Aseptic Abscesses Research Articles

Protein metabolism after injury with turpentine: a rat model for clinical trauma

Subcutaneous injections of turpentine induced discrete aseptic abscesses in rats without detectable injury to other tissues. Hypoalbuminemia and high circulating concentrations of alpha 2-macroglobulin were present after 48 h. Liver size, protein content, and protein fractional synthetic rate (FSR) were all increased, whereas loss of muscle protein occurred together with similar (45-50%) reductions in both intramuscular glutamine concentrations ([Gln]i, mmol/l intracellular water) and FSR. The rats became anorexic, but pair feeding (50% ad libitum for 48 h) produced a 22% increase in muscle [Gln]i and only an 18% reduction in muscle FSR. Dietary restriction therefore did not demonstrate the positive relationship between muscle [Gln]i and FSR, which has been observed in animal trauma studies. It is concluded that subcutaneous injections of turpentine produce many of the features of the acute-phase response to injury, and therefore this technique is recommended as a convenient laboratory model for studying various aspects of the protein metabolic response to injury.

In vivo elaboration of CSF in acute inflammation: Proportionality to the intensity of the inflammatory stimulus and requirement of T lymphocytes

The colony-stimulating factor(s) (CSF) that stimulates the in vitro growth of bone marrow granulocyte-monocyte progenitors (CFU-GM) increases in the serum of mice challenged by an aseptic abscess induced by copper rod insertion. The effect of the inflammation on the increase of serum CSF is dose-related. The creation of 3 aseptic abscesses indeed results in a higher and longer elevation of serum CSF than 1 abscess. Serum CSF also increases in parallel with the rise in bone marrow CFU-GM; this is consistent with the CSF playing a role in regulation of haematopoiesis in vivo. From previous studies, it appears that T lymphocytes play a central role in the regulation of haematopoiesis. In order to determine the role of T lymphocytes in the inflammation response, cyclosporin A (CyA), an inhibitor of T lymphocyte function, was given in vivo, 2 days before inflammation induction. CyA abrogates the increase in both serum CSF and CFU-GM. Furthermore, a lower increase in serum CSF was observed in copper-implanted nude mice. These results suggest that the CSF production induced by inflammation requires the functional integrity of T lymphocytes.

In vivo stimulation and inhibition of granulopoiesis: the effect of an inflammatory reaction on murine diffusion chamber granulopoiesis.

Humoral factors influencing granulopoiesis have been evaluated using diffusion chambers (DC) implanted in the peritoneal cavity of mice challenged by an aseptic abscess produced by the subcutaneous implantation of copper rods. This resulted in an increase in peripheral blood neutrophils and an increase in tibial granulocytic elements. When DC loaded with bone-marrow cells were implanted into mice stimulated the day before by an aseptic abscess significantly more CFU-s, CFU-c, proliferative and non-proliferative granulocytes were produced, as compared to DC implanted into control hosts. When DC were implanted 4-6 d after the induction of inflammation in mice a significant depression of DC granulopoiesis was observed. Levels of serum and DC fluid CSF and serum inhibitors of in vitro colony growth showed no correlation with DC myelopoiesis. The data show that mice undergoing an inflammatory reaction elaborate first humoral substance(s) enhancing CFU-s and granulocytic growth in DC and next inhibitory factor(s) of DC granulopoiesis.

Pyretotherapy in the Treatment of Gonorrhea by Inducing Aseptic Abscess

Pyretotherapy in the Treatment of Gonorrhea by Inducing Aseptic Abscess