Ascorbic Acid Sensor Research Articles

Assessing the electrochemical sensing behavior of manganese based inverse spinel towards ascorbic acid detection

In this research article, a non-enzymatic electrochemical ascorbic acid (AA) sensor using inverse spinel of manganese cobaltite (MCO) stabilized on GCE was evaluated. Investigation on the characteristics of the synthesized manganese cobaltite nanoparticles were acquired from XRD, UV, XPS, FTIR, SEM, and TEM characterization techniques. The principal results observed include, two linear ranges in the sensor’s dynamic range of detection from 5 μM - 1.5 mM and 1.5 mM - 4.44 mM with LOD and LOQ of 0.085 µM and 0.283 µM respectively. The anti-interference study indicated a restoration of 107.4% and 91.3% recovery amidst biological and tablet excipient molecules respectively. The recovery results obtained from the biological and pharmaceutical samples conclude a satisfactory validation of recovery from pharmaceutical samples of Vitamin-C tablets and drinks.

Novel Zn/Co–N co-doped carbon quantum dot-based “on-off-on” fluorescent sensor for Fe(III) and ascorbic acid

In this work, the metal (Zn, Co) and non-metal (N) co doped carbon quantum dots (Zn/Co-NCDs) was prepared by hydrothermal method, and characterized by FT-IR, TEM and XPS. It was found that bimetal doped could effectively improve the quantum yield (QY) of carbon quantum dots. The presence of Fe(III) could "turn off" the fluorescence signal, and the addition of ascorbic acid (AA) could "turn on" the fluorescence signal. An "on-off-on" fluorescent probe based on Zn/ Co-NCDs for Fe(III) and AA analysis was established. The liner ranges were 0.03–20.00 μg/mL and 0.69–20.00 μg/mL with the LODs of 0.010 μg/mL and 0.23 μg/mL, respectively. The method was successfully applied to the determination real samples with satisfactory results.

Water-stable nickel-based coordination polymer for selective and sensitive enhancing and quenching fluorescence sensing of ascorbic acid and acetylacetone

The novel Ni-based coordination polymer [Ni(H2edda) (bpe)] (CP 1) was successfully synthesized by employing 5,5′ (ethane-1,2-diylbis(oxy)) diisophthalic acid (H4edda) and 1,2-bis (4-pyridyl) ethylene (bpe) under hydrothermal conditions. Structural analysis indicates that CP 1 presents a 2-fold-interpenetrated two-dimensional (2 D) framework with a uninodal sql topology. Further investigation reveals that the newly obtained CP can be stable in water or even exposed in a certain acid/base aqueous solution (pH = 3–11) for a long period of time. Remarkably, CP 1 can further serve as a bifunctional sensor for ascorbic acid (AA) and acetylacetone (acac) in aqueous media according to fluorescence enhancing and quenching effects, respectively. Notably, both sensing processes are labeled with exceptional anti-interference property, high sensitivity and selectivity, wide linear range (0–50 μM for AA and 0.2–1.8 mM for acac) and low detection limit (1.37 nM for AA and 1.58 μM for acac). Theoretical simulations and experimental findings point out that in the process of AA detection by CP 1, the significant fluorescence enhancement could be caused by the photo-induced electron transfer (PET) process, while the inner filter effect (IFE) mechanism combined with PET should be responsible for the fluorescence quenching of CP 1 by acac.

Cerium/polyacrylic acid modified porphyrin metal-organic framework as fluorescence and photothermal sensor for ascorbic acid measurement

A fluorescence and photothermal dual-signal assay was developed for the determination of ascorbic acid (AA). Cerium/polyacrylic acid modified porphyrin metal-organic frameworks (PCN-224@PAA-Ce MOFs) exhibit oxidase-like activity and can effectively oxidize 3,3 ′, 5,5 ′-tetramethylbenzidine (TMB) from colorless substrates to blue substrates (oxTMB). The absorption spectrum of oxTMB overlaps with the emission spectrum of PCN-224@PAA-Ce, resulting in quenching of fluorescence emission of PCN-224@PAA-Ce. oxTMB, as an excellent photothermal agent, converts the optical signal into the thermal signal (temperature increasing) driven by 808 nm laser. In the presence of AA, the blue oxTMB was reduced to colorless TMB, which led to the fluorescence recovery of PCN-224@PAA-Ce and at the same time, the photothermal signal was changed with the temperature decreasing under the driving of 808 nm laser. The detection limits (LOD) of fluorescence/photothermal dual-mode AA sensor strategies were as low as 0.73 μM and 1.4 μM, respectively. Rapid analysis of ascorbic acid was accomplished with the present procedure, AA in vitamin C tablets and human serum samples were validated.

Cover Feature: Direct Laser Writing of Poly(furfuryl Alcohol)/Graphene Oxide Electrodes for Electrochemical Determination of Ascorbic Acid (ChemElectroChem 17/2022)

The Cover Feature illustrates the use of a biomass-derived polymer for the direct laser writing fabrication of carbon-based electrodes for a non-enzymatic electrochemical sensor of ascorbic acid. More information can be found in the Research Article by L. Fernandes Loguercio et al.

Reactive sputtering deposition of Co3O4 films and an evaluation of its use as an electrochemical sensor for ascorbic acid

Reactive sputtering deposition of Co3O4 films and an evaluation of its use as an electrochemical sensor for ascorbic acid

MnO2 nanosheet-assisted ratiometric fluorescent sensor for ascorbic acid based on Pyronin Y and thiamine

Ascorbic acid (AA) which can be supplemented by exogenous sources such as food and beverage, plays an important role in human health. Abnormal level of AA in human body usually leads to various diseases. A facile MnO2 nanosheet-assisted ratiometric fluorescent sensor for AA was constructed in this work. MnO2 nanosheet (MnO2NS) could not only greatly quench the fluorescence of Pyronin Y (PY) by inner filter effect (IFE) but also oxidize non-fluorescent thiamine (vitamin B1, VB1) to blue fluorescent thiochrome (oxVB1) by taking advantage of the oxidase-mimicking activity simultaneously. However, MnO2NS could be reduced to Mn2+ in the presence of AA, inhibiting the IFE and oxidase-mimicking activity. According to this principle, the fluorescence intensity of oxVB1 decreasing at 440 nm and the fluorescence of PY recovering at 560 nm. Under optimal conditions, the MnO2NS-PY-VB1 system showed a wide linear range towards AA in the range of 0.25–110 µM with a low detection limit reaching 0.1 µM. Furthermore, it was also applied for sensitive detection of AA in orange juice and human serum.

A novel fluorescence-scattering ratiometric sensor based on Fe-N-C nanozyme with robust oxidase-like activity

Numerous ratiometric fluorescence nanozyme sensors are developed, though, fluorescence-scattering ratiometric sensors based on nanozymes are hardly reported. First-order scattering (FOS) could be quenched due to inner filter effect of quenchers on scattering signals. Meanwhile, Second-order scattering (SOS) depended on FOS. Based on the principle, we designed a novel nanozyme sensor by coupling fluorescence (FL) and SOS. Specifically, Fe-N-C nanozyme (Fe-HCNP) was fabricated by doping Fe into carbon-nitrogen polymers via formamide condensations. Fe-HCNP possessed robust oxidase-like activity, effectively transforming O2 into ·O2- and ·OH. Then, Fe-HCNP and o-phenylenediamine (OPD) severed as a hybrid system to construct an FL-SOS ratiometric sensor for ascorbic acid (AA). Fe-HCNP with large size possessed strong SOS. After being added into the Fe-HCNP/OPD system, AA could fast be oxidized to dehydroascorbic acid (DAA) due to Fe-HCNP as oxidase mimics. Subsequently, fluorescent quinoxaline derivative (DFQ) was produced after condensation reactions between DAA and OPD, causing an increase in fluorescence. Meanwhile, the intensity of FOS was reduced because FOS peaks of Fe-HCNP overlapped absorption bands of DFQ, causing a decrease in SOS. The FL-SOS ratiometric sensor for AA showed outstanding advantages of high specificity and fast. Overall, this work provided a new strategy for nanozyme ratiometric sensors.

Condensation product of 5-bromosalicylaldehyde and aminophenol: Fluorescence sensor for ascorbic acid and AND Logic Gate

Condensation product ( L ) of 5-bromosalicylaldehyde and aminophenol has been synthesised and characterised. Fluorescence of L enhances by 23 times on interaction with Ce 3+ while it is quenched completely by Ce 4+ . Ascorbic acid (AA) is a well known strong reducing agent and this property has been used to act L :Ce 4+ adduct as a fluorescence “on” sensor for AA. AA reduces Ce 4+ into Ce 3+ and thereby increasing fluorescence of L due to the formation of L :Ce 3+ adduct. Molecules which generally coexist with AA viz. Cholesterol, Glucose, Sucrose and Dopamine found not to interfere. The interaction of L with Ce 3+ , Ce 4+ and subsequently with AA has been verified with cyclic voltammetry.

Functionalized Iron Oxide Nanostructures: Recent Advances in the Synthesis, Characterization, and Electrochemical Biosensor Applications

FBiosensors play a significant role in the process of early diagnosis of non-communicable disease. In the global market, biosensors are valued at over 25.5 billion USD in 2021. Screen Printed Electrodes were traditionally used for biomolecule detection, but due to their poor stability and low conducting properties, they have been replaced by Glassy Carbon Electrodes, which exhibit excellent stability and reusability, only few works have been reported in glucose detection. The objective of this review is to provide an overview on the synthesis iron oxide nanoparticles and their composites developed for the electrochemical applications by various research works. The iron oxide and their composites of suitable morphologies at nanoscale dimensions prepared by various methods and conditions were compiled. The characterization results of all the samples obtained by X-Ray Diffraction, Scanning Electron Microscopy, Fourier Transform Infrared Spectroscopy, and Raman Spectroscopy were discussed in detail. The sensing efficiency of nanoscale iron oxides, when they are functionalized/combined with carbon nanotube, polyamidoamine dendrimer, Cellulose Nanocrystals, Terephthalic acid, Polyaniline, etc. were discussed in detail. The requirement for the development of more suitable electrochemical sensors for glucose, ascorbic acid, dopamine, and uric acid were proposed. The most suitable functionality for the iron oxide to is proposed for the development of efficient electrochemical sensor. Finally, the review provides the summary of the iron oxide nanomaterials, current challenges, and future scope of electrochemical sensors.

Glassy Carbon Electrodes Decorated with HgO/CNT Nanocomposite and Modified with a Conducting Polymer Matrix for Enzyme‐Free Ascorbic Acid Detection

AbstractIn an alkaline solution, mercuric oxide decorated carbon nanotube nanocomposites (HgO.CNT NCs) were manufactured utilizing an uncomplicated wet‐chemical approach. Ultra‐Violet spectroscopy (UV‐Visible), Fourier Transform Infra‐red spectroscopy (FTIR), Powder X‐ray Diffraction (XRD), X‐ray Photo‐electron Spectroscopy (XPS), and Field Emission Scanning Emission Microscopy (FESEM), X‐ray Electron Dispersive Spectroscopy (XEDS) techniques were applied to examine the HgO.CNT NCs that had been prepared. Ascorbic acid sensor was developed upon light coating of HgO.CNT NCs on polished Glassy carbon electrode (GCE) modified with nafion (Nf). Analytical performance of the selected ascorbic acid sensor was achieved such as sensitivity, Limit of quantification (LOQ), Limit of detection (LOD), Linear dynamic range (LDR), extended reliability, interference effect experimentation, and actual sample inspection using a consistent current‐voltage approach. Calibration curve of the suggested sensor was observed to be linear (R2=0.9957) throughout a broad range of ascorbic acid concentrations (100.0 pM∼100.0 mM) at a voltage of 0.8 V. The calibration curve yielded analytical parameters such as sensitivity (632.91 pAμM−1cm−2), LOQ (255.0 pM), LOD (76.50 pM), and LDR (100.0 pM ∼1.0 mM) for the proposed ascorbic acid sensor (GCE/HgO.CNT NCs/Nf). The wet‐chemical synthesis of HgO.CNT NCs is a good development of nanocomposite‐oriented sensor advancement in medical sciences with non‐enzymatic detection of biological substances. This proposed sensor applied to selective ascorbic acid detection in biological samples and obtained logical outcomes.

Highly sensitive ascorbic acid sensors from EDTA chelation derived nickel hexacyanoferrate/ graphene nanocomposites

Significance of ascorbic acid (AA) determination has been increasing due to its importance in both human life and industrial applications. This work demonstrates the development of a highly sensitive non-enzymatic AA sensor by using Graphene/Nickel hexacyanoferrate nanocomposite (Gr/NiHCF). In this work, EDTA chelation strategy was used for the formation of homogeneously distributed NiHCF nanoparticles on graphene sheets. EDTA residue-supported pyramidal and spherical nanoparticles of NiHCF deposited on graphene sheets were used for the development of a highly sensitive AA sensor. The formation of EDTA residue supported NiHCF nanoparticles on graphene surface was confirmed by different characterization techniques (XRD, SEM, TEM, FTIR, XPS). Cyclic voltammetry (CV) of Gr/NiHCF nanocomposites confirmed the electrochemical sensing of AA by oxidation mechanism. The Sensitivity of the fabricated Gr/NiHCF based AA sensor as determined by amperometric characterization was found to be 7.029 µA µM−1 cm−2. The sensor fabricated in the present study shows much better sensitivity over other AA sensors reported in the literature. The LOD of the fabricated sensors was found as 0.25 µM. Repeatability, reproducibility, and interference studies of fabricated sensors reveal the high accuracy and selectivity of a fabricated sensor for AA measurement. Stability study of fabricated sensors showed excellent stability up to 30 days, indicating the potential usage of Gr/NiHCF for AA sensing. The real-time use of fabricated sensors in accurate determination of AA in samples like Vitamin C supplements and different fruit juices indicated their potential usage for AA measurement in real samples.

Development of a Ratiometric Fluorescence Sensor for Ascorbic Acid Based on Oxidase-mimicking Activity of CoOOH Nanoflake

Development of a Ratiometric Fluorescence Sensor for Ascorbic Acid Based on Oxidase-mimicking Activity of CoOOH Nanoflake

Ascorbic Acid Sensor Based on CdS QDs@PDA Fluorescence Resonance Energy Transfer

An ascorbic acid (AA) sensor was constructed based on the fluorescence resonance energy transfer (FRET) between CdS quantum dots (CdS QDs) and polydopamine (PDA) to detect trace AA sensitively. FRET occurred due to the broad absorption spectrum of PDA completely overlapped with the narrow emission spectrum of CdS QDs. The fluorescence of CdS QDs was quenched and in the “off” state. When AA was present, the conversion of DA to PDA was hindered and the FRET disappeared, resulting in the fluorescence of CdS QDs in an “on” state. Importantly, the degree of fluorescence recovery of CdS QDs displayed a desirable linear correlation with the concentration of AA in the range of 5.0–100.0 μmol/L, the linear equation is , and the detection limit is 1.16 μmol/L (S/N = 3, n = 9). There was almost no interference with common amino acid, glucose and biological sulfhydryl small molecules to AA. Trace amount of AA in vitamin C tablets were determined and satisfactory results were obtained; the recoveries were observed to be 98.01–100.7%.

Electrochemical Detection of Ascorbic Acid in Oranges at MWCNT-AONP Nanocomposite Fabricated Electrode.

Ascorbic acid (AA) is an essential vitamin in the body, influencing collagen formation, as well as norepinephrine, folic acids, tryptophan, tyrosine, lysine, and neuronal hormone metabolism. This work reports on electrochemical detection of ascorbic acid (AA) in oranges using screen-print carbon electrodes (SPCEs) fabricated with multi-walled carbon nanotube- antimony oxide nanoparticle (MWCNT-AONP) nanocomposite. The nanocomposite-modified electrode displayed enhanced electron transfer and a better electrocatalytic reaction towards AA compared to other fabricated electrodes. The current response at the nanocomposite-modified electrode was four times bigger than the bare electrode. The sensitivity and limit of detection (LOD) at the nanocomposite modified electrode was 0.3663 [AA]/µM and 140 nM, respectively, with linearity from 0.16–0.640 μM and regression value R2 = 0.985, using square wave voltammetry (SWV) for AA detection. Two well-separated oxidation peaks were observed in a mixed system containing AA and serotonin (5-HT); and the sensitivity and LOD were 0.0224 [AA]/µA, and 5.85 µΜ, respectively, with a concentration range from 23 to 100 µM (R2 = 0.9969) for AA detection. The proposed sensor outperformed other AA sensors reported in the literature. The fabricated electrode showed great applicability with excellent recoveries ranging from 99 to 107 %, with a mean relative standard deviation (RSD) value of 3.52 % (n = 3) towards detecting AA in fresh oranges.

Non-Enzymatic Ascorbic Acid Sensor Simultaneous Applied to Potentiometric and Amperometric Measurement

This study used copper oxide nanoparticles (CuO NPs) modified molybdenum trioxide (MoO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> ) film prepare non-enzymatic ascorbic acid (AA) sensor. We used polyethylene terephthalate (PET) as the substrate for the AA sensor. The properties of the device were examined using potentiometric and amperometric measurement approaches. Screen printing and radio frequency (R.F.) sputtering were used to fabricate the device and the advantages and disadvantages of the potentiometric and amperometric approaches were measured and recorded. The average sensitivity, coefficient of determination, effects of interference, limits of detection (LOD), reproducibility and response time of the device were compared to existing known devices. The copper oxide nanoparticles (CuO NPs) used for the study were produced in-lab by the green synthesis method, and were then used to modify the MoO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> film by simple instillation. The properties of the CuO NPs/MoO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> /Silver/PET sensor showed that both potentiometric and amperometric measurement methods were viable for AA solutions in a concentration range 7.81 μM-2 mM. This study demonstrates that even without enzymatic assistance, AA sensors can show excellent selectivity and sensing performance.

Novel Potentiometric Non-Enzymatic Ascorbic Acid Sensor Based on Molybdenum Oxide Film and Copper Nanoparticles

In this study, we prepared a non-enzymatic potentiometric ascorbic acid sensor based on molybdenum oxide (MoO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> ) and copper nanoparticles (CuNPs). Also, we prepared a potentiometric sensor by monitoring the voltage change between the sensor and ascorbic acid, and proposed the reaction mechanism between molybdenum oxide and ascorbic acid. It is relatively rare in the field of non-enzymatic ascorbic acid sensors. Molybdenum oxide is a relatively new material applied in ascorbic acid sensors. The application of molybdenum oxide film and copper nanoparticles to ascorbic acid sensors is a new research direction. We employed screen printing technology to prepare reference electrodes and conductive wires on the polyethylene terephthalate (PET) substrate, and we also used epoxy for packaging. In the ascorbic acid concentration range of <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$7.81 ~\mu \text{M}$ </tex-math></inline-formula> -2 mM, we measured the characteristics of sensors with the voltage-time (V-T) measurement system, namely the average sensitivity, linearity, response time, interference effects, limit of detection (LOD), and temperature effects. The performance of the ascorbic acid sensor was significantly improved by the modification of CuNPs.

Highly crystalline N and S co-doped carbon dots as a selective turn off–on sensor for Cr(vi) and ascorbic acid and a turn off sensor for metanil yellow

Highly crystalline N and S co-doped carbon dots (NS CDs) were synthesized by a hydrothermal method using mesaconic acid, ethylenediamine, and sulfuric acid as C, N, and S sources.

Novel Zn/Co-N Co-Doped Carbon Quantum Dot-Based 'On-Off-On' Fluorescent Sensor for Fe(III) and Ascorbic Acid

Novel Zn/Co-N Co-Doped Carbon Quantum Dot-Based 'On-Off-On' Fluorescent Sensor for Fe(III) and Ascorbic Acid

A Cd(ii)-organic framework as a highly sensitive and rapid fluorometric sensor for ascorbic acid in aqueous medium

A new Cd(ii) MOF was used as a fluorometric sensor for ascorbic acid in water, fruits, vegetables and human urine.