Compensatory biofuel ash fertilisation is recommended for managed Pinus sylvestris L. forests growing on nutrients poor Arenosols in Lithuania. We conducted an experiment between 2002 and 2005, investigating the effects of biofuel ash and nitrogen fertilisation applied to Scots Pine stands on nutrient poor Arenosols. We studied the effects of three fertilization treatments (biofuel ash, nitrogen and ash applied together with nitrogen) on dry mass and concentrations of the main nutrients (N, P, K, Ca and Mg) and other elements (Cu, Zn, Cd, Cr, Ni, and Pb) in different compartments and layers of the canopy. The biofuel ash, nitrogen fertilizers and ash applied together with nitrogen led to heavier needles in the upper and middle layers of the canopy with less effect toward the canopy base. The complex ash plus nitrogen treatment gave the strongest significant response. All treatments resulted in an increase in dry mass, but not in the length of the current year needles. The additional input of nitrogen induced higher increase in dry mass compared to the changes of N concentration in the youngest needles. On the basis of the present study results, it can be concluded that the youngest needles produced new mass per amount of nitrogen more effectively compared to older needles.