasy, and personal distress between users and non-users of AI CHATBOTS, suggesting that technology-mediated empathy may be associated with specific aspects of empathic responses. Qualitative analysis of participants' responses reveals different experiences and perceptions related to the use of AI CHATBOTS, such as emotional and academic support and platform choice (e.g., CHATGPT, SNAPCHAT). The findings provide valuable insight into the complex relationship between technology-mediated empathy and human emotional responses. In conclusion, this study highlights the importance of considering the role of technology in shaping human interactions and emotional experiences. This result suggests that exposure to AI CHATBOTS may influence empathic responses and aspects of emotional intelligence. However, further research is recommended to explore the underlying mechanisms and ethical considerations related to AI-mediated empathy.