The effect of benzanthrone on the blood, adrenal and liver ascorbic acid levels in adult male guinea‐pigs has been investigated. Benzanthrone and ascorbic acid were administered intraperitoneally and orally in doses of 25 mg and 50 mg/kg body weight respectively. Benzanthrone alone caused significant decrease of ascorbic acid levels in the blood, adrenal and liver. Supplementation of ascorbic acid appreciably restored the blood ascorbic acid. However, adrenal and liver ascorbic acid levels were restored only to some extent. Histochemical examination of ascorbic acid in the adrenal revealed almost similar changes. The mortality rate due to benzanthrone toxicity (500 mg/kg) was lowered by 40% in non‐scorbutic as compared to scorbutic guinea‐pigs.