Asian Otters Research Articles

NO PROGRESSION OF UROLITHS IN ASIAN SMALL-CLAWED OTTERS (AONYX CINEREUS) FED A NATURALISTIC CRUSTACEAN-BASED DIET FOR 2 YEARS.

Asian small-clawed otters (ASCO; Aonyx cinereus) are a popular species of otter housed in zoological institutions. A common health challenge in this species is the development of uroliths, which may have a dietary origin. Feeding recommendations for ASCO are largely based on the nutrient requirements of domestic carnivore models. Using otter-specific feeding ecology may allow for further refinement of these nutrient recommendations. This study aimed to assess if a naturalistic diet of crustaceans, mollusks, and fish could control the development of uroliths in ASCO. Baseline data were collected on 10 ASCO (five males and five females) of different ages and repeated 2 years after the treatment diet was introduced. Blood and urine parameters, as well as the size of nephroliths based on radiographic images, were recorded. The treatment diet was higher in protein and lower in calcium than the nontreatment diet and did not contain any kibble. During the 2-year treatment trial, blood globulin, glucose, and sodium increased and albumin decreased. Glucosuria and leukocyturia significantly decreased. There were no significant changes in urolith size over the 2 years, and animals without nephroliths at the beginning of the study remained urolith free. The development of nephroliths was significantly reduced during the treatment compared with the year prior. Although interpretation is limited by the methods used, a naturalist diet may have a beneficial impact in the development of uroliths in ASCO.

Captive Asian short-clawed otters (Aonyx cinereus) learn to exploit unfamiliar natural prey.

Foraging plays a vital role in animal life histories, and learning whether unfamiliar food items are palatable is a key part of this process. Animals that engage in extractive foraging must also learn how to overcome the protective measures of their prey. While otters (subfamily Lutrinae) are a taxon known for their extractive foraging behaviour, how they learn about prey palatability and acquire extractive foraging techniques remains poorly understood. Here we investigated (i) how captive Asian short-clawed otters (Aonyx cinereus) learned to interact with, and extract meat from, unfamiliar natural prey and (ii) how their exploitation of such prey compared to their ability to overcome artificial foraging tasks containing familiar food rewards. Network-based diffusion analysis showed that otters learned to interact with unfamiliar natural prey by observing their group mates. However, once interacting with the prey, they learned to extract the meat mainly asocially. In addition, otters took longer to overcome the protective measures of unfamiliar natural prey than those of extractive food puzzles. Asian short-clawed otter populations are declining in the wild. Increasing our understanding of how they learn to overcome novel foraging challenges could help develop pre-release training procedures as part of reintroduction programmes for otter conservation.

Dirofilaria immitis in an Asian small-clawed otter (Aonyx cinereus) from southeastern Louisiana, United States.

A single, male Dirofilaria immitis was found in the right ventricle of a captive, 10-month-old female Asian small-clawed otter (Aonyx cinereus) from East Feliciana parish in Louisiana, USA. Molecular analysis was performed for unequivocal species level identification using the cytochrome c oxidase subunit 1 (cox1) region of the mitochondrial DNA and comparing to known D. immitis cox1 sequences available on GenBank to which the specimen had a 99.54-100% pair wise identity. As there is no safe adulticidal treatment for D. immitis in mustelids, chemoprophylaxis using macrocyclic lactones is recommended to prevent infection. Further studies are needed to better characterize D. immitis infection and its propensity to cause disease in the Asian small-clawed otter.

Toxoplasmosis in Zoo Animals: A Retrospective Pathology Review of 126 Cases.

Simple SummaryToxoplasma gondii is widespread amongst domestic animals and can affect humans. Whilst the disease is of economic importance and well-studied in ruminants, this is the first large-scale study evaluating toxoplasmosis in zoo animals. The aim of this study was to identify species that are particularly susceptible to disease, review clinical symptoms, organ distribution and key target tissues, highlight unusual outbreaks/presentations, assess predilections, evaluate toxoplasmosis as the cause of death and consider seasonality. A total of 31 species were represented, with ring-tailed lemurs (RTLs), meerkats, Pallas’ cats and squirrel monkeys most affected. An unusual outbreak occurred in Asian short-clawed otters. Non-specific, neurological, gastrointestinal, respiratory signs and sudden death dominated clinically, mainly in animals over 12 months of age that presented with systemic disease. Pallas’ cats showed encephalitis (inflammation of the brain) and lymphoid tissue was frequently involved in RTLs. Lesions were primarily found in heart, liver, lungs, brain, spleen and lymph nodes. Cases occurred year-round, with species-specific peaks and increases between August and November. This study demonstrates that toxoplasmosis is a significant cause of disease in zoo animals. Knowledge of species susceptibility will aid the treatment of affected animals by zoo veterinarians. Due to the lifecycle of the parasite, feral cat/rodent control is crucial to reduce infection pressure and prevent disease.Toxoplasma gondii is an extremely successful zoonotic protozoan parasite that has been demonstrated in a wide range of endo- and poikilothermic species. Although infection is widespread amongst domestic animals, overt disease other than abortion in small ruminants is sporadic. This survey evaluates toxoplasmosis in zoo animals based on a systematic review of pathology archive material (n = 33,506 submissions) over a 16-year study period. A total of 126 submissions, deriving from 32 zoos, two educational facilities and two private owners, were included in the study, based on gross lesions, cytological, histological and immunohistological diagnosis of toxoplasmosis. Clinical history, signalment, annual distribution and post-mortem findings were evaluated. A total of 31 species (mammalian 97%/avian 3%) were represented in the study material. Ring-tailed lemurs, slender tailed meerkats, Pallas’ cats, and squirrel monkeys were most affected. An unusual outbreak occurred in Asian small-clawed otters, in which toxoplasmosis has not been reported to date. Clinically, animals over 12 months of age presented with non-specific symptoms (anorexia, weight loss, lethargy, debilitation), neurological, gastrointestinal or respiratory signs and sudden death. Systemic disease predominated, with a propensity for encephalitis in meerkats and Pallas’ cats and systemic disease involving lymphoid tissues in ring-tailed lemurs. Cases in the UK occurred year-round, with species-specific peaks and increases between August and November. This study reinforces the importance of toxoplasmosis as a significant cause of sporadic and epizootic mortalities in a wide range of zoo animals. Feral cat control is crucial to reduce infection pressure.

The existence of Asian small-clawed otter (Aonyx cinereus Illiger, 1815) in the UCPS Hydropower, Cianjur, West Java, Indonesia

Abstract. Dirgantara AP, Megantara EN, Husodo T, Febrianto P, Wulandari I, Shanida SS. 2021. The existence of Asian small-clawed otter (Aonyx cinereus Illiger, 1815) in the UCPS Hydropower, Cianjur, West Java. Biodiversitas 22: 4391-4401. Little information about the existence of small-clawed otters (Aonyx cinereus) in Indonesia is available. Small-clawed otter is the top predator and biological indicator of wetland health. However, the small-clawed otter population in the world has decreased due to anthropogenic factors. This study aims to reveal 1) estimate the population size of small-clawed otters and 2) the small-clawed otter signs at the UCPS Hydropower construction site, Cianjur, West Java. This study used a qualitative approach in February-April 2017. Sign surveys (feces, footprints, leftovers, and dens) and camera trapping were applied in this study. There were 32 signs with details of 21 feces, eight footprints, and three dens. The estimation of small-clawed otter population size at UCPS Hydropower ranges from 5-14 individuals in total scattered in the Citali-Cisokan, Cilengkong, and Cirumamis rivers. In addition, the discovery of infant and bedding repair activities shows that the habitat in the UCPS Hydropower area supports the daily needs of small-clawed otters, especially in terms of breeding and shelter. Further studies are needed regarding the impact of the UCPS Hydropower construction on the existence of small-clawed otters, especially in the overlapping between small-clawed otter habitat and the UCPS Hydropower construction sites, by monitoring and making wildlife management policies.

Global change on the roof of the world: Vulnerability of Himalayan otter species to land use and climate alterations

AbstractAimClimate Change Vulnerability Assessment (CCVA) prescribes the quantification of species vulnerability based on three components: sensitivity, adaptive capacity and exposure. Such assessments should be performed through combined approaches that integrate trait‐based elements (e.g., measures of species sensitivity such as niche width) with correlative tools quantifying exposure (magnitude of changes in climate within species habitat). Furthermore, as land use alterations may increase climate impacts on biodiversity, CCVAs should focus on both climate and land use change effects. Unfortunately, most of such assessments have so far focused exclusively on exposure to climate change.LocationHimalaya.MethodsWe evaluated the vulnerability of three otter species occurring in the Himalayan region, that is, Aonyx cinereus, Lutra lutra and Lutrogale perspicillata, to 2050 climate and land use change through the recently proposed Climate Niche Factor Analysis (CNFA) framework combined with Species Distribution Models.ResultsFuture climate and land use change will reduce (6%–15%) and shift (10%–18%) the geographical range of the three species in the Himalaya, with land use alterations exerting far more severe effects than climate change. Among vulnerability components, sensitivity played a greater role than exposure in determining the vulnerability of the otters. Specifically, the most specialist species, L. perspicillata, showed the highest vulnerability in comparison with the most generalist, L. lutra.Main conclusionsOur results underline how coupling climate and land use change components in CCVAs can generate diverging predictions of species vulnerability compared to approaches relying on climate change only. Moreover, intrinsic components, such as species sensitivity, proved significantly more important in determining vulnerability than extrinsic metrics such as habitat exposure.

New canine parvovirus 2a infection in an imported Asian small-clawed otter (Aonyx cinereus) in Japan.

Post-import from the Republic of Indonesia to Japan in 2017, two juvenile, captive bred Asian small-clawed otters (Aonyx cinereus) exhibited gastrointestinal symptoms, including vomiting, diarrhea, and hematemesis, and died. One of them was examined postmortem. Microscopically, the small intestinal mucosa was necrotic with crypts lined by regenerating large epithelial cells. A gastric cardiac mucosal ulcerative lesion containing fungal yeasts and pseudohyphae morphologically indicated Candida spp. The lymph nodes exhibited marked lymphoid depletion. Canine parvovirus 2 (CPV-2) was isolated from an oral swab, and virus protein 2 (VP2) gene sequencing revealed new CPV-2a. To our knowledge, this is the first new CPV-2a infection report in Asian small-clawed otters. This infection should be considered in gastrointestinal symptom-related cases in this species.

Effects of food type and abundance on begging and sharing in Asian small-clawed otters (Aonyx cinereus).

Begging for food, a conspicuous solicitation display, is common in a variety of taxa, and it has received extensive research attention in a parent-offspring context. Both theoretical models and empirical evidence suggest that offspring begging can be an honest signal of hunger or a mediator of competition between siblings. At a behavioural mechanistic level, begging for food can be a form of harassment aimed at persuading those in possession of food to share. Food sharing, defined as the transfer of a defendable food item from one individual to another, can vary considerably between species, age-classes and food type and abundance. We investigated the determinants of begging and food-sharing behaviours in Asian small-clawed otters (Aonyx cinereus), a group-living species that commonly exhibits begging in captivity. We presented two captive otter populations with three food types that varied in exploitation complexity, in three different abundances. We predicted that begging rates would be highest when food was in lowest abundance and hardest to exploit, and that increased begging would lead to increased food sharing. We found that, over time, increased begging rates were indeed correlated with increased food transfers, but neither food type complexity nor abundance affected begging or sharing rates. However, age category was significantly associated with begging and food sharing rates: juvenile otters begged more and shared less than adult otters. The results from this first experimental study on begging and food sharing within the Mustelid family begin to reveal some of the drivers of these behaviours.

Learning strategies and long-term memory in Asian short-clawed otters (Aonyx cinereus).

Social learning, where information is acquired from others, is taxonomically widespread. There is growing evidence that animals selectively employ ‘social learning strategies', which determine e.g. when to copy others instead of learning asocially and whom to copy. Furthermore, once animals have acquired new information, e.g. regarding profitable resources, it is beneficial for them to commit it to long-term memory (LTM), especially if it allows access to profitable resources in the future. Research into social learning strategies and LTM has covered a wide range of taxa. However, otters (subfamily Lutrinae), popular in zoos due to their social nature and playfulness, remained neglected until a recent study provided evidence of social learning in captive smooth-coated otters (Lutrogale perspicillata), but not in Asian short-clawed otters (Aonyx cinereus). We investigated Asian short-clawed otters' learning strategies and LTM performance in a foraging context. We presented novel extractive foraging tasks twice to captive family groups and used network-based diffusion analysis to provide evidence of a capacity for social learning and LTM in this species. A major cause of wild Asian short-clawed otter declines is prey scarcity. Furthering our understanding of how they learn about and remember novel food sources could inform key conservation strategies.

Conservation status, ex situ priorities and emerging threats to small carnivores

Madagascar contains the highest concentration of threatened carnivore species anywhere in the world. The country's deteriorating protected‐area network makes it a priority for small carnivore conservation, and several species may need some form of ex situ action. Small carnivores in South East Asia are under significant pressure from hunting to supply the illegal wildlife trade, as well as habitat loss, and the interactions between these two factors. Owston's civet Chrotogale owstoni is the conservation priority from this region. The rapidly declining status of Large‐spotted civet Viverra megaspila needs careful monitoring, as it may warrant intervention soon. Otters (Lutrinae) in Asia are in decline and there may exist as yet undescribed conservation units that are extremely threatened. Hairy‐nosed otter Lutra sumatrana is not yet a priority for ex situ conservation because of low feasibility; efforts should focus on managing the populations of Smooth‐coated otter Lutrogale perspicillata and Asian small‐clawed otter Aonyx cinereus already in human care. The future of European mink Mustela lutreola and Black‐footed ferret Mustela nigripes will depend on ongoing support to conservation‐breeding efforts, as well as threat reduction. To a lesser extent, Red panda Ailurus fulgens conservation is also dependent on continued support to its integrated conservation plan. There are no obvious candidate species for ex situ conservation of small carnivores from Africa, though the bushmeat trade, planned large‐scale infrastructure projects and a growing transnational wildlife trade link to Asia may change this assessment in the near future. It is unclear what role ex situ conservation would have for Pygmy raccoon Procyon pygmaeus: the main threat to this species is hurricanes. A speculative assessment of emerging threats to small carnivores includes: infrastructural development in the tropics, illegal wildlife trade networks that include Latin American, African and Asian markets, and the growing Vietnamese and Chinese diasporas that operate in other Asian countries, Africa and Latin America, and the interactions between all of these factors.

Volunteering for otter conservation in Nepal: communicating with local communities

Amongst three species of otters, the Asian Small-clawed Otter has not been recorded since Hodgson (1839). Since 1991, the Eurasian Otter has neither been photographed nor sighted in the country. Recently, the Smooth-coated Otter has been reported only from Bardia National Park and its west. In this chaotic situation, a volunteer project was initiated for otter conservation in Nepal three years ago. 1000 copies of an otter conservation awareness poster were printed in 2017 and a second set of posters in 2019. Posters were distributed to local communities while communicating with the local people along the way and during stays. Thus far, we have conducted awareness activities at selected sites in nine districts and four protected areas. Over three years, we have talked with more than 2000 local people about otters and disseminated more than 1300 posters. At all sites, local communities responded that they had not seen otters for 20 to 30 years or more and for the last 10 years, even in protected areas. However, there are hopes that the last scarce individuals or populations of otters remain at very few sites. Overfishing, river poisoning and pollution, hydropower dams and station construction, and sand and pebble extraction from rivers, are the major causes for depletion of otters in these areas. In the past, trapping by Tibetans for otter pelts and by nomads from the plains of Nepal and India were major causes for population declines. In conclusion, we convey a conservation message that humans must learn to co-exist with otters if the species are to survive.

Molecular DNA-based spatial mapping technique predicting diversity and distribution of otters (Lutrinae) in Peninsular Malaysia using non-invasive fecal samples

This study investigated the current distribution of otters (Lutrinae) in all states of Peninsular Malaysia. Four species of otters have been recorded in Peninsular Malaysia in the past based on field survey methodology, namely the Asian small-clawed otter (Aonyx cinereus), smooth-coated otter (Lutrogale perspicillata), hairy-nosed otter (Lutra sumatrana), and Eurasian otter (Lutra lutra). This study aims to determine the current number of otter species and their distribution in Peninsular Malaysia through molecular approaches using D-loop and Cyt b loci of mitochondrial DNA sequences. Our findings indicate that 337 samples were identified as three species of otter that are currently distributed in Peninsular Malaysia: A. cinereus (112 samples), L. perspicillata (218 samples), and L. sumatrana (7 samples). All three species can be found in Perak, Kelantan, Pahang, Selangor, and Johor while other states only contained two species (A. cinereus and L. perspicillata). Seven sympatric locations were recorded between two or three species: Tumpat and Kg. Padang Salim (Kelantan), Penarik and Sg. Kemaman (Terengganu), Taman Negara (Pahang), Tg. Piai (Johor), and Kuala Gula (Perak). The discovery of L. sumatrana in this study has confirmed that this species is still present in Peninsular Malaysia. However, L. lutra was not observed in this study. Our findings successfully highlight the diversity and distribution of Peninsular Malaysian otters that suggest the importance of continuous assessment and population status of otters to ensure their ongoing survival in Peninsular Malaysia.

Effect of visitors on the behaviour of three Asian small‐clawed ottersAonyx cinereusat Cattolica Aquarium

The Asian small‐clawed otterAonyx cinereusis classified as Vulnerable by the International Union for Conservation of Nature Red List. The aim of this study was to evaluate the effects of the presence of visitors on the behavioural repertoire of three Asian small‐clawed otters at Cattolica Aquarium, Italy. Observational data sampling took place over two periods of 11 days each. The first period was in August when the Aquarium was open and there were visitors present (open period = OP). The second period was between September and October, on days when the Aquarium was closed to visitors (closed period = CP). Focal sampling was integrated with behaviour sampling during data collection. To evaluate the significant differences in frequency and duration of behaviours during the OP and CP periods, a Wilcoxon Signed Rank test was performed. The results of this study showed that the absence of visitors stimulated significant differences in frequency and duration of activity (such as ‘locomotion’ and ‘waiting’) and social behaviours (such as ‘play’). The otters also spent significantly more time visible than ‘out of sight’ when there were no visitors present. This preliminary research provides information that may influence the way zoos and aquatic parks manage their otters, and contributes to the improvement of the welfare of animals in human care.

The drivers and functions of rock juggling in otters.

Object play refers to the seemingly non-functional manipulation of inanimate items when in a relaxed state. In juveniles, object play may help develop skills to aid survival. However, why adults show object play remains poorly understood. We studied potential drivers and functions of the well-known object play behaviour of rock juggling in Asian small-clawed (Aonyx cinereus) and smooth-coated (Lutrogale perspicillata) otters. These are closely related species, but Asian small-clawed otters perform extractive foraging movements to exploit crabs and shellfish while smooth-coated otters forage on fish. We thus predicted that frequent rock jugglers might be better at solving extractive foraging puzzles in the first species, but not the latter. We also assessed whether species, age, sex and hunger correlated with rock juggling frequency. We found that juvenile and senior otters juggled more than adults. However, rock juggling frequency did not differ between species or sexes. Otters juggled more when ‘hungry’, but frequent jugglers did not solve food puzzles faster. Our results suggest that rock juggling may be a misdirected behaviour when hungry and may facilitate juveniles' motor development, but it appears unrelated to foraging skills. We suggest future studies to reveal the ontogeny, evolution and welfare implications of this object play behaviour.

Spatiotemporal Patterns of Latrine-Site Use by Small-Clawed Otters in a Heterogeneous Rice Field Landscape

Tropical rice fields with asynchronous growth stages provide a good model to investigate the effects of landscape heterogeneity on the use of agricultural landscapes by wildlife. The small-clawed otter (Aonyx cinereus) uses rice fields as latrine sites in Southeast Asia. Through a 53-week survey of latrine sites, we analyzed the spatiotemporal patterns of latrine-site use by small-clawed otters in an asynchronous rice field in West Sumatra, Indonesia. Based on a generalized linear mixed model incorporating local environmental factors, otter latrine-site visitation was positively associated with the vegetative stage of rice cultivation and the biomass of a major prey item, the golden apple snail (Pomacea canaliculata). The vegetative stage of rice cultivation and golden apple snail biomass were closely associated with deep water in rice fields; therefore, water availability in rice fields is likely important in determining the spatiotemporal patterns of small-clawed otter latrine-site use in the asynchronous rice field landscape. To consistently provide latrine sites for small-clawed otters, we recommend that asynchronous rice cultivation be maintained, or zoning of synchronous and asynchronous rice cultivation areas be planned in the region.

The first record of a wild hypopigmented oriental small-clawed otter (Aonyx cinereus)

Hypopigmentation, whose most common forms include albinism and leucism, results in atypical coloration and occurs throughout the animal kingdom. During camera trapping in Bukit Barisan Selatan National Park, Sumatra, we documented the first record of hypopigmentation in oriental small-clawed otters (Aonyx cinereus). Hypopigmentation is rarely documented in the literature, and this appears to be the second record for any otter species.

Assessing the distribution pattern of otters in four rivers of the Indian Himalayan biodiversity hotspot

Abstract The Eurasian otter (Lutra lutra), smooth‐coated otter (Lutrogale perspicillata), and Asian small‐clawed otter (Aonyx cinereus) have all been reported previously from the Indian state of Uttarakhand. However, little information is available about their current distribution in a mountainous region that is subject to increasing human‐induced stressors (such as hydropower plants, pollution, sand and boulder mining, destructive fishing techniques, poaching). Owing to important roles played by these otters in structuring riverine food webs (particularly taking account of their roles as top carnivores), it is critical that they receive suitable protection in the face of projected temperature rise, change in precipitation patterns, and associated river flows in this Himalayan biodiversity hotspot. This study assesses otter distribution in four rivers of Uttarakhand as a basis for informing future conservation actions. Field surveys were conducted (October 2018–January 2019) in reaches of the Kosi, Ramganga, Khoh, and Song rivers, supported by semi‐structured interviews (N = 379) conducted with members of local communities to collect qualitative data on views and perceptions of otter species. In addition, community‐based otter awareness camps were organized for local youths (N = 105), adults (N = 115), and schoolchildren (N = 256 covering 10 schools). Habitat suitability maps were created using remote‐sensing data, survey findings, and a geographic information system to provide information about priority reaches of river to be targeted for future conservation efforts. This study provides critical interdisciplinary baseline information to guide decision‐makers towards developing a targeted, otter‐specific conservation programme for this important Himalayan biodiversity hotspot. The otter conservation education programmes conducted during this study resulted in a proposal to set up a community‐based conservation initiative to monitor and report otter sightings from the area, potentially representing a way forward for achieving simultaneous otter conservation and associated ecosystem benefits for local communities.

Investigation of ancient DNA to enhance natural history museum collections: misidentification of smooth-coated otter (Lutrogale perspicillata) specimens across multiple museums

Historical and modern natural museum collections are storehouses of extraordinary value for scientific research in a wide range of fields. Recent advances in molecular biotechnology (e.g., next generation genomics) have increased the range of collection material employable for DNA-based analyses to unprecedented levels. Nevertheless, the value of museum specimens strictly depends on reliability of data associated with them. We report on investigations of ancient DNA from specimens of smooth-coated otter (Lutrogale perspicillata, Mustelidae), the largest otter species living in Asia, in US and European mammal collections. Mitochondrial DNA Cytochrome-b gene sequencing proved that the studied specimens were not the expected taxon. Indeed, they actually belonged to three different species, namely the Asian small-clawed (Aonyx cinereus), Eurasian (Lutra lutra) and African clawless (Aonyx capensis) otters. This represents the first record of mustelid misidentification from museum collections. Detection of errors can be extremely difficult when based only on collectors’ notes and data. Hence, we warn scientists involved in otter research about potential challenges when dealing with museum specimens. We recommend curators pursue a multidisciplinary approach, including DNA analyses, to accurately catalogue the resources under their management and uphold the value of biodiversity information.

Mammals diversity of mount sawal wildlife reserve, Ciamis, West Jawa

We conducted research on mammals diversity in the Mount Sawal Wildlife Reserve on March 2012. Data collection was obtained by direct observation and interviews. They are includes direct encounters, traces/signs of the mammals presence (ex: faeces, traces/footprints, odors, scratch marks, food scraps, etc.) as well as information from the community around the forest. Observations were carried out in seven observation path/forest block in Mount Sawal namely Belandongan, Cibaruyan Jero, Gintung, Gunung Luhur, Kigugula, Jamikuwu, and Sawah Gunung. We documented at least 10 species of mammals have been recorded, including Panthera pardus melas, Presbytis comata, Trachypithecus auratus, Macaca fascicularis, Muntiacus muntjak, Hystrix javanica, Aonyx cinereus, Prionailurus bengalensis, Mydaus javanensis, and Sus Scrofa. The existence of mammals is relatively evenly distributed in all observation path/forest block in The Sawal Mountain. Six species are protected by Indonesian Law, including Panthera pardus melas, Presbytis comata, Trachypithecus auratus, Muntiacus muntjak, Hystrix javanica, and Prionailurus bengalensis. Information on the diversity of mammals is expected to be a scientific consideration in the management area of the Mount Sawal Wildlife Reserve.

Management of nephrolithiasis by pyelotomy and pyeloscopy in an Asian small-clawed otter (Aonyx cinereus).

A captive adult sexually intact female Asian small-clawed otter (Aonyx cinereus) was evaluated because of diarrhea and signs of abdominal discomfort on arrival at the quarantine area of a zoological park. Radiographic and ultrasonographic examinations revealed the presence of a large nephrolith in the left kidney. Mild nephrolithiasis was detected in the right kidney. Results of hematologic and serum biochemical analyses were within references intervals. Intravenous pyelography was used for functional assessment of the urinary system and revealed normal renal excretory function bilaterally. Unilateral (left-sided) pyelotomy was selected as a treatment option to preserve maximal functional renal tissue. Pyeloscopy was successfully used for detection of some small nephroliths that were not removed by repeated flushing of the renal pelvis. Surgical treatment of the right kidney was not considered necessary. The otter recovered fully from anesthesia and surgery. Renal function was considered normal when assessed at 3 and 7 days and at 1 and 12 months after surgery. To the authors' knowledge, pyelonephrotomy in otters has not been reported and may be considered as a potential surgical solution for nephrolithiasis. Pyeloscopy allowed the identification of small uroliths that were not removed by repeated flushing of the left renal pelvis following removal of the large nephroliths.