The culture of the paralarvae of merobenthic octopus species generally results in high mortalities with many attempts failing to rear the paralarvae through to juvenile stage. Progress has been made with higher survival for paralarvae cultures, but all successful paralarvae cultures rely heavily on decapod zoeae, which are labour intensive and costly to maintain in many instances. The development of an artificial pellet that is easily captured, can be rapidly consumed and fulfils the nutritional needs of octopus paralarvae would greatly advance octopus paralarvae culture. The aim of this study was to evaluate the effect of shape and size of artificial pellets on feeding behaviour for early Octopus tetricus paralarvae. This study tested the capture success of six different artificial pellets in a crossed experiment for size (i.e., small, medium and large), and shape (i.e., spherical or rectangular). Paralarvae were observed individually for up to 5 min with the number, size, shape and handling time of pellets captured by 2 days post-hatch O. tetricus paralarvae being recorded. A total of 105 out of 250 paralarvae captured one of the six artificial pellets with no difference between the two provided shapes for small and large pellets. In contrast, there was a strong preference for smaller artificial pellets with 57 out of 105 paralarvae capturing the small pellets. The handling times ranged from 1 to 485 s across all sizes and shapes of artificial pellets with shorter mean handling times for rectangular artificial pellets (mean of 13 ± 41 s) compared to spherical artificial pellets (mean of 31 ± 97 s), regardless of size. Overall, handling times were rather short (< 20 s) for the majority of (91%) of paralarvae that captured artificial pellets. The results show that pellet shape is mostly irrelevant to early O. tetricus paralarvae, but size is an important factor that must be considered. Moreover, the short handling times suggest a low palatability of the artificial pellets used in the current study, which will be addressed in future studies.
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