Paradoxurus Hermaphroditus Research Articles

A Study on the Potential of Mammalian Feces as Seed Dispersal Agent in a Disturbed Forest Landscape

Mammalian feces are digestive residuals that come out through the anal in the form of materials that is partially or completely digested. Mammals have the potential to disperse seeds and regenerate forests through feces. However, quantitative studies on the potential of various species of mammals need to be done to clarify such a role. We collected mammalian feces through field surveys in forest habitat, forest edges and along the riverbank at Solok Selatan, West Sumatra. Seed viability test was carried out at the Laboratory of Plant Physiology and Greenhouse of the Department of Biology, Andalas University. We successfully collected 31 piles from eight species of mammals belonging to three orders and six families. Seeds of four plant species were found in 63% of mammalian feces, all of which are from the Viverridae family (Paradoxurus hermaphroditus, Arctogalidia trivirgata, Arctictis binturong). The piles were more often (80.6%) found at the forest edge than at riverside and forests. Seed viability tests showed that all seeds failed to germinate. The results of this study indicate that Viverridae has the potential for seed dispersal but viability test does not support the role of mammals for forest regeneration through germination.

Diversity of Urban Mammals in Bharatpur Metropolitan City, Chitwan, Nepal

The study of diversity of mammals was accomplished in the urban areas of Bharatpur Metropolitan City, a prime location of Nepal and confined in 433 km2; consists tropical climate and harbor variety of wildlife since the location is connected with Narayani River, community forests and Chitwan National Park. During data collection by direct observation, hearing sound calls and land marks left, the study was conducted from January to July 2016 for six months. Altogether, 12 families with 28 species of mammals were recorded in which 53.75% (15/28) were Temporary Living (TL) and 46.42% (13/28) were Permanent Living (PL). Out of 12 families; Soricidae had the highest, 21.43% (6/28) of species; followed by Cercopithecidae, Cervidae and Vespertilionidae, 10.71% (3/28); Canidae, Felidae, Herpestidae, Muridae, and Viverridae, 7.14% (2/28); and Mustelidae, Rhinocerotidae, and Suidae with 3.57% (1/28) each. Total Five different IUCN categories; Endangered (EN), Vulnerable (VU), Near Threatened (NT), Data Deficient (DD) and Least Concerned (LC) were closely observed. Among them, LC had the highest, 64.29% (18/28) of species followed by VU, 21.43% (6/28); EN, 7.14% (2/28); and CR, DD equally with 3.57% (1/28). Paradoxurus hermaphroditus pallasi, is an Asian Palm Civet, which is reported for the first time from Bharatpur, Chitwan, Nepal. The possible factors responsible to attract wildlife in urban areas were availability of seasonal cultivated crops, fruiting and flowering plants; and household organic wastes. But; the increasing traffics stray dogs, and lack of hidings led to threat to wild mammals. The cutting down of trees in the private areas and frequent road killings also amplify risk of small wild mammals.

A NON-INVASIVE TECHNIQUE USING TO MONITOR REPRODUCTIVE HORMONES IN COMMON PALM CIVETS

Fecal steroid assays have been used to study and provide information on the estrous cycle, pregnancy, re-estrus, reproductive season and therapeutic treatments in an expanded list of species. The purpose of the present study was to monitor the reproductive status of the Commom Palm Civets (Paradoxurus hermaphroditus) by fecal steroid assays. The study was conducted to collect 2635 fecal samples from 12 adult female civets in Dong Nai Biotechnology Center. The fecal contents of Progesterone (P4) and Estradiol (E2) were determinned by using fully automatic ELISA Dynex DS2 (Dynex, USA), Progesterone and Estradiol ELISA Kit (DRG International, Inc., Germany). In non-pregnancy civets, the concentrations of fecal E2 ranged from 0.05 to 7.01 μg/g df, with an average of 1.07 ± 0.84 μg/g and a peak of 3.22 ± 0.64 μg/g. Fecal progesterone metabolites were from 0.15 to 12.32 μg/g, the overall mean of the samples was 1.72 ± 2.16 μg/g. The period of change in E2 content averaging was 28.6 ± 2.29 days. During pregnancy, the P4 content in the stool ranged from 6.21-23.12 μg/g, an average of 15.17 ± 5.22 μg/g and approximately 5 to 7 fold higher than non-pregnant (P

Periodic resource scarcity and potential for interspecific competition influences distribution of small carnivores in a seasonally dry tropical forest fragment

Small, isolated, and disturbed forest fragments potentially offer valuable habitats for small carnivore conservation. Yet the influence of resource availability—critical for survival and reproduction—on small carnivore habitat use within these modified environments is poorly understood. We conducted camera-trap surveys within a seasonally dry tropical forest fragment (148 km2) in northeastern Thailand to better understand the influence resource availability has on small carnivore habitat use within fragmented areas. Species occupancy models including both habitat and resource variables were assessed across periods of high and low resource availability (fruit abundance and rodent biomass). Species with similar resource or habitat preferences may also compete when resources are low. Therefore, we tested species with shared preferences for patterns of spatial co-occurrence and temporal overlap. Fruit availability influenced the distribution of common palm civet (Paradoxurus hermaphroditus), while rodent biomass influenced Asiatic jackal (Canis aureus), leopard cat (Prionailurus bengalensis), and small Indian civet (Viverricula indica), but only during annual periods of scarcity. In contrast, small Asian mongoose (Herpestes javanicus) was highly selective for a specific habitat (dry dipterocarp forest) regardless of seasonal fluctuations in food availability. Occupancy increased for all species during periods of resource scarcity, with leopard cat and Asiatic jackal experiencing the greatest increases (139% and 58%, respectively). Species with shared resource and habitat preferences appeared to avoid each other, either spatially or temporally. For example, leopard cat and Asiatic jackal co-occurred spatially less than would be expected, though only when rodents were scarce. Similarly, small Indian civet and small Asian mongoose, which used the same habitat and co-occurred spatially at a rate greater than expected, were active at different times. Our results indicate that seasonal resource fluctuations and interspecific interactions strongly influence the distribution of sympatric small carnivores in a fragmented forest.

Glucose levels and Stress Index of Common Palm Civet (Paradoxurus hermaphroditus) Coffee Eaters and not Coffee Eaters

Common palm civet is a mamalia which often used to produce luwak coffee. Therefore health status of common palm civet is very important to be considered because force feeding of coffee can turn to stress condition. Glucose level, leukocyte profile and ratio N/L were often used as stress indicator. This study was aimed to determine glucose levels from blood of common palm civet, determine leukocyte profile and its deffirentiation also to calculate neutrophyl/limpocyte ratio from six common palm civet there were three group of observation, there were common palm civet coffe eaters and not coffe eaters. Blood samples were taken intracardially for further analyzed using manual blood method. Glucose levels of coffee eaters common palm civet was higher than common palm civet that was not coffee eaters. Stress index of coffee eaters common palm civet (0,516) was higher than not coffee eaters (0,463), but it was still in the normal range. Common palm civet that eat coffee continuosly can lead to oxidative stress condition.

MORFOLOGI KELENJAR ANAL MUSANG LUAK BETINA (PARADOXURUS HERMAPHRODITUS)

The common palm civet (Paradoxurus hermaphroditus) is one of the mammalian in Indonesia which have anal gland in female and fefemale that located in the lower tail. This research was aimed to describe the morphology of anal gland of common palm civet female. One female of common palm civet was used in this research which observed macroscopic and microscopic.The result showed that common palm civet have a pair of anal gland which hasshaped like ball and located in the anal sacs wall’s in the ventrolateral of anus.Each anal gland has a ostium of anal canal dorsolaterally in the cutaneous zone of anal canal . Microscopic observation showed a well developed sebaceous gland and apocrine sweat gland in the wall of anal sac. Sebaceous and apocrine glands were excreted their product into main ductus to the anal canal.

Assessment of bias in morphological identification of carnivore scats confirmed with molecular scatology in north-eastern Himalayan region of Pakistan.

Scats are often used to study ecological parameters of carnivore species. However, field identification of carnivore scats, based on their morphological characteristics, becomes difficult if many carnivore species are distributed in the same area. We assessed error rates in morphological identification of five sympatric carnivores’ scats in north-eastern Himalayan region of Pakistan during 2013–2017. A sample of 149 scats were subjected to molecular identification using fecal DNA. We used a confusion matrix to assess different types of errors associated with carnivore scat identification. We were able to amplify DNA from 96.6% (n = 144) of scats. Based on field identification of carnivore scats, we had predicted that out of 144 scats: 11 (7.6%) scats were from common leopard, 38 (26.4%) from red fox, 29 (20.1%) from Asiatic jackal, 37 (25.7%) from yellow throated martin, 14 (9.7%) from Asian palm civet and 15 (10.4%) from small Indian civet. However, molecular identification revealed and confirmed nine were scats (6.24%) from common leopard, 40 (27.8 %) from red fox, 21 (14.6%) from Asiatic jackal, 45 (31.25%) from Asian palm civet, 12 (8.3%) scats from small Indian civet, while 11 scats (7.6%) were found from Canis lupus Spp., three (2%) from dog, one (0.7 %) scat sample from porcupine, and two (1.4%) from rhesus monkey. Misidentification rate was highest for Asian palm civet (25.7%), followed by red fox (11.1%) and Asiatic jackal (9.7%) but least for common leopard scats (4.2%). The results specific to our study area concur with previous studies that have recommended that carnivore monitoring programs utilize molecular identification of predator scats. Using only morphological identification of scats can be misleading and may result in wrong management decisions.

The Detection and Quantification of Adulteration in Ground Roasted Asian Palm Civet Coffee Using Near-Infrared Spectroscopy in Tandem with Chemometrics

In the present research, an evaluation of using near infrared (NIR) spectroscopy in tandem with full spectrum partial least squares (FS-PLS) regression for quantification of degree of adulteration in civet coffee was conducted. A number of 126 ground roasted coffee samples with degree of adulteration 0-51% were prepared. Spectral data were acquired using a NIR spectrometer equipped with an integrating sphere for diffuse reflectance measurement in the range of 1300-2500 nm. The samples were divided into two groups calibration sample set (84 samples) and prediction sample set (42 samples). The calibration model was developed on original spectra using FS-PLS regression with full-cross validation method. The calibration model exhibited the determination coefficient R2=0.96 for calibration and R2=0.92 for validation. The prediction resulted in low root mean square error of prediction (RMSEP) (4.67%) and high ratio prediction to deviation (RPD) (3.75). In conclusion, the degree of adulteration in civet coffee have been quantified successfully by using NIR spectroscopy and FS-PLS regression in a non-destructive, economical, precise, and highly sensitive method, which uses very simple sample preparation.

Sensory and Microbial Characteristics of Civet Coffee

Civet coffee has been known as the most expensive coffee in the world. Its high prices are mainly contributed by its uncommon production method. Civet coffee is produced from the coffee beans which have been digested by Asian palm civet or Luwak ( Paradoxorus hermaprodites ). Civet coffee production is very limited due to its processing is merely depends on civet biologic system. Unfortunately, reliable information on civet microbial digestive is still limited. The aim of this research is to evaluate cup (sensory) and microbial quality of wild and caged civet coffee. Samples of wild and caged civet coffee obtained from Bener Meriah District, Aceh, Indonesia. Sensory quality parameters consist of fragrance, aroma, flavour, aftertaste, acidity, body, balance, uniformity, clean cups, sweetness and overall. Microbial quality was done by enumeration of lactic acid bacteria. The results showed that wild and caged civet coffee relatively had similar profile sensory and microbial characteristics. The score cupping of wild civet coffee is slightly higher than caged civet coffee. Aroma and flavour characteristics of wild and caged civet coffee were herby, nutty, grassy tobacco-like, fishy and sweet but there were over fruity-papaya and guava-like aroma and flavour of caged civet coffee. The lactic acid bacteria count on wild and caged civet were 5.8 x 10 8 cfu/ml and 3,8 x10 8 cfu/ml. Three different colonies from MRS media were isolated and submitted to gram and catalase test. All isolates showed gram-positive and catalase negative. The result confirmed that most colonies are lactic acid bacteria group. Two isolates (ICF 1 and ICF 3) showed proteolytic activity, and isolated ICF 1 showed the largest zone of clearance of 15 mm. Further investigation is required for identification and selection of performance lactic acid bacteria for artificial civet coffee fermentation.

<b>Small carnivores of Wayanad Wildlife Sanctuary, the southern Western Ghats, India</b>

The small carnivores of Wayanad Wildlife Sanctuary were surveyed using camera traps. The study was conducted between November 2016 and February 2017 at 111 camera trap locations. Twenty-five mammal species were recorded, including nine small carnivores from the Mustelidae, Viverridae, Herpestidae and Felidae families. Species included the Asian Small-clawed Otter Aonyx cinereus, Brown Palm Civet Paradoxurus jerdoni, Common Palm Civet Paradoxurus hermaphroditus, Small Indian Civet Viverricula indica, Indian Grey Mongoose Herpestes edwardsii, Ruddy Mongoose Herpestes smithii, Stripe-necked Mongoose Herpestes vitticollis, Jungle Cat Felis chaus and Leopard Cat Prionailurus bengalensis. The most common small carnivore was Viverricula indica, followed by Herpestes vitticollis and Paradoxurus hermaphroditus. Time activity patterns revealed that the viverrids were nocturnal while the herpestids were diurnal. The sanctuary supports one endemic species of small carnivore, Paradoxurus jerdoni, and one threatened species, Aonyx cinereus, which is Vulnerable according to the IUCN Red List of Threatened Species. This documentation highlights the biodiversity significance of Wayanad Wildlife Sanctuary and the Nilgiri mountain range in the Western Ghats.

The complete mitochondrial genome of Asian palm civet (Paradoxurus hermaphroditus) with phylogenetic consideration

Asian palm civet (Paradoxurus hermaphroditus) is one of the smallest palm civet which is least studied. Here, we report the first complete mitochondrial (mt) genome of Asian palm civet (P. hermaphroditus). The circular mt genome with a length of 16,706 bp contained 1 control region, 2 rRNAs, 13 protein-coding genes, and 22 tRNAs. Overall base composition of the complete mt DNA was 33.7% A, 30.5% T, 22.9% C, and 12.9% G. All the genes in mt genome of Asian palm civet (P. hermaphroditus) were distributed on the H-strand, except ND6 and eight tRNA genes encoded on the L-strand. Maximum likelihood (ML) and Bayesian inference (BI) methods were used to infer the phylogenetic relationship of P. hermaphroditus. The phylogenetic analysis shows that all species from the family Viverridae cluster together, in which P. hermaphroditus exhibits the closest relationship with P. larvata.

Physical Land Suitability for Civet Arabica Coffee: Case Study of Bandung and West Bandung Regencies, Indonesia

Indonesia has been widely known as the best Arabica coffee (Coffea arabica) producer, in terms of both aspects, quality and number of product. Currently, its production, however, declines to the 3rd rank internationally. Issues emerged in the coffee cultivation are: land unsuitability, low quality of seeds, and poor management. Among Arabica coffee types, wild civet coffee is the most expensive one and harvested from the coffee beans which have been digested naturally. The study aims to determine the physical suitability of land as well as the constraints related to land for civet Arabica coffee in selected study cases, e.g., Bandung and Bandung Barat. The research methods employ multi-criteria analysis, and combined with weighted overlaying techniques for mapping. The criteria include temperature, rainfall, humidity, duration of dry season, slope, altitude, type of soil, soil texture, and erosion potential. Parameters of civet (Paradoxurus hermaphroditus) are land use, altitude, and temperature. Local policy strongly supports the extensive management for land and the increase of coffee export. Processing data involved matching the comparison between guideline requirements for the land suitability classes, characteristics of Arabica coffee and civet habitat. The results covered the profile suitable land of the civet Arabica coffee in the study areas.

<b>Small carnivores of the montane forests of Eravikulam National Park in the Western Ghats, India</b>

The study of small carnivores of the montane forests of Eravikulam National Park in the southern Western Ghats, India recorded nine species of small carnivores using the camera trapping technique. These include three species of Mongoose (Herpestidae), two species each of lesser cats (Felidae) and civets (Viverridae), one species each of Otter and Marten (Mustelidae). They are Stripe-necked Mongoose Herpestes vitticollis, Brown Mongoose Herpestes fuscus, Indian Grey Mongoose Herpestes edwardsii, Jungle Cat Felis chaus, Leopard Cat Prionailurus bengalensis, Common Palm Civet Paradoxurus hermaphrodites, Small Indian Civet Viverricula indica, Asian Small-clawed Otter Aonyx cinereus, and Nilgiri Marten Martes gwatkinsii. It is interesting to note that the felines (lesser cats) are the more common small carnivores in the montane forests. Felis chaus was the most abundant small carnivore, which is followed by Prionailurus bengalensis and Herpestes vitticollis. Two species are Vulnerable as per the IUCN Red List, viz., Martes gwatkinsii and Aonyx cinereus.

Growth characteristics of common palm civets (Paradoxurus hermaphroditus Pallas, 1777) in captivity

The results of this study raveal the growth characteristics of civet (Paradoxurus hermaphroditus Pallas 1777) in captivity. This study was carried out in Dong Nai Biotechnology Center and Thanh Long Wildlife Farm (Thu Duc District, Ho Chi Minh City) from June, 2015 to June, 2017. The results showed that the civet with an average weight gain of 3 to 24 months was 5,14 g/head/day (for male civets) and 4,71 g/head/day (for female civets). The highest growth rate was from 9 -12 months old. The growth rate of the body length tends to decrease over the months. Growth stage of the body length was fastest in the period of 3-6 months with an absolute growth rate of 2,87 cm/head /month (for male civets) and 2,57 cm/head/month (for female civets). The average of tail length growth rate was 0,95 cm/head/month (for male civets) and 0,93 cm/head/month (for female civets). The absolute growth rate of the average breasts was 0,45 cm/head/month (for male civets); 0,39 cm/head /month (for female civets) and reached the highest in the 9 month period. The coefficient of variation of mass (period 9-24 months), tail length, body length and breasts of civet were in the high stability group (Cv% <10%).

<b>Winter food habits of the Golden Jackal <I>Canis aureus</I> (Mammalia: Carnivora: Canidae) in Patna Bird Sanctuary, Uttar Pradesh, India</b>

Golden Jackal (Canis aurius indicus) survives in a wide range of environment. Its foraging adaptation varies according to quality and abundance of food sources. The food habits also fluctuate according to season and habitat. This study investigated the diet composition of Golden Jackal using scat analysis method collected between November 2012 and April 2013 from Patna Bird Sanctuary (PBS), Uttar Pradesh, India. A total of 83 scats of Golden Jackal were collected and analysed. Sixteen food items were identified in Golden Jackal scats. Birds contributed maximum (ca. 38.92%) in Golden Jackal diet followed by rodents (ca. 12.14%), insects (ca. 8.92%), reptile (ca. 8.57%), vegetative matter (ca. 13.56%), rufous-tailed hare (ca. 6.07%), cattle (ca. 2.5%), squirrel (ca.1.42%), nilgai (ca.1.07%) and common palm civet (ca. 0.71 %). However (ca. 6.07 %) of prey items could not identified in Golden Jackal scats. Out of (ca. 38.92%) in bird’s contribution egret alone contributed about (ca.20.35%) in Golden Jackal diet followed by gargeny (ca. 8.93%), greater caucal (ca. 5.71%), babbler (ca.1.07%) and (ca. 2.85%) remains of birds were could not identified as per species level. This study is conducted to find out food habits of Golden Jackal and its impact on bird community structure.

<b>Wildlife hunting by indigenous people in a Philippine protected area: a perspective from Mt. Apo National Park, Mindanao Island</b>

Indigenous people play a major role in the conservation of wildlife species in protected areas. This paper provides an insight on the involvement of indigenous people in protecting and hunting of wildlife species in Mt. Apo National Park, Mindanao Island, Philippines. Here, I assessed indigenous knowledge and practices towards wildlife hunting from three villages from Mt. Apo through immersions, interviews, and focus group discussion between May 2014 and January 2015. The majority of wildlife hunters I encountered were male and married between the ages of 35–60 years old. Commonly hunted wildlife species from the national park included large wildlife species such as the endemic and threatened Philippine Warty Pig Sus philippensis, Philippine Brown Deer Rusa marianna, Common Palm Civet Paradoxurus hermaphroditus, Reticulated Python Malayopython reticulatus, Common Monitor Lizard Varanus salvator and other large birds such as Rufous Hornbills Buceros hydrocorax, large dove species (i.e., Dacula spp.). In the past, wildlife hunting was most commonly done for sustenance and culture. But, poverty and the lack of alternative livelihoods have become a recent motivation to hunt wildlife. This current findings in this study suggest that wildlife are essential for indigenous people in protected areas, however, hunting practices should be monitored and provide alternative livelihood options to reduce threats. This study introduced the vital links between local communities and wildlife in protected areas. Thus, engaging and empowering indigenous people and local communities in wildlife protection combined with appropriate conservation planning are the first steps forward in attaining sustainable and effective local conservation in protected areas.

KEPADATAN POPULASI MAMALIA DARAT KARNIVORA DI CAMP LEAKEY KAWASAN TAMAN NASIONAL TANJUNG PUTING, KALIMANTAN TENGAH

Borneo has wide land that support high biodiversity. One of them is Tanjung Puting National Park (TPNP), which have biodiversity such as terrestrial mammalian carnivore. Carnivore has a role to maintain its ecosystems. But, there are no data for population density of terrestrial mammalian carnivore. The object of this research is to find out population density of terrestrial mammalian carnivore in Camp Leakey, TPNP, Central Borneo. This research accomplished in September-October 2015 in Camp Leakey. Using line-transect sampling. Data collection was accomplished at 18.00-24.00 Central Indonesian Time (WITA) on eight transects with three times replication by direct surveys and indirect surveys. This research has obtained five species, malayan sun bear (Helarctos malayanus), sunda clouded leopard (Neofelis diardi), leopard cat, and group of civet, like small-toothed palm civet (Arctogalidia trivirgata) and asian palm civet (Paradoxurus hermaphroditus). Population density of each species from the highest to the lowest is 13,5 Individual of leopard cat/km2, 9,84 Individual of malayan sun bears/km2, 4,31 Individual of sunda clouded leopard/km2, and 3,65 Individual of civet/km2. Malayan sun bears, sunda clouded leopards and civets prefer to be in land forest. Leopard cats prefers to be in transition forest.

Free-ranging livestock influence species richness, occupancy, and daily behaviour of wild mammalian species in Baluran National Park, Indonesia

In some developing countries, human activities in protected areas threaten wildlife populations and their habitats. This study was conducted to understand the influences of free-range livestock on the wild mammalian population in Baluran National Park, Indonesia. There were 3852 and 1156 cows and goats, respectively, and livestock occupied an area of approximately 55.96km2. The species richness and probability of occupancy in areas with and without livestock were assessed, and the spatial co-occurrence and overlap of daily activity patterns among mammalian species and livestock were measured. A total of 39 camera traps were installed from August 2015 to January 2016, collecting 23,021 independent photographs. In areas with livestock, the number of mammal species (eight) was lower than in areas without livestock (11 species). The most affected species were the large herbivores Bubalus bubalis and Bos javanicus, and a large carnivore Panthera pardus, all of which were absent in areas with livestock. Regardless of the feeding guild, the probability of occupancy of almost all species declined in areas with livestock, except the medium herbivores Paradoxurus hermaphroditus and Hystrix javanica that showed a higher probability of occupancy. The species whose probability of occupancy declined were the carnivores Cuon alpinus and Prionailurus bengalensis; the herbivores Rusa timorensis, Tracypithecus auratus and Muntiacus muntjak; and the omnivore Sus scrofa. In the presence of livestock, R. timorensis and S. scrofa changed their activities from diurnal to nocturnal. Livestock affected most wild mammals in several ways, including by reducing the species richness, lessening the probability of occupancy and changing the daily activity patterns of many animals. This research recommends a significant reduction in the size of the range area for domestic livestock.

Hematology and serum biochemistry parameters for rescued common palm civets (Paradoxurus hermaphroditus) in different age groups.

Currently, there are no complete parameters established for serum biochemistry and hematology for the determination of health status of rescued common palm civets (Paradoxurus hermaphroditus). In this study, blood samples were obtained from 18 adults and 15 juvenile civets caught on Singapore Main Island. Significant age-related differences (P<0.05) were noted in the hemoglobin, erythrocyte count, packed cell volume (PCV), total serum protein and globulin concentration in the adult civets showing higher values compared with the juvenile civets. The mean corpuscular volume (MCV), the alkaline phosphatase (ALP) and the phosphorus concentrations were significantly higher (P<0.05) in juveniles compared with adult civets.

The Use of Partial Least Square Regression and Spectral Data in UV-Visible Region for Quantification of Adulteration in Indonesian Palm Civet Coffee.

Asian palm civet coffee or kopi luwak (Indonesian words for coffee and palm civet) is well known as the world's priciest and rarest coffee. To protect the authenticity of luwak coffee and protect consumer from luwak coffee adulteration, it is very important to develop a robust and simple method for determining the adulteration of luwak coffee. In this research, the use of UV-Visible spectra combined with PLSR was evaluated to establish rapid and simple methods for quantification of adulteration in luwak-arabica coffee blend. Several preprocessing methods were tested and the results show that most of the preprocessing spectra were effective in improving the quality of calibration models with the best PLS calibration model selected for Savitzky-Golay smoothing spectra which had the lowest RMSECV (0.039) and highest RPDcal value (4.64). Using this PLS model, a prediction for quantification of luwak content was calculated and resulted in satisfactory prediction performance with high both RPDp and RER values.